BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,944,177	C→A	100%	L201L (CTG→CTT)	aspS ←	aspartyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,944,177	0	C	A	100.0%	1052.8 / NA	303	L201L (CTG→CTT)	aspS	aspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (163/140); total (163/140)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 325 total aligned reads displayed.

GCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCAT  >  CP009273/1944031‑1944322

gcTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATaag                                                                                                                                                 >  2:4422468/1‑149 (MQ=255)

     cATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCt                                                                                                                                            <  2:4167406/149‑1 (MQ=255)

       tCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGt                                                                                                                                          <  2:4691354/149‑1 (MQ=255)

            tCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGaa                                                                                                                                     <  1:3967776/149‑1 (MQ=255)

               cGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGaacaa                                                                                                                                  <  2:3075306/149‑1 (MQ=255)

                 cACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACt                                                                                                                                <  1:2289953/149‑1 (MQ=255)

                    ttGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCg                                                                                                                             >  2:328499/1‑149 (MQ=255)

                      gcggcgCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCggg                                                                                                                           <  1:1581757/149‑1 (MQ=255)

                         gcgcGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGAt                                                                                                                        <  2:3813983/149‑1 (MQ=255)

                           gcgGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTg                                                                                                                      <  2:2686535/149‑1 (MQ=255)

                               tCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGc                                                                                                                  <  2:9124/149‑1 (MQ=255)

                                 aTGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAg                                                                                                                >  1:1424068/1‑149 (MQ=255)

                                 aTGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAg                                                                                                                >  1:1515465/1‑149 (MQ=255)

                                  tGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGt                                                                                                               >  2:1444138/1‑149 (MQ=255)

                                  tGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGt                                                                                                               >  1:2130103/1‑149 (MQ=255)

                                   gAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTg                                                                                                              >  1:3568579/1‑149 (MQ=255)

                                    aaaGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACCTCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGc                                                                                                             >  2:3276513/1‑149 (MQ=255)

                                    aaaGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGc                                                                                                             <  2:2928484/149‑1 (MQ=255)

                                     aagaagTTTCCACGTCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCg                                                                                                            >  2:1106046/1‑149 (MQ=255)

                                      agaagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGt                                                                                                           >  2:1171448/1‑149 (MQ=255)

                                      agaagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGt                                                                                                           <  1:1902681/149‑1 (MQ=255)

                                       gaagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTa                                                                                                          >  2:4242421/1‑149 (MQ=255)

                                          gTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGaa                                                                                                       >  1:640484/1‑149 (MQ=255)

                                          gTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGaa                                                                                                       >  1:3834799/1‑149 (MQ=255)

                                          gTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGaa                                                                                                       >  1:3813728/1‑149 (MQ=255)

                                            ttCCACATCGATATGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACTGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGATTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAAtt                                                                                                     <  1:1184205/149‑1 (MQ=255)

                                             tCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAAttt                                                                                                    >  2:1880058/1‑149 (MQ=255)

                                               cacaTCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTAc                                                                                                  >  2:339052/1‑149 (MQ=255)

                                                 caTCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCt                                                                                                <  1:1015959/149‑1 (MQ=255)

                                                   tCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCttt                                                                                              >  2:3451900/1‑149 (MQ=255)

                                                   tCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCttt                                                                                              <  1:1774284/149‑1 (MQ=255)

                                                     gATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgt                                                                                            <  2:2623402/149‑1 (MQ=255)

                                                     gATCTGAGGAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgt                                                                                            <  1:3037020/149‑1 (MQ=255)

                                                      aTCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgtg                                                                                           <  2:2354128/149‑1 (MQ=255)

                                                      aTCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgtg                                                                                           >  1:1167541/1‑149 (MQ=255)

                                                        cTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGca                                                                                         <  2:960397/149‑1 (MQ=255)

                                                         tGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGcac                                                                                        >  1:2469760/1‑149 (MQ=255)

                                                         tGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGcac                                                                                        >  2:1800956/1‑149 (MQ=255)

                                                          gAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGcaca                                                                                       <  2:3213751/149‑1 (MQ=255)

                                                           aGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCacac                                                                                      >  1:4700997/1‑149 (MQ=255)

                                                            gTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACg                                                                                     >  2:1926022/1‑149 (MQ=255)

                                                            gTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACg                                                                                     >  2:1925932/1‑149 (MQ=255)

                                                            gTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACg                                                                                     >  2:722631/1‑149 (MQ=255)

                                                               aaTTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACgaga                                                                                  >  1:2210075/1‑149 (MQ=255)

                                                                aTTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGaa                                                                                 <  2:1031030/149‑1 (MQ=255)

                                                                 ttCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAg                                                                                <  2:4469189/149‑1 (MQ=255)

                                                                 ttCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAg                                                                                <  1:1782946/149‑1 (MQ=255)

                                                                 ttCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAg                                                                                <  2:560840/149‑1 (MQ=255)

                                                                 ttCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAg                                                                                <  2:2070252/149‑1 (MQ=255)

                                                                      gCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCAcc                                                                           <  1:3105399/149‑1 (MQ=255)

                                                                        tGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAg                                                                         >  2:1656928/1‑149 (MQ=255)

                                                                        tGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAg                                                                         >  1:3654098/1‑149 (MQ=255)

                                                                         gACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAgg                                                                        >  1:960578/1‑149 (MQ=255)

                                                                          aCGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGt                                                                       >  1:2795131/1‑149 (MQ=255)

                                                                              tCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTc                                                                   >  1:2294763/1‑149 (MQ=255)

                                                                              tCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTc                                                                   <  2:4612034/149‑1 (MQ=255)

                                                                               cAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCa                                                                  <  1:816477/149‑1 (MQ=255)

                                                                                aGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAc                                                                 >  1:3199755/1‑149 (MQ=255)

                                                                                 gCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAcg                                                                >  2:2099617/1‑149 (MQ=255)

                                                                                       aGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGcc                                                          <  2:3675566/149‑1 (MQ=255)

                                                                                       aGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGcc                                                          >  1:590515/1‑149 (MQ=255)

                                                                                          tCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTc                                                       <  2:3902359/149‑1 (MQ=255)

                                                                                           cTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTcc                                                      >  2:1067252/1‑149 (MQ=255)

                                                                                           cTTCGTCACGGAAGCATTTAACGATCTGATAATAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTcc                                                      <  1:3223433/149‑1 (MQ=255)

                                                                                                tCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGt                                                 >  2:88461/1‑149 (MQ=255)

                                                                                                       aGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTg                                          <  2:2219867/149‑1 (MQ=255)

                                                                                                         cATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGt                                        <  1:3689844/149‑1 (MQ=255)

                                                                                                           tttAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCa                                      >  2:3572901/1‑149 (MQ=255)

                                                                                                           tttAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCa                                      >  1:1978165/1‑149 (MQ=255)

                                                                                                           tttAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCa                                      <  1:3383774/149‑1 (MQ=255)

                                                                                                            ttAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAg                                     >  2:660976/1‑149 (MQ=255)

                                                                                                              aaCGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCa                                   >  2:975024/1‑149 (MQ=255)

                                                                                                                 gATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCg                                >  1:2332601/1‑149 (MQ=255)

                                                                                                                  aTCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCgg                               >  2:2008970/1‑149 (MQ=255)

                                                                                                                   tCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGa                              >  1:4746282/1‑149 (MQ=255)

                                                                                                                     tGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGt                            <  1:3493330/149‑1 (MQ=255)

                                                                                                                     tGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGt                            <  1:4436405/149‑1 (MQ=255)

                                                                                                                      gATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGtt                           >  2:3268225/1‑149 (MQ=255)

                                                                                                                      gATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGtt                           <  1:4040241/149‑1 (MQ=255)

                                                                                                                      gATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGtt                           >  2:3139281/1‑149 (MQ=255)

                                                                                                                       aTAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGttt                          >  1:1124113/1‑149 (MQ=255)

                                                                                                                       aTAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGttt                          <  1:343986/149‑1 (MQ=255)

                                                                                                                        tAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTCGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTc                         >  1:4033734/1‑149 (MQ=255)

                                                                                                                        tAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTc                         >  2:1296033/1‑149 (MQ=255)

                                                                                                                          gTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGa                       >  1:4521692/1‑149 (MQ=255)

                                                                                                                           tAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGAt                      <  2:2360283/149‑1 (MQ=255)

                                                                                                                                gTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGGAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGa                 <  1:655891/149‑1 (MQ=255)

                                                                                                                                  cAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAgg               <  1:4293648/149‑1 (MQ=255)

                                                                                                                                  cAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAgg               <  1:420102/149‑1 (MQ=255)

                                                                                                                                   aaaaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGa              >  2:2534192/1‑149 (MQ=255)

                                                                                                                                    aaaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGaa             >  2:3717846/1‑149 (MQ=255)

                                                                                                                                    aaaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGaa             >  2:1013981/1‑149 (MQ=255)

                                                                                                                                      aCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGc           <  2:3575833/149‑1 (MQ=255)

                                                                                                                                      aCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGc           >  1:513997/1‑149 (MQ=255)

                                                                                                                                        cGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCg         <  2:4034755/149‑1 (MQ=255)

                                                                                                                                              tcataaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCa   >  2:1263501/1‑149 (MQ=255)

                                                                                                                                              tcataaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCa   >  2:4140165/1‑149 (MQ=255)

                                                                                                                                              tcataaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCa   >  1:2611396/1‑149 (MQ=255)

                                                                                                                                              tcataaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCa   <  1:1486398/149‑1 (MQ=255)

                                                                                                                                               cataaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCAt  >  2:4522690/1‑149 (MQ=255)

GCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCAT  >  CP009273/1944031‑1944322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`