BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,610,766	C→T	8.4%	S249S (AGC→AGT)	yneK →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,610,766	0	C	T	8.4%	934.3 / 49.5	274	S249S (AGC→AGT)	yneK	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (103/147); new base T (4/19); total (108/166)
Fisher's exact test for biased strand distribution p-value = 2.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 278 total aligned reads displayed.

TTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGA  >  CP009273/1610619‑1610914

ttttATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGcc                                                                                                                                                     <  2:528232/149‑1 (MQ=255)

 tttATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTTACTTGATCACCTCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCt                                                                                                                                                    <  1:848489/149‑1 (MQ=255)

 tttATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCt                                                                                                                                                    <  1:2717945/149‑1 (MQ=255)

    aTCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGAt                                                                                                                                                 <  1:1228383/149‑1 (MQ=255)

    aTCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGAt                                                                                                                                                 <  1:3168222/149‑1 (MQ=255)

     tctctcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATg                                                                                                                                                <  1:1915143/149‑1 (MQ=255)

       tctcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGa                                                                                                                                              <  2:2520218/149‑1 (MQ=255)

        ctcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGAt                                                                                                                                             <  1:4287250/149‑1 (MQ=255)

           gcgGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCa                                                                                                                                          >  1:877294/1‑149 (MQ=255)

           gcgGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCa                                                                                                                                          <  2:2166404/149‑1 (MQ=255)

           gcgGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCa                                                                                                                                          <  2:351846/149‑1 (MQ=255)

             ggTAATATTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCAtt                                                                                                                                        <  2:1317956/149‑1 (MQ=255)

              gTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTa                                                                                                                                       >  1:1553206/1‑149 (MQ=255)

              gTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTa                                                                                                                                       <  1:4225412/149‑1 (MQ=255)

              gTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTa                                                                                                                                       >  1:3943433/1‑149 (MQ=255)

                      gTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTaa                                                                                                                               >  1:475028/1‑149 (MQ=255)

                           aCTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTaacaaac                                                                                                                          >  1:1177218/1‑149 (MQ=255)

                            cTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTaacaaaca                                                                                                                         >  1:1971422/1‑149 (MQ=255)

                            cTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTaacaaaca                                                                                                                         <  2:2926437/149‑1 (MQ=255)

                             tCCCCCTATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACAt                                                                                                                        >  1:957274/1‑149 (MQ=255)

                              gccccGTTTATGGAATATCTCTGTGGTTCACAGATGGTTCGTCAATATAGATCCTTACTTGATCACCGCCATTCTCGTCATTACGGACAGGGAATTAATAGATGATTTGTTGCTAAGCCTGATGGATTCATTAGGTTCTAACTATCATg                                                                                                                       <  2:4431580/148‑1 (MQ=255)

                                cccGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGta                                                                                                                     <  1:1962831/149‑1 (MQ=255)

                                cccGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGta                                                                                                                     >  2:650915/1‑149 (MQ=255)

                                 ccGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGtac                                                                                                                    <  2:46421/149‑1 (MQ=255)

                                    aTTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGtactac                                                                                                                 >  2:4487132/1‑149 (MQ=255)

                                    aTTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGtactac                                                                                                                 >  2:3753263/1‑149 (MQ=255)

                                      tATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAccc                                                                                                               <  1:2230487/149‑1 (MQ=255)

                                                ctctGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCa                                                                                                     >  1:3366699/1‑149 (MQ=255)

                                                  ctGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATc                                                                                                   <  2:3639169/149‑1 (MQ=255)

                                                    gAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTg                                                                                                 <  1:3017615/149‑1 (MQ=255)

                                                     aGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGc                                                                                                <  2:71569/149‑1 (MQ=255)

                                                      ggTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCt                                                                                               <  2:3303197/149‑1 (MQ=255)

                                                      ggTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCt                                                                                               <  2:2343232/149‑1 (MQ=255)

                                                       gTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCtt                                                                                              <  2:1411588/149‑1 (MQ=255)

                                                       gTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCtt                                                                                              >  1:74085/1‑149 (MQ=255)

                                                        ttCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCttt                                                                                             >  2:4774716/1‑149 (MQ=255)

                                                        ttCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCttt                                                                                             <  2:4571458/149‑1 (MQ=255)

                                                         tCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCtttt                                                                                            <  1:3630440/149‑1 (MQ=255)

                                                          cacaGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCttttt                                                                                           <  2:3837134/149‑1 (MQ=255)

                                                          cacaGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCttttt                                                                                           >  2:3108436/1‑149 (MQ=255)

                                                              gATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATcc                                                                                       <  1:2004789/149‑1 (MQ=255)

                                                              gATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATcc                                                                                       >  1:2254752/1‑149 (MQ=255)

                                                              gATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATcc                                                                                       >  2:2822894/1‑149 (MQ=255)

                                                               aTGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATccc                                                                                      <  1:1022688/149‑1 (MQ=255)

                                                                  gTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCttt                                                                                   <  2:1592848/149‑1 (MQ=255)

                                                                    tCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTa                                                                                 <  1:4574760/149‑1 (MQ=255)

                                                                      gTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGc                                                                               <  1:4000350/149‑1 (MQ=255)

                                                                             aaGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACaa                                                                        >  2:2034174/1‑149 (MQ=255)

                                                                                 tCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGACCAAGAAt                                                                    >  2:3268799/1‑149 (MQ=255)

                                                                                   cTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAAtct                                                                  <  1:2436001/149‑1 (MQ=255)

                                                                                    tAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAAtctc                                                                 >  1:3976014/1‑149 (MQ=255)

                                                                                      aCTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcct                                                               >  2:871086/1‑149 (MQ=255)

                                                                                      aCTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcct                                                               <  1:998061/149‑1 (MQ=255)

                                                                                      aCTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcct                                                               >  1:1716217/1‑149 (MQ=255)

                                                                                        ttGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCa                                                             >  2:4075591/1‑149 (MQ=255)

                                                                                        ttGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCa                                                             >  1:692526/1‑149 (MQ=255)

                                                                                         tGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAt                                                            >  2:1491112/1‑149 (MQ=255)

                                                                                          gATCACCGCCATTCACGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAtt                                                           <  2:2402505/149‑1 (MQ=255)

                                                                                            tCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAtttt                                                         >  1:1073760/1‑149 (MQ=255)

                                                                                            tCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAtttt                                                         <  2:3653342/149‑1 (MQ=255)

                                                                                             cACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGTATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTc                                                        >  1:1627618/1‑149 (MQ=255)

                                                                                               ccGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAg                                                      <  1:1780639/149‑1 (MQ=255)

                                                                                                cGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGa                                                     >  2:173926/1‑149 (MQ=255)

                                                                                                cGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGa                                                     <  2:3036131/149‑1 (MQ=255)

                                                                                                 gCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGaa                                                    >  1:2546796/1‑149 (MQ=255)

                                                                                                  ccATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATTGTTTCATTAGGTTCTATCAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAAc                                                   <  2:1196299/149‑1 (MQ=255)

                                                                                                   cATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACt                                                  >  1:3798876/1‑149 (MQ=255)

                                                                                                    aTTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACtt                                                 <  1:3386825/149‑1 (MQ=255)

                                                                                                    aTTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACtt                                                 >  1:1346967/1‑149 (MQ=255)

                                                                                                    aTTCTCGAAATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACtt                                                 <  2:2568516/149‑1 (MQ=255)

                                                                                                     ttCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTg                                                >  1:1835685/1‑149 (MQ=255)

                                                                                                      tctcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGt                                               <  1:3454404/149‑1 (MQ=255)

                                                                                                      tctcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGt                                               <  1:4255055/149‑1 (MQ=255)

                                                                                                        tcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttt                                             >  2:2317680/1‑149 (MQ=255)

                                                                                                        tcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttt                                             >  1:2882203/1‑149 (MQ=255)

                                                                                                          gACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttttt                                           <  2:2889046/149‑1 (MQ=255)

                                                                                                           aCATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTa                                          >  2:2734364/1‑149 (MQ=255)

                                                                                                            cATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAt                                         >  2:4528122/1‑149 (MQ=255)

                                                                                                             aTAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAtt                                        >  1:2128306/1‑149 (MQ=255)

                                                                                                              tAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttt                                       <  2:4182411/149‑1 (MQ=255)

                                                                                                                aCGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttttt                                     <  2:2864805/149‑1 (MQ=255)

                                                                                                                   gACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGc                                  >  2:3150555/1‑149 (MQ=255)

                                                                                                                    aCAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGcc                                 >  1:4550740/1‑149 (MQ=255)

                                                                                                                     cAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCa                                >  1:2386960/1‑149 (MQ=255)

                                                                                                                       gCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAat                              <  2:3355792/149‑1 (MQ=255)

                                                                                                                        cGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAata                             <  1:1304541/149‑1 (MQ=255)

                                                                                                                        cGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAata                             <  2:2961778/149‑1 (MQ=255)

                                                                                                                         gAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAatat                            >  2:1384996/1‑149 (MQ=255)

                                                                                                                           aTTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGt                          <  1:3548419/149‑1 (MQ=255)

                                                                                                                            ttAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGtt                         <  1:4109570/149‑1 (MQ=255)

                                                                                                                             tAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTa                        <  1:3233866/149‑1 (MQ=255)

                                                                                                                                aaGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTg                     <  2:3866222/149‑1 (MQ=255)

                                                                                                                                 agagGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGc                    <  1:4230893/149‑1 (MQ=255)

                                                                                                                                 agagGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCACTATGTTATTGc                    >  2:4092388/1‑149 (MQ=255)

                                                                                                                                 agagGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGc                    <  2:1796546/149‑1 (MQ=255)

                                                                                                                                    ggATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACt                 >  2:4124238/1‑149 (MQ=255)

                                                                                                                                       tttGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATc              >  1:560970/1‑149 (MQ=255)

                                                                                                                                            tgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCaa         <  1:1505274/149‑1 (MQ=255)

                                                                                                                                               aaaGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAttt      >  2:1923528/1‑149 (MQ=255)

                                                                                                                                               aaaGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAttt      >  2:1127060/1‑149 (MQ=255)

TTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGA  >  CP009273/1610619‑1610914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`