BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,913,162	G→T	9.9%	V466F (GTT→TTT)	yebT →	MCE domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,913,162	0	G	T	9.9%	982.4 / 52.3	277	V466F (GTT→TTT)	yebT	MCE domain protein
Reads supporting (aligned to +/- strand): ref base G (119/130); new base T (12/16); total (131/146)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

CAGGTAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCAC  >  CP009273/1913015‑1913310

cAGGTAGGCCAGTTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAATGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGt                                                                                                                                                     <  1:1111483/149‑1 (MQ=255)

       gCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGttt                                                                                                                                              <  2:1141216/149‑1 (MQ=255)

        ccAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTtc                                                                                                                                             <  2:411752/149‑1 (MQ=255)

         cAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTtct                                                                                                                                            <  1:1991883/149‑1 (MQ=255)

         cAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTtct                                                                                                                                            <  2:3388088/149‑1 (MQ=255)

              tATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTg                                                                                                                                       >  1:2422579/1‑149 (MQ=255)

                tcgatcgTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGcc                                                                                                                                     >  1:1144705/1‑149 (MQ=255)

                tcgatcgTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGcc                                                                                                                                     >  1:4404537/1‑149 (MQ=255)

                  gatcgTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAg                                                                                                                                   <  1:1416898/149‑1 (MQ=255)

                      gTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGcc                                                                                                                               <  2:4339438/149‑1 (MQ=255)

                      gTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGcc                                                                                                                               <  2:4339410/149‑1 (MQ=255)

                       tAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCt                                                                                                                              >  1:73390/1‑149 (MQ=255)

                         aaCTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCa                                                                                                                            >  2:3740448/1‑149 (MQ=255)

                         aaCTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCa                                                                                                                            <  1:4333578/149‑1 (MQ=255)

                            tCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGaa                                                                                                                         <  1:670283/149‑1 (MQ=255)

                             cACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGact                                                                                                                        >  2:2453153/1‑147 (MQ=255)

                             cACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAAt                                                                                                                        <  1:2125442/149‑1 (MQ=255)

                             cACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAAt                                                                                                                        >  1:3169625/1‑149 (MQ=255)

                              aCCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATg                                                                                                                       >  2:3005156/1‑149 (MQ=255)

                                cAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGa                                                                                                                     <  1:3892077/149‑1 (MQ=255)

                                 aGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGAt                                                                                                                    >  2:4159105/1‑149 (MQ=255)

                                    aaaGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCaa                                                                                                                 <  1:1985095/149‑1 (MQ=255)

                                     aaGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAAt                                                                                                                >  1:222906/1‑149 (MQ=255)

                                      tggCGTCTCCTTTACCGTCGCCATCGAGCCTCAGCATCTCGAACTGGTATAAGGCGATTGCAATTTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGTATGGATGGCGTTTAGTTTCTCGGTTCCAGCTCCTCAGAATGGATCAAtt                                                                                                               <  2:192577/148‑2 (MQ=255)

                                       ggCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATgg                                                                                                              <  1:1386848/149‑1 (MQ=255)

                                          gTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCgg                                                                                                           >  2:4236166/1‑149 (MQ=255)

                                             aCCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGAt                                                                                                        >  2:3882939/1‑149 (MQ=255)

                                             aCCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGAt                                                                                                        <  1:2467521/149‑1 (MQ=255)

                                              ccTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATa                                                                                                       <  1:860969/149‑1 (MQ=255)

                                               cTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATAc                                                                                                      <  2:2967952/149‑1 (MQ=255)

                                                tttACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACg                                                                                                     >  1:3518494/1‑149 (MQ=255)

                                                  tACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTa                                                                                                   >  1:3262746/1‑149 (MQ=255)

                                                   aCCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTAt                                                                                                  <  2:2725508/149‑1 (MQ=255)

                                                    ccGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTAtt                                                                                                 <  1:2737071/149‑1 (MQ=255)

                                                    ccGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTAtt                                                                                                 <  2:3415106/149‑1 (MQ=255)

                                                             tCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGc                                                                                        <  1:3074784/149‑1 (MQ=255)

                                                             tCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGc                                                                                        >  2:4678288/1‑149 (MQ=255)

                                                             tCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGc                                                                                        >  1:1471585/1‑149 (MQ=255)

                                                               gAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGa                                                                                      <  1:3577421/149‑1 (MQ=255)

                                                                 gCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATa                                                                                    >  1:2877989/1‑149 (MQ=255)

                                                                 gCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATa                                                                                    <  1:2840232/149‑1 (MQ=255)

                                                                   cTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATaaa                                                                                  <  1:3798112/149‑1 (MQ=255)

                                                                   cTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATaaa                                                                                  >  1:2082098/1‑149 (MQ=255)

                                                                   cTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATaaa                                                                                  <  1:701166/149‑1 (MQ=255)

                                                                     cAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAgg                                                                                <  2:2230638/149‑1 (MQ=255)

                                                                       gCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTg                                                                              >  2:3510879/1‑149 (MQ=255)

                                                                          tCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTgaga                                                                           <  1:1564691/149‑1 (MQ=255)

                                                                            gcgAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATg                                                                         <  2:63496/149‑1 (MQ=255)

                                                                            gcgAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATg                                                                         <  2:1229296/149‑1 (MQ=255)

                                                                              gAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGaa                                                                       >  1:2194226/1‑149 (MQ=255)

                                                                                aCTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAg                                                                     <  1:3802509/149‑1 (MQ=255)

                                                                                aCTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAg                                                                     <  2:4390828/149‑1 (MQ=255)

                                                                                 cTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGc                                                                    <  2:89480/149‑1 (MQ=255)

                                                                                   ggTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCa                                                                  <  2:1082508/149‑1 (MQ=255)

                                                                                      aaaaGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCt                                                               >  1:603648/1‑149 (MQ=255)

                                                                                      aaaaGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCt                                                               >  2:3922391/1‑149 (MQ=255)

                                                                                      aaaaGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCt                                                               >  2:4323387/1‑149 (MQ=255)

                                                                                         aGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATc                                                            <  1:4606302/149‑1 (MQ=255)

                                                                                         aGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATc                                                            >  1:4423451/1‑149 (MQ=255)

                                                                                           gCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCa                                                          >  2:1326006/1‑149 (MQ=255)

                                                                                              aTAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg                                                       >  2:4709896/1‑149 (MQ=255)

                                                                                               tAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGt                                                      >  1:4788253/1‑149 (MQ=255)

                                                                                                   aaaTTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGc                                                  >  1:1937605/1‑149 (MQ=255)

                                                                                                      tttGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCaa                                               <  1:4456821/149‑1 (MQ=255)

                                                                                                      tttGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCaa                                               >  2:1864628/1‑149 (MQ=255)

                                                                                                        tGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAAtt                                             <  1:2611046/149‑1 (MQ=255)

                                                                                                         gtcgtcAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAAttt                                            <  2:3687830/149‑1 (MQ=255)

                                                                                                         gtcgtcAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAAttt                                            >  1:2570583/1‑149 (MQ=255)

                                                                                                         gtcgtcAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAAttt                                            >  2:3070264/1‑149 (MQ=255)

                                                                                                         gtcgtcAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAAttt                                            <  1:1720487/149‑1 (MQ=255)

                                                                                                         gtagtcAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGTCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAATGTGAGATGAAAGCCAGCTATCCACTGTATGCCAAttt                                            <  1:3868815/146‑1 (MQ=255)

                                                                                                          tcgtcAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTg                                           >  2:2256464/1‑149 (MQ=255)

                                                                                                          tcgtcAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTg                                           <  2:2339594/149‑1 (MQ=255)

                                                                                                           cgtcAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTgg                                          <  1:2945855/149‑1 (MQ=255)

                                                                                                             tcAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGaa                                        >  2:2613611/1‑149 (MQ=255)

                                                                                                               aaCAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGaaaa                                      >  1:4044319/1‑149 (MQ=255)

                                                                                                               aaCAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGaaaa                                      >  1:2990403/1‑149 (MQ=255)

                                                                                                                 cAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAg                                    >  2:2032924/1‑149 (MQ=255)

                                                                                                                 cAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAg                                    <  2:3223305/149‑1 (MQ=255)

                                                                                                                 cAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAg                                    <  2:1582054/149‑1 (MQ=255)

                                                                                                                      gtgtCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTg                               >  1:3443432/1‑149 (MQ=255)

                                                                                                                       tgtCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTgg                              >  2:4458800/1‑149 (MQ=255)

                                                                                                                       tgtCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTgg                              <  2:1406462/149‑1 (MQ=255)

                                                                                                                           gATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGaa                          <  1:1274070/149‑1 (MQ=255)

                                                                                                                            aTGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc                         >  2:2534432/1‑149 (MQ=255)

                                                                                                                             tgtgAAGGTGGGGCTGGATGGCTTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACa                        <  2:758810/149‑1 (MQ=255)

                                                                                                                             tgtgAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACa                        >  2:103390/1‑149 (MQ=255)

                                                                                                                             tgtgAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACa                        <  2:2294685/149‑1 (MQ=255)

                                                                                                                              gtgAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAg                       <  1:1873927/149‑1 (MQ=255)

                                                                                                                              gtgAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAg                       <  1:4731691/149‑1 (MQ=255)

                                                                                                                                   ggTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTa                  <  1:234890/149‑1 (MQ=255)

                                                                                                                                   ggTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTa                  <  1:1019889/149‑1 (MQ=255)

                                                                                                                                    gTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAg                 <  2:4093535/149‑1 (MQ=255)

                                                                                                                                       gggCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGa              <  1:3420324/149‑1 (MQ=255)

                                                                                                                                           tGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTa          >  1:3727239/1‑149 (MQ=255)

                                                                                                                                              aTGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCGGGAGAACAGCCTTAGCGATTTAccc       <  1:4198462/149‑1 (MQ=255)

                                                                                                                                                ggCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACccac     <  1:76266/149‑1 (MQ=255)

                                                                                                                                                ggCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACccac     >  1:4148624/1‑149 (MQ=255)

                                                                                                                                                   gTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCcaccac  >  2:484847/1‑149 (MQ=255)

                                                                                                                                                   gTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCcaccac  >  2:3611479/1‑149 (MQ=255)

CAGGTAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCAC  >  CP009273/1913015‑1913310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`