BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	126,784	C→A	6.0%	D322Y (GAT→TAT)	yacH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	126,784	0	C	A	6.0%	1076.7 / 27.6	282	D322Y (GAT→TAT)	yacH	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (156/109); new base A (7/10); total (163/119)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

Only 100 of 289 total aligned reads displayed.

GGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCT  >  CP009273/126636‑126932

ggCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATATGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATc                                                                                                                                                      >  2:4212812/1‑149 (MQ=255)

ggCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATc                                                                                                                                                      <  2:597613/149‑1 (MQ=255)

 gCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCg                                                                                                                                                     >  2:4300584/1‑149 (MQ=255)

   acacCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgtg                                                                                                                                                   <  2:775990/149‑1 (MQ=255)

   acacCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgtg                                                                                                                                                   <  2:4575072/149‑1 (MQ=255)

   acacCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgtg                                                                                                                                                   <  2:1291366/149‑1 (MQ=255)

     acCATTACGGAAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgtggt                                                                                                                                                 <  1:1592516/149‑1 (MQ=255)

       cATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGtga                                                                                                                                               <  1:603724/149‑1 (MQ=255)

        aTTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtgat                                                                                                                                              >  2:2337056/1‑149 (MQ=255)

         ttACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtgatg                                                                                                                                             >  2:765217/1‑149 (MQ=255)

         ttACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtgatg                                                                                                                                             <  1:708756/149‑1 (MQ=255)

         ttACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtgatg                                                                                                                                             >  2:1703700/1‑149 (MQ=255)

          tACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtgatga                                                                                                                                            <  1:1679722/149‑1 (MQ=255)

           aCGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTgatgat                                                                                                                                           <  2:3631571/149‑1 (MQ=255)

            cGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATa                                                                                                                                          <  1:2169716/149‑1 (MQ=255)

              gTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAat                                                                                                                                        >  1:3079562/1‑149 (MQ=255)

                aGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAatca                                                                                                                                      >  1:1887422/1‑149 (MQ=255)

                 gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAatcat                                                                                                                                     >  1:2308017/1‑149 (MQ=255)

                 gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAatcat                                                                                                                                     >  1:397308/1‑149 (MQ=255)

                    tGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATcatcat                                                                                                                                  >  1:1355773/1‑149 (MQ=255)

                     ggATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCAtt                                                                                                                                 <  1:1287817/149‑1 (MQ=255)

                      gATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTg                                                                                                                                <  1:3507140/149‑1 (MQ=255)

                      gATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTg                                                                                                                                <  1:4004542/149‑1 (MQ=255)

                       aTTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGt                                                                                                                               <  2:2028195/149‑1 (MQ=255)

                       aTTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGt                                                                                                                               >  2:3428868/1‑149 (MQ=255)

                            gccgccATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatga                                                                                                                          <  1:4430621/149‑1 (MQ=255)

                              cgccATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatg                                                                                                                        <  1:794501/149‑1 (MQ=255)

                                ccatgccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgat                                                                                                                      <  2:3493425/149‑1 (MQ=255)

                                   tgccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatgat                                                                                                                   >  1:1745857/1‑149 (MQ=255)

                                    gccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatgatg                                                                                                                  >  2:3725949/1‑149 (MQ=255)

                                        tatTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCa                                                                                                              <  1:632719/149‑1 (MQ=255)

                                          ttCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATg                                                                                                            >  1:924912/1‑149 (MQ=255)

                                          ttCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATg                                                                                                            >  1:2540701/1‑149 (MQ=255)

                                           tCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGa                                                                                                           <  2:2643624/149‑1 (MQ=255)

                                            cTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAt                                                                                                          <  2:3321087/149‑1 (MQ=255)

                                             ttATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtc                                                                                                         >  1:935029/1‑149 (MQ=255)

                                             ttATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtc                                                                                                         >  2:2549229/1‑149 (MQ=255)

                                               aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgt                                                                                                       <  2:552582/149‑1 (MQ=255)

                                               aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGGTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgt                                                                                                       <  2:3201686/149‑1 (MQ=255)

                                                tCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtc                                                                                                      >  2:4787877/1‑149 (MQ=255)

                                                 cAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcg                                                                                                     >  2:2257585/1‑149 (MQ=255)

                                                  aGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgt                                                                                                    <  1:3725949/149‑1 (MQ=255)

                                                    tAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATTTTTTCGCCTTTGTGTTGCCAGCCATTAACGTCAAGATGACCGACATCTTGGTGATGATAATCATCATTGTCATGATGATGATTTTCATGAtcgtcgtcg                                                                                                  >  1:4160727/1‑149 (MQ=255)

                                                    tAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgtcg                                                                                                  >  2:1857116/1‑149 (MQ=255)

                                                    tAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgtcg                                                                                                  >  2:4246844/1‑149 (MQ=255)

                                                       gATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTcc                                                                                               >  1:1067626/1‑149 (MQ=255)

                                                       gATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTcc                                                                                               >  2:4304914/1‑149 (MQ=255)

                                                        aTGCTCACCGTTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTccc                                                                                              >  1:1063572/1‑149 (MQ=255)

                                                        aTGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTccc                                                                                              <  1:3484635/149‑1 (MQ=255)

                                                         tGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCa                                                                                             >  1:3421485/1‑149 (MQ=255)

                                                            tCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTc                                                                                          <  1:2611228/149‑1 (MQ=255)

                                                              aCCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGa                                                                                        <  2:3929278/149‑1 (MQ=255)

                                                                cGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGAtg                                                                                      <  1:1793018/149‑1 (MQ=255)

                                                                cGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGAtg                                                                                      >  2:1387336/1‑149 (MQ=255)

                                                                  gTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGAtgct                                                                                    <  2:3806505/149‑1 (MQ=255)

                                                                    gATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGAtgctgc                                                                                  >  2:1063209/1‑149 (MQ=255)

                                                                     aTACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATgctgct                                                                                 >  2:4095742/1‑149 (MQ=255)

                                                                     aTACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATgctgct                                                                                 >  2:1305941/1‑149 (MQ=255)

                                                                      tACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGctgctg                                                                                >  1:2964262/1‑149 (MQ=255)

                                                                      tACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGctgctg                                                                                >  1:572348/1‑149 (MQ=255)

                                                                      tACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATCATGATGATGGTCATGATCGTCGTCGTCCCAGTCGAAGatgctg                                                                                >  2:4457212/1‑149 (MQ=255)

                                                                        cGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGaa                                                                              <  1:3917735/149‑1 (MQ=255)

                                                                         ggTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAAt                                                                             >  1:3437364/1‑149 (MQ=255)

                                                                           ttGAACGTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTGGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAg                                                                           <  2:3995279/149‑1 (MQ=255)

                                                                            tGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGGTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGt                                                                          <  1:4534142/149‑1 (MQ=255)

                                                                             gAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTg                                                                         >  2:3002539/1‑149 (MQ=255)

                                                                             gAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTg                                                                         <  1:3679461/149‑1 (MQ=255)

                                                                             gAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTg                                                                         >  1:3250377/1‑149 (MQ=255)

                                                                             gAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTg                                                                         >  1:4681837/1‑149 (MQ=255)

                                                                             gAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTg                                                                         <  2:1118649/149‑1 (MQ=255)

                                                                              aaaTTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGc                                                                        >  2:2837565/1‑149 (MQ=255)

                                                                                 ttgttgACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTa                                                                     >  1:2666569/1‑149 (MQ=255)

                                                                                    ttgACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCTATGCTGCTGAATAGTGCGTACGt                                                                  >  2:2105340/1‑149 (MQ=255)

                                                                                      gACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTgg                                                                >  2:240180/1‑149 (MQ=255)

                                                                                        cGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTa                                                              >  2:1163999/1‑149 (MQ=255)

                                                                                         gTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAg                                                             >  1:3824565/1‑149 (MQ=255)

                                                                                              tatTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACg                                                        >  2:3363017/1‑149 (MQ=255)

                                                                                              tatTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACg                                                        >  1:3020686/1‑149 (MQ=255)

                                                                                              tatTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTAAGTGGTAGCAAct                                                        >  1:2453116/1‑148 (MQ=255)

                                                                                                 tGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGccc                                                     <  1:1219182/149‑1 (MQ=255)

                                                                                                      ttgtCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCt                                                >  1:210987/1‑149 (MQ=255)

                                                                                                         tCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCtata                                             >  1:4523459/1‑149 (MQ=255)

                                                                                                            ccGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCCTGCTATAGcc                                          >  2:1644891/1‑149 (MQ=255)

                                                                                                              gTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGa                                        >  2:4701838/1‑149 (MQ=255)

                                                                                                               ttGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGaa                                       <  1:3628211/149‑1 (MQ=255)

                                                                                                                 gtgtTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATc                                     <  2:455518/149‑1 (MQ=255)

                                                                                                                    ttGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCcgc                                  <  1:1947669/149‑1 (MQ=255)

                                                                                                                      gccagccaTTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGt                                >  1:2984043/1‑149 (MQ=255)

                                                                                                                        cagccaTTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTAc                              <  2:4056839/149‑1 (MQ=255)

                                                                                                                             aTTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGc                         >  1:872477/1‑149 (MQ=255)

                                                                                                                              ttACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCt                        <  2:101782/149‑1 (MQ=255)

                                                                                                                              ttACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCt                        <  1:2761324/149‑1 (MQ=255)

                                                                                                                                    tCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGCAGCCGCGTCCCAAGCTGTcaac                  >  2:2472731/1‑149 (MQ=255)

                                                                                                                                       cGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACaaa               >  2:4285166/1‑149 (MQ=255)

                                                                                                                                        gATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAAc              >  1:1239483/1‑149 (MQ=255)

                                                                                                                                          tGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACgg            >  2:3570368/1‑149 (MQ=255)

                                                                                                                                           gACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGt           <  1:4394332/149‑1 (MQ=255)

                                                                                                                                            aCCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGtt          >  1:498611/1‑149 (MQ=255)

                                                                                                                                            aCCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGtt          <  1:1134699/149‑1 (MQ=255)

                                                                                                                                                 cATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCg     >  2:3949219/1‑149 (MQ=255)

GGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCT  >  CP009273/126636‑126932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`