Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,439,518 C→A 100% S737S (TCC→TCA)  ydbH → putative membrane‑anchored protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,439,5180CA99.4% 1071.0 / ‑2.1 316S737S (TCC→TCA) ydbHputative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (152/162);  total (153/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 316 total aligned reads displayed.

ACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGC  >  CP009273/1439372‑1439661
                                                                                                                                                  |                                                                                                                                               
aCTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAgg                                                                                                                                               <  1:3194920/149‑1 (MQ=255)
aCTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAgg                                                                                                                                               <  1:2179911/149‑1 (MQ=255)
  tGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTAGCCATGTCAgggg                                                                                                                                             >  1:2516772/1‑149 (MQ=255)
   gATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGc                                                                                                                                            >  2:1340736/1‑149 (MQ=255)
   gATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGc                                                                                                                                            <  1:1509842/149‑1 (MQ=255)
    aTGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCa                                                                                                                                           <  1:192679/149‑1 (MQ=255)
     tGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAt                                                                                                                                          >  2:1626437/1‑149 (MQ=255)
      gTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCAAGTCAGGGGCAtt                                                                                                                                         >  1:3841071/1‑149 (MQ=255)
            gTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAgtgg                                                                                                                                   <  1:4594936/149‑1 (MQ=255)
              cGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAgtggtg                                                                                                                                 <  2:3882155/149‑1 (MQ=255)
              cGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAgtggtg                                                                                                                                 <  2:3419225/149‑1 (MQ=255)
               gATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGc                                                                                                                                <  1:297688/149‑1 (MQ=255)
               gATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGc                                                                                                                                <  2:2792295/149‑1 (MQ=255)
                   tgtTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTg                                                                                                                            >  2:62730/1‑149 (MQ=255)
                   tgtTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTg                                                                                                                            >  1:500362/1‑149 (MQ=255)
                     ttAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGcc                                                                                                                          <  1:1234624/149‑1 (MQ=255)
                          cGGTAACGTACTGCTGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTAt                                                                                                                     <  1:2799745/149‑1 (MQ=255)
                          cGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTAt                                                                                                                     <  2:396456/149‑1 (MQ=255)
                           ggTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATg                                                                                                                    >  2:2001162/1‑149 (MQ=255)
                           ggTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCAGGTCAGGGGCATTTAGTGGTGCACTGCCGTTATg                                                                                                                    >  2:1919175/1‑149 (MQ=255)
                            gTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATgg                                                                                                                   >  1:2019750/1‑149 (MQ=255)
                             tAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGc                                                                                                                  <  1:445014/149‑1 (MQ=255)
                             tAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGc                                                                                                                  <  1:3649921/149‑1 (MQ=255)
                               aCGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTg                                                                                                                >  2:1777028/1‑149 (MQ=255)
                                cGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGa                                                                                                               <  2:1708272/149‑1 (MQ=255)
                                    cTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACaa                                                                                                           <  2:3708433/149‑1 (MQ=255)
                                     tgatgaAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAAt                                                                                                          >  1:2438515/1‑149 (MQ=255)
                                       atgaAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGa                                                                                                        >  1:4008293/1‑149 (MQ=255)
                                        tgaAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaa                                                                                                       >  2:4162875/1‑149 (MQ=255)
                                        tgaAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaa                                                                                                       >  2:4649644/1‑149 (MQ=255)
                                        tgaAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaa                                                                                                       >  2:3861162/1‑149 (MQ=255)
                                           aaCAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaaaaa                                                                                                    <  2:920845/149‑1 (MQ=255)
                                           aaCAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaaaaa                                                                                                    >  2:1397391/1‑149 (MQ=255)
                                            aaaaTTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAAt                                                                                                   <  2:3033878/147‑1 (MQ=255)
                                            aCAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAAt                                                                                                   <  1:2229588/149‑1 (MQ=255)
                                              aaTTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAGTTAGTGGTGCACTGCCGTTCTGGCTGAACACTGAAAAATgg                                                                                                 >  2:3226803/1‑149 (MQ=255)
                                               aatACGTATGCCGCAACATGACCCGTCGCTGTAGCGGCTGAATAATCTCACATCCAGCTAACTGGATAGCGCCGTCAATACGAAACAAATCGACATGTCATGGGCATTTAGATGTGCACTGCCGATATGGCTGAACAAACAAAAATgca                                                                                                >  2:4784852/3‑147 (MQ=255)
                                               aTTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGa                                                                                                >  1:3345392/1‑149 (MQ=255)
                                                ttACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGAt                                                                                               >  2:3643206/1‑149 (MQ=255)
                                                  aCGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATtg                                                                                             <  1:999509/149‑1 (MQ=255)
                                                    gTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATtgtg                                                                                           >  1:4283118/1‑149 (MQ=255)
                                                       tGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGaaa                                                                                        <  2:4051633/149‑1 (MQ=255)
                                                        gCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAg                                                                                       >  2:3106195/1‑149 (MQ=255)
                                                         ccGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGa                                                                                      <  1:1187877/149‑1 (MQ=255)
                                                          cGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAt                                                                                     <  2:3988480/149‑1 (MQ=255)
                                                           gCAACATGACCCGGCGCTGTTGCGGCTGAATACTCTCTCATCCAGTGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtg                                                                                    <  1:2732064/149‑1 (MQ=255)
                                                           gCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtg                                                                                    >  1:4177602/1‑149 (MQ=255)
                                                            cAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtgg                                                                                   <  1:3742147/149‑1 (MQ=255)
                                                                 tGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggc                                                                              >  1:865791/1‑149 (MQ=255)
                                                                  gACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggct                                                                             <  1:49079/149‑1 (MQ=255)
                                                                   aCCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggctg                                                                            <  1:1114047/149‑1 (MQ=255)
                                                                    cccGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggctgg                                                                           <  1:134346/149‑1 (MQ=255)
                                                                    cccGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggctgg                                                                           <  1:3144041/149‑1 (MQ=255)
                                                                       ggCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACACGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGa                                                                        >  2:4748437/1‑149 (MQ=255)
                                                                         cgcTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAAt                                                                      <  1:2333861/149‑1 (MQ=255)
                                                                          gcTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATa                                                                     <  1:708935/149‑1 (MQ=255)
                                                                          gcTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATa                                                                     <  1:299922/149‑1 (MQ=255)
                                                                           cTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAg                                                                    >  2:799468/1‑149 (MQ=255)
                                                                            tGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGc                                                                   >  2:2879556/1‑149 (MQ=255)
                                                                             gTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCg                                                                  >  1:772273/1‑149 (MQ=255)
                                                                               tGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCggg                                                                >  1:4183539/1‑149 (MQ=255)
                                                                                  ggCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGACTAGCGGGCCg                                                             >  2:4372249/1‑149 (MQ=255)
                                                                                     tGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATg                                                          >  1:1071832/1‑149 (MQ=255)
                                                                                         taatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAt                                                      >  1:4649699/1‑149 (MQ=255)
                                                                                          aatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAtt                                                     <  1:3278288/149‑1 (MQ=255)
                                                                                          aatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAtt                                                     <  1:3551628/149‑1 (MQ=255)
                                                                                          aatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAtt                                                     >  1:3890907/1‑149 (MQ=255)
                                                                                                tcATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCTACt                                               >  1:187841/1‑149 (MQ=255)
                                                                                                 cATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTg                                              >  2:72421/1‑149 (MQ=255)
                                                                                                  aTCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTgg                                             <  1:1001215/149‑1 (MQ=255)
                                                                                                    ccAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGAt                                           >  1:2153669/1‑149 (MQ=255)
                                                                                                     cAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATa                                          <  2:2373957/149‑1 (MQ=255)
                                                                                                     cAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATa                                          >  1:588140/1‑149 (MQ=255)
                                                                                                     cAGCGAACTGGTTAGCGCCGTCAATCCGAAACAAGTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATa                                          <  2:711856/149‑1 (MQ=255)
                                                                                                       gCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACGGGATaaa                                        >  2:2557840/1‑149 (MQ=255)
                                                                                                        cGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAg                                       <  2:973781/149‑1 (MQ=255)
                                                                                                          aaCTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGac                                     >  2:110729/1‑149 (MQ=255)
                                                                                                           aCTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGaca                                    >  2:3556422/1‑149 (MQ=255)
                                                                                                           aCTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGaca                                    >  2:696383/1‑149 (MQ=255)
                                                                                                            cTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGacac                                   <  1:2305250/149‑1 (MQ=255)
                                                                                                                ttAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCg                               >  1:893268/1‑149 (MQ=255)
                                                                                                                 tAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCgg                              <  1:3079918/149‑1 (MQ=255)
                                                                                                                   gcgcCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGAt                            <  2:1395330/149‑1 (MQ=255)
                                                                                                                   gcgcCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGAt                            <  2:2232635/149‑1 (MQ=255)
                                                                                                                    cgcCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATg                           <  1:4027790/149‑1 (MQ=255)
                                                                                                                      ccGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCg                         >  1:4383078/1‑149 (MQ=255)
                                                                                                                       cGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCgg                        >  2:1189270/1‑149 (MQ=255)
                                                                                                                        gTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggt                       >  2:4577286/1‑149 (MQ=255)
                                                                                                                           aaTCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGACTAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtggt                    >  2:3612579/1‑149 (MQ=255)
                                                                                                                           aaTCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtggt                    >  1:1607750/1‑149 (MQ=255)
                                                                                                                                gAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAg               <  1:3415004/149‑1 (MQ=255)
                                                                                                                                     aaTTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAat          >  1:4208584/1‑149 (MQ=255)
                                                                                                                                       ttCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAatat        >  1:1157399/1‑149 (MQ=255)
                                                                                                                                        tCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATg       <  2:893268/149‑1 (MQ=255)
                                                                                                                                         cGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGa      <  2:3841101/149‑1 (MQ=255)
                                                                                                                                          gCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGAc     >  2:690130/1‑149 (MQ=255)
                                                                                                                                          gCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGAc     >  2:1583860/1‑149 (MQ=255)
                                                                                                                                            cATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGCCTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTg   >  1:2660111/1‑149 (MQ=255)
                                                                                                                                            cATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTg   >  1:1701679/1‑149 (MQ=255)
                                                                                                                                             aTGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGc  >  1:4260377/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGC  >  CP009273/1439372‑1439661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑