Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,865,422 C→A 100% R261R (CGA→AGA)  yeaI → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,865,4220CA99.6% 968.8 / ‑4.4 281R261R (CGA→AGA) yeaIputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (137/143);  total (137/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 282 total aligned reads displayed.

ACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGT  >  CP009273/1865276‑1865567
                                                                                                                                                  |                                                                                                                                                 
acaATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGa                                                                                                                                                 >  2:215275/1‑149 (MQ=255)
 caATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCGGGGGACTCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGAt                                                                                                                                                >  1:3570371/1‑149 (MQ=255)
   aTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTa                                                                                                                                              >  1:3418254/1‑149 (MQ=255)
    tCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAg                                                                                                                                             <  1:1001456/149‑1 (MQ=255)
     cTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTCGc                                                                                                                                            <  1:1121498/149‑1 (MQ=255)
          cAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTATCCAGCg                                                                                                                                       >  1:1338785/1‑149 (MQ=255)
            gTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGaa                                                                                                                                     <  2:1511876/149‑1 (MQ=255)
             ttATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAAc                                                                                                                                    >  1:4339315/1‑149 (MQ=255)
              tataACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACt                                                                                                                                   >  1:3137127/1‑149 (MQ=255)
               ataACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTa                                                                                                                                  <  2:915889/149‑1 (MQ=255)
                taACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTAt                                                                                                                                 <  1:721768/149‑1 (MQ=255)
                 aaCGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTATTTATTATCATGCGCACAAGATTAGCCAGCGAACTATg                                                                                                                                >  1:3307420/1‑149 (MQ=255)
                 aaCGCTGACTATTCTTTGTATGTCGTCGAGTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATg                                                                                                                                >  1:4418257/1‑149 (MQ=255)
                  aCGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATgg                                                                                                                               >  1:1591073/1‑149 (MQ=255)
                      tGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGt                                                                                                                           >  1:1264763/1‑149 (MQ=255)
                        aCTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTa                                                                                                                         <  2:3530478/149‑1 (MQ=255)
                           aTTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATa                                                                                                                      <  1:2218235/149‑1 (MQ=255)
                           aTTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATa                                                                                                                      >  2:2977702/1‑149 (MQ=255)
                             tCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGc                                                                                                                    >  2:3990872/1‑149 (MQ=255)
                                  gTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCattat                                                                                                               >  1:3449412/1‑149 (MQ=255)
                                  gTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCaatat                                                                                                               >  1:3712603/1‑149 (MQ=255)
                                   tATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATATCattatt                                                                                                              >  1:2835119/1‑149 (MQ=255)
                                    aTGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCAttatta                                                                                                             <  1:182540/149‑1 (MQ=255)
                                    aTGTCGTAGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCAttatta                                                                                                             <  2:4642680/149‑1 (MQ=255)
                                       tcgtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGt                                                                                                          >  2:1028545/1‑149 (MQ=255)
                                        cgtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTc                                                                                                         <  1:3116219/149‑1 (MQ=255)
                                        cgtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTc                                                                                                         >  2:1686656/1‑149 (MQ=255)
                                          tcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTg                                                                                                       >  2:4322604/1‑149 (MQ=255)
                                          tcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTg                                                                                                       >  2:3771402/1‑149 (MQ=255)
                                            gATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGc                                                                                                     >  2:3694400/1‑149 (MQ=255)
                                             aTTACTGTCCGGACAACCATCCTGCGACCTGGGGAATCAACTATACAAAAATAGTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCCTGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCa                                                                                                    <  2:424403/149‑1 (MQ=255)
                                             aTTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCa                                                                                                    >  1:2084528/1‑149 (MQ=255)
                                             aTTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCa                                                                                                    <  1:149718/149‑1 (MQ=255)
                                               tACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATc                                                                                                  <  2:1618560/149‑1 (MQ=255)
                                                 cTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGc                                                                                                <  2:2553026/149‑1 (MQ=255)
                                                 cTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGc                                                                                                <  2:6546/149‑1 (MQ=255)
                                                   gTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTa                                                                                              <  1:530192/149‑1 (MQ=255)
                                                   gTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTa                                                                                              <  2:1285628/149‑1 (MQ=255)
                                                     ccGGACAACCAGACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAtg                                                                                            <  2:4680283/149‑1 (MQ=255)
                                                      cGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAtgc                                                                                           <  1:2756378/149‑1 (MQ=255)
                                                         aCAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAtgctgc                                                                                        <  1:138706/149‑1 (MQ=255)
                                                          cAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCa                                                                                       <  1:1969498/149‑1 (MQ=255)
                                                             ccATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAact                                                                                    >  2:4687870/1‑149 (MQ=255)
                                                              cATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAactt                                                                                   >  1:3182306/1‑149 (MQ=255)
                                                                   tGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTc                                                                              <  2:4582007/149‑1 (MQ=255)
                                                                   tGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTc                                                                              <  2:3266106/149‑1 (MQ=255)
                                                                    gCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCt                                                                             >  2:4629637/1‑149 (MQ=255)
                                                                     cGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTa                                                                            <  2:1196462/149‑1 (MQ=255)
                                                                      gACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAc                                                                           <  1:4781262/149‑1 (MQ=255)
                                                                      gACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAc                                                                           <  2:4185169/149‑1 (MQ=255)
                                                                      gACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAc                                                                           <  2:3611037/149‑1 (MQ=255)
                                                                          tGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGAc                                                                       <  2:3036471/149‑1 (MQ=255)
                                                                              gAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTg                                                                   >  1:2520658/1‑149 (MQ=255)
                                                                              gAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTg                                                                   <  1:3894545/149‑1 (MQ=255)
                                                                                aTCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGa                                                                 <  1:4020681/149‑1 (MQ=255)
                                                                                aTCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGa                                                                 <  2:257326/149‑1 (MQ=255)
                                                                                aTCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGa                                                                 <  2:3174953/149‑1 (MQ=255)
                                                                                 tCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGAt                                                                >  1:2638403/1‑149 (MQ=255)
                                                                                  cAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATg                                                               >  2:4538587/1‑149 (MQ=255)
                                                                                   aaCTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGa                                                              >  1:1907531/1‑149 (MQ=255)
                                                                                   aaCTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGa                                                              <  2:2090618/149‑1 (MQ=255)
                                                                                      tataCAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGta                                                           >  2:833135/1‑149 (MQ=255)
                                                                                         aCAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATaa                                                        >  1:4439235/1‑149 (MQ=255)
                                                                                          cAAAAATATTGGTTTGTCTGTGGGCATTTTTACTTTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAAt                                                       <  2:3073202/149‑1 (MQ=255)
                                                                                          cAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAAt                                                       >  2:1803320/1‑149 (MQ=255)
                                                                                             aaaTATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATtat                                                    >  1:2636342/1‑149 (MQ=255)
                                                                                               atatTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATAc                                                  >  2:3474283/1‑149 (MQ=255)
                                                                                                 atTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGAATCTGGATGAGTATAATTATACTa                                                >  2:3839417/1‑149 (MQ=255)
                                                                                                  ttGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTAt                                               <  2:2291655/149‑1 (MQ=255)
                                                                                                    ggTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCt                                             >  1:427388/1‑149 (MQ=255)
                                                                                                       ttGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGt                                          >  1:1714044/1‑149 (MQ=255)
                                                                                                         gTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGtat                                        >  2:3386164/1‑149 (MQ=255)
                                                                                                           cTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTatat                                      <  2:2750527/149‑1 (MQ=255)
                                                                                                            tgtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATc                                     <  2:2949645/149‑1 (MQ=255)
                                                                                                            tgtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATc                                     >  2:884342/1‑149 (MQ=255)
                                                                                                            tgtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATc                                     <  2:3334605/149‑1 (MQ=255)
                                                                                                            tgtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATAATATCTGGTATATc                                     >  2:3885758/1‑149 (MQ=255)
                                                                                                             gtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCa                                    >  2:4739221/1‑149 (MQ=255)
                                                                                                             gtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTAACTTCTACTGACTCTGGATGAGTATAATTATACTATCTTGTATATCa                                    >  2:3496087/1‑149 (MQ=255)
                                                                                                              tgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAg                                   >  1:4224034/1‑149 (MQ=255)
                                                                                                                    tttttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCgg                             >  2:1416211/1‑149 (MQ=255)
                                                                                                                       ttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAt                          <  1:3534198/149‑1 (MQ=255)
                                                                                                                       ttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAt                          <  1:871349/149‑1 (MQ=255)
                                                                                                                        tACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAtt                         <  1:390277/149‑1 (MQ=255)
                                                                                                                        tACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAtt                         <  1:786080/149‑1 (MQ=255)
                                                                                                                         aCTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGATAATAGCATTATTATGTCAGGCATCCCTATGCTGCAACTTACTACAACAGACTCTGGATGAGTATAAATATACAATCTGGTATATAAGACGCCGGAGTg                        >  1:233304/1‑149 (MQ=255)
                                                                                                                          cTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGa                       <  2:2016191/149‑1 (MQ=255)
                                                                                                                           tGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGaa                      >  2:3373058/1‑149 (MQ=255)
                                                                                                                            gTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTAACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAg                     >  1:3880430/1‑149 (MQ=255)
                                                                                                                              tCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGtt                   >  1:660126/1‑149 (MQ=255)
                                                                                                                               cTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGttt                  >  2:4081496/1‑149 (MQ=255)
                                                                                                                                tttATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTc                 <  2:1133219/149‑1 (MQ=255)
                                                                                                                                    ttatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGt             >  2:2678103/1‑149 (MQ=255)
                                                                                                                                      atCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTTTGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATAAGTCGCGGGATTGAAGTTTCCAGTaa           >  2:2038901/1‑149 (MQ=255)
                                                                                                                                       tCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaaa          <  2:2242579/149‑1 (MQ=255)
                                                                                                                                       tCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaaa          <  1:3951360/149‑1 (MQ=255)
                                                                                                                                           gcgcACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgt      <  1:2977702/149‑1 (MQ=255)
                                                                                                                                              cacaAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtttg   >  2:481520/1‑149 (MQ=255)
                                                                                                                                              cacaAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtttg   >  1:1408657/1‑149 (MQ=255)
                                                                                                                                               acaaGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtttgt  >  2:3228802/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGT  >  CP009273/1865276‑1865567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑