Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 173,725 G→T 91.3% G129G (GGC→GGA)  yadS ← UPF0126 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273173,7250GT91.3% 715.2 / 56.1 253G129G (GGC→GGA) yadSUPF0126 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (9/13);  new base T (125/106);  total (134/119)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

ATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGC  >  CP009273/173579‑173869
                                                                                                                                                  |                                                                                                                                                
atcatACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCTCGAATGATCCCgcctcc                                                                                                                                                >  2:966735/1‑149 (MQ=255)
atcatACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCgccgcc                                                                                                                                                >  1:1550525/1‑149 (MQ=255)
 tcatACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCa                                                                                                                                               >  1:1931816/1‑149 (MQ=255)
   atACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAAc                                                                                                                                             >  1:4023129/1‑149 (MQ=255)
         gCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGACCATCACGAATGATCCCGCCTCCAACGCCAGt                                                                                                                                       >  1:3752219/1‑149 (MQ=255)
          cTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTa                                                                                                                                      >  1:1276662/1‑149 (MQ=255)
            gTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAAt                                                                                                                                    <  1:741329/149‑1 (MQ=255)
            gTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAAt                                                                                                                                    <  1:4494696/149‑1 (MQ=255)
               tCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGAc                                                                                                                                 <  2:1616577/149‑1 (MQ=255)
               tCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGAc                                                                                                                                 <  2:4109732/149‑1 (MQ=255)
                 cAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGc                                                                                                                               >  2:1842685/1‑149 (MQ=255)
                 cAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGc                                                                                                                               >  2:2637349/1‑149 (MQ=255)
                   gTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGccc                                                                                                                             <  2:1596576/149‑1 (MQ=255)
                   gTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGccc                                                                                                                             <  1:235942/149‑1 (MQ=255)
                          ggAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACaaa                                                                                                                      >  2:2899228/1‑149 (MQ=255)
                          ggAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGAGTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACaaa                                                                                                                      >  2:4394995/1‑149 (MQ=255)
                          gaaaaaTGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACaaa                                                                                                                      >  1:3983895/3‑149 (MQ=255)
                           gAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAAc                                                                                                                     <  1:2224046/149‑1 (MQ=255)
                            aaaaTGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAAcc                                                                                                                    <  2:4372456/149‑1 (MQ=255)
                              aaTGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACcgc                                                                                                                  <  1:1714455/149‑1 (MQ=255)
                                tgtgTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGa                                                                                                                >  1:971232/1‑149 (MQ=255)
                                 ttgtAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACTTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGAt                                                                                                               <  1:2927150/148‑1 (MQ=255)
                                 gtgtAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGAt                                                                                                               >  2:3486264/1‑149 (MQ=255)
                                  tgtAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGAtt                                                                                                              <  2:2160618/149‑1 (MQ=255)
                                         aGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGAc                                                                                                       >  2:4287229/1‑149 (MQ=255)
                                          gCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGAcc                                                                                                      <  1:1374478/149‑1 (MQ=255)
                                          gCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGAcc                                                                                                      >  1:603842/1‑149 (MQ=255)
                                               aGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACTAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCtt                                                                                                 <  1:3209519/149‑1 (MQ=255)
                                               aGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCtt                                                                                                 <  1:3726808/149‑1 (MQ=255)
                                                gCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTc                                                                                                <  2:2788486/149‑1 (MQ=255)
                                                   tGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGc                                                                                             >  1:3638449/1‑149 (MQ=255)
                                                   tGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGc                                                                                             >  1:486148/1‑149 (MQ=255)
                                                   tGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATAAACGGACCGGCTTCCGc                                                                                             >  2:2922433/1‑149 (MQ=255)
                                                     gACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCAt                                                                                           <  2:3913476/149‑1 (MQ=255)
                                                     gACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCAt                                                                                           <  1:2794600/149‑1 (MQ=255)
                                                      aCAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCAtt                                                                                          <  2:1238120/149‑1 (MQ=255)
                                                      aCAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCAtt                                                                                          >  2:519534/1‑149 (MQ=255)
                                                       cAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTa                                                                                         >  1:3130834/1‑149 (MQ=255)
                                                        aaTACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTaa                                                                                        >  1:111163/1‑149 (MQ=255)
                                                            ccgccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGc                                                                                    <  1:4092932/149‑1 (MQ=255)
                                                             cgccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCt                                                                                   <  1:4658022/149‑1 (MQ=255)
                                                             cgccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCt                                                                                   >  1:4725928/1‑149 (MQ=255)
                                                              gccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCtt                                                                                  >  2:1233048/1‑149 (MQ=255)
                                                              gccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCtt                                                                                  >  2:835458/1‑149 (MQ=255)
                                                               ccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCttt                                                                                 <  2:3972430/149‑1 (MQ=255)
                                                               ccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCttt                                                                                 <  1:680713/149‑1 (MQ=255)
                                                                 gATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAt                                                                               <  2:2328753/149‑1 (MQ=255)
                                                                  ataataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAtt                                                                              >  1:34637/1‑149 (MQ=255)
                                                                   taataCCGGCAGTTGCGTAGATTTCAGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTc                                                                             <  1:4137544/149‑1 (MQ=255)
                                                                    aataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCa                                                                            <  2:2973304/149‑1 (MQ=255)
                                                                    aataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCa                                                                            <  2:581319/149‑1 (MQ=255)
                                                                     ataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCAc                                                                           >  1:52400/1‑149 (MQ=255)
                                                                      taCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACg                                                                          >  2:930862/1‑149 (MQ=255)
                                                                       aCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGc                                                                         <  2:1620747/149‑1 (MQ=255)
                                                                         aGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCa                                                                       >  2:1479661/1‑149 (MQ=255)
                                                                          ggCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCaa                                                                      <  2:98726/149‑1 (MQ=255)
                                                                          ggCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCaa                                                                      <  2:114123/149‑1 (MQ=255)
                                                                           gCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAAt                                                                     <  1:2565893/149‑1 (MQ=255)
                                                                            cAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATg                                                                    <  1:3932025/149‑1 (MQ=255)
                                                                              gTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCCTTAAAGGCTTTATTCACGCCAATGcc                                                                  >  1:1603198/1‑149 (MQ=255)
                                                                              gTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGcc                                                                  >  1:714766/1‑149 (MQ=255)
                                                                                tGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGa                                                                <  1:3588014/149‑1 (MQ=255)
                                                                                 gCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGAc                                                               <  2:1101518/149‑1 (MQ=255)
                                                                                 gCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGAc                                                               <  1:4283446/149‑1 (MQ=255)
                                                                                   gTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACaa                                                             >  2:814736/1‑149 (MQ=255)
                                                                                    tAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACaaa                                                            >  1:910497/1‑149 (MQ=255)
                                                                                    tAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACaaa                                                            >  2:3610731/1‑149 (MQ=255)
                                                                                      gATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                          <  1:1460169/149‑1 (MQ=255)
                                                                                        tttCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACAcc                                                        >  1:2897662/1‑149 (MQ=255)
                                                                                        tttCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACAcc                                                        >  1:3284391/1‑149 (MQ=255)
                                                                                              tACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGa                                                  <  1:2509476/149‑1 (MQ=255)
                                                                                                  tAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCaa                                              <  2:2755575/149‑1 (MQ=255)
                                                                                                  tAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCaa                                              >  1:740181/1‑149 (MQ=255)
                                                                                                     aaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACgg                                           <  2:2547575/149‑1 (MQ=255)
                                                                                                     aaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACgg                                           >  1:3218422/1‑149 (MQ=255)
                                                                                                      aTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGc                                          >  2:3240798/1‑149 (MQ=255)
                                                                                                       tCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCg                                         >  2:775814/1‑149 (MQ=255)
                                                                                                          tGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCTCGCCAATGCCGACAAACACCGCCAGACCAACGGCGTcc                                      >  2:1984848/1‑149 (MQ=255)
                                                                                                            gggATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAg                                    >  1:1489251/1‑149 (MQ=255)
                                                                                                                tttCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCAcc                                <  2:3369125/149‑1 (MQ=255)
                                                                                                                       ggCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACa                         >  2:2776129/1‑149 (MQ=255)
                                                                                                                       ggCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACa                         >  2:2217617/1‑149 (MQ=255)
                                                                                                                       ggCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACa                         <  2:4104850/149‑1 (MQ=255)
                                                                                                                              aCATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAttt                  >  2:1440897/1‑149 (MQ=255)
                                                                                                                              aCATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAttt                  >  2:449210/1‑149 (MQ=255)
                                                                                                                               cATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAtttt                 >  1:3548851/1‑149 (MQ=255)
                                                                                                                                 tCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTgg               <  2:613070/149‑1 (MQ=255)
                                                                                                                                 tCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTgg               <  2:3425318/149‑1 (MQ=255)
                                                                                                                                      aaTGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGc          >  2:367960/1‑149 (MQ=255)
                                                                                                                                      aaTGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGc          >  2:2429622/1‑149 (MQ=255)
                                                                                                                                      aaTGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGc          >  2:4674793/1‑149 (MQ=255)
                                                                                                                                      aaTGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGc          >  2:117020/1‑149 (MQ=255)
                                                                                                                                        tGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGt        <  1:3349433/149‑1 (MQ=255)
                                                                                                                                         gATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTc       <  1:453474/149‑1 (MQ=255)
                                                                                                                                          aTCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCt      >  1:1299975/1‑149 (MQ=255)
                                                                                                                                          aTCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCt      >  1:1002981/1‑149 (MQ=255)
                                                                                                                                            cccGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtg    >  1:3064881/1‑149 (MQ=255)
                                                                                                                                             ccgccgCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtgg   <  1:1073605/149‑1 (MQ=255)
                                                                                                                                              cgcctcCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtggc  >  1:2189619/1‑149 (MQ=255)
                                                                                                                                              cgcctcCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtggc  <  2:2250559/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGC  >  CP009273/173579‑173869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑