Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,872,054 A→T 88.8% E28V (GAG→GTG)  yeaP → diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,872,0540AT88.8% 805.5 / 74.8 264E28V (GAG→GTG) yeaPdiguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (19/11);  new base T (135/99);  total (154/110)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 268 total aligned reads displayed.

GATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTA  >  CP009273/1871906‑1872199
                                                                                                                                                    |                                                                                                                                                 
gatgTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGga                                                                                                                                                   <  2:1365065/149‑1 (MQ=255)
 atgTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGgag                                                                                                                                                  >  1:674941/1‑149 (MQ=255)
     tttATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGc                                                                                                                                              <  2:3599305/149‑1 (MQ=255)
     tttATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGc                                                                                                                                              <  2:4491446/149‑1 (MQ=255)
      ttATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCt                                                                                                                                             >  2:22731/1‑149 (MQ=255)
      ttATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCt                                                                                                                                             >  1:3201625/1‑149 (MQ=255)
       tATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTg                                                                                                                                            >  2:255388/1‑149 (MQ=255)
         tCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGa                                                                                                                                          >  2:750584/1‑149 (MQ=255)
            tGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCAGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGACAt                                                                                                                                       >  1:2854000/1‑149 (MQ=255)
                tGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCCATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTc                                                                                                                                   >  2:2831843/1‑149 (MQ=255)
                 gCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCa                                                                                                                                  >  1:3356322/1‑149 (MQ=255)
                    aaGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTg                                                                                                                               >  1:2056371/1‑149 (MQ=255)
                     aGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGa                                                                                                                              >  1:359446/1‑149 (MQ=255)
                      gATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGat                                                                                                                             >  1:1704851/1‑149 (MQ=255)
                             taatCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAAt                                                                                                                      <  2:1381793/149‑1 (MQ=255)
                              aatCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATc                                                                                                                     >  1:2101741/1‑149 (MQ=255)
                               atCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCa                                                                                                                    >  2:680497/1‑149 (MQ=255)
                                 cAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAac                                                                                                                  >  1:951565/1‑149 (MQ=255)
                                   gCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacct                                                                                                                >  2:1953373/1‑149 (MQ=255)
                                   gCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAacct                                                                                                                >  1:4007470/1‑149 (MQ=255)
                                      tttACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGACTCAacctacc                                                                                                             >  2:1687280/1‑149 (MQ=255)
                                      tttACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacctacc                                                                                                             >  1:2657740/1‑149 (MQ=255)
                                          cccGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGAc                                                                                                         >  2:3953330/1‑149 (MQ=255)
                                             gccgTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCaa                                                                                                      >  2:1241170/1‑149 (MQ=255)
                                              ccgTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCaaa                                                                                                     >  1:4587620/1‑149 (MQ=255)
                                               cgTATCCTGTAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAg                                                                                                    <  2:4733855/149‑1 (MQ=255)
                                                  aTCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTgg                                                                                                 >  1:2379106/1‑149 (MQ=255)
                                                  aTCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTgg                                                                                                 >  1:2886768/1‑149 (MQ=255)
                                                      tGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGt                                                                                             >  2:3675822/1‑149 (MQ=255)
                                                       ggAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTc                                                                                            <  2:3469914/149‑1 (MQ=255)
                                                       ggAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTc                                                                                            >  1:1234307/1‑149 (MQ=255)
                                                        gAGTTGTTCCGTGACAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCg                                                                                           >  2:638779/1‑149 (MQ=255)
                                                           ttgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAg                                                                                        <  1:2212374/149‑1 (MQ=255)
                                                           ttgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAg                                                                                        <  2:2987554/149‑1 (MQ=255)
                                                            tgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgc                                                                                       <  2:4631460/149‑1 (MQ=255)
                                                              ttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgc                                                                                     >  2:3155230/1‑149 (MQ=255)
                                                              ttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgc                                                                                     >  1:852851/1‑149 (MQ=255)
                                                              ttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgc                                                                                     >  1:1906655/1‑149 (MQ=255)
                                                              ttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGATGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgc                                                                                     >  1:237523/1‑149 (MQ=255)
                                                               tCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgcg                                                                                    >  1:986203/1‑149 (MQ=255)
                                                                 cGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGcc                                                                                  <  1:4322661/149‑1 (MQ=255)
                                                                  gtgtCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCt                                                                                 >  2:1593018/1‑149 (MQ=255)
                                                                       agatcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgcagc                                                                            <  1:3712635/149‑1 (MQ=255)
                                                                       agatcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgcagc                                                                            >  1:3759523/1‑149 (MQ=255)
                                                                       agatcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgcagc                                                                            >  1:1370960/1‑149 (MQ=255)
                                                                          tcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCata                                                                         >  1:3215073/1‑149 (MQ=255)
                                                                           cagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCatat                                                                        <  2:1819413/149‑1 (MQ=255)
                                                                           cagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCatat                                                                        <  2:1819386/149‑1 (MQ=255)
                                                                              atTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtattat                                                                     >  2:1698239/1‑149 (MQ=255)
                                                                               ttATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATg                                                                    >  1:1542965/1‑149 (MQ=255)
                                                                                tATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGt                                                                   <  1:4374588/149‑1 (MQ=255)
                                                                                tATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGt                                                                   >  1:1871898/1‑149 (MQ=255)
                                                                                 aTCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGtt                                                                  <  1:1415429/149‑1 (MQ=255)
                                                                                 aTCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGtt                                                                  <  1:4192885/149‑1 (MQ=255)
                                                                                  tCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGttt                                                                 <  1:48904/149‑1 (MQ=255)
                                                                                  tCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGttt                                                                 >  1:1683594/1‑149 (MQ=255)
                                                                                  tCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGttt                                                                 >  1:2561652/1‑149 (MQ=255)
                                                                                        gcgTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGt                                                           >  2:849564/1‑149 (MQ=255)
                                                                                          gTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTaa                                                         >  2:1833183/1‑149 (MQ=255)
                                                                                          gTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTaa                                                         <  2:2018417/149‑1 (MQ=255)
                                                                                          gTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTaa                                                         <  1:1317311/149‑1 (MQ=255)
                                                                                             tcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAg                                                      >  1:3541764/1‑149 (MQ=255)
                                                                                             tcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAg                                                      >  1:1332886/1‑149 (MQ=255)
                                                                                               gCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGcc                                                    >  2:2471803/1‑149 (MQ=255)
                                                                                                cAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCa                                                   >  1:4530984/1‑149 (MQ=255)
                                                                                                  aTCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGa                                                 >  2:3941364/1‑149 (MQ=255)
                                                                                                   tCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaa                                                >  1:3522705/1‑149 (MQ=255)
                                                                                                     ccTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaaaa                                              >  1:2251964/1‑149 (MQ=255)
                                                                                                     ccTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaaaa                                              <  2:2741228/149‑1 (MQ=255)
                                                                                                      cTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAAt                                             >  1:3618974/1‑149 (MQ=255)
                                                                                                       ttGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATg                                            >  1:2710098/1‑149 (MQ=255)
                                                                                                          ccAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTAc                                         <  1:300133/149‑1 (MQ=255)
                                                                                                             aaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                      >  1:3024287/1‑149 (MQ=255)
                                                                                                             aaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                      >  2:509180/1‑149 (MQ=255)
                                                                                                             aaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                      <  1:1815485/149‑1 (MQ=255)
                                                                                                             aaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                      <  1:4457525/149‑1 (MQ=255)
                                                                                                               gAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATccc                                    <  1:404631/149‑1 (MQ=255)
                                                                                                               gAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATccc                                    >  1:713280/1‑149 (MQ=255)
                                                                                                                   aGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGgag                                >  1:4687299/1‑149 (MQ=255)
                                                                                                                     tCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGaa                              <  1:2768151/149‑1 (MQ=255)
                                                                                                                      cACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAt                             <  1:177686/149‑1 (MQ=255)
                                                                                                                        cTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAttt                           <  1:2303854/149‑1 (MQ=255)
                                                                                                                         tGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAtttt                          <  2:3983126/149‑1 (MQ=255)
                                                                                                                          ggAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTa                         >  2:2865221/1‑149 (MQ=255)
                                                                                                                          ggAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTa                         >  2:160708/1‑149 (MQ=255)
                                                                                                                           gAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTAc                        <  1:22731/149‑1 (MQ=255)
                                                                                                                                 cTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGtctc                  <  1:285779/149‑1 (MQ=255)
                                                                                                                                    gTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTg               <  1:781888/149‑1 (MQ=255)
                                                                                                                                    gTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTg               <  1:1181153/149‑1 (MQ=255)
                                                                                                                                     tCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTgg              >  1:1991865/1‑149 (MQ=255)
                                                                                                                                        gACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAt           <  2:3069357/149‑1 (MQ=255)
                                                                                                                                         aCAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAtt          >  2:4554292/1‑149 (MQ=255)
                                                                                                                                           aGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTAc        >  1:2960640/1‑149 (MQ=255)
                                                                                                                                            gCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACt       >  1:279126/1‑149 (MQ=255)
                                                                                                                                            gCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACt       >  2:3744063/1‑149 (MQ=255)
                                                                                                                                             cTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTc      <  2:3205944/149‑1 (MQ=255)
                                                                                                                                               tCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGt    <  1:3675822/149‑1 (MQ=255)
                                                                                                                                               tCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGt    <  1:1072227/149‑1 (MQ=255)
                                                                                                                                               tCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGt    <  2:4699454/149‑1 (MQ=255)
                                                                                                                                                cTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGtt   <  1:561282/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTA  >  CP009273/1871906‑1872199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑