Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,424,234 C→A 100% S310* (TCA→TAA)  stfR → Rac prophage; putative tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,424,2340CA100.0% 1168.3 / ‑6.7 338S310* (TCA→TAA) stfRRac prophage; putative tail fiber protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (187/150);  minor base T (0/1);  total (187/151)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 348 total aligned reads displayed.

TTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGC  >  CP009273/1424088‑1424378
                                                                                                                                                  |                                                                                                                                                
ttCCTCGGCACCGGCGGCAGTAAATTCCGCTAGGGCGGAAAAAACGTCCGAGACTAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTaaa                                                                                                                                                <  1:1247498/149‑1 (MQ=255)
ttCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTaaa                                                                                                                                                <  1:270401/149‑1 (MQ=255)
gtCCTCGGCAACGGGGGCAGGAAATTCCGCGAAGGCGGCAAAACCGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTaaa                                                                                                                                                <  1:4574316/148‑1 (MQ=255)
  ccTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTaaaca                                                                                                                                              >  1:2063397/1‑149 (MQ=255)
   cTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTaaacaa                                                                                                                                             >  2:162765/1‑149 (MQ=255)
     cGGCACGGGCGGCTGGAATTTCCGCTCAGGCGGCAAAACCGGCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGt                                                                                                                                           <  1:4070637/149‑1 (MQ=255)
       gCAACGGCGGCTGTAATTTCCGCGACGGCGGAAAAAACGACCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGc                                                                                                                                         <  1:510636/149‑1 (MQ=255)
         aaCGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCg                                                                                                                                       >  2:1929958/1‑149 (MQ=255)
         aaCGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCg                                                                                                                                       >  2:3971008/1‑149 (MQ=255)
          aCGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCgg                                                                                                                                      <  1:4278415/149‑1 (MQ=255)
          aCGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCgg                                                                                                                                      >  1:2297312/1‑149 (MQ=255)
           cggcggCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCggg                                                                                                                                     >  1:3185447/1‑149 (MQ=255)
            ggcggcTGAAATTCCCGCTACGGCGCAAAAACCGCCCGAGACGAACCCCAGGTATTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGc                                                                                                                                    <  1:3604506/149‑1 (MQ=255)
            ggcggcAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGc                                                                                                                                    >  1:3338486/1‑149 (MQ=255)
            ggcggcAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGc                                                                                                                                    >  1:1437199/1‑149 (MQ=255)
             gcggcAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCa                                                                                                                                   <  2:1720321/149‑1 (MQ=255)
               ggcAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAgg                                                                                                                                 >  1:1162705/1‑149 (MQ=255)
                gcAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGc                                                                                                                                >  1:29760/1‑149 (MQ=255)
                gcAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGc                                                                                                                                >  1:3378846/1‑149 (MQ=255)
                  aGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCt                                                                                                                              >  1:234261/1‑149 (MQ=255)
                    taaaTTCCGCTAAGGCGGAAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCa                                                                                                                            <  1:553896/148‑1 (MQ=255)
                    gAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCa                                                                                                                            >  1:2366163/1‑149 (MQ=255)
                    gAAATTCCGCGAAGGCGGAAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCa                                                                                                                            <  2:3203319/149‑1 (MQ=255)
                      aaTTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGc                                                                                                                          >  1:4153172/1‑149 (MQ=255)
                      aaTTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGc                                                                                                                          >  1:1721846/1‑149 (MQ=255)
                        ttCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCa                                                                                                                        >  2:3429623/1‑149 (MQ=255)
                        ttCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCa                                                                                                                        <  2:4460122/149‑1 (MQ=255)
                         tCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAg                                                                                                                       >  1:2236305/1‑149 (MQ=255)
                              gAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCa                                                                                                                  >  1:4742706/1‑149 (MQ=255)
                                aGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCAcc                                                                                                                >  2:7525/1‑149 (MQ=255)
                                 ggcggcAAAAACGTCCGAGACGAACGCCTGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGTCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCAccg                                                                                                               <  1:3253227/149‑1 (MQ=255)
                                    ggcAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCAccgccg                                                                                                            >  1:3060473/1‑149 (MQ=255)
                                     gcAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCAccgccgc                                                                                                           <  1:1919869/149‑1 (MQ=255)
                                      aaaaacCGTCCTTGCCGAACGCCAGGTATTCTGAAAGGGCAGGGTGACTGAGCGCCTCGGCTGCGGCTGGATCAAAAACAGCGGCTGCGTCGTCTGACAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCAccgccgcc                                                                                                          <  1:4528762/148‑1 (MQ=255)
                                         aaaCGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGa                                                                                                       >  2:1406342/1‑149 (MQ=255)
                                           ccGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGaaa                                                                                                     <  1:2804624/148‑1 (MQ=255)
                                           aCGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGaaa                                                                                                     >  2:1224181/1‑149 (MQ=255)
                                              tCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCTAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATc                                                                                                  >  1:763876/1‑149 (MQ=255)
                                              tCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATc                                                                                                  >  2:555387/1‑149 (MQ=255)
                                              tCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATc                                                                                                  >  1:1963515/1‑149 (MQ=255)
                                              tCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATc                                                                                                  >  2:784645/1‑149 (MQ=255)
                                              tCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATc                                                                                                  <  1:1347097/149‑1 (MQ=255)
                                                     cGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACACGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGaa                                                                                           <  2:4771245/149‑1 (MQ=255)
                                                      gAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGaaa                                                                                          >  1:3686568/1‑149 (MQ=255)
                                                      gAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGaaa                                                                                          <  1:109171/149‑1 (MQ=255)
                                                       aaCGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAg                                                                                         >  2:3943740/1‑149 (MQ=255)
                                                       aaCGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAg                                                                                         >  2:1055800/1‑149 (MQ=255)
                                                       aaCGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAg                                                                                         <  1:1559073/149‑1 (MQ=255)
                                                        aCGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAgc                                                                                        >  2:1812142/1‑149 (MQ=255)
                                                           ccAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGcgcc                                                                                     >  2:1101767/1‑149 (MQ=255)
                                                           ccAGGTATTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAAAAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGcgcc                                                                                     <  1:4658289/149‑1 (MQ=255)
                                                              ggTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCa                                                                                  >  1:3288983/1‑149 (MQ=255)
                                                                  ttctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGt                                                                              >  2:2125840/1‑149 (MQ=255)
                                                                   tctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTc                                                                             >  1:3332755/1‑149 (MQ=255)
                                                                   tctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTc                                                                             <  2:707499/149‑1 (MQ=255)
                                                                    ctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCt                                                                            >  2:4720175/1‑149 (MQ=255)
                                                                     tGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTg                                                                           >  2:4290264/1‑149 (MQ=255)
                                                                      gAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGc                                                                          >  2:384798/1‑149 (MQ=255)
                                                                       aaaCGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCt                                                                         <  2:3728529/149‑1 (MQ=255)
                                                                          cGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCa                                                                      <  1:1876143/149‑1 (MQ=255)
                                                                           ggCCGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCaa                                                                     <  1:1678876/149‑1 (MQ=255)
                                                                                  ggACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCcac                                                              >  1:3584870/1‑149 (MQ=255)
                                                                                  ggACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCcac                                                              >  1:2753738/1‑149 (MQ=255)
                                                                                       gagCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGa                                                         >  1:3506554/1‑149 (MQ=255)
                                                                                           gcCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGc                                                     <  2:920667/149‑1 (MQ=255)
                                                                                           gcCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGc                                                     <  2:599512/149‑1 (MQ=255)
                                                                                           gcCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGc                                                     <  1:1031810/149‑1 (MQ=255)
                                                                                            ccTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCt                                                    <  1:25954/149‑1 (MQ=255)
                                                                                            ccTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCt                                                    >  2:2338481/1‑149 (MQ=255)
                                                                                            ccTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCt                                                    <  2:3586741/149‑1 (MQ=255)
                                                                                              tCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTgg                                                  <  1:3453293/149‑1 (MQ=255)
                                                                                                       gCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCAc                                         <  1:800926/149‑1 (MQ=255)
                                                                                                         aGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTg                                       <  2:4252974/149‑1 (MQ=255)
                                                                                                           gCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTACAACAGCCACAACGAAGGCTGGCGAAGCCACTGaa                                     >  2:3318550/1‑149 (MQ=255)
                                                                                                             tCAAAAACCGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACa                                   <  1:4489764/149‑1 (MQ=255)
                                                                                                              cAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAg                                  >  2:2018877/1‑149 (MQ=255)
                                                                                                               aaaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAgg                                 >  2:3099879/1‑149 (MQ=255)
                                                                                                                aaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGc                                <  2:4404365/149‑1 (MQ=255)
                                                                                                                  aaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCa                              >  2:2177441/1‑149 (MQ=255)
                                                                                                                  aaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCa                              >  2:4575755/1‑149 (MQ=255)
                                                                                                                        ggCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCgcag                        >  2:2622383/1‑149 (MQ=255)
                                                                                                                         gCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCgcagc                       >  1:3749578/1‑149 (MQ=255)
                                                                                                                           tGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGcagcag                     >  2:39212/1‑149 (MQ=255)
                                                                                                                            gCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCagcagc                    <  2:2911628/149‑1 (MQ=255)
                                                                                                                             cgtcgtCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCg                   <  2:2687212/149‑1 (MQ=255)
                                                                                                                              gtcgtcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGa                  <  1:2173086/149‑1 (MQ=255)
                                                                                                                               tcgtcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAg                 >  2:4398364/1‑149 (MQ=255)
                                                                                                                                 gtcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGt               <  1:148987/149‑1 (MQ=255)
                                                                                                                                  tcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTc              >  2:3674521/1‑149 (MQ=255)
                                                                                                                                  tcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTc              >  1:2535836/1‑149 (MQ=255)
                                                                                                                                      ccAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCt          <  1:1926411/149‑1 (MQ=255)
                                                                                                                                      ccAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCt          <  1:985675/149‑1 (MQ=255)
                                                                                                                                      ccAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCt          <  1:931652/149‑1 (MQ=255)
                                                                                                                                       cAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCtt         >  1:1069939/1‑149 (MQ=255)
                                                                                                                                       aaGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAATATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCtt         <  2:3505726/148‑1 (MQ=255)
                                                                                                                                        aGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTc        <  1:3942080/149‑1 (MQ=255)
                                                                                                                                        aGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTc        <  1:3943931/149‑1 (MQ=255)
                                                                                                                                              gCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGc  <  1:365278/149‑1 (MQ=255)
                                                                                                                                              gCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGc  >  1:2435123/1‑149 (MQ=255)
                                                                                                                                              gCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGc  >  1:2264619/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGC  >  CP009273/1424088‑1424378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑