Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 137,071 G→T 88.7% I214I (ATC→ATA)  gcd ← glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273137,0710GT88.7% 878.1 / 111.9 336I214I (ATC→ATA) gcdglucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (15/23);  new base T (137/161);  total (152/184)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 336 total aligned reads displayed.

TACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCT  >  CP009273/136924‑137216
                                                                                                                                                   |                                                                                                                                                 
tACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGa                                                                                                                                                  <  1:4509101/149‑1 (MQ=255)
 aCGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCAGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAt                                                                                                                                                 >  1:925016/1‑149 (MQ=255)
  cGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAtt                                                                                                                                                <  1:2029634/149‑1 (MQ=255)
  cGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCATCACTTCATTGGTTAtt                                                                                                                                                <  2:1255929/149‑1 (MQ=255)
   gTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAttt                                                                                                                                               <  2:4681384/149‑1 (MQ=255)
      cTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCAc                                                                                                                                            <  2:721862/149‑1 (MQ=255)
       tGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCAcc                                                                                                                                           <  1:744291/149‑1 (MQ=255)
        ggAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCAccc                                                                                                                                          <  2:4063339/149‑1 (MQ=255)
        ggAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATGTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCAccc                                                                                                                                          <  2:1777988/149‑1 (MQ=255)
            aGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGAt                                                                                                                                      <  2:612668/149‑1 (MQ=255)
               cTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGt                                                                                                                                   <  1:1882531/149‑1 (MQ=255)
                tCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGtt                                                                                                                                  >  1:2932173/1‑149 (MQ=255)
                tCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGtt                                                                                                                                  <  2:597504/149‑1 (MQ=255)
                     ggTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgct                                                                                                                             <  2:2390999/149‑1 (MQ=255)
                     ggTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGgct                                                                                                                             <  2:4457333/149‑1 (MQ=255)
                       ttttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgctgc                                                                                                                           >  1:3428387/1‑149 (MQ=255)
                         ttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCtt                                                                                                                         >  2:2960193/1‑149 (MQ=255)
                            aGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTcac                                                                                                                      >  2:4173568/1‑149 (MQ=255)
                             gCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTcaca                                                                                                                     >  2:1786878/1‑149 (MQ=255)
                             gCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTcaca                                                                                                                     >  2:2660965/1‑149 (MQ=255)
                             gCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTcaca                                                                                                                     >  2:3452679/1‑149 (MQ=255)
                                   gATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCa                                                                                                               <  1:2503187/149‑1 (MQ=255)
                                   gATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCa                                                                                                               <  2:3363357/149‑1 (MQ=255)
                                        tAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACg                                                                                                          <  1:713330/149‑1 (MQ=255)
                                           tGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGaa                                                                                                       <  2:120037/149‑1 (MQ=255)
                                            gCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAac                                                                                                      <  1:654831/149‑1 (MQ=255)
                                             ccATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAaca                                                                                                     >  2:3492303/1‑149 (MQ=255)
                                             ccATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCAGTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAaca                                                                                                     >  2:777107/1‑149 (MQ=255)
                                               aTTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                   >  2:3146595/1‑149 (MQ=255)
                                                 ttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCa                                                                                                 >  2:2315923/1‑149 (MQ=255)
                                                 ttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCa                                                                                                 <  1:3116384/149‑1 (MQ=255)
                                                  tctcTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAg                                                                                                <  1:297167/149‑1 (MQ=255)
                                                     ctTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGct                                                                                             <  1:3740385/149‑1 (MQ=255)
                                                       ttGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttc                                                                                           <  2:1578261/149‑1 (MQ=255)
                                                       ttGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttc                                                                                           <  2:3256916/149‑1 (MQ=255)
                                                          ccGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCttt                                                                                        <  1:1543583/149‑1 (MQ=255)
                                                          ccGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCttt                                                                                        <  1:198904/149‑1 (MQ=255)
                                                              tGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGa                                                                                    >  2:144538/1‑149 (MQ=255)
                                                              tGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGa                                                                                    >  1:2684532/1‑149 (MQ=255)
                                                                gcggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGAtt                                                                                  <  2:1713281/149‑1 (MQ=255)
                                                                 cggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTa                                                                                 <  1:3273607/149‑1 (MQ=255)
                                                                   gcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATg                                                                               >  1:3092631/1‑149 (MQ=255)
                                                                   gcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATg                                                                               <  1:1678990/149‑1 (MQ=255)
                                                                     aTCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGa                                                                             <  2:1869606/149‑1 (MQ=255)
                                                                      tCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGAc                                                                            >  1:4678177/1‑149 (MQ=255)
                                                                       cAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACg                                                                           >  2:1048611/1‑149 (MQ=255)
                                                                        aaGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTTGTTATTTCACCCGGATCGTTCGGCTGCTTCTCATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGt                                                                          <  2:2995063/149‑1 (MQ=255)
                                                                        aaGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGt                                                                          <  1:3648999/149‑1 (MQ=255)
                                                                        aaGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGt                                                                          <  2:38162/149‑1 (MQ=255)
                                                                         aGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGtt                                                                         <  1:4790236/149‑1 (MQ=255)
                                                                         aGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGtt                                                                         <  1:2308438/149‑1 (MQ=255)
                                                                           cgcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTAt                                                                       <  1:3387098/149‑1 (MQ=255)
                                                                            gcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATc                                                                      <  2:223423/149‑1 (MQ=255)
                                                                             cAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCg                                                                     >  1:2155367/1‑149 (MQ=255)
                                                                               aaCAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGc                                                                   <  2:3224797/149‑1 (MQ=255)
                                                                               aaCAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGc                                                                   <  2:4324826/149‑1 (MQ=255)
                                                                               aaCAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGc                                                                   >  2:1920902/1‑149 (MQ=255)
                                                                                  aGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGtt                                                                <  1:4251065/149‑1 (MQ=255)
                                                                                         ggTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGt                                                         >  1:2335052/1‑149 (MQ=255)
                                                                                         ggTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTGTATTTGt                                                         >  1:1312310/1‑149 (MQ=255)
                                                                                           tGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGttt                                                       <  1:207510/149‑1 (MQ=255)
                                                                                            gAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTc                                                      <  1:2805943/149‑1 (MQ=255)
                                                                                            gAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTc                                                      <  2:251848/149‑1 (MQ=255)
                                                                                              gCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAg                                                    <  2:921823/149‑1 (MQ=255)
                                                                                                 gTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAgcgg                                                 <  1:4730296/149‑1 (MQ=255)
                                                                                                  tACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAgcggc                                                <  1:883775/149‑1 (MQ=255)
                                                                                                   acacAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAgcggcg                                               <  1:4348804/149‑1 (MQ=255)
                                                                                                    cacaGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGa                                              <  1:3211271/149‑1 (MQ=255)
                                                                                                      caGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGaaa                                            <  2:4711065/149‑1 (MQ=255)
                                                                                                       aGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTAAGCGGCGaaaa                                           >  1:4061369/1‑149 (MQ=255)
                                                                                                          tAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCg                                        >  1:3991406/1‑149 (MQ=255)
                                                                                                                gtgtCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAcc                                  <  1:3923295/149‑1 (MQ=255)
                                                                                                                 tgtCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCt                                 <  2:2139094/149‑1 (MQ=255)
                                                                                                                    cGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTcc                              <  1:3601144/149‑1 (MQ=255)
                                                                                                                    cGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTcc                              <  2:4523321/149‑1 (MQ=255)
                                                                                                                     gCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCATGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCt                             <  2:2183684/149‑1 (MQ=255)
                                                                                                                      cccACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTg                            >  1:3169562/1‑149 (MQ=255)
                                                                                                                       ccACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGa                           >  1:1179736/1‑149 (MQ=255)
                                                                                                                        cACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGAt                          <  1:1976162/149‑1 (MQ=255)
                                                                                                                          cTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTa                        <  1:1396175/149‑1 (MQ=255)
                                                                                                                          cTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTa                        <  1:1853163/149‑1 (MQ=255)
                                                                                                                           tttAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAAATTGTTTCAGCGGCGAAAAGCGATGACCTTCCTGATTAc                       >  1:4127209/1‑149 (MQ=255)
                                                                                                                             tAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGa                     >  2:1166952/1‑149 (MQ=255)
                                                                                                                              aaTCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGAc                    <  2:3641134/149‑1 (MQ=255)
                                                                                                                                  ggCGTCTCTTCTTTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATa                <  1:2621368/149‑1 (MQ=255)
                                                                                                                                   gCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACATCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCATCCTGATTACGACCATag               >  1:4274717/1‑149 (MQ=255)
                                                                                                                                   gCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATag               >  1:3019019/1‑149 (MQ=255)
                                                                                                                                   gCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTAAGCGGCGAAAAGCGTTGACCTTCCTGATTAAGAACATag               >  1:2241210/1‑149 (MQ=255)
                                                                                                                                     gTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggc             >  2:4391257/1‑149 (MQ=255)
                                                                                                                                      tCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggca            <  1:4585535/149‑1 (MQ=255)
                                                                                                                                         cTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggcaggc         <  1:2588879/149‑1 (MQ=255)
                                                                                                                                         cTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggcaggc         >  2:479606/1‑149 (MQ=255)
                                                                                                                                          ttCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTTTTTAAGCGGCGAAAAGCGTTGACCATCCTGATTACCACCATAGGCAGGcc        >  1:1731108/1‑149 (MQ=255)
                                                                                                                                          ttCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGcc        <  1:3526993/149‑1 (MQ=255)
                                                                                                                                          ttCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGcc        <  1:4184828/149‑1 (MQ=255)
                                                                                                                                          ttCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGAGGCGAAAAGCGTTTACCTTCCTGATTAAGACCAAATGCAGGcc        >  1:1683203/1‑149 (MQ=255)
                                                                                                                                            cATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAg      <  1:1564917/149‑1 (MQ=255)
                                                                                                                                             aTTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGt     >  1:306470/1‑149 (MQ=255)
                                                                                                                                               tGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTcc   <  1:1002279/149‑1 (MQ=255)
                                                                                                                                                ggTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTTATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCt  >  1:1906342/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCT  >  CP009273/136924‑137216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑