Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,870,743 C→A 84.0% H272N (CAT→AAT)  yeaN → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,870,7430CA84.0% 597.1 / 134.9 276H272N (CAT→AAT) yeaNputative transporter
Reads supporting (aligned to +/- strand):  ref base C (28/16);  new base A (127/104);  total (155/121)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

Only 100 of 282 total aligned reads displayed.

ACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTT  >  CP009273/1870597‑1870888
                                                                                                                                                  |                                                                                                                                                 
aCTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTAcataat                                                                                                                                                 <  2:1222668/149‑1 (MQ=255)
   cACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAAtgtg                                                                                                                                              >  1:550814/1‑149 (MQ=255)
    aCTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGa                                                                                                                                             >  1:1059159/1‑149 (MQ=255)
     cTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGaa                                                                                                                                            >  2:3825072/1‑149 (MQ=255)
         tCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGAt                                                                                                                                        <  1:103311/149‑1 (MQ=255)
          cTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATc                                                                                                                                       >  2:4449247/1‑149 (MQ=255)
           tATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCa                                                                                                                                      >  2:4352750/1‑149 (MQ=255)
            aTTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAg                                                                                                                                     >  1:3075660/1‑149 (MQ=255)
             ttACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGc                                                                                                                                    >  1:383407/1‑149 (MQ=255)
             ttACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGc                                                                                                                                    >  1:3474550/1‑149 (MQ=255)
               aCGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGt                                                                                                                                  >  2:3257354/1‑149 (MQ=255)
               aCGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGt                                                                                                                                  <  1:3790652/149‑1 (MQ=255)
                 gTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTgg                                                                                                                                <  2:4469272/149‑1 (MQ=255)
                 gTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTgg                                                                                                                                <  1:2295940/149‑1 (MQ=255)
                  tGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGt                                                                                                                               <  2:240177/149‑1 (MQ=255)
                  tGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGt                                                                                                                               >  1:4410492/1‑149 (MQ=255)
                    attattGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGAGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCACACTTTTCTTACATAATGTGAAAGATCAGCGAGGTAt                                                                                                                             >  2:2530794/1‑149 (MQ=255)
                          ggctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCGCAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGc                                                                                                                       <  1:4784164/149‑1 (MQ=255)
                          ggctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGc                                                                                                                       >  2:3492869/1‑149 (MQ=255)
                            ctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgt                                                                                                                     <  1:89730/149‑1 (MQ=255)
                                 ttCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgttcgtt                                                                                                                >  2:3162833/1‑149 (MQ=255)
                                 ttCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgttcgtt                                                                                                                <  1:1791577/149‑1 (MQ=255)
                                   ccGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGc                                                                                                              <  2:4626131/149‑1 (MQ=255)
                                     ggCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCCTGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCt                                                                                                            <  2:2622325/149‑1 (MQ=255)
                                     ggCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCt                                                                                                            >  1:4649741/1‑149 (MQ=255)
                                     ggCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCt                                                                                                            <  2:3171022/149‑1 (MQ=255)
                                       cGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTg                                                                                                          <  2:141753/149‑1 (MQ=255)
                                       cGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTg                                                                                                          <  2:1329693/149‑1 (MQ=255)
                                          tCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAtg                                                                                                       >  2:3575166/1‑149 (MQ=255)
                                           ccTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAtgt                                                                                                      <  1:3046010/149‑1 (MQ=255)
                                           ccTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAtgt                                                                                                      >  2:51737/1‑149 (MQ=255)
                                           ccTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAtgt                                                                                                      >  1:4235748/1‑149 (MQ=255)
                                            cTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAtgtg                                                                                                     <  2:218000/149‑1 (MQ=255)
                                             tcatcaGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTgc                                                                                                    >  1:534981/1‑149 (MQ=255)
                                                    tCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTgg                                                                                             >  1:2907556/1‑149 (MQ=255)
                                                     cACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTggg                                                                                            <  1:2008348/149‑1 (MQ=255)
                                                         gCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGcgcg                                                                                        >  1:1198384/1‑149 (MQ=255)
                                                           tataGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGt                                                                                      <  2:2692172/149‑1 (MQ=255)
                                                           tataGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGt                                                                                      >  1:102027/1‑149 (MQ=255)
                                                             taGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTg                                                                                    <  2:7756/149‑1 (MQ=255)
                                                             taGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTg                                                                                    <  1:2484292/149‑1 (MQ=255)
                                                              aGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTgg                                                                                   >  1:2619172/1‑149 (MQ=255)
                                                                 gAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGct                                                                                >  2:2320435/1‑149 (MQ=255)
                                                                  aaGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGctc                                                                               <  1:3644762/149‑1 (MQ=255)
                                                                     cacaGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGc                                                                            >  1:4617768/1‑149 (MQ=255)
                                                                     cacaGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGc                                                                            <  2:2647201/149‑1 (MQ=255)
                                                                     cacaGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGc                                                                            <  1:3491702/149‑1 (MQ=255)
                                                                      acaGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCt                                                                           <  1:1945196/149‑1 (MQ=255)
                                                                       caGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCtt                                                                          <  2:1488491/149‑1 (MQ=255)
                                                                        aGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCttt                                                                         <  2:1001079/149‑1 (MQ=255)
                                                                            gggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGc                                                                     >  2:4098623/1‑149 (MQ=255)
                                                                            gggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGc                                                                     <  2:3355102/149‑1 (MQ=255)
                                                                            gggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGc                                                                     <  2:3905566/149‑1 (MQ=255)
                                                                             ggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCTCAGTTGGCGCGGTTGGGCTCTGATTTATGcc                                                                    >  2:3313839/1‑149 (MQ=255)
                                                                             ggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGcc                                                                    <  2:2115894/149‑1 (MQ=255)
                                                                               ttCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAg                                                                  >  2:420604/1‑149 (MQ=255)
                                                                                  aCTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAgcgc                                                               >  1:1652012/1‑149 (MQ=255)
                                                                                  aCTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAgcgc                                                               >  1:4199607/1‑149 (MQ=255)
                                                                                     gCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCAcg                                                            >  2:3527432/1‑149 (MQ=255)
                                                                                       aTGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCAcgcg                                                          <  2:2891591/149‑1 (MQ=255)
                                                                                              gctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCt                                                   <  1:1840693/149‑1 (MQ=255)
                                                                                                tgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTgg                                                 <  1:3955031/149‑1 (MQ=255)
                                                                                                 gcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGa                                                >  2:836820/1‑149 (MQ=255)
                                                                                                  cAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGAc                                               <  1:3885141/149‑1 (MQ=255)
                                                                                                    aCTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGActc                                             >  2:2036408/1‑149 (MQ=255)
                                                                                                        gCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCt                                         <  1:1110455/149‑1 (MQ=255)
                                                                                                          cacaGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCttt                                       <  2:65832/149‑1 (MQ=255)
                                                                                                           acaGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCtttt                                      <  1:1449159/149‑1 (MQ=255)
                                                                                                           acaGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCtttt                                      <  1:1449468/149‑1 (MQ=255)
                                                                                                            cagcagCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTc                                     >  2:1502283/1‑149 (MQ=255)
                                                                                                            cagcagCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTc                                     <  1:2981807/149‑1 (MQ=255)
                                                                                                              gcagcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCgg                                   >  1:2720663/1‑149 (MQ=255)
                                                                                                              gcagcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCgg                                   >  1:78588/1‑149 (MQ=255)
                                                                                                               cagcaCCCGGTGTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGt                                  <  1:2016983/149‑1 (MQ=255)
                                                                                                                 gcaCCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGttt                                >  2:1105839/1‑149 (MQ=255)
                                                                                                                 gcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGttt                                >  2:4171205/1‑149 (MQ=255)
                                                                                                                  caCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGtttt                               <  1:2230469/149‑1 (MQ=255)
                                                                                                                  caCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCCGCTTTTCGGtttt                               >  1:668783/1‑149 (MQ=255)
                                                                                                                   aCCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTg                              >  2:1762124/1‑149 (MQ=255)
                                                                                                                     ccGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGt                            <  1:3208364/149‑1 (MQ=255)
                                                                                                                     ccGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGt                            >  2:3635382/1‑149 (MQ=255)
                                                                                                                      cGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGtt                           >  2:2525564/1‑149 (MQ=255)
                                                                                                                      cGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGtt                           >  1:1969095/1‑149 (MQ=255)
                                                                                                                       ggTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTc                          >  1:241892/1‑149 (MQ=255)
                                                                                                                       ggTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTc                          >  2:287425/1‑149 (MQ=255)
                                                                                                                        gTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTcc                         >  2:3412423/1‑149 (MQ=255)
                                                                                                                         tttGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGTCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCg                        >  1:2171415/1‑149 (MQ=255)
                                                                                                                            gCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGgcg                     <  2:2994582/149‑1 (MQ=255)
                                                                                                                              tGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGca                   >  2:4642508/1‑149 (MQ=255)
                                                                                                                                aTCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaac                 >  1:3340398/1‑149 (MQ=255)
                                                                                                                                aTCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaac                 <  2:2557007/149‑1 (MQ=255)
                                                                                                                                aTCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTGTCGGTTTTGGTTCCGGCGcaac                 >  2:4300595/1‑149 (MQ=255)
                                                                                                                                 tCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaaca                >  1:3686333/1‑149 (MQ=255)
                                                                                                                                  cccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaacaa               >  1:3867722/1‑149 (MQ=255)
                                                                                                                                   ccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAAt              <  1:4532221/149‑1 (MQ=255)
                                                                                                                                     aCTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGa            >  1:4257560/1‑149 (MQ=255)
                                                                                                                                      cTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGAt           >  1:740197/1‑149 (MQ=255)
                                                                                                                                           cTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCACACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTgg      >  1:3990828/1‑149 (MQ=255)
                                                                                                                                            ttACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTggg     >  2:1253845/1‑149 (MQ=255)
                                                                                                                                               cataatGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGtt  <  2:4017186/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTT  >  CP009273/1870597‑1870888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑