Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,754,950 T→A 100% I367F (ATT→TTT)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,754,9500TA99.5% 863.1 / ‑5.6 274I367F (ATT→TTT) sufDcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (140/128);  minor base C (0/5);  total (141/133)
Fisher's exact test for biased strand distribution p-value = 2.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 274 total aligned reads displayed.

ACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGA  >  CP009273/1754804‑1755093
                                                                                                                                                  |                                                                                                                                               
aCCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGACTATCTGTTCATCATCAAAAc                                                                                                                                               >  2:3850584/1‑149 (MQ=255)
aCCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAAc                                                                                                                                               >  2:2228906/1‑149 (MQ=255)
aCCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAAc                                                                                                                                               <  2:4076861/149‑1 (MQ=255)
     ttCGGGCCAGCACCTGCTTTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGcccc                                                                                                                                          <  1:2103688/149‑1 (MQ=255)
     ttCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGcccc                                                                                                                                          >  1:2960839/1‑149 (MQ=255)
       cGGGCCAGCACCTGCTTTTTAAGCCCATCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATATAATATCTGTTCATCATCAAAACGCCCCAc                                                                                                                                        <  1:4023169/149‑1 (MQ=255)
       cGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCAc                                                                                                                                        <  1:3453130/149‑1 (MQ=255)
        gggCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCAcc                                                                                                                                       <  1:2200314/149‑1 (MQ=255)
        gggCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCAcc                                                                                                                                       >  1:1543529/1‑149 (MQ=255)
          gCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGt                                                                                                                                     <  1:3065422/149‑1 (MQ=255)
             aGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTcgc                                                                                                                                  >  2:3252946/1‑149 (MQ=255)
                  ctgctgTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGt                                                                                                                             <  2:1176568/149‑1 (MQ=255)
                     ctgTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCGGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGc                                                                                                                          <  2:3331745/149‑1 (MQ=255)
                          tAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCAt                                                                                                                     <  1:3495916/149‑1 (MQ=255)
                          tAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCAt                                                                                                                     <  2:4573530/149‑1 (MQ=255)
                            aGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCAttt                                                                                                                   <  2:1608711/149‑1 (MQ=255)
                            aGCCCCTCATCACGCAGTGCGTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGATTATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCAttt                                                                                                                   <  2:3996692/149‑1 (MQ=255)
                               cccTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTcac                                                                                                                >  2:1116947/1‑149 (MQ=255)
                                  tcatcaCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCAcatc                                                                                                             <  2:4259216/149‑1 (MQ=255)
                                    atcaCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCAcatcat                                                                                                           <  2:1101795/149‑1 (MQ=255)
                                         gCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCa                                                                                                      <  2:2696172/149‑1 (MQ=255)
                                            gTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAg                                                                                                   >  1:1861225/1‑149 (MQ=255)
                                                ttCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGAttt                                                                                               <  2:2571492/149‑1 (MQ=255)
                                                 tCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTc                                                                                              <  1:3141686/149‑1 (MQ=255)
                                                  ccGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTcc                                                                                             <  2:2356859/149‑1 (MQ=255)
                                                    gTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAg                                                                                           >  2:2097234/1‑149 (MQ=255)
                                                    gTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAg                                                                                           <  2:219414/149‑1 (MQ=255)
                                                      cAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCt                                                                                         <  1:1581799/149‑1 (MQ=255)
                                                       aGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTg                                                                                        <  2:4480837/149‑1 (MQ=255)
                                                       aGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTg                                                                                        >  1:4724653/1‑149 (MQ=255)
                                                        gTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGc                                                                                       >  1:1990824/1‑149 (MQ=255)
                                                        gTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGc                                                                                       <  2:3439636/149‑1 (MQ=255)
                                                        gTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGc                                                                                       >  2:2845556/1‑149 (MQ=255)
                                                         ttCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCg                                                                                      >  2:4378485/1‑149 (MQ=255)
                                                          tCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCgg                                                                                     <  1:3217159/149‑1 (MQ=255)
                                                             gCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGttt                                                                                  >  1:2258537/1‑149 (MQ=255)
                                                             gCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGttt                                                                                  >  1:263673/1‑149 (MQ=255)
                                                              cAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTc                                                                                 >  2:1466066/1‑149 (MQ=255)
                                                              cAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTc                                                                                 <  2:1299345/149‑1 (MQ=255)
                                                               aGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCg                                                                                <  1:4283742/149‑1 (MQ=255)
                                                                   aaGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATc                                                                            <  1:180492/149‑1 (MQ=255)
                                                                    aGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATcc                                                                           >  2:1998708/1‑149 (MQ=255)
                                                                      gCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCAc                                                                         <  2:3418715/149‑1 (MQ=255)
                                                                         tAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTc                                                                      >  2:3940107/1‑149 (MQ=255)
                                                                         tAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTc                                                                      <  2:2079531/149‑1 (MQ=255)
                                                                         tAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTc                                                                      >  1:3404023/1‑149 (MQ=255)
                                                                            aTGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGc                                                                   <  2:3612112/149‑1 (MQ=255)
                                                                             tGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGcc                                                                  >  2:2196378/1‑149 (MQ=255)
                                                                                tcatcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGt                                                               <  2:2186759/149‑1 (MQ=255)
                                                                                 catcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGtt                                                              <  1:874999/149‑1 (MQ=255)
                                                                                   tcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTg                                                            >  2:170277/1‑149 (MQ=255)
                                                                                     tgctgGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGcc                                                          <  1:974190/149‑1 (MQ=255)
                                                                                     tgctgGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGcc                                                          >  2:710428/1‑149 (MQ=255)
                                                                                        tgGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCAt                                                       <  1:1346421/149‑1 (MQ=255)
                                                                                         gggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATc                                                      <  1:3013592/149‑1 (MQ=255)
                                                                                          ggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATca                                                     >  2:2383029/1‑149 (MQ=255)
                                                                                          ggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATca                                                     <  1:2539990/149‑1 (MQ=255)
                                                                                          ggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATca                                                     <  2:535883/149‑1 (MQ=255)
                                                                                            cATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATcagc                                                   <  1:3670532/149‑1 (MQ=255)
                                                                                                ctgctgATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAt                                               <  2:1543529/149‑1 (MQ=255)
                                                                                                 tgctgATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAtt                                              <  1:2844246/149‑1 (MQ=255)
                                                                                                    tgattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgtt                                           >  2:4712825/1‑149 (MQ=255)
                                                                                                    tgattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgtt                                           <  1:1579467/149‑1 (MQ=255)
                                                                                                    tgattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgtt                                           >  2:847396/1‑149 (MQ=255)
                                                                                                     gattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgttg                                          <  1:4346870/149‑1 (MQ=255)
                                                                                                     gattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgttg                                          >  2:1884825/1‑149 (MQ=255)
                                                                                                         gatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTgg                                      >  2:2766324/1‑149 (MQ=255)
                                                                                                         gatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTgg                                      >  2:2494554/1‑149 (MQ=255)
                                                                                                          atCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGt                                     <  1:2378274/149‑1 (MQ=255)
                                                                                                           tCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTc                                    <  1:55527/149‑1 (MQ=255)
                                                                                                              cgcgcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATc                                 >  2:1067671/1‑149 (MQ=255)
                                                                                                              cgcgcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATc                                 <  1:3792610/149‑1 (MQ=255)
                                                                                                               gcgcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCt                                >  1:4760947/1‑149 (MQ=255)
                                                                                                                 gcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCCTCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGa                              <  1:3876432/149‑1 (MQ=255)
                                                                                                                 gcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGa                              <  2:1388619/149‑1 (MQ=255)
                                                                                                                 gcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGa                              <  1:753614/149‑1 (MQ=255)
                                                                                                                 gcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGa                              >  1:257546/1‑149 (MQ=255)
                                                                                                                    aGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCa                           >  1:447464/1‑149 (MQ=255)
                                                                                                                    aGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCa                           >  1:4201367/1‑149 (MQ=255)
                                                                                                                     gcgcAGATAGAATATCTGTTCATCATCAACACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATGTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCAt                          <  1:1460737/149‑1 (MQ=255)
                                                                                                                     gcgcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCAt                          <  2:960612/149‑1 (MQ=255)
                                                                                                                      cgcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATc                         >  2:4540909/1‑149 (MQ=255)
                                                                                                                       gcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATcc                        <  1:1966554/149‑1 (MQ=255)
                                                                                                                       gcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATcc                        >  2:2545196/1‑149 (MQ=255)
                                                                                                                       gcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATcc                        >  2:2958715/1‑149 (MQ=255)
                                                                                                                         aGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGt                      >  2:2649140/1‑149 (MQ=255)
                                                                                                                            tAGAATATCTGTTCATCATCAACACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGtttt                   <  1:2026430/149‑1 (MQ=255)
                                                                                                                            tAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGtttt                   >  2:1137173/1‑149 (MQ=255)
                                                                                                                              gAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGa                 <  2:2699145/149‑1 (MQ=255)
                                                                                                                              gAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGa                 >  1:692743/1‑149 (MQ=255)
                                                                                                                                     tGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGtg          >  1:3647740/1‑149 (MQ=255)
                                                                                                                                     tGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGtg          >  2:1394508/1‑149 (MQ=255)
                                                                                                                                      gTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGtgc         >  1:705641/1‑149 (MQ=255)
                                                                                                                                       ttCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACCTCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGtgct        <  2:3695500/149‑1 (MQ=255)
                                                                                                                                          atcatcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTgcg     >  1:60858/1‑149 (MQ=255)
                                                                                                                                          atcatcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTgcg     >  2:3811312/1‑149 (MQ=255)
                                                                                                                                           tcatcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTgcgc    >  1:4619037/1‑149 (MQ=255)
                                                                                                                                           tcatcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTgcgc    >  1:2437642/1‑149 (MQ=255)
                                                                                                                                             atcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGa  >  1:3257457/1‑149 (MQ=255)
                                                                                                                                             atcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGa  >  2:3463435/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGA  >  CP009273/1754804‑1755093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑