Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,825,763 C→A 100% A159A (GCG→GCT)  astC ← succinylornithine transaminase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,825,7630CA98.8% 856.8 / 0.4 253A159A (GCG→GCT) astCsuccinylornithine transaminase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (138/112);  total (139/114)
Fisher's exact test for biased strand distribution p-value = 5.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 257 total aligned reads displayed.

GACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTT  >  CP009273/1825615‑1825911
                                                                                                                                                    |                                                                                                                                                    
gACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGgc                                                                                                                                                      <  1:3827348/149‑1 (MQ=255)
     tGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCaaa                                                                                                                                                 <  2:1558794/149‑1 (MQ=255)
      gTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCaaaa                                                                                                                                                >  1:4468077/1‑149 (MQ=255)
       tAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAAt                                                                                                                                               >  1:547161/1‑149 (MQ=255)
          aaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCt                                                                                                                                            >  2:316325/1‑149 (MQ=255)
           aaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCtg                                                                                                                                           <  2:3846770/149‑1 (MQ=255)
              gcgTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGa                                                                                                                                        >  2:489762/1‑149 (MQ=255)
               cgTTGCTGGCTGTCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGaa                                                                                                                                       <  2:3760579/149‑1 (MQ=255)
                   gctggctgGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAgg                                                                                                                                   >  2:3620203/1‑149 (MQ=255)
                    ctggctggCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggc                                                                                                                                  <  2:2844847/149‑1 (MQ=255)
                    ctggctggCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggc                                                                                                                                  <  1:2126911/149‑1 (MQ=255)
                     tggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggct                                                                                                                                 <  2:2679487/149‑1 (MQ=255)
                     tggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggct                                                                                                                                 <  1:3467038/149‑1 (MQ=255)
                     tggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggct                                                                                                                                 <  2:813989/149‑1 (MQ=255)
                      ggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctg                                                                                                                                >  2:3864679/1‑149 (MQ=255)
                       gctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctgg                                                                                                                               <  1:282937/149‑1 (MQ=255)
                        ctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctggc                                                                                                                              <  2:663991/149‑1 (MQ=255)
                            caccacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGcc                                                                                                                          <  2:15277/149‑1 (MQ=255)
                             accacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGccc                                                                                                                         <  1:837416/149‑1 (MQ=255)
                             accacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGccc                                                                                                                         <  1:3148541/149‑1 (MQ=255)
                             accacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGccc                                                                                                                         <  1:392485/149‑1 (MQ=255)
                              ccacACCTCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCa                                                                                                                        <  1:874378/149‑1 (MQ=255)
                               cacaCCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCAc                                                                                                                       <  2:3299304/149‑1 (MQ=255)
                                acacCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCAcc                                                                                                                      >  1:2321745/1‑149 (MQ=255)
                                acacCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCAcc                                                                                                                      <  1:1457064/149‑1 (MQ=255)
                                  acCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCg                                                                                                                    >  1:4786682/1‑149 (MQ=255)
                                  acCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCCCGCTGGCAGAGTTAATATAGTTATAAGCAGCATTACGAATATACGCCGGCAGTGGATCAAAATACTGTGAATATGATGGATGACAAccct                                                                                                                    >  1:3817065/1‑148 (MQ=255)
                                     gCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCAc                                                                                                                 >  1:100612/1‑149 (MQ=255)
                                     gCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCAc                                                                                                                 <  2:855128/149‑1 (MQ=255)
                                                ggATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCACAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAAc                                                                                                      >  2:3645010/1‑149 (MQ=255)
                                                ggATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCACACTGACAGTAAAc                                                                                                      <  1:3304299/149‑1 (MQ=255)
                                                   tGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGc                                                                                                   <  1:2729652/149‑1 (MQ=255)
                                                   tGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGc                                                                                                   <  2:2346730/149‑1 (MQ=255)
                                                     ggTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGt                                                                                                 <  2:3485109/149‑1 (MQ=255)
                                                          gACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAc                                                                                            <  1:4379863/149‑1 (MQ=255)
                                                          gACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAc                                                                                            <  1:3007982/149‑1 (MQ=255)
                                                           aCAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAcc                                                                                           >  2:4199475/1‑149 (MQ=255)
                                                             aaTCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCAt                                                                                         <  1:2068812/149‑1 (MQ=255)
                                                              aTCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATg                                                                                        >  1:2786434/1‑149 (MQ=255)
                                                               tCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGa                                                                                       >  2:752789/1‑149 (MQ=255)
                                                               tCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGa                                                                                       <  2:2144534/149‑1 (MQ=255)
                                                               tCACCACACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGa                                                                                       >  1:3110639/1‑149 (MQ=255)
                                                                cACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGaa                                                                                      >  2:2378548/1‑149 (MQ=255)
                                                                 aCCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGaaa                                                                                     <  1:4711785/149‑1 (MQ=255)
                                                                 aCCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGaaa                                                                                     <  2:1316338/149‑1 (MQ=255)
                                                                  ccGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAAc                                                                                    >  2:3804865/1‑149 (MQ=255)
                                                                  ccGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAAc                                                                                    <  2:2396503/149‑1 (MQ=255)
                                                                  ccGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAAc                                                                                    <  1:3480539/149‑1 (MQ=255)
                                                                     cacaGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCa                                                                                 <  1:1266875/149‑1 (MQ=255)
                                                                       caGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAtt                                                                               >  2:4661236/1‑149 (MQ=255)
                                                                            agagTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGa                                                                          >  2:4426904/1‑149 (MQ=255)
                                                                                tcgtcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGc                                                                      >  2:93656/1‑149 (MQ=255)
                                                                                tcgtcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGc                                                                      >  2:1755969/1‑149 (MQ=255)
                                                                                tcgtcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGc                                                                      >  2:4183793/1‑149 (MQ=255)
                                                                                   tcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCAc                                                                   <  2:692478/149‑1 (MQ=255)
                                                                                   tcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCAc                                                                   >  2:4725074/1‑149 (MQ=255)
                                                                                      aTCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGAt                                                                >  2:1341624/1‑149 (MQ=255)
                                                                                      aTCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGAt                                                                <  1:3381328/149‑1 (MQ=255)
                                                                                         aGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGcc                                                             >  1:1118821/1‑149 (MQ=255)
                                                                                            gcgcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGct                                                          >  2:1147927/1‑149 (MQ=255)
                                                                                              gcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGctct                                                        <  2:2840330/149‑1 (MQ=255)
                                                                                              gcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGctct                                                        >  1:3423189/1‑149 (MQ=255)
                                                                                               cTGGCAGAGTTAATATCGTTATATGCAGCATGACTAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCtt                                                       <  1:3194169/149‑1 (MQ=255)
                                                                                                tGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCTGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTa                                                      >  1:3240531/1‑149 (MQ=255)
                                                                                                 ggCAGAGTTAATATCGTTATATGCATCATGACGAATATCAGCCTCCAGTGGAGCAAAAACCTGTGAATAGGCTGGCAGCCCACCCGAACTGAAAGTAAACAGCGTGCGACCATGAAACGCATTATTGAACTACACGATGCCGATCTTAt                                                     >  2:617851/1‑149 (MQ=255)
                                                                                                 ggCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                     >  1:1075098/1‑149 (MQ=255)
                                                                                                 ggCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                     <  1:3890633/149‑1 (MQ=255)
                                                                                                 ggCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                     >  1:923298/1‑149 (MQ=255)
                                                                                                 ggCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                     >  1:780686/1‑149 (MQ=255)
                                                                                                  gCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATg                                                    >  2:3041218/1‑149 (MQ=255)
                                                                                                  gCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATg                                                    <  2:4507085/149‑1 (MQ=255)
                                                                                                   cAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATgg                                                   >  1:2019457/1‑149 (MQ=255)
                                                                                                     gagTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCt                                                 >  1:1629620/1‑149 (MQ=255)
                                                                                                           atatCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTa                                           >  2:1758581/1‑149 (MQ=255)
                                                                                                           atatCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTa                                           >  2:449891/1‑149 (MQ=255)
                                                                                                              tCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCg                                        >  2:1922021/1‑149 (MQ=255)
                                                                                                              tCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCg                                        >  2:3845481/1‑149 (MQ=255)
                                                                                                                gTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCCTGAAACGCATGTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGt                                      >  1:365519/1‑149 (MQ=255)
                                                                                                                gTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGt                                      <  1:1209430/149‑1 (MQ=255)
                                                                                                                gTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGt                                      >  1:2757106/1‑149 (MQ=255)
                                                                                                                 ttATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTc                                     >  2:2848556/1‑149 (MQ=255)
                                                                                                                  tataTGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCg                                    >  2:2491596/1‑149 (MQ=255)
                                                                                                                   atatGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGt                                   >  1:2461609/1‑149 (MQ=255)
                                                                                                                    tatGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTg                                  >  2:1099847/1‑149 (MQ=255)
                                                                                                                    tatGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTg                                  <  1:4050359/149‑1 (MQ=255)
                                                                                                                    tatGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTg                                  >  2:1245689/1‑149 (MQ=255)
                                                                                                                     atGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGa                                 >  1:4115878/1‑149 (MQ=255)
                                                                                                                      tGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAg                                >  1:3196438/1‑149 (MQ=255)
                                                                                                                       gcagcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGc                               >  2:1029939/1‑149 (MQ=255)
                                                                                                                       gcagcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGc                               <  2:3222696/149‑1 (MQ=255)
                                                                                                                          gcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGaa                            >  1:1099658/1‑149 (MQ=255)
                                                                                                                            aTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAtt                          >  1:4132078/1‑149 (MQ=255)
                                                                                                                            aTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAtt                          >  1:2621037/1‑149 (MQ=255)
                                                                                                                            aTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAtt                          <  1:220730/149‑1 (MQ=255)
                                                                                                                            aTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAtt                          <  2:1484378/149‑1 (MQ=255)
                                                                                                                             tGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAttt                         >  2:1960318/1‑149 (MQ=255)
                                                                                                                                   atatCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACgcgc                   <  2:2055716/149‑1 (MQ=255)
                                                                                                                                     atCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACgcgcga                 >  2:4711005/1‑147 (MQ=255)
                                                                                                                                        cgccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGtt              <  1:801003/149‑1 (MQ=255)
                                                                                                                                              cAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAgcg        <  1:2335574/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTT  >  CP009273/1825615‑1825911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑