Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,386,008 G→T 100% Q35K (CAG→AAG)  ycjY ← S9 homolog non‑peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,386,0080GT99.2% 772.0 / ‑3.3 244Q35K (CAG→AAG) ycjYS9 homolog non‑peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (96/142);  total (100/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 245 total aligned reads displayed.

TAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTGATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT  >  CP009273/1385862‑1386154
                                                                                                                                                    |                                                                                                                                                   
 aaCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTATTTAAAGCCCCCACCTGGGTGTGAT‑AACAC‑AATTGCCTtata                                                                                                                                                  >  2:2224815/1‑149 (MQ=255)
  aCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATAc                                                                                                                                                 <  2:3102921/149‑1 (MQ=255)
  aCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATAc                                                                                                                                                 <  1:1993322/149‑1 (MQ=255)
   cTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACt                                                                                                                                                <  2:1981055/149‑1 (MQ=255)
     gACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑a                                                                                                                                             <  2:1885865/149‑1 (MQ=255)
     gACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑a                                                                                                                                             <  2:1255421/149‑1 (MQ=255)
      aCGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑Ac                                                                                                                                            <  2:4085744/149‑1 (MQ=255)
       cgcgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCCCCTGGGTGAGAT‑AACAC‑AATTGCCGTATACTG‑ACg                                                                                                                                           >  2:4472866/1‑149 (MQ=255)
       cgcgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCGTATACTG‑Acc                                                                                                                                           >  2:2155637/1‑148 (MQ=255)
       cgcgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGAGAT‑AACAC‑AAATTCCTTATACAG‑ACg                                                                                                                                           >  2:4553797/1‑149 (MQ=255)
          ggCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGTGAT‑AACAC‑ACTTGCCTTATACTG‑ACGGGt                                                                                                                                        >  2:4098374/1‑149 (MQ=255)
           gCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGtt                                                                                                                                       <  2:3772445/149‑1 (MQ=255)
           gCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGtt                                                                                                                                       >  2:1054767/1‑149 (MQ=255)
               gccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCAGGGTGTGAT‑AAAAC‑AAATGCCGTATAATT‑ACGGGGTtaat                                                                                                                                   >  1:404285/1‑149 (MQ=255)
                ccgccACTTTCACCTTGACAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTtcatc                                                                                                                                  <  1:3562937/149‑1 (MQ=255)
                  tcaaCTTTCACCTTTATAAGATGCGTCATAAGAAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCAACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATaaa                                                                                                                                <  2:2020443/146‑1 (MQ=255)
                   ccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCaaa                                                                                                                               >  2:4229973/1‑149 (MQ=255)
                     aCTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAAtt                                                                                                                             <  2:2798343/149‑1 (MQ=255)
                     aCTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAAtt                                                                                                                             <  2:2304242/149‑1 (MQ=255)
                     aCTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAAtt                                                                                                                             <  1:2801247/149‑1 (MQ=255)
                     aCTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGAT‑AACAC‑AAATGCCGTATACAG‑ACGGGTTTCATaaaaat                                                                                                                             >  2:50370/1‑147 (MQ=255)
                     aCTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGAGAT‑AACAC‑AAATTCCGTATACAG‑ACGGGTTTAATCAAAtt                                                                                                                             >  1:3092555/1‑149 (MQ=255)
                      cTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTACGCCCCCCCCCGGGGGAGTT‑AACACAAATTGCAT‑ATACTG‑ACGGGTTTAATCAAAatt                                                                                                                            >  1:1779615/1‑149 (MQ=255)
                          cACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCCCCAGGGTGTGAT‑AAAAC‑AAAATCCATATAATG‑AAGGGTTTAATCAAAATTTTGa                                                                                                                        >  1:1673763/1‑149 (MQ=255)
                           aCCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCACCTGGGTGAGAT‑AACAC‑AAATGCCTTATAATG‑ACGGGTTTAATCAAAtttttgtg                                                                                                                       >  1:1080729/1‑145 (MQ=255)
                               tGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGaa                                                                                                                   <  2:2834944/149‑1 (MQ=255)
                               tGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGTGAT‑AACAC‑AAATGCCTTATAATG‑ACGGGTTTCATCAAATTTTGGAGGGaa                                                                                                                   >  2:2265596/1‑149 (MQ=255)
                                gATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGaaa                                                                                                                  <  2:3942476/149‑1 (MQ=255)
                                gATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGaaa                                                                                                                  <  2:4788585/149‑1 (MQ=255)
                                   aaGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACCCCCCCACCAGGGTGAGAT‑AACAC‑AATTTCCGTATACTG‑ACGGGTTTAATCAAAATTTGGAGGGAAATAg                                                                                                               >  1:1553694/1‑149 (MQ=255)
                                      aTGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATc                                                                                                            <  1:870587/149‑1 (MQ=255)
                                       tGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAAAGCCCCCACCAGGGTGAGAT‑AACAC‑AATTGCCTTATAATG‑ACGGGTTTCATCAAATTTTTGTGGGAAATAGAtaa                                                                                                           >  1:802519/1‑147 (MQ=255)
                                       tGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTAAGCCCCCCCCCGGGTGTGGT‑TAAAC‑AAATTCCTTATAATT‑ACGGGGTTAATGAAAATTTGGTGGGGAatatataa                                                                                                           >  1:4087852/1‑147 (MQ=255)
                                        gCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCAc                                                                                                          <  1:4601862/149‑1 (MQ=255)
                                           tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCCCCGGGGTGAGAT‑AACAC‑CCATGCCGTATACTG‑ACGGGGGTAATCAAATTTTTGAGGGAAATAGATAACAGc                                                                                                       >  2:816906/1‑149 (MQ=255)
                                           tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGc                                                                                                       <  1:1377016/149‑1 (MQ=255)
                                            cATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGGTTTAAGCCCCCCCCGGGGGGTGCT‑AACAC‑AAATGCCCTATAATG‑TCGGGTTTCATAAAAATTTGGGGGGGAATAGATAACAGCa                                                                                                      >  1:91641/1‑149 (MQ=255)
                                              tAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGa                                                                                                    <  2:1779610/149‑1 (MQ=255)
                                              tAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGa                                                                                                    <  1:3940518/149‑1 (MQ=255)
                                                   aaTAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAag                                                                                               <  2:581503/149‑1 (MQ=255)
                                                     tAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAagag                                                                                             <  2:587871/149‑1 (MQ=255)
                                                     tAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAagag                                                                                             <  2:2979985/149‑1 (MQ=255)
                                                        tGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑AAGGGTTTCATCAAATTTTGGAGGGAAATATATCACTGCAGAAAAAGAGATg                                                                                          >  2:1018937/1‑149 (MQ=255)
                                                         gACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATgg                                                                                         <  1:2490225/149‑1 (MQ=255)
                                                         gACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAACGCCCCCACCGGGGGGAGAT‑TACAC‑AAATGCCGTTTACTG‑ACGGGTTTCAACAAATTTTGGTGGGAAATATATAACTGCAGACAAAGAGATgg                                                                                         >  1:55110/1‑149 (MQ=255)
                                                          aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTTTTATTTAACGCCCCCACCTTGGTGATAT‑AACAC‑AATTTCCTTAAACAG‑ACGGGGTTAATCAAAATTTTGATGGAAATATATAAATGCAGAAAAagataggtt                                                                                        >  2:3572885/1‑143 (MQ=255)
                                                          aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGt                                                                                        <  1:4216356/149‑1 (MQ=255)
                                                          aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGt                                                                                        <  1:4216320/149‑1 (MQ=255)
                                                                cccTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCAGGGTGAGAT‑AAAAC‑AAATTCCTTATACAG‑ACGGGTTTCATCAAAATTTGGAGGGAAATAGATAACTGCAGACAAAGAGATGGTTGGatt                                                                                  >  2:1311962/1‑149 (MQ=255)
                                                                     ttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGc                                                                             <  2:3952026/149‑1 (MQ=255)
                                                                     ttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGc                                                                             <  1:4391958/149‑1 (MQ=255)
                                                                         gCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAACCCCCCACCGGGGTGTGATAAAAAA‑AATTGCGT‑ATAAAGTACGGG‑TTCATAAAATTTTTGTGGGGAATAGATAAAGGCAGACAAAGAGGTTGTTGGGTTTTTTGTGtt                                                                         >  1:583641/1‑149 (MQ=255)
                                                                          ccAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCAGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTTGAGGGAAATAGATAACTGCAGACAAAGAGATGGGTGGATTATTTCTGTTa                                                                        >  2:4713212/1‑149 (MQ=255)
                                                                           cAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAg                                                                       <  1:70157/149‑1 (MQ=255)
                                                                                  ttGTCATAGGGTCCGGGCGTTTGTTCTTTATCGCCCCCACCTGGGTGAGTT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGTAATTGATCACTGCAGACATAGAGATGGTTGGATTATTGCTGTTAGTGAAGCt                                                                <  2:3423473/149‑1 (MQ=255)
                                                                                  ttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCt                                                                <  1:820053/149‑1 (MQ=255)
                                                                                    ggCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCAACTGGGTGAGAT‑AACAC‑AAATTCCATATACAT‑AAGGGTTTCATCAAATTTTGGAGGGAAAAATATCACAGCAGACAAATAGATTGTTGGATTATTTCTGTTATTTAAGCtta                                                              >  2:922377/1‑147 (MQ=255)
                                                                                     gCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGaaagagtg                                                             <  2:4637940/149‑7 (MQ=255)
                                                                                     gCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGaaagagtg                                                             <  2:4490725/149‑7 (MQ=255)
                                                                                      cATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACt                                                            <  1:340687/149‑1 (MQ=255)
                                                                                      cATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACt                                                            <  1:630184/149‑1 (MQ=255)
                                                                                        tAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACttt                                                          <  1:1141627/149‑1 (MQ=255)
                                                                                        tAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACttt                                                          <  1:3799284/149‑1 (MQ=255)
                                                                                        tAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGAT‑AACAC‑AATTTCCGTATACTG‑ACGGGTTTCAACAAAATTTTGAGGGAAATAGATAACAGCAGAAAAAGAGATGGTTGGATTATTGCTGGTATTGAAGCTGACttt                                                          >  2:762271/1‑149 (MQ=255)
                                                                                           gTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTttatt                                                       <  2:2105499/149‑1 (MQ=255)
                                                                                           gTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTttatt                                                       <  2:2494818/149‑1 (MQ=255)
                                                                                              ccGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTattatt                                                    <  1:1893272/149‑1 (MQ=255)
                                                                                                ctgttattGTTCATTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtca                                                  <  2:2941428/143‑1 (MQ=255)
                                                                                                  ccGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcatc                                                <  1:3010678/149‑1 (MQ=255)
                                                                                                    gTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTcatcat                                              <  2:1872280/149‑1 (MQ=255)
                                                                                                    gTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTcatcat                                              <  2:3919155/149‑1 (MQ=255)
                                                                                                          tCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGtt                                        >  1:1479586/1‑149 (MQ=255)
                                                                                                            tttAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATACCGTTcc                                      >  1:2387037/1‑149 (MQ=255)
                                                                                                              tAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTc                                    >  2:3495646/1‑149 (MQ=255)
                                                                                                                aCGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCAt                                  >  1:4448670/1‑149 (MQ=255)
                                                                                                                   cccccACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGa                               >  1:2154742/1‑149 (MQ=255)
                                                                                                                   cccccACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGa                               <  1:484599/149‑1 (MQ=255)
                                                                                                                   cccccACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGa                               <  1:728191/149‑1 (MQ=255)
                                                                                                                    ccccACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAg                              >  2:4403538/1‑149 (MQ=255)
                                                                                                                     cccACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGt                             <  2:3569848/149‑1 (MQ=255)
                                                                                                                         ccTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTc                         <  2:3883014/149‑1 (MQ=255)
                                                                                                                         ccTGGGTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTc                         <  1:116083/149‑1 (MQ=255)
                                                                                                                            gggTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGt                      <  2:2716732/149‑1 (MQ=255)
                                                                                                                             ggTGAGAT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGtt                     <  1:801652/149‑1 (MQ=255)
                                                                                                                                gagaT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGt                  >  1:2608917/1‑149 (MQ=255)
                                                                                                                                gagaT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGt                  >  1:2608797/1‑149 (MQ=255)
                                                                                                                                 agaT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGtt                 <  2:1592529/149‑1 (MQ=255)
                                                                                                                                  gaT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt                <  1:3666721/149‑1 (MQ=255)
                                                                                                                                  gaT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt                >  2:2384173/1‑149 (MQ=255)
                                                                                                                                  gaT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt                >  1:2270352/1‑149 (MQ=255)
                                                                                                                                  gaT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt                >  1:4497103/1‑149 (MQ=255)
                                                                                                                                  gaT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt                >  1:4667999/1‑149 (MQ=255)
                                                                                                                                   aT‑AACAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGtttt               >  2:3626832/1‑149 (MQ=255)
                                                                                                                                      aaCAC‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTaa             >  1:4705523/1‑149 (MQ=255)
                                                                                                                                       acac‑AATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAc            <  1:32091/149‑1 (MQ=255)
                                                                                                                                         ac‑aATTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACgg          <  1:4368485/149‑1 (MQ=255)
                                                                                                                                            aaTTGCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGtt        <  2:1716342/149‑1 (MQ=255)
                                                                                                                                                gCCTTATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTg    >  2:3329943/1‑149 (MQ=255)
                                                                                                                                                gCCTTAGACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGGTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTg    <  2:835064/149‑1 (MQ=255)
                                                                                                                                                  cTGATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGat  <  2:2567662/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTGATACTG‑ACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT  >  CP009273/1385862‑1386154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑