Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,696,612 A→T 100% E41D (GAA→GAT)  add → adenosine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,696,6120AT99.6% 952.2 / ‑4.6 276E41D (GAA→GAT) addadenosine deaminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (153/122);  minor base C (0/1);  total (153/123)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 276 total aligned reads displayed.

TATATCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGT  >  CP009273/1696466‑1696756
                                                                                                                                                  |                                                                                                                                                
tataTCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATac                                                                                                                                                >  1:2999891/1‑149 (MQ=255)
   atCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACt                                                                                                                                             >  1:963694/1‑149 (MQ=255)
    tCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTg                                                                                                                                            >  1:290052/1‑149 (MQ=255)
     ccAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGa                                                                                                                                           <  1:3819119/149‑1 (MQ=255)
        gAACAAGAGAGCGACCATGATTGATACCACCCTGCCAGTAACTGAGATCCCTCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTc                                                                                                                                        <  1:1256975/149‑1 (MQ=255)
        gAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTc                                                                                                                                        <  1:598528/149‑1 (MQ=255)
          aaaaGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTccc                                                                                                                                      >  1:3569877/1‑149 (MQ=255)
          aaaaGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTccc                                                                                                                                      >  1:4606465/1‑149 (MQ=255)
          aaaaGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGAGTccc                                                                                                                                      <  1:3932731/149‑1 (MQ=255)
           aaaGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTcccc                                                                                                                                     <  2:2447795/149‑1 (MQ=255)
            aaGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCa                                                                                                                                    >  2:69576/1‑149 (MQ=255)
             agagTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCAc                                                                                                                                   >  1:4044730/1‑149 (MQ=255)
             agagTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCAc                                                                                                                                   >  1:3708047/1‑149 (MQ=255)
              gagTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACg                                                                                                                                  >  1:2121741/1‑149 (MQ=255)
                gTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGtt                                                                                                                                >  2:3428058/1‑149 (MQ=255)
                gTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGtt                                                                                                                                >  2:457949/1‑149 (MQ=255)
                   cGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAg                                                                                                                             <  1:3886318/149‑1 (MQ=255)
                     aCCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGt                                                                                                                           <  2:782956/149‑1 (MQ=255)
                       cATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCa                                                                                                                         >  1:4637256/1‑149 (MQ=255)
                       cATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCa                                                                                                                         >  2:4158764/1‑149 (MQ=255)
                         tgattgatACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCAtt                                                                                                                       >  2:3600347/1‑149 (MQ=255)
                          gattgatACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTg                                                                                                                      >  2:2262012/1‑149 (MQ=255)
                          gattgatACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTg                                                                                                                      >  1:1418806/1‑149 (MQ=255)
                            ttgatACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGcc                                                                                                                    <  2:1083072/149‑1 (MQ=255)
                                 accaccCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGa                                                                                                               >  2:1566984/1‑149 (MQ=255)
                                 accaccCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGa                                                                                                               <  2:2014956/149‑1 (MQ=255)
                                  ccaccCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGaa                                                                                                              >  1:555040/1‑149 (MQ=255)
                                  ccaccCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGaa                                                                                                              >  2:1443093/1‑149 (MQ=255)
                                  ccaccCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGaa                                                                                                              >  1:1714826/1‑149 (MQ=255)
                                    accCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAAcc                                                                                                            >  1:2502817/1‑149 (MQ=255)
                                     cccTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAAccc                                                                                                           <  1:1087143/149‑1 (MQ=255)
                                     cccTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAAccc                                                                                                           <  1:1662541/149‑1 (MQ=255)
                                        tGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGAt                                                                                                        <  2:4781132/149‑1 (MQ=255)
                                        tGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGAt                                                                                                        <  1:4564656/149‑1 (MQ=255)
                                          ccATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCt                                                                                                      >  1:3076049/1‑149 (MQ=255)
                                           cATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTg                                                                                                     <  2:287227/149‑1 (MQ=255)
                                            aTTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTgg                                                                                                    <  1:4343934/149‑1 (MQ=255)
                                             ttAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGt                                                                                                   >  2:2837989/1‑149 (MQ=255)
                                               aaCTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGa                                                                                                 <  1:1678651/149‑1 (MQ=255)
                                                 cTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGc                                                                                               <  2:3156101/149‑1 (MQ=255)
                                                 cTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGc                                                                                               <  2:2116755/149‑1 (MQ=255)
                                                     tatCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTc                                                                                           >  2:3971247/1‑149 (MQ=255)
                                                         cATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGAc                                                                                       <  2:1244440/149‑1 (MQ=255)
                                                             gCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCaaa                                                                                   <  2:2702857/149‑1 (MQ=255)
                                                             gCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCaaa                                                                                   <  1:1052929/149‑1 (MQ=255)
                                                             gCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCaaa                                                                                   >  1:4762868/1‑149 (MQ=255)
                                                             gCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCaaa                                                                                   <  2:1534029/149‑1 (MQ=255)
                                                                aCCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACtt                                                                                >  2:2155306/1‑149 (MQ=255)
                                                                  cTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGa                                                                              >  2:4355598/1‑149 (MQ=255)
                                                                    tGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACt                                                                            >  1:1801449/1‑149 (MQ=255)
                                                                     gATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGACACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTg                                                                           <  2:1841798/149‑1 (MQ=255)
                                                                        ggCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTgggg                                                                        >  1:2354465/1‑149 (MQ=255)
                                                                        ggCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTgggg                                                                        >  1:1894546/1‑149 (MQ=255)
                                                                         gCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGc                                                                       >  2:93152/1‑149 (MQ=255)
                                                                            aCATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGtt                                                                    >  1:2236152/1‑149 (MQ=255)
                                                                              aTTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTaa                                                                  >  2:522014/1‑149 (MQ=255)
                                                                               ttCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTaaa                                                                 >  2:2198417/1‑149 (MQ=255)
                                                                                  gTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGtt                                                              <  1:139520/149‑1 (MQ=255)
                                                                                   tCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACTAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTtc                                                             >  1:3521253/1‑149 (MQ=255)
                                                                                   tCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTtc                                                             >  1:1996554/1‑149 (MQ=255)
                                                                                          aCCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCctc                                                      >  2:2324675/1‑149 (MQ=255)
                                                                                            cATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCctctc                                                    >  2:3930095/1‑149 (MQ=255)
                                                                                            cATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGAGAGCTTTCTGACCAAACTTGACTGGGGCGATAAAGTTCTCGCctctc                                                    >  1:66378/1‑149 (MQ=255)
                                                                                                cTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGa                                                >  1:2048896/1‑149 (MQ=255)
                                                                                                cTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGa                                                <  1:411981/149‑1 (MQ=255)
                                                                                                       ttGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGt                                         <  2:4637256/149‑1 (MQ=255)
                                                                                                       ttGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGt                                         <  2:2958635/149‑1 (MQ=255)
                                                                                                        tGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTc                                        <  1:3612689/149‑1 (MQ=255)
                                                                                                        tGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTc                                        <  2:1093094/149‑1 (MQ=255)
                                                                                                        tGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTc                                        <  1:1878867/149‑1 (MQ=255)
                                                                                                          gccgccAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCATACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTcgc                                      <  1:3670945/149‑1 (MQ=255)
                                                                                                          gccgccAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTcgc                                      >  2:3634822/1‑149 (MQ=255)
                                                                                                             gccAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTcgccgc                                   >  1:931302/1‑149 (MQ=255)
                                                                                                              ccAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCcgcg                                  >  2:3655920/1‑149 (MQ=255)
                                                                                                              ccAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCcgcg                                  >  2:4429326/1‑149 (MQ=255)
                                                                                                                  tataATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGc                              >  2:3763503/1‑149 (MQ=255)
                                                                                                                   ataataTCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCa                             >  1:3217/1‑149 (MQ=255)
                                                                                                                    taataTCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCAt                            >  1:2814030/1‑149 (MQ=255)
                                                                                                                       tatCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTg                         >  2:1209503/1‑149 (MQ=255)
                                                                                                                       tatCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTg                         <  1:1100102/149‑1 (MQ=255)
                                                                                                                        atCTCGCTTCCTGCACAAGCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGa                        <  1:4700656/149‑1 (MQ=255)
                                                                                                                          ctcGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGaaa                      >  2:340818/1‑149 (MQ=255)
                                                                                                                           tcGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGaaaa                     >  2:3066457/1‑149 (MQ=255)
                                                                                                                           tcGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGaaaa                     <  1:2262012/149‑1 (MQ=255)
                                                                                                                              cTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACAt                  <  1:2227939/149‑1 (MQ=255)
                                                                                                                                   tGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAg             >  1:4659021/1‑149 (MQ=255)
                                                                                                                                   tGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAg             <  2:2821933/149‑1 (MQ=255)
                                                                                                                                   tGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAg             >  1:4234585/1‑149 (MQ=255)
                                                                                                                                     cacaATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGAt           <  1:3142257/149‑1 (MQ=255)
                                                                                                                                      acaATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATg          <  1:3664017/149‑1 (MQ=255)
                                                                                                                                       caATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGc         <  2:4029566/149‑1 (MQ=255)
                                                                                                                                       caATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGc         <  1:3427517/149‑1 (MQ=255)
                                                                                                                                        aaTCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCa        >  2:3230320/1‑149 (MQ=255)
                                                                                                                                         aTCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAg       >  2:2001735/1‑149 (MQ=255)
                                                                                                                                           cccTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGcc     >  1:3327588/1‑149 (MQ=255)
                                                                                                                                             cTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCg   >  1:4503738/1‑149 (MQ=255)
                                                                                                                                             cTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCg   >  1:2849807/1‑149 (MQ=255)
                                                                                                                                             cTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCg   >  2:2365814/1‑149 (MQ=255)
                                                                                                                                              tGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGt  >  2:1886352/1‑149 (MQ=255)
                                                                                                                                              tGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGt  >  2:1400017/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TATATCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGT  >  CP009273/1696466‑1696756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑