Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,519,349 T→A 100% K297* (AAA→TAA)  ansP ← L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,519,3490TA99.7% 1076.0 / ‑4.7 309K297* (AAA→TAA) ansPL‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (137/170);  total (137/172)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 309 total aligned reads displayed.

TTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGA  >  CP009273/1519204‑1519494
                                                                                                                                                 |                                                                                                                                                 
ttCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAaga                                                                                                                                                <  2:850663/149‑1 (MQ=255)
         aaaCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGt                                                                                                                                       <  2:3089646/149‑1 (MQ=255)
         aaaCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGt                                                                                                                                       >  1:1982579/1‑149 (MQ=255)
         aaaCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGt                                                                                                                                       >  2:1031349/1‑149 (MQ=255)
           aCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCa                                                                                                                                     >  1:2062038/1‑149 (MQ=255)
           aCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCa                                                                                                                                     <  2:1544788/149‑1 (MQ=255)
            cTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCAc                                                                                                                                    <  1:4733260/149‑1 (MQ=255)
            cTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCAc                                                                                                                                    <  1:1546368/149‑1 (MQ=255)
             tCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACg                                                                                                                                   >  2:2769743/1‑149 (MQ=255)
               ggTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGaa                                                                                                                                 >  1:23665/1‑149 (MQ=255)
                gTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAAc                                                                                                                                >  2:1497469/1‑149 (MQ=255)
                 tGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACg                                                                                                                               <  1:4372690/149‑1 (MQ=255)
                   cGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGa                                                                                                                             <  1:2518138/149‑1 (MQ=255)
                    ggAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGAc                                                                                                                            <  2:2427516/149‑1 (MQ=255)
                     gAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACt                                                                                                                           <  2:2487743/149‑1 (MQ=255)
                      aaCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACtt                                                                                                                          >  2:2251008/1‑149 (MQ=255)
                      aaCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACtt                                                                                                                          <  2:3146205/149‑1 (MQ=255)
                       aCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACttt                                                                                                                         >  1:531890/1‑149 (MQ=255)
                       aCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACttt                                                                                                                         <  1:368679/149‑1 (MQ=255)
                       aCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACttt                                                                                                                         >  1:2852732/1‑149 (MQ=255)
                       aCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACttt                                                                                                                         <  1:4670780/149‑1 (MQ=255)
                        ccGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTg                                                                                                                        >  1:4302283/1‑149 (MQ=255)
                           cccATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGccc                                                                                                                     <  1:1429690/149‑1 (MQ=255)
                           cccATCGCCATTGAGCGAAGAATACTTCCGGTGCATTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCAATTTAAGAGAAAAACGTCACGAACGGACTTTGccc                                                                                                                     <  1:2036242/149‑1 (MQ=255)
                            ccATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGcccc                                                                                                                    >  1:199687/1‑149 (MQ=255)
                              aTCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGc                                                                                                                  >  1:1139055/1‑149 (MQ=255)
                              aTCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGc                                                                                                                  >  1:3527490/1‑149 (MQ=255)
                                cGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCt                                                                                                                <  1:1490123/149‑1 (MQ=255)
                                cGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCt                                                                                                                <  1:182399/149‑1 (MQ=255)
                                  ccATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGa                                                                                                              <  1:2572656/149‑1 (MQ=255)
                                  ccATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGa                                                                                                              >  1:1592502/1‑149 (MQ=255)
                                  ccATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGa                                                                                                              <  1:1708878/149‑1 (MQ=255)
                                   cATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGAt                                                                                                             >  1:1102744/1‑149 (MQ=255)
                                     ttGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAc                                                                                                           <  2:79892/149‑1 (MQ=255)
                                       gAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAcgc                                                                                                         <  2:3744421/149‑1 (MQ=255)
                                         gcgcAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACgcgc                                                                                                       <  2:4688208/149‑1 (MQ=255)
                                             aGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCGCGCCTGATACGCGCTCCa                                                                                                   <  2:722500/149‑1 (MQ=255)
                                                aTACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACgg                                                                                                >  2:3371824/1‑149 (MQ=255)
                                                      ccGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataa                                                                                          <  1:1372889/149‑1 (MQ=255)
                                                      ccGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataa                                                                                          <  1:3762249/149‑1 (MQ=255)
                                                       cGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataac                                                                                         >  2:4564059/1‑149 (MQ=255)
                                                        ggTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataaca                                                                                        <  2:4142445/149‑1 (MQ=255)
                                                         gTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacat                                                                                       <  1:841466/149‑1 (MQ=255)
                                                           gCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacataa                                                                                     <  1:4115389/149‑1 (MQ=255)
                                                           gCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacataa                                                                                     <  2:184801/149‑1 (MQ=255)
                                                                 cAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCa                                                                               <  1:2800073/149‑1 (MQ=255)
                                                                  aGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCaa                                                                              <  1:208774/149‑1 (MQ=255)
                                                                  aGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCaa                                                                              >  1:2458540/1‑149 (MQ=255)
                                                                    aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAaca                                                                            >  1:732275/1‑149 (MQ=255)
                                                                    aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAaca                                                                            <  1:3465756/149‑1 (MQ=255)
                                                                     ccTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAacac                                                                           >  1:653307/1‑149 (MQ=255)
                                                                       tGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAAcacca                                                                         >  1:715692/1‑149 (MQ=255)
                                                                       tGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAAcacca                                                                         >  1:416939/1‑149 (MQ=255)
                                                                            tCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAg                                                                    <  1:496242/149‑1 (MQ=255)
                                                                             cAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGc                                                                   <  1:2952508/149‑1 (MQ=255)
                                                                              aGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGcc                                                                  <  1:1427145/149‑1 (MQ=255)
                                                                                  tGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACg                                                              <  2:271045/149‑1 (MQ=255)
                                                                                      gagGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTaaa                                                          >  1:3208394/1‑149 (MQ=255)
                                                                                       agGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTaaaa                                                         >  1:2300960/1‑149 (MQ=255)
                                                                                        gggCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAAc                                                        <  1:4602873/149‑1 (MQ=255)
                                                                                          gCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAg                                                      <  1:2135282/149‑1 (MQ=255)
                                                                                             gCGGTCAGCACCACAATTTTCATATTGCTGCCGATATATGGCACAACCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGcc                                                   <  1:1641534/149‑1 (MQ=255)
                                                                                               ggTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCaa                                                 <  2:2893651/149‑1 (MQ=255)
                                                                                                 tCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACCCCACGGAGCCGACGTAAAACAGGCCAATa                                               <  1:3137232/149‑1 (MQ=255)
                                                                                                 tCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATa                                               <  1:3083568/149‑1 (MQ=255)
                                                                                                 tCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCCCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATa                                               >  1:3834897/1‑149 (MQ=255)
                                                                                                  cAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATAc                                              <  1:4384335/149‑1 (MQ=255)
                                                                                                      accacAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCa                                          >  1:3826042/1‑149 (MQ=255)
                                                                                                        cacaATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCACTAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaaa                                        >  2:3517726/1‑149 (MQ=255)
                                                                                                        cacaATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaaa                                        >  2:134692/1‑149 (MQ=255)
                                                                                                         acaATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAAt                                       >  2:253568/1‑149 (MQ=255)
                                                                                                            aTGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcac                                    >  1:3220663/1‑149 (MQ=255)
                                                                                                            aTGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcac                                    >  1:1016170/1‑149 (MQ=255)
                                                                                                            aTGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcac                                    >  2:4059721/1‑149 (MQ=255)
                                                                                                             tGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcaca                                   <  2:1594458/149‑1 (MQ=255)
                                                                                                              gTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCacac                                  >  1:3961957/1‑149 (MQ=255)
                                                                                                               ttCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                 >  2:1902087/1‑149 (MQ=255)
                                                                                                               ttCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                 <  1:3974161/149‑1 (MQ=255)
                                                                                                                 cATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGt                               <  1:360965/149‑1 (MQ=255)
                                                                                                                  aTAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGtt                              <  2:4119051/149‑1 (MQ=255)
                                                                                                                   tAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTa                             >  2:1670778/1‑149 (MQ=255)
                                                                                                                     aTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAAt                           >  1:3904390/1‑149 (MQ=255)
                                                                                                                     aTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAAt                           >  2:1249571/1‑149 (MQ=255)
                                                                                                                     aTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAAt                           >  1:34051/1‑149 (MQ=255)
                                                                                                                     aTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAAt                           >  2:3639953/1‑149 (MQ=255)
                                                                                                                      tgctgcCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATg                          <  2:233320/149‑1 (MQ=255)
                                                                                                                         tgcCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCt                       >  2:2054736/1‑149 (MQ=255)
                                                                                                                             gATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAg                   <  2:4357346/149‑1 (MQ=255)
                                                                                                                             gATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAg                   <  2:2959302/149‑1 (MQ=255)
                                                                                                                              atatatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAgt                  >  2:3465076/1‑148 (MQ=255)
                                                                                                                               tatatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc                 >  1:1254548/1‑149 (MQ=255)
                                                                                                                                atatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCa                <  2:3544613/149‑1 (MQ=255)
                                                                                                                                atatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCa                >  2:1020902/1‑149 (MQ=255)
                                                                                                                                atatGGCACACCCAGTTAAGACAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAACCAGGCCAATACGCCAAATCACACTGTTAAGGGCTTGAGGCa                <  2:3617731/149‑1 (MQ=255)
                                                                                                                                   tGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCa             <  1:2994349/149‑1 (MQ=255)
                                                                                                                                    ggCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCAt            >  2:551608/1‑149 (MQ=255)
                                                                                                                                     gCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATg           <  1:2231263/149‑1 (MQ=255)
                                                                                                                                       acacCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGt         >  2:4222979/1‑149 (MQ=255)
                                                                                                                                        cacCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTc        >  2:1800437/1‑149 (MQ=255)
                                                                                                                                         acCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCt       >  1:577786/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGA  >  CP009273/1519204‑1519494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑