Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,935,501 C→A 23.8% R131L (CGT→CTT)  mepM ← murein DD‑endopeptidase, space‑maker hydrolase, septation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,935,5010CA23.8% 663.3 / 160.3 273R131L (CGT→CTT) mepMmurein DD‑endopeptidase, space‑maker hydrolase, septation protein
Reads supporting (aligned to +/- strand):  ref base C (79/129);  new base A (29/36);  total (108/165)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 277 total aligned reads displayed.

TTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTG  >  CP009273/1935353‑1935649
                                                                                                                                                    |                                                                                                                                                    
ttGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAc                                                                                                                                                      <  1:1238219/149‑1 (MQ=255)
ttGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAc                                                                                                                                                      >  2:2692518/1‑149 (MQ=255)
  gctgcATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGc                                                                                                                                                    <  1:3565789/149‑1 (MQ=255)
  gctgcATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGc                                                                                                                                                    <  1:3566204/149‑1 (MQ=255)
      cATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAAtt                                                                                                                                                >  2:3313612/1‑149 (MQ=255)
       aTTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTc                                                                                                                                               >  1:3485247/1‑149 (MQ=255)
         ttCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCtt                                                                                                                                             <  1:1861980/149‑1 (MQ=255)
          tCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCttt                                                                                                                                            >  1:1808752/1‑149 (MQ=255)
          tCGCTGGTAATTTTAAAACCGTTAGCGGCAGTACTGTAATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGTCGCTTCAGTTCTCAGTCCGAGGTTAATGTCCAGGATAGTTGTTGACCGATTTTCAGGTTACGCAATTCttt                                                                                                                                            <  1:3054920/149‑1 (MQ=255)
           cGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTg                                                                                                                                           <  1:3236957/149‑1 (MQ=255)
           cGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTg                                                                                                                                           >  2:1739719/1‑149 (MQ=255)
                gTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGc                                                                                                                                      <  1:1005254/149‑1 (MQ=255)
                 tCATTTTAAAACCGTTAGCGGCAGTACGGTCATCGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGcc                                                                                                                                     <  2:4137962/149‑1 (MQ=255)
                 tCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGcc                                                                                                                                     >  2:3863558/1‑149 (MQ=255)
                 tCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGcc                                                                                                                                     >  1:3719400/1‑149 (MQ=255)
                   aTTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACGCTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGc                                                                                                                                   >  1:2016115/1‑149 (MQ=255)
                   aTTTTAAAACCGTTAGCGGCAGAACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGc                                                                                                                                   >  2:3353476/1‑149 (MQ=255)
                    ttttAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCTGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCa                                                                                                                                  >  2:3424418/1‑149 (MQ=255)
                    ttttAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCa                                                                                                                                  >  2:754994/1‑149 (MQ=255)
                    ttttAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCa                                                                                                                                  >  1:775840/1‑149 (MQ=255)
                        aaaaCCGTTAGCTGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACATCACAGGTGAGTCGCTGAAGTTCGCCGTCCTCGGGTAATGTCCAGGAGAGTTGTTTACTGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCa                                                                                                                              >  2:1832869/1‑149 (MQ=255)
                        aaaaCCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCa                                                                                                                              >  1:1389096/1‑149 (MQ=255)
                        aaaaCCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCa                                                                                                                              >  2:1387532/1‑149 (MQ=255)
                          aaCCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCCGGTGAGGCGCTGCAGGTCGCCGTCCGCGGTTAAGGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCACTTCTTTGTCGGCCGCAGCCAGt                                                                                                                            <  2:1789151/149‑1 (MQ=255)
                          aaCCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGt                                                                                                                            <  2:3218221/149‑1 (MQ=255)
                          aaCCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGt                                                                                                                            <  2:2769287/149‑1 (MQ=255)
                           aCCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGtt                                                                                                                           <  1:691543/149‑1 (MQ=255)
                             cGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTgg                                                                                                                         <  2:2021819/149‑1 (MQ=255)
                              gtgaGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTggg                                                                                                                        <  2:2508433/146‑1 (MQ=255)
                              gTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTggg                                                                                                                        <  1:4747395/149‑1 (MQ=255)
                                tAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTg                                                                                                                      <  1:546649/149‑1 (MQ=255)
                                tAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTg                                                                                                                      <  1:2940103/149‑1 (MQ=255)
                                 aGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGa                                                                                                                     >  1:4122793/1‑149 (MQ=255)
                                  gCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGat                                                                                                                    <  2:2372648/149‑1 (MQ=255)
                                   cGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGata                                                                                                                   <  2:4782516/149‑1 (MQ=255)
                                     gCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGATGGGTGATATc                                                                                                                 >  1:4695257/1‑149 (MQ=255)
                                           cGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCat                                                                                                           <  2:4765027/149‑1 (MQ=255)
                                            ggTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCata                                                                                                          >  2:1553047/1‑149 (MQ=255)
                                            ggTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCata                                                                                                          <  2:4167512/149‑1 (MQ=255)
                                            ggTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCata                                                                                                          >  1:137480/1‑149 (MQ=255)
                                             gTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCatat                                                                                                         <  2:2129020/149‑1 (MQ=255)
                                              tCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTAGGGTGATATCACCCATATc                                                                                                        >  1:1359944/1‑149 (MQ=255)
                                                aTAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCaa                                                                                                      <  1:3390956/149‑1 (MQ=255)
                                                  aGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATa                                                                                                    <  1:3954065/149‑1 (MQ=255)
                                                  aGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATa                                                                                                    <  2:1944391/149‑1 (MQ=255)
                                                   ggTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATAc                                                                                                   >  1:2655458/1‑149 (MQ=255)
                                                   ggTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATAc                                                                                                   >  1:901147/1‑149 (MQ=255)
                                                       cGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATa                                                                                               <  1:4331896/149‑1 (MQ=255)
                                                       cGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATa                                                                                               <  2:4282029/149‑1 (MQ=255)
                                                        gggTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATAc                                                                                              <  1:3117618/149‑1 (MQ=255)
                                                         ggTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACt                                                                                             <  2:3001458/149‑1 (MQ=255)
                                                          gTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTg                                                                                            <  1:2402800/149‑1 (MQ=255)
                                                           tttCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGa                                                                                           <  2:2837933/149‑1 (MQ=255)
                                                                  acgaGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGaa                                                                                    <  2:1320484/149‑1 (MQ=255)
                                                                  acgaGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGaa                                                                                    >  1:4052678/1‑149 (MQ=255)
                                                                   cgaGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAAt                                                                                   <  2:4034993/149‑1 (MQ=255)
                                                                     agaCACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAAtgc                                                                                 >  1:1319445/1‑149 (MQ=255)
                                                                     agaCACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAAtgc                                                                                 <  2:85769/149‑1 (MQ=255)
                                                                     agaCACTTCACAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAAtgc                                                                                 <  2:293424/149‑1 (MQ=255)
                                                                        cacTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAAtgctgc                                                                              >  2:3642919/1‑149 (MQ=255)
                                                                         acTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATgctgct                                                                             <  2:3119421/149‑1 (MQ=255)
                                                                           ttCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTa                                                                           <  2:1808644/149‑1 (MQ=255)
                                                                            tCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAg                                                                          <  1:2609880/149‑1 (MQ=255)
                                                                            tCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAg                                                                          >  1:175805/1‑149 (MQ=255)
                                                                             cccAGGTGAGGCGCTGCAGGTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTTTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGc                                                                         <  1:2459658/149‑1 (MQ=255)
                                                                                     aGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGcc                                                                 <  2:1714537/149‑1 (MQ=255)
                                                                                       gcgcTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAg                                                               >  2:3675990/1‑149 (MQ=255)
                                                                                       gcgcTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAg                                                               <  2:1055850/149‑1 (MQ=255)
                                                                                       gcgcTGCAGGTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAg                                                               <  2:3617757/149‑1 (MQ=255)
                                                                                        cgcTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGt                                                              <  1:2870286/149‑1 (MQ=255)
                                                                                        cgcTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGt                                                              <  2:3958620/149‑1 (MQ=255)
                                                                                           tGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGa                                                           >  2:265868/1‑149 (MQ=255)
                                                                                           tGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGa                                                           >  1:721427/1‑149 (MQ=255)
                                                                                               gTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAaaca                                                       >  1:3003391/1‑149 (MQ=255)
                                                                                                   gCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACat                                                   <  2:313816/149‑1 (MQ=255)
                                                                                                     cGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACatat                                                 <  2:939591/149‑1 (MQ=255)
                                                                                                     cGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACatat                                                 <  1:2511317/149‑1 (MQ=255)
                                                                                                       tCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTc                                               >  1:1510919/1‑149 (MQ=255)
                                                                                                        ccGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCa                                              <  2:4354398/149‑1 (MQ=255)
                                                                                                         cgcgGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCAt                                             <  1:4515549/149‑1 (MQ=255)
                                                                                                          gcgGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATg                                            <  1:2201783/149‑1 (MQ=255)
                                                                                                                aaTGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTTCTTAGAGTATCGCCAGTGGAAACAACATATTCATTCACGcc                                      >  2:2726256/1‑149 (MQ=255)
                                                                                                                      cAGGAGAGTTGTTGACCGATTTTCAGGTTTCGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTc                                <  1:3842499/149‑1 (MQ=255)
                                                                                                                      cAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTc                                >  1:3508307/1‑149 (MQ=255)
                                                                                                                       aGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCa                               >  1:585298/1‑149 (MQ=255)
                                                                                                                        ggAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCAc                              <  1:3512007/149‑1 (MQ=255)
                                                                                                                          agagTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCg                            <  1:4784786/149‑1 (MQ=255)
                                                                                                                             gttgttGACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCg                         >  2:4375157/1‑149 (MQ=255)
                                                                                                                               tgttgACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGAt                       >  2:1051471/1‑149 (MQ=255)
                                                                                                                                gttgACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGAtt                      <  2:3087132/149‑1 (MQ=255)
                                                                                                                                gttgACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGAtt                      <  2:468303/149‑1 (MQ=255)
                                                                                                                                gttgACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCAGGCACGCCCGCTTCACCGGCGAtt                      <  2:3023188/149‑1 (MQ=255)
                                                                                                                                   gACCGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTg                   <  2:3718863/149‑1 (MQ=255)
                                                                                                                                      cGATTTTCAGGTTAAGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCa                >  2:1222619/1‑149 (MQ=255)
                                                                                                                                         ttttCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATcc             <  2:3352281/149‑1 (MQ=255)
                                                                                                                                             cAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGtt         >  2:4640294/1‑149 (MQ=255)
                                                                                                                                                 ttACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATTCACGCCCTCTTCACCGGCGATTTTGTCATACAGTTctta     >  2:1516531/1‑146 (MQ=255)
                                                                                                                                                 ttACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTc     <  2:2327511/149‑1 (MQ=255)
                                                                                                                                                   aCGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCt   <  1:3083282/149‑1 (MQ=255)
                                                                                                                                                    cGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTg  <  1:2297930/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTG  >  CP009273/1935353‑1935649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑