Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 158,287 C→A 100% D78Y (GAC→TAC)  ligT ← 2'‑5' RNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273158,2870CA100.0% 1126.8 / NA 325D78Y (GAC→TAC) ligT2'‑5' RNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (156/169);  total (156/169)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 335 total aligned reads displayed.

AATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGG  >  CP009273/158141‑158431
                                                                                                                                                  |                                                                                                                                                
aaTATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaat                                                                                                                                                >  1:1963894/1‑149 (MQ=255)
 atatGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaatc                                                                                                                                               <  2:2134922/149‑1 (MQ=255)
  tatGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaatca                                                                                                                                              <  1:440259/149‑1 (MQ=255)
  tatGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaatca                                                                                                                                              >  2:1570960/1‑149 (MQ=255)
     gtgGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGc                                                                                                                                           <  1:4260016/149‑1 (MQ=255)
     gtgGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGc                                                                                                                                           <  1:2733269/149‑1 (MQ=255)
         aTGAAACGGACGATTGCTTTGAGAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGa                                                                                                                                       <  1:4231384/149‑1 (MQ=255)
          tGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAg                                                                                                                                      <  2:931587/149‑1 (MQ=255)
          tGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAg                                                                                                                                      >  1:2491254/1‑149 (MQ=255)
           gAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAgt                                                                                                                                     >  2:4380225/1‑149 (MQ=255)
              aCGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGtgtg                                                                                                                                  >  1:2718720/1‑149 (MQ=255)
              aCGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGtgtg                                                                                                                                  >  2:2576271/1‑149 (MQ=255)
                ggACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGaa                                                                                                                                >  2:310235/1‑149 (MQ=255)
                 gACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGaaa                                                                                                                               <  2:734662/149‑1 (MQ=255)
                 gACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGaaa                                                                                                                               >  2:4234235/1‑149 (MQ=255)
                 gACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGaaa                                                                                                                               <  2:3125084/149‑1 (MQ=255)
                  aCGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAAc                                                                                                                              <  2:2966641/149‑1 (MQ=255)
                  aCGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAAc                                                                                                                              <  1:1473345/149‑1 (MQ=255)
                  aCGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAAc                                                                                                                              >  2:3221901/1‑149 (MQ=255)
                   cGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAAcc                                                                                                                             >  1:1943403/1‑149 (MQ=255)
                   cGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAAcc                                                                                                                             <  2:2321206/149‑1 (MQ=255)
                   cGAGTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAAcc                                                                                                                             <  2:4678817/149‑1 (MQ=255)
                    gATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCa                                                                                                                            <  2:3768012/149‑1 (MQ=255)
                    gATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCa                                                                                                                            <  2:1247715/149‑1 (MQ=255)
                    gATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCa                                                                                                                            <  1:4721767/149‑1 (MQ=255)
                    gATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCa                                                                                                                            <  2:1629965/149‑1 (MQ=255)
                      ttGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAgg                                                                                                                          <  2:1807845/149‑1 (MQ=255)
                      ttGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAgg                                                                                                                          <  2:1293043/149‑1 (MQ=255)
                      ttGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAgg                                                                                                                          >  1:3855750/1‑149 (MQ=255)
                       tGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGt                                                                                                                         >  1:2822274/1‑149 (MQ=255)
                         cTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTg                                                                                                                       >  1:4678759/1‑149 (MQ=255)
                          tttGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGa                                                                                                                      <  1:636990/149‑1 (MQ=255)
                          tttGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGa                                                                                                                      >  1:4113941/1‑149 (MQ=255)
                            tGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACg                                                                                                                    <  1:770463/149‑1 (MQ=255)
                              aaaaCAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGaa                                                                                                                  <  2:3599899/149‑1 (MQ=255)
                               aaaCAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAt                                                                                                                 >  2:4476329/1‑149 (MQ=255)
                                aacaacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtc                                                                                                                <  2:566356/149‑1 (MQ=255)
                                   aacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgt                                                                                                             >  2:3664479/1‑149 (MQ=255)
                                    acCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgtc                                                                                                            >  1:689022/1‑149 (MQ=255)
                                         tGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCt                                                                                                       <  2:1724182/149‑1 (MQ=255)
                                         tGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCt                                                                                                       <  2:770330/149‑1 (MQ=255)
                                          gCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTa                                                                                                      >  1:898351/1‑149 (MQ=255)
                                           cGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaa                                                                                                     >  1:3046448/1‑149 (MQ=255)
                                             ggCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaaaa                                                                                                   <  1:4616227/149‑1 (MQ=255)
                                             ggCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaaaa                                                                                                   >  2:3726665/1‑149 (MQ=255)
                                             ggCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaaaa                                                                                                   <  1:173/149‑1 (MQ=255)
                                              gCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAag                                                                                                  >  1:2885925/1‑149 (MQ=255)
                                               cAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAaga                                                                                                 <  1:4552388/149‑1 (MQ=255)
                                               cAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAaga                                                                                                 <  2:2283850/149‑1 (MQ=255)
                                                aGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAagag                                                                                                >  2:345784/1‑149 (MQ=255)
                                                 gCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACTCGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAgaga                                                                                               <  1:3444355/149‑1 (MQ=255)
                                                 gCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAgaga                                                                                               >  1:2111794/1‑149 (MQ=255)
                                                      tgAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcg                                                                                          >  1:1575142/1‑149 (MQ=255)
                                                           ggAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtc                                                                                     >  2:3358672/1‑149 (MQ=255)
                                                            gAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAACCCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtct                                                                                    >  1:4452435/1‑149 (MQ=255)
                                                            gAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtct                                                                                    >  1:1739646/1‑149 (MQ=255)
                                                            gAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtct                                                                                    <  2:2693052/149‑1 (MQ=255)
                                                            gAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtct                                                                                    <  2:2915427/149‑1 (MQ=255)
                                                             aGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtctc                                                                                   <  1:1251017/149‑1 (MQ=255)
                                                              gCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTctct                                                                                  >  2:3988200/1‑149 (MQ=255)
                                                               cATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCtt                                                                                 >  1:3293727/1‑149 (MQ=255)
                                                                   ttCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtctc                                                                             <  1:3342240/149‑1 (MQ=255)
                                                                        cAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGgcgc                                                                        <  1:993291/149‑1 (MQ=255)
                                                                          gCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTc                                                                      >  1:1676671/1‑149 (MQ=255)
                                                                          gCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTc                                                                      >  2:2108376/1‑149 (MQ=255)
                                                                           cTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCa                                                                     <  2:4729239/149‑1 (MQ=255)
                                                                           cTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGAGTAATCAAGCGTGATTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCa                                                                     <  1:4483228/149‑1 (MQ=255)
                                                                             ggATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACt                                                                   <  1:3990684/149‑1 (MQ=255)
                                                                             ggATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACt                                                                   >  2:4193698/1‑149 (MQ=255)
                                                                                  aaGCCGCGTGGGGGGTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGcc                                                              <  1:4780600/149‑1 (MQ=255)
                                                                                              ggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCag                                                  >  2:1448519/1‑149 (MQ=255)
                                                                                              ggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCag                                                  >  1:4439047/1‑149 (MQ=255)
                                                                                               gcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCaga                                                 <  2:2822153/149‑1 (MQ=255)
                                                                                                cTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCagag                                                <  2:485709/149‑1 (MQ=255)
                                                                                                cTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCagag                                                <  2:237398/149‑1 (MQ=255)
                                                                                                  gACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTc                                              >  1:239442/1‑149 (MQ=255)
                                                                                                   aCGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCa                                             >  2:1048240/1‑149 (MQ=255)
                                                                                                      cATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGAt                                          >  1:156691/1‑149 (MQ=255)
                                                                                                        tCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGc                                        <  1:2677433/149‑1 (MQ=255)
                                                                                                             aaCCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAAtt                                   <  1:1326484/149‑1 (MQ=255)
                                                                                                               ccacaccacaCGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTAt                                 >  1:3200953/1‑149 (MQ=255)
                                                                                                                 acaccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcg                               >  1:3686532/1‑149 (MQ=255)
                                                                                                                    ccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcggcg                            <  2:2765184/149‑1 (MQ=255)
                                                                                                                    ccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcggcg                            <  1:3704947/149‑1 (MQ=255)
                                                                                                                    ccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcggcg                            <  1:2775023/149‑1 (MQ=255)
                                                                                                                    ccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcggcg                            >  2:2243471/1‑149 (MQ=255)
                                                                                                                       cacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGgcggcg                         >  1:123543/1‑149 (MQ=255)
                                                                                                                          gcgAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGAcc                      <  1:1012683/149‑1 (MQ=255)
                                                                                                                          gcgAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGAcc                      >  1:3563709/1‑149 (MQ=255)
                                                                                                                            gAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCgg                    >  2:1922767/1‑149 (MQ=255)
                                                                                                                            gAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCgg                    >  2:808965/1‑149 (MQ=255)
                                                                                                                             aaCGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGa                   >  2:878169/1‑149 (MQ=255)
                                                                                                                             aaCGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGa                   <  1:1122264/149‑1 (MQ=255)
                                                                                                                             aaCGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGa                   <  1:2566819/149‑1 (MQ=255)
                                                                                                                              aCGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGAc                  <  1:1907755/149‑1 (MQ=255)
                                                                                                                              aCGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGAc                  >  1:2020071/1‑149 (MQ=255)
                                                                                                                                      aTTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGTCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGcc          >  2:594633/1‑149 (MQ=255)
                                                                                                                                       ttGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCt         >  1:1594435/1‑149 (MQ=255)
                                                                                                                                        tGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTc        <  1:3402379/149‑1 (MQ=255)
                                                                                                                                         gTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCa       <  2:3870360/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGG  >  CP009273/158141‑158431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑