Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,699,862 T→A 100% L119* (TTG→TAG)  ydgK → DUF2569 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,699,8620TA99.4% 1106.4 / ‑2.6 329L119* (TTG→TAG) ydgKDUF2569 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (169/157);  total (172/157)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 329 total aligned reads displayed.

TTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTT  >  CP009273/1699716‑1700007
                                                                                                                                                  |                                                                                                                                                 
ttACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGc                                                                                                                                                 >  1:4276615/1‑149 (MQ=255)
      acTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGGTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTa                                                                                                                                           <  2:4233462/149‑1 (MQ=255)
       cTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTAc                                                                                                                                          <  1:844872/149‑1 (MQ=255)
        tgtgGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACt                                                                                                                                         <  2:1398104/149‑1 (MQ=255)
        tgtgGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACt                                                                                                                                         <  1:4790134/149‑1 (MQ=255)
         gtgGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACtt                                                                                                                                        >  2:4015358/1‑149 (MQ=255)
              tGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATGTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTttgttg                                                                                                                                   <  2:2117643/149‑1 (MQ=255)
               gACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTgg                                                                                                                                  >  2:3522841/1‑149 (MQ=255)
                 ccATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCa                                                                                                                                >  1:4693228/1‑149 (MQ=255)
                  cATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCaa                                                                                                                               <  1:4108845/149‑1 (MQ=255)
                    tCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAAcc                                                                                                                             >  1:1288450/1‑149 (MQ=255)
                      gCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGc                                                                                                                           >  2:2590406/1‑149 (MQ=255)
                      gCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGc                                                                                                                           <  2:2767190/149‑1 (MQ=255)
                           ctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGa                                                                                                                      <  1:4287505/149‑1 (MQ=255)
                           ctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGa                                                                                                                      <  2:1475980/149‑1 (MQ=255)
                           ctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGa                                                                                                                      <  2:1946457/149‑1 (MQ=255)
                           ctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGa                                                                                                                      <  2:2338482/149‑1 (MQ=255)
                              tAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCg                                                                                                                   >  1:951863/1‑149 (MQ=255)
                              tAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCg                                                                                                                   <  1:1366338/149‑1 (MQ=255)
                                aaCGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTc                                                                                                                 >  2:3093136/1‑149 (MQ=255)
                                aaCGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTc                                                                                                                 <  2:4474895/149‑1 (MQ=255)
                                   gTCGGCGTTGTGTTCCTAACCACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTcccc                                                                                                              <  2:36163/149‑1 (MQ=255)
                                   gTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTcccc                                                                                                              >  1:3217508/1‑149 (MQ=255)
                                   gTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTcccc                                                                                                              <  2:534798/149‑1 (MQ=255)
                                         gTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTc                                                                                                        >  1:2469591/1‑149 (MQ=255)
                                         gTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTc                                                                                                        >  1:2868719/1‑149 (MQ=255)
                                          ttGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCa                                                                                                       <  2:469426/149‑1 (MQ=255)
                                            gtgtTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCaaa                                                                                                     <  1:2339218/149‑1 (MQ=255)
                                               ttCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGc                                                                                                  <  1:4446990/149‑1 (MQ=255)
                                                tCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCt                                                                                                 >  2:97539/1‑149 (MQ=255)
                                                tCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCt                                                                                                 >  2:3727563/1‑149 (MQ=255)
                                                    aaaCACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcgtc                                                                                             <  2:3202554/149‑1 (MQ=255)
                                                     aaCACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcgtcg                                                                                            >  1:1992750/1‑149 (MQ=255)
                                                       cacTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTcgcg                                                                                          >  1:1610081/1‑149 (MQ=255)
                                                       cacTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTcgcg                                                                                          >  1:2696917/1‑149 (MQ=255)
                                                        acTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCgg                                                                                         <  2:2059799/149‑1 (MQ=255)
                                                           atatTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTa                                                                                      >  2:1999542/1‑149 (MQ=255)
                                                           atatTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTa                                                                                      <  2:4311457/149‑1 (MQ=255)
                                                            tattatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTaa                                                                                     >  1:556044/1‑149 (MQ=255)
                                                             attatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTaaa                                                                                    >  1:2402503/1‑149 (MQ=255)
                                                               tatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAg                                                                                  <  1:2812374/149‑1 (MQ=255)
                                                                 tCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCg                                                                                >  2:1751539/1‑149 (MQ=255)
                                                                  cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGa                                                                               <  2:1509432/149‑1 (MQ=255)
                                                                     gctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGt                                                                            >  1:600179/1‑149 (MQ=255)
                                                                     gctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGt                                                                            >  2:698052/1‑149 (MQ=255)
                                                                      ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGtt                                                                           <  2:1272825/149‑1 (MQ=255)
                                                                      ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGtt                                                                           <  1:3129029/149‑1 (MQ=255)
                                                                        gctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTtg                                                                         <  2:786674/149‑1 (MQ=255)
                                                                        gctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTtg                                                                         >  2:3682575/1‑149 (MQ=255)
                                                                              ttCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATc                                                                   >  1:2768099/1‑149 (MQ=255)
                                                                              ttCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATc                                                                   >  1:1193911/1‑149 (MQ=255)
                                                                              ttCCGTATTGCTGGCAGTAAAAGCATTAGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCTACCGAATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATc                                                                   <  2:1864580/149‑1 (MQ=255)
                                                                                 cGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGt                                                                <  1:3453728/149‑1 (MQ=255)
                                                                                   tATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaa                                                              <  2:2967000/149‑1 (MQ=255)
                                                                                   tATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACAGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaa                                                              >  2:1337590/1‑149 (MQ=255)
                                                                                    aTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaat                                                             >  2:3558603/1‑149 (MQ=255)
                                                                                     ttGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaata                                                            >  1:1859166/1‑149 (MQ=255)
                                                                                        cTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAAcc                                                         >  1:2630903/1‑149 (MQ=255)
                                                                                           gCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTa                                                      >  1:1901991/1‑149 (MQ=255)
                                                                                            cAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTAc                                                     <  2:2843324/149‑1 (MQ=255)
                                                                                             aGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACa                                                    <  2:4613987/149‑1 (MQ=255)
                                                                                             aGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACa                                                    <  2:2142247/149‑1 (MQ=255)
                                                                                              gTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAg                                                   >  1:402935/1‑149 (MQ=255)
                                                                                              gTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAg                                                   >  1:54649/1‑149 (MQ=255)
                                                                                                 aaaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTa                                                >  2:885458/1‑149 (MQ=255)
                                                                                                 aaaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTa                                                >  2:3385184/1‑149 (MQ=255)
                                                                                                  aaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTaa                                               <  1:715503/149‑1 (MQ=255)
                                                                                                   aGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAc                                              >  2:4069487/1‑149 (MQ=255)
                                                                                                    gCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAcc                                             >  1:3396371/1‑149 (MQ=255)
                                                                                                       ttCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtgt                                          <  1:1584128/149‑1 (MQ=255)
                                                                                                       ttCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtgt                                          >  2:3300162/1‑149 (MQ=255)
                                                                                                          gCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtgttgt                                       >  2:2579051/1‑149 (MQ=255)
                                                                                                              tttCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGcc                                   <  1:450615/149‑1 (MQ=255)
                                                                                                                 cACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGc                                <  1:1964436/149‑1 (MQ=255)
                                                                                                                 cACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGc                                >  1:3728379/1‑149 (MQ=255)
                                                                                                                  aCCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGct                               >  1:1680088/1‑149 (MQ=255)
                                                                                                                   cccGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGctc                              <  1:3636303/149‑1 (MQ=255)
                                                                                                                   cccGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGctc                              >  2:4681355/1‑149 (MQ=255)
                                                                                                                   cccGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGctc                              >  2:3764590/1‑149 (MQ=255)
                                                                                                                        ggAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGAt                         <  1:3893104/149‑1 (MQ=255)
                                                                                                                         gAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGAtt                        <  1:2105145/149‑1 (MQ=255)
                                                                                                                          aaGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTa                       <  2:3018444/149‑1 (MQ=255)
                                                                                                                           aGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                      <  1:1128821/149‑1 (MQ=255)
                                                                                                                           aGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                      <  1:97539/149‑1 (MQ=255)
                                                                                                                           aGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                      >  1:702420/1‑149 (MQ=255)
                                                                                                                              tGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACgg                   >  1:3322575/1‑149 (MQ=255)
                                                                                                                              tGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACgg                   >  1:3838314/1‑149 (MQ=255)
                                                                                                                              tGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACgg                   >  1:3838424/1‑149 (MQ=255)
                                                                                                                               ggCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGa                  <  2:945598/149‑1 (MQ=255)
                                                                                                                                gCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGat                 >  2:3142558/1‑149 (MQ=255)
                                                                                                                                gCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGat                 <  2:2274080/149‑1 (MQ=255)
                                                                                                                                 cATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGata                <  1:3539711/149‑1 (MQ=255)
                                                                                                                                  aTAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataa               <  1:3987884/149‑1 (MQ=255)
                                                                                                                                  aTAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataa               <  1:1095727/149‑1 (MQ=255)
                                                                                                                                  aTAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataa               <  1:1598645/149‑1 (MQ=255)
                                                                                                                                     gCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAg            <  2:2651223/149‑1 (MQ=255)
                                                                                                                                       cGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATCAACGGATAATAggc          <  1:1231823/149‑1 (MQ=255)
                                                                                                                                         ttCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAggcgg        >  1:1416149/1‑149 (MQ=255)
                                                                                                                                          tCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAggcggc       <  1:4172950/149‑1 (MQ=255)
                                                                                                                                              cAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCtttt   >  1:2060821/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTT  >  CP009273/1699716‑1700007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑