Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,701,260 T→A 5.4% S29T (TCC→ACC)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,701,2600TA 5.4% 893.4 / 28.6 277S29T (TCC→ACC) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (151/110);  new base A (8/7);  total (159/118)
Fisher's exact test for biased strand distribution p-value = 7.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

CACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAA  >  CP009273/1701113‑1701407
                                                                                                                                                   |                                                                                                                                                   
   cTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCaa                                                                                                                                                 <  2:484625/149‑1 (MQ=255)
   cTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCaa                                                                                                                                                 <  2:3061310/149‑1 (MQ=255)
     gACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACg                                                                                                                                               <  1:1152876/149‑1 (MQ=255)
       cTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGt                                                                                                                                             >  1:3380304/1‑149 (MQ=255)
       cTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGt                                                                                                                                             >  1:990046/1‑149 (MQ=255)
        tCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTa                                                                                                                                            <  2:3742694/149‑1 (MQ=255)
          cTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTaca                                                                                                                                          >  2:3723810/1‑149 (MQ=255)
               aaTGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCt                                                                                                                                     >  2:61878/1‑149 (MQ=255)
                    gATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGcc                                                                                                                                <  2:345835/149‑1 (MQ=255)
                    gATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGcc                                                                                                                                <  2:1856741/149‑1 (MQ=255)
                        tGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGt                                                                                                                            >  2:2177982/1‑149 (MQ=255)
                        tGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGt                                                                                                                            <  2:2021284/149‑1 (MQ=255)
                            cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAccc                                                                                                                        >  2:4475262/1‑149 (MQ=255)
                             acCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                       >  2:854154/1‑149 (MQ=255)
                              ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAccccg                                                                                                                      >  1:3114839/1‑148 (MQ=255)
                               cATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTg                                                                                                                     <  2:3898304/149‑1 (MQ=255)
                                aTTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTgg                                                                                                                    <  1:1003166/149‑1 (MQ=255)
                                 ttCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGgc                                                                                                                   >  2:728131/1‑149 (MQ=255)
                                 ttCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGgc                                                                                                                   >  2:1309500/1‑149 (MQ=255)
                                      gTGCGTAGCATTCCCGTGGCACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGCTTTCACCGGCGGCATCCATCCCCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc                                                                                                              <  2:2368095/149‑1 (MQ=255)
                                        gagTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGt                                                                                                            <  2:113175/147‑1 (MQ=255)
                                         cGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACCCCCCTGCGCCAGGTCCCCCTGGCGCAGCggt                                                                                                           >  1:3774579/1‑147 (MQ=255)
                                         cGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGtt                                                                                                           >  2:2606027/1‑149 (MQ=255)
                                          gTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGttt                                                                                                          <  2:4677374/149‑1 (MQ=255)
                                             taaTTCCCGTGTAACACAATGATTATGTTATTCTCTGCATTCACAAAAAATAAAATCTGGGTTTTCAACGGCGGCATCCATCCACCGGATATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGTTTTGt                                                                                                       <  1:104666/147‑1 (MQ=255)
                                             tCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGTATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAATCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGt                                                                                                       >  2:3228535/1‑149 (MQ=255)
                                              cATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGtt                                                                                                      >  1:1002537/1‑149 (MQ=255)
                                                ttCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTGTTGTTAt                                                                                                    >  1:1807482/1‑149 (MQ=255)
                                                   ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTcc                                                                                                 >  1:2615220/1‑149 (MQ=255)
                                                   ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTcc                                                                                                 >  2:4193002/1‑149 (MQ=255)
                                                    cGTGTAACACCATGCTTATTTTATTCTCTGAATTCATAAAAAATAAAATCTGGGATTTCAACGGCGGCAGCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCa                                                                                                <  1:1305137/149‑1 (MQ=255)
                                                    cGTGTAACACCATGATTAAGTTATTCTCTGAATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTA‑CCCTGGCGCAGCGTTTTGTTATTCCAc                                                                                               <  1:4748614/149‑1 (MQ=255)
                                                    cGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCa                                                                                                >  1:3577526/1‑149 (MQ=255)
                                                      tGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCACGTACCCCAGGCGCAGCGTGTTGTTATTCCACt                                                                                              >  2:2837237/1‑149 (MQ=255)
                                                         aaCACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTATTCCACAGaa                                                                                           >  1:3278608/1‑149 (MQ=255)
                                                           cacCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAAc                                                                                         >  2:2034307/1‑149 (MQ=255)
                                                             ccATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAg                                                                                       >  2:2309016/1‑149 (MQ=255)
                                                                tGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCat                                                                                    >  2:934644/1‑149 (MQ=255)
                                                                tGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACAGAAACAGCag                                                                                    >  1:323853/1‑148 (MQ=255)
                                                                  cTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCatat                                                                                  >  2:1622186/1‑149 (MQ=255)
                                                                  cTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCatat                                                                                  >  2:294010/1‑149 (MQ=255)
                                                                   ttAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGGTTTGTTATTCCACTGAAACAGCatagt                                                                                 >  1:4408537/1‑147 (MQ=255)
                                                                   ttAAGTTATACTCTGCATACAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAtt                                                                                 <  2:2577254/149‑1 (MQ=255)
                                                                    tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                <  2:1782988/149‑1 (MQ=255)
                                                                    tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                >  1:363007/1‑149 (MQ=255)
                                                                     aaGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                                                               >  1:4610911/1‑149 (MQ=255)
                                                                     aaGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTgg                                                                               >  2:1012139/1‑149 (MQ=255)
                                                                       gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGCGCAGCGGTTTGTTTTTTCACGGAAACAGCATATTTgcg                                                                             >  1:4007522/1‑149 (MQ=255)
                                                                              tctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAgg                                                                      <  1:2369995/149‑1 (MQ=255)
                                                                              tctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAgg                                                                      >  2:270858/1‑149 (MQ=255)
                                                                                ggaaTTCAGAAAAAAAAAAAGCGGGGAGTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGGCCAACGGTACACCCCGGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg                                                                    <  1:2930491/146‑1 (MQ=255)
                                                                                 gaaTTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGTATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTATTCCAATTAAAAAGCATATTGGCGCATAACGTGa                                                                   >  1:2360886/3‑149 (MQ=255)
                                                                                    ttCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCCTCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGtt                                                                <  1:4344839/149‑1 (MQ=255)
                                                                                       aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgtg                                                             <  2:4405933/149‑1 (MQ=255)
                                                                                        gAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCGGAAGGTGAGTTGTGc                                                            >  1:3107668/1‑149 (MQ=255)
                                                                                        caaaaaaaaaaATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCCACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc                                                            <  1:1352364/148‑1 (MQ=255)
                                                                                          aaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGGTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGt                                                          >  2:2259646/1‑149 (MQ=255)
                                                                                          aaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTGTGTTATTACAATGAAACATAAGATTGGCGCTGAAGGTGAGTTGTTCGt                                                          >  2:2765721/1‑149 (MQ=255)
                                                                                             aataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAGATTGGCGCTGAAGGTGAGTTGTGCGTTAg                                                       >  2:3504966/1‑149 (MQ=255)
                                                                                               taaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGACGGTGAGTTGTGCGTTAGCg                                                     >  2:749650/1‑149 (MQ=255)
                                                                                               taaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCAGATTGGCGCTGAAGGTGAGTTGTGCGTTAGCg                                                     >  2:4362608/1‑149 (MQ=255)
                                                                                                aaaatCTGTGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGt                                                    <  2:4745239/149‑1 (MQ=255)
                                                                                                aaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCGGGCGCCGCGTGTTGTTATTCCACTGAAACAGCATTGTTGGGCTGAAGGTGTGTTGTTGGGTAGCGt                                                    >  1:1780702/1‑149 (MQ=255)
                                                                                                 aaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTc                                                   >  2:1460300/1‑149 (MQ=255)
                                                                                                  aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCg                                                  >  1:3131277/1‑149 (MQ=255)
                                                                                                   atCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCgg                                                 >  2:988945/1‑149 (MQ=255)
                                                                                                    tCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGc                                                >  2:3899597/1‑149 (MQ=255)
                                                                                                    tCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACACCTGCGCCAGGTACCCCAGGCGCAGCGTTTTTTTATTCCACATAAAAATCATATATGCCGTTAAGGTTATTTGTTGGTTAgaggaggc                                                >  1:3580697/1‑142 (MQ=255)
                                                                                                     cTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCg                                               <  1:2960442/149‑1 (MQ=255)
                                                                                                     cTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCg                                               >  1:1546088/1‑149 (MQ=255)
                                                                                                      tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGTAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                              >  2:1678278/1‑149 (MQ=255)
                                                                                                      tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                              >  2:1294203/1‑149 (MQ=255)
                                                                                                      tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                              <  1:3051063/149‑1 (MQ=255)
                                                                                                      tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAGATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                              >  2:1710893/1‑149 (MQ=255)
                                                                                                       gggATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGAt                                             >  2:3640969/1‑149 (MQ=255)
                                                                                                           tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg                                         <  1:1419559/149‑1 (MQ=255)
                                                                                                              cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAt                                      >  2:4577941/1‑149 (MQ=255)
                                                                                                              cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCGGAAGGTGAGGTGTGCGTTAGCGTCGGCGAGAAAGTAt                                      >  2:1060372/1‑149 (MQ=255)
                                                                                                               aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAtt                                     <  1:3811027/149‑1 (MQ=255)
                                                                                                                aCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAttt                                    >  1:3446354/1‑148 (MQ=255)
                                                                                                                 cggcggCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc                                   >  2:2142316/1‑149 (MQ=255)
                                                                                                                        atccatccACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCa                            >  1:332381/1‑149 (MQ=255)
                                                                                                                         tccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCGGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCGGGAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGcccg                           >  1:2166350/1‑147 (MQ=255)
                                                                                                                         tccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGGACCCCTGGCGCCGCGTTTTGTTATTACACTGAAAAAGCATATTGGCGCGGGAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGcccg                           >  1:1702715/1‑147 (MQ=255)
                                                                                                                         tccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAg                           >  2:1200004/1‑149 (MQ=255)
                                                                                                                          ccatccaCCGGAGATGAAAACCCAGTCCACCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGc                          <  1:691807/149‑1 (MQ=255)
                                                                                                                          ccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGc                          <  2:2441396/149‑1 (MQ=255)
                                                                                                                            atccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTTCGCGGCCAGCCg                        >  1:1948285/1‑149 (MQ=255)
                                                                                                                            atccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAACCCATGCGCAGCGTTTTTTTATTACACTTAAAAAGCAAATTGGCGCTGAAGGTTGGTTGTGGGGTAGCGTCGGGGATAAATTATTTAGCGGACAGGCg                        >  1:1948462/1‑149 (MQ=255)
                                                                                                                              ccaCCGGAGATTAAAACCCAGTCCAACGGTACACCCCTGCGCCAGTTACCCGTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCt                      <  1:1644950/149‑1 (MQ=255)
                                                                                                                              ccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCt                      <  2:3131277/149‑1 (MQ=255)
                                                                                                                              ccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCt                      <  1:205402/149‑1 (MQ=255)
                                                                                                                                aCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGGTTTGTTATTACACTGAAAAAGCATATTGGCGCGGAAGGTGAGTTGTGGGTTAGCGTCGGCCATAAAGTATTTCGCGGCCCGccccgta                    >  1:2236783/1‑144 (MQ=255)
                                                                                                                                   ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAccc                 <  1:1707702/149‑1 (MQ=255)
                                                                                                                                   ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGGATTGCGCGGCCAGCCGCTTAcac                 >  2:555409/1‑147 (MQ=255)
                                                                                                                                     agaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGt               <  1:1994181/149‑1 (MQ=255)
                                                                                                                                        tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGt            <  2:1545084/149‑1 (MQ=255)
                                                                                                                                         gAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTc           <  1:854154/149‑1 (MQ=255)
                                                                                                                                          aaaaCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTcg          <  2:437754/149‑1 (MQ=255)
                                                                                                                                                  gTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCaaaa  <  1:811372/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAA  >  CP009273/1701113‑1701407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑