Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 176,783 G→T 100% D354Y (GAT→TAT)  dgt → deoxyguanosine triphosphate triphosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273176,7830GT99.7% 990.3 / ‑3.5 286coding (1060/1518 nt)dgtdeoxyguanosine triphosphate triphosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (136/149);  minor base . (1/0);  total (137/149)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 294 total aligned reads displayed.

GTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCA  >  CP009273/176637‑176928
                                                                                                                                                  |                                                                                                                                                 
gTTCGCTCTTTTCGCTGGTGGTTGAAAATGTCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATttatttat                                                                                                                                                 <  2:3055414/149‑1 (MQ=255)
  tCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAACCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATaa                                                                                                                                               >  2:4201337/1‑149 (MQ=255)
     ctctTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATct                                                                                                                                            >  1:278747/1‑149 (MQ=255)
     ctctTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATct                                                                                                                                            >  2:678727/1‑149 (MQ=255)
        ttttCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATctgcc                                                                                                                                         >  2:1496183/1‑149 (MQ=255)
        ttttCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATctgcc                                                                                                                                         <  1:4592233/149‑1 (MQ=255)
         tttCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATctgcct                                                                                                                                        <  1:1075660/149‑1 (MQ=255)
         tttCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATctgcct                                                                                                                                        >  1:2431489/1‑149 (MQ=255)
                ggtggtTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGAttt                                                                                                                                 >  1:2566986/1‑149 (MQ=255)
                ggtggtTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACACTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCTAttt                                                                                                                                 <  2:2786308/149‑1 (MQ=255)
                 ttgttttaaaaTGCCTTGTAAAAATCACTCTCTTATAGTTTAAGCCGCAGTACGGTAGATCAGTTTTTTATGTATTTACGGGTAAAAACCCTAAATAAACTGGTACCCTACGCTTCACAACTATTTATTTATAATCTTCCTGCGAtttt                                                                                                                                <  1:3747115/142‑1 (MQ=255)
                 gtggtTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCTAtttt                                                                                                                                <  1:439681/149‑1 (MQ=255)
                 gtggtTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGAtttt                                                                                                                                <  2:4518225/149‑1 (MQ=255)
                 gtggtTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGAtttt                                                                                                                                <  1:2419465/149‑1 (MQ=255)
                  tggtTGAAAATGTCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTc                                                                                                                               <  1:1224728/149‑1 (MQ=255)
                    gtTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGc                                                                                                                             <  1:4040492/149‑1 (MQ=255)
                     ttGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGcc                                                                                                                            <  1:285120/149‑1 (MQ=255)
                        aaaaTGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGa                                                                                                                         >  1:3716326/1‑149 (MQ=255)
                         aaaTGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGaa                                                                                                                        <  2:1288856/149‑1 (MQ=255)
                            tGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCTATTTTCGCCGGAACGt                                                                                                                     <  1:2053762/149‑1 (MQ=255)
                               cTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTa                                                                                                                  >  1:1523600/1‑149 (MQ=255)
                                tGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTATGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTaa                                                                                                                 <  2:272575/149‑1 (MQ=255)
                                tGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTaa                                                                                                                 <  1:1857315/149‑1 (MQ=255)
                                    aaaaaTCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCAt                                                                                                             <  2:2749160/149‑1 (MQ=255)
                                     aaaaTCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATg                                                                                                            >  2:3992770/1‑149 (MQ=255)
                                         tCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCatt                                                                                                        >  2:2391909/1‑149 (MQ=255)
                                           aCGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCattat                                                                                                      <  1:324020/149‑1 (MQ=255)
                                              cTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCTATTTTCGCCGGAACGTTTAATCATGCATTATTgg                                                                                                   >  2:2024796/1‑149 (MQ=255)
                                              cTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTgg                                                                                                   >  1:3163280/1‑149 (MQ=255)
                                              cTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTgg                                                                                                   >  2:933350/1‑149 (MQ=255)
                                               tCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGa                                                                                                  >  2:3376457/1‑149 (MQ=255)
                                               tCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGa                                                                                                  <  1:2204676/149‑1 (MQ=255)
                                                cAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGaa                                                                                                 <  1:3306543/149‑1 (MQ=255)
                                                cAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGaa                                                                                                 <  2:3171620/149‑1 (MQ=255)
                                                 aaaTAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAg                                                                                                >  1:2133174/1‑149 (MQ=255)
                                                  aaTAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCTATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGa                                                                                               <  1:3513210/149‑1 (MQ=255)
                                                  aaTAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGa                                                                                               <  1:4764686/149‑1 (MQ=255)
                                                   aTAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGAt                                                                                              >  2:1195643/1‑149 (MQ=255)
                                                      gTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCAGCGATTTTCGCCGGAACGTTTAATCAAGCATTATTGGAAGATGcc                                                                                           >  2:1751915/1‑149 (MQ=255)
                                                        ttAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAg                                                                                         <  1:539775/149‑1 (MQ=255)
                                                           aGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAATTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGa                                                                                      >  2:3219722/1‑149 (MQ=255)
                                                            gCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGaa                                                                                     <  1:3423926/149‑1 (MQ=255)
                                                            gCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGaa                                                                                     >  2:3589145/1‑149 (MQ=255)
                                                                  gTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGc                                                                               >  1:1931925/1‑149 (MQ=255)
                                                                   tACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCg                                                                              <  2:3027607/149‑1 (MQ=255)
                                                                     cGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAt                                                                            <  2:3665729/149‑1 (MQ=255)
                                                                     cGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAt                                                                            >  1:226916/1‑149 (MQ=255)
                                                                       gAATATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtct                                                                          <  1:768785/149‑1 (MQ=255)
                                                                       gAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtct                                                                          <  2:2124960/149‑1 (MQ=255)
                                                                        aaGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtctt                                                                         >  2:2526279/1‑149 (MQ=255)
                                                                         aGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtcttc                                                                        >  2:3907551/1‑149 (MQ=255)
                                                                          gATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtcttct                                                                       <  1:1715620/149‑1 (MQ=255)
                                                                            tCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATcttcttg                                                                     >  1:3638028/1‑148 (MQ=255)
                                                                               gTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGc                                                                  >  2:3854100/1‑149 (MQ=255)
                                                                               gTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGc                                                                  <  2:3457092/149‑1 (MQ=255)
                                                                                ttttttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCt                                                                 >  2:4516991/1‑149 (MQ=255)
                                                                                ttttttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCAGGAACGTTTAATCATGCAGGATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCt                                                                 >  2:2885107/1‑149 (MQ=255)
                                                                                   tttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCtata                                                              <  2:1017979/149‑1 (MQ=255)
                                                                                      aggTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCTATTTTCGCCGGAACGTTTAATCATGCATGATTGGAAGATGCCAGCGACTGCAGCGATCTTCTTAAGCtatatac                                                           >  1:4395476/3‑148 (MQ=255)
                                                                                         tATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATaaaaa                                                        >  1:3795113/1‑149 (MQ=255)
                                                                                         tATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATaaaaa                                                        >  2:704231/1‑149 (MQ=255)
                                                                                          aTTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAAt                                                       <  2:4631301/149‑1 (MQ=255)
                                                                                           tttACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGACTGCAGCGATCTTCTTCAGCTATATAAAAATg                                                      >  2:1237003/1‑149 (MQ=255)
                                                                                           tttACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATg                                                      <  2:1855033/149‑1 (MQ=255)
                                                                                             tACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTc                                                    <  1:4272679/149‑1 (MQ=255)
                                                                                               cGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGc                                                  <  2:4780348/149‑1 (MQ=255)
                                                                                                    aaaCACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTaa                                             >  1:2975815/1‑149 (MQ=255)
                                                                                                    aaaCACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTaa                                             >  2:1783859/1‑149 (MQ=255)
                                                                                                     aaCACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCTATTTTCGCCGGAACGTTTAATCATGCCTTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTaaa                                            >  2:2753077/1‑149 (MQ=255)
                                                                                                        acCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCTATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACa                                         <  1:1372397/149‑1 (MQ=255)
                                                                                                        acCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACa                                         >  1:4520941/1‑149 (MQ=255)
                                                                                                         cccTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAt                                        <  2:2135376/149‑1 (MQ=255)
                                                                                                            taaataaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAtgtg                                     <  2:3768793/149‑1 (MQ=255)
                                                                                                               ataaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGttt                                  >  1:3699959/1‑149 (MQ=255)
                                                                                                                taaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTa                                 <  2:795302/149‑1 (MQ=255)
                                                                                                                 aaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCTATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAg                                >  1:1653980/1‑149 (MQ=255)
                                                                                                                        tACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCa                         <  1:4022109/149‑1 (MQ=255)
                                                                                                                        tACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCa                         >  1:3490667/1‑149 (MQ=255)
                                                                                                                        tACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCa                         <  1:2819896/149‑1 (MQ=255)
                                                                                                                         aCCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAg                        >  2:663573/1‑149 (MQ=255)
                                                                                                                          cccTACGCGGCACAACGATTTATTTATAATCTGCCTGCTATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGa                       <  2:2242024/149‑1 (MQ=255)
                                                                                                                            cTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATg                     <  1:830521/149‑1 (MQ=255)
                                                                                                                            cTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATg                     >  1:745361/1‑149 (MQ=255)
                                                                                                                            cTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATg                     >  1:3136693/1‑149 (MQ=255)
                                                                                                                             tACGCGGCACAACGATTTATTTATAATCTGCCTGCTATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGt                    <  1:3988257/149‑1 (MQ=255)
                                                                                                                              aCGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTc                   <  1:275010/149‑1 (MQ=255)
                                                                                                                                  ggCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGc               >  1:704160/1‑149 (MQ=255)
                                                                                                                                  ggCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGc               >  2:1257458/1‑149 (MQ=255)
                                                                                                                                      caACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCt           <  2:1931925/149‑1 (MQ=255)
                                                                                                                                       aaCGATTTATTTATAATCTGCCTGCTATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCtt          <  1:111161/149‑1 (MQ=255)
                                                                                                                                       aaCGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCtt          <  1:1082491/149‑1 (MQ=255)
                                                                                                                                        aCGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTg         <  2:4105822/149‑1 (MQ=255)
                                                                                                                                        aCGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTg         >  2:4067595/1‑149 (MQ=255)
                                                                                                                                           atttatttATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAAt      >  1:1318289/1‑149 (MQ=255)
                                                                                                                                            tttatttaTAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAAta     >  2:4395896/1‑148 (MQ=255)
                                                                                                                                              tATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGc   >  1:874439/1‑149 (MQ=255)
                                                                                                                                               aTTTATAATCTTCCTGCGATTTTCGCCGGAAAGTTTAATCATGAATTATTGGAAGATGCCAGCGAATGCAGCGATCTTATTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCa  <  2:2566986/149‑1 (MQ=255)
                                                                                                                                               aTTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCa  >  2:4064960/1‑149 (MQ=255)
                                                                                                                                               aTTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCa  <  1:792977/149‑1 (MQ=255)
                                                                                                                                               aTTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATGATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCa  <  2:166187/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCA  >  CP009273/176637‑176928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑