BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,341,061	C→A	13.0%	Y3* (TAC→TAA)	ymiB →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,341,061	0	C	A	13.0%	1010.7 / 86.8	331	Y3* (TAC→TAA)	ymiB	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (124/163); new base A (23/21); total (147/184)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

Only 100 of 332 total aligned reads displayed.

AACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATC  >  CP009273/1340913‑1341208

aaCATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAc                                                                                                                                                      >  2:1002866/1‑149 (MQ=255)

      gctgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGaaa                                                                                                                                                <  2:2923731/149‑1 (MQ=255)

       ctgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAAt                                                                                                                                               <  1:50950/149‑1 (MQ=255)

        tgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAAtt                                                                                                                                              >  1:3174703/1‑149 (MQ=255)

             tCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAAcgg                                                                                                                                         <  2:3884433/149‑1 (MQ=255)

              ccGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAAcggc                                                                                                                                        <  1:767421/149‑1 (MQ=255)

               cGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAAcggcg                                                                                                                                       <  1:1654737/149‑1 (MQ=255)

                 tCAACCGCCAGTATGGATTGTGCTATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACggcggc                                                                                                                                     <  1:315337/149‑1 (MQ=255)

                  cAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCt                                                                                                                                    >  1:4560479/1‑149 (MQ=255)

                    aCCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTAAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTaa                                                                                                                                  >  1:2261878/1‑149 (MQ=255)

                       gCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACg                                                                                                                               >  2:964384/1‑149 (MQ=255)

                       gCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACg                                                                                                                               >  1:4303719/1‑149 (MQ=255)

                       gCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACg                                                                                                                               >  1:1913476/1‑149 (MQ=255)

                        ccAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGc                                                                                                                              <  1:1257463/149‑1 (MQ=255)

                           gTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAtt                                                                                                                           >  2:3210944/1‑149 (MQ=255)

                           gTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAtt                                                                                                                           >  2:572469/1‑149 (MQ=255)

                           gTATGGAGTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAtt                                                                                                                           <  2:1543799/149‑1 (MQ=255)

                            tATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTg                                                                                                                          >  2:4476851/1‑149 (MQ=255)

                            tATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTg                                                                                                                          >  1:3910010/1‑149 (MQ=255)

                            tATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAGTg                                                                                                                          >  2:3070243/1‑149 (MQ=255)

                              tGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑tt                                                                                                                       <  2:1280505/149‑1 (MQ=255)

                                gATTGTGCGATCCGAGAAAGTTGTACAACGTTGAGGACTCACTAACGGTTTTCATGATGCATATCCTCAGGTTAACAGCGGAGTCCGAGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAAAGGCGGCTAAACGCATTG‑tttt                                                                                                                     >  1:916796/1‑149 (MQ=255)

                                  ttGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑tttttt                                                                                                                   <  1:2742493/149‑1 (MQ=255)

                                   tgtgCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTG‑ttttttt                                                                                                                  <  1:4716489/149‑1 (MQ=255)

                                    gtgCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTACCGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑tttttttt                                                                                                                 <  1:1849351/149‑1 (MQ=255)

                                      gCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTtat                                                                                                               >  2:3169614/1‑149 (MQ=255)

                                         aTCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTtatata                                                                                                            >  2:1135615/1‑149 (MQ=255)

                                         aTCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTtatata                                                                                                            >  1:2479078/1‑149 (MQ=255)

                                          tCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTatatat                                                                                                           <  2:4720367/149‑1 (MQ=255)

                                           ccGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATAtatt                                                                                                          <  1:1768062/149‑1 (MQ=255)

                                           ccGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATAtatt                                                                                                          <  2:3946819/149‑1 (MQ=255)

                                             tagaAATTTGTAAAACGTTGTGGACTCCCTAACGGTTTTAATGATTCATATCCTCCGGTTAACAGCGTAGTCCGCGGTTAAATAAAAGTAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATAtattta                                                                                                        <  1:4019759/148‑1 (MQ=255)

                                              agaAAGTTGTACACCGTTGTGGACTCCCTACCGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATAtatttat                                                                                                       <  1:1702170/149‑1 (MQ=255)

                                              agaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATAtatttat                                                                                                       >  1:3812832/1‑149 (MQ=255)

                                               gaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATAtatttatt                                                                                                      <  1:2024749/149‑1 (MQ=255)

                                               gaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATAtatttatt                                                                                                      >  2:1691481/1‑149 (MQ=255)

                                               gaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATAtatttatt                                                                                                      <  1:4477371/149‑1 (MQ=255)

                                                aaaGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATAGCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATatttattt                                                                                                     <  2:2157409/149‑1 (MQ=255)

                                                 aaGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTg                                                                                                    <  2:3242891/149‑1 (MQ=255)

                                                      gTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATc                                                                                               <  1:2118334/149‑1 (MQ=255)

                                                          aaCGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGt                                                                                           >  2:490908/1‑149 (MQ=255)

                                                             ggtggcgACTCCCGAACGGTTGTCATGATGCATCTCCTCCGGTTAACCGCGGAGTCCGCGGTTAAATACAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGtttt                                                                                        <  2:4588221/143‑1 (MQ=255)

                                                             gTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGtttt                                                                                        <  1:2429970/149‑1 (MQ=255)

                                                             gTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGtttt                                                                                        >  1:4133672/1‑149 (MQ=255)

                                                             gTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGtttt                                                                                        <  1:3876871/149‑1 (MQ=255)

                                                              ttGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTg                                                                                       <  2:4231667/149‑1 (MQ=255)

                                                                     cTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAAc                                                                                <  1:4059300/149‑1 (MQ=255)

                                                                       cccTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGc                                                                              <  2:2392514/149‑1 (MQ=255)

                                                                         cTAACGTTTTTCATGATGAATAGCCTCCGGTTAACAGCGGAGGCCGCGTTTAAATAAACGGAAAAACATGACTTACATGAAATTAACGGCGGCTAAACGAATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCa                                                                            <  1:4397691/149‑1 (MQ=255)

                                                                         cTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCa                                                                            >  2:869118/1‑149 (MQ=255)

                                                                          tAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAg                                                                           >  2:4338997/1‑149 (MQ=255)

                                                                             cGGTTTTCATGATGCATATCCTCCGTTTAACAGCGGAGTCCGCGGTTAAATAAAAGCAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGttt                                                                        <  1:1123995/149‑1 (MQ=255)

                                                                              ggTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGtttt                                                                       >  1:375172/1‑149 (MQ=255)

                                                                               gTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTc                                                                      >  1:4258338/1‑149 (MQ=255)

                                                                               gTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTc                                                                      <  2:1844595/149‑1 (MQ=255)

                                                                                  ttCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCaaa                                                                   <  2:2888416/149‑1 (MQ=255)

                                                                                  ttCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCaaa                                                                   >  2:1920430/1‑149 (MQ=255)

                                                                                  ttCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCAAGTTTTGGAAAAACGCCAGTTTTCaaa                                                                   >  2:2987730/1‑149 (MQ=255)

                                                                                  ttCATGAGGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCaaa                                                                   <  2:200069/149‑1 (MQ=255)

                                                                                      tgatgCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGaa                                                               >  1:333695/1‑149 (MQ=255)

                                                                                        ttgCATAGCCTCCGTTTACCAGCGGAGTCCGCGGTTAAATAACAGGAACAACAGGACTTACATGAAATTAACGGCGGCTAAACGCTTTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAg                                                             <  2:4071857/148‑1 (MQ=255)

                                                                                         tgCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGt                                                            <  2:1463832/149‑1 (MQ=255)

                                                                                             tatCCTCCGGTTAACAGCGGAGTCCGCGGTTAAAAAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGt                                                        <  1:4322685/149‑1 (MQ=255)

                                                                                              atCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAACGTCAGtt                                                       >  2:3351842/1‑149 (MQ=255)

                                                                                               tcctccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTa                                                      >  1:2552759/1‑149 (MQ=255)

                                                                                                 ctccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaaa                                                    >  1:1169491/1‑149 (MQ=255)

                                                                                                  tccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGTAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaaaa                                                   <  1:662593/149‑1 (MQ=255)

                                                                                                  tccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaaaa                                                   >  1:618294/1‑149 (MQ=255)

                                                                                                   ccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaaaaa                                                  >  2:1038398/1‑149 (MQ=255)

                                                                                                    cGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaaaaaa                                                 <  2:4139220/149‑1 (MQ=255)

                                                                                                      gTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATc                                               >  1:4586611/1‑149 (MQ=255)

                                                                                                      gTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATc                                               <  2:83991/149‑1 (MQ=255)

                                                                                                        tAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTg                                             >  1:3505984/1‑149 (MQ=255)

                                                                                                            aGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTg                                         >  1:3280500/1‑149 (MQ=255)

                                                                                                            aGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTg                                         >  1:3684790/1‑149 (MQ=255)

                                                                                                            aGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTg                                         >  1:111905/1‑149 (MQ=255)

                                                                                                             gCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTgg                                        >  2:2617808/1‑149 (MQ=255)

                                                                                                                gAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGata                                     >  1:2098353/1‑149 (MQ=255)

                                                                                                                gAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTCTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGata                                     >  1:962983/1‑149 (MQ=255)

                                                                                                                 aGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGatat                                    >  2:3872819/1‑149 (MQ=255)

                                                                                                                 aGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGatat                                    <  1:2683044/149‑1 (MQ=255)

                                                                                                                  gTCCGCGTTTAAATAAAACGAACAACACGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGAtata                                   <  2:8341/149‑1 (MQ=255)

                                                                                                                  gTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGAtata                                   <  2:1716734/149‑1 (MQ=255)

                                                                                                                  gTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCGGGAtata                                   >  2:4493469/1‑149 (MQ=255)

                                                                                                                      gcgGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGa                               <  1:2389691/149‑1 (MQ=255)

                                                                                                                            aaataaaAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTag                         >  1:3191153/1‑149 (MQ=255)

                                                                                                                            aaataaaAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTag                         >  1:799909/1‑149 (MQ=255)

                                                                                                                            aaataaaAGGAACAAAATGACTTACATTAAATTAACGGCGGCTAAAAGCATTGTTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTa                          <  2:2032769/149‑1 (MQ=255)

                                                                                                                             aataaaACCAAAAACACCACTAACAATAAATCAACGGCGGCAAAACTCATTG‑TTTTTTTTATATATTTATTTGTAATCCATTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTaga                        <  1:2910794/149‑1 (MQ=255)

                                                                                                                               taaaAGGAACAACATTACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTagag                       <  2:3700962/149‑1 (MQ=255)

                                                                                                                                   aGGAACAACATGACTTACATTAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCgggg                  <  2:1301676/149‑1 (MQ=255)

                                                                                                                                   aGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCgggg                  <  1:305945/149‑1 (MQ=255)

                                                                                                                                   aGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCgggg                  >  1:3442338/1‑149 (MQ=255)

                                                                                                                                   aGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCgggg                  >  1:2974359/1‑149 (MQ=255)

                                                                                                                                    cgAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGa                 <  2:1632048/148‑1 (MQ=255)

                                                                                                                                          acaTGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATaaa           <  2:2176944/149‑1 (MQ=255)

                                                                                                                                           caTGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAAc          <  2:3751078/149‑1 (MQ=255)

                                                                                                                                            ttttcgtACATGAAATTAACGGCGGCTATACGCTTTG‑TTTTTTTTATATATTTCTTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGTGCGGGGATATAAACg         <  2:2692875/143‑1 (MQ=255)

                                                                                                                                            aTGACTTAAATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGAAAATAAACg         >  2:3405194/1‑149 (MQ=255)

                                                                                                                                                   aCATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAATCGGCccatc  >  2:2100294/1‑149 (MQ=255)

AACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATC  >  CP009273/1340913‑1341208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`