Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,833,730 C→A 100% R3S (CGT→AGT)  ynjE → molybdopterin synthase sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,833,7300CA97.8% 1039.3 / 10.1 320R3S (CGT→AGT) ynjEmolybdopterin synthase sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (6/1);  new base A (178/133);  total (185/135)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 321 total aligned reads displayed.

TCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTTAT  >  CP009273/1833584‑1833876
                                                                                                                                                  |                                                                                                                                                  
tcCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAgt                                                                                                                                                  >  2:2085314/1‑149 (MQ=255)
   aGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGtt                                                                                                                                               >  1:3086321/1‑149 (MQ=255)
   aGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGtt                                                                                                                                               <  1:984438/149‑1 (MQ=255)
     gATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTtc                                                                                                                                             <  1:1144678/149‑1 (MQ=255)
     gATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTtc                                                                                                                                             >  1:2300228/1‑149 (MQ=255)
      aTGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTtct                                                                                                                                            <  1:3617183/149‑1 (MQ=255)
      aTGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTtct                                                                                                                                            <  2:1094043/149‑1 (MQ=255)
       tGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTtctc                                                                                                                                           >  2:374335/1‑149 (MQ=255)
       tGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTtctc                                                                                                                                           >  1:4579625/1‑149 (MQ=255)
       tGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTtctc                                                                                                                                           <  1:1866964/149‑1 (MQ=255)
           ccTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAAt                                                                                                                                       <  2:4354242/149‑1 (MQ=255)
             tgctgATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGa                                                                                                                                     >  1:2921406/1‑149 (MQ=255)
             tgctgATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGa                                                                                                                                     <  2:2683277/149‑1 (MQ=255)
               ctgATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGAcc                                                                                                                                   >  1:2548286/1‑149 (MQ=255)
               ctgATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGAcc                                                                                                                                   >  2:652621/1‑149 (MQ=255)
                 gATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACcgc                                                                                                                                 >  2:2310907/1‑149 (MQ=255)
                   tAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCgcgc                                                                                                                               >  1:1482388/1‑149 (MQ=255)
                         tgttctgGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCaa                                                                                                                         >  2:3089081/1‑149 (MQ=255)
                            tctgGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACCATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATgg                                                                                                                      >  2:1003216/1‑149 (MQ=255)
                            tctgGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATgg                                                                                                                      <  1:2833821/149‑1 (MQ=255)
                                gATATGGCGCAGTTGTCAGAAATTTACAATTTACTGCGATAACGGATAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTGAAATCTACACTGTCGGGATTTTCGATGAAACGTTTTTCTCAAATGACCGGGATTGCTATGTCttt                                                                                                                  <  2:503851/149‑1 (MQ=255)
                                    tGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAggg                                                                                                              <  1:3623384/149‑1 (MQ=255)
                                      gcgcAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGggct                                                                                                            >  1:289323/1‑149 (MQ=255)
                                        gcAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGggctgg                                                                                                          <  2:4623452/149‑1 (MQ=255)
                                         cAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGggctggc                                                                                                         <  1:2734543/149‑1 (MQ=255)
                                           gTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCtt                                                                                                       <  2:371720/149‑1 (MQ=255)
                                           gTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCtt                                                                                                       >  1:3042139/1‑149 (MQ=255)
                                              gTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTgcg                                                                                                    >  2:1118356/1‑149 (MQ=255)
                                              gTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTgcg                                                                                                    >  2:4336764/1‑149 (MQ=255)
                                               tCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTgcgc                                                                                                   <  1:2987664/149‑1 (MQ=255)
                                                   aaaaTTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGcttct                                                                                               <  2:141401/149‑1 (MQ=255)
                                                   aaaaTTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGcttct                                                                                               <  2:2533357/149‑1 (MQ=255)
                                                       ttACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGt                                                                                           <  1:4733133/149‑1 (MQ=255)
                                                         acaacaAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTgg                                                                                         <  1:2069359/149‑1 (MQ=255)
                                                         acaacaAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGAGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTgg                                                                                         >  1:1997275/1‑149 (MQ=255)
                                                          caacaaACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTggg                                                                                        >  1:1606353/1‑149 (MQ=255)
                                                           aacaaACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGc                                                                                       <  2:1343111/149‑1 (MQ=255)
                                                            acaaACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAATGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGcc                                                                                      >  2:2987029/1‑149 (MQ=255)
                                                                aCTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTg                                                                                  <  1:4473668/149‑1 (MQ=255)
                                                                aCTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTg                                                                                  <  2:4465410/149‑1 (MQ=255)
                                                                     gATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTg                                                                             <  1:4047274/149‑1 (MQ=255)
                                                                       tAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGc                                                                           >  1:1256998/1‑149 (MQ=255)
                                                                        aaCGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCg                                                                          >  1:2622794/1‑149 (MQ=255)
                                                                        aaCGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCg                                                                          >  1:4683959/1‑149 (MQ=255)
                                                                         aCGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGa                                                                         >  1:2395734/1‑149 (MQ=255)
                                                                            cAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGc                                                                      >  2:92333/1‑149 (MQ=255)
                                                                            cAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGc                                                                      <  1:4004143/149‑1 (MQ=255)
                                                                             aaaGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGcc                                                                     >  2:3418916/1‑149 (MQ=255)
                                                                              aaGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCct                                                                    <  1:383137/149‑1 (MQ=255)
                                                                               aGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCctc                                                                   >  2:1741259/1‑149 (MQ=255)
                                                                                gTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCctct                                                                  <  1:999568/149‑1 (MQ=255)
                                                                                 tttttCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCtt                                                                 >  1:373894/1‑149 (MQ=255)
                                                                                  ttttCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTa                                                                <  1:3549037/149‑1 (MQ=255)
                                                                                       tcAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACtt                                                           >  2:3894043/1‑149 (MQ=255)
                                                                                        cAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTg                                                          >  2:3241170/1‑149 (MQ=255)
                                                                                         actGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGa                                                         <  2:2777375/147‑1 (MQ=255)
                                                                                          aTGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGAc                                                        >  1:339183/1‑149 (MQ=255)
                                                                                           ggCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGAcc                                                       <  2:2145422/148‑1 (MQ=255)
                                                                                               tCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCt                                                   >  1:56261/1‑149 (MQ=255)
                                                                                                cAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCtt                                                  >  2:303529/1‑149 (MQ=255)
                                                                                                 aGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTc                                                 <  2:3284123/149‑1 (MQ=255)
                                                                                                 aGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTc                                                 <  2:2031048/149‑1 (MQ=255)
                                                                                                  gTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTca                                                >  1:3365935/1‑149 (MQ=255)
                                                                                                   ttCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaa                                               <  1:3328257/149‑1 (MQ=255)
                                                                                                    tCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaac                                              >  2:760140/1‑149 (MQ=255)
                                                                                                     cAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaaca                                             >  2:4243993/1‑149 (MQ=255)
                                                                                                       gAATGGCGCACTCAAAACTACACTGTCGGGATTTTCGATGAAAAGTGTTTCTCAACTGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaacaac                                           <  2:2902727/149‑1 (MQ=255)
                                                                                                         aTGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaacaacaa                                         >  1:77128/1‑149 (MQ=255)
                                                                                                         aTGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaacaacaa                                         >  1:3207956/1‑149 (MQ=255)
                                                                                                              gcACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATgg                                    <  1:1037463/149‑1 (MQ=255)
                                                                                                                aCTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCa                                  >  2:593192/1‑149 (MQ=255)
                                                                                                                 cTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCaa                                 <  1:152136/149‑1 (MQ=255)
                                                                                                                 cTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCaa                                 <  1:865077/149‑1 (MQ=255)
                                                                                                                  tCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCaaa                                <  2:3852965/149‑1 (MQ=255)
                                                                                                                  tCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCaaa                                >  2:3425505/1‑149 (MQ=255)
                                                                                                                  tCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCaaa                                <  2:1770242/149‑1 (MQ=255)
                                                                                                                   cAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAg                               <  2:844611/149‑1 (MQ=255)
                                                                                                                    aaaaCTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGc                              <  1:1737491/149‑1 (MQ=255)
                                                                                                                     aaaCTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCg                             >  2:4165704/1‑149 (MQ=255)
                                                                                                                        cTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgata                          <  2:1291072/149‑1 (MQ=255)
                                                                                                                        cTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgata                          <  2:3774725/149‑1 (MQ=255)
                                                                                                                         tACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgatag                         >  2:3163103/1‑149 (MQ=255)
                                                                                                                          aCAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGATGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgataga                        >  2:4448226/1‑149 (MQ=255)
                                                                                                                          aCAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgataga                        <  1:3551751/149‑1 (MQ=255)
                                                                                                                          aCAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgataga                        <  2:1809513/149‑1 (MQ=255)
                                                                                                                             aTGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATAc                     >  1:4132538/1‑149 (MQ=255)
                                                                                                                             aTGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATAc                     <  1:2436320/149‑1 (MQ=255)
                                                                                                                              tGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACt                    >  2:801148/1‑149 (MQ=255)
                                                                                                                               gTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTc                   >  1:3888328/1‑149 (MQ=255)
                                                                                                                               gTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTc                   >  2:1600623/1‑149 (MQ=255)
                                                                                                                                tCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCg                  >  1:2177468/1‑149 (MQ=255)
                                                                                                                                   gtaTTTTCTATTAAATGTGTTTCTAAAATTACCGCGCTGGCAATGGATTTAGGGCTGGCTTGCGCTTCTTCTTGGGCCGCTGAACTGGC‑TAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGcccc              <  1:2310907/147‑1 (MQ=255)
                                                                                                                                   ggATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGccc               >  1:515062/1‑149 (MQ=255)
                                                                                                                                      ttttCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAg            <  2:913898/149‑1 (MQ=255)
                                                                                                                                      ttttCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAg            <  2:2878429/149‑1 (MQ=255)
                                                                                                                                           gATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGt       >  1:4369496/1‑149 (MQ=255)
                                                                                                                                            aTGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGtt      <  2:2291377/149‑1 (MQ=255)
                                                                                                                                            aTGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGtt      >  1:3159940/1‑149 (MQ=255)
                                                                                                                                             tGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGttt     <  2:4677685/149‑1 (MQ=255)
                                                                                                                                             tGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGttt     >  2:1902640/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTTAT  >  CP009273/1833584‑1833876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑