Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,098,995 C→A 100% G182* (GGA→TGA)  wbbI ← d‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,098,9950CA98.7% 1029.1 / 3.2 304G182* (GGA→TGA) wbbId‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (136/164);  total (139/165)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 304 total aligned reads displayed.

GGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGAC  >  CP009273/2098849‑2099140
                                                                                                                                                  |                                                                                                                                                 
  tttCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGCTAATCATTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCa                                                                                                                                               >  1:465868/1‑149 (MQ=255)
      gACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtat                                                                                                                                           >  1:951666/1‑149 (MQ=255)
      gACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtat                                                                                                                                           <  2:2805253/149‑1 (MQ=255)
          gAATCTCCATCTCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTatatat                                                                                                                                       <  2:4588287/149‑1 (MQ=255)
           aaTCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATg                                                                                                                                      >  1:3091928/1‑149 (MQ=255)
              ctcCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGaaa                                                                                                                                   >  2:1689397/1‑149 (MQ=255)
               tcCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAg                                                                                                                                  <  1:460953/149‑1 (MQ=255)
                  aTCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAAc                                                                                                                               >  2:3741166/1‑149 (MQ=255)
                   tCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACa                                                                                                                              >  1:1040668/1‑149 (MQ=255)
                      cAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACAttt                                                                                                                           <  1:1969175/149‑1 (MQ=255)
                       aaaTGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTa                                                                                                                          <  1:1228889/149‑1 (MQ=255)
                       aaaTGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTa                                                                                                                          >  1:2886930/1‑149 (MQ=255)
                        aaTGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTAt                                                                                                                         <  1:3872905/149‑1 (MQ=255)
                         aTGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATg                                                                                                                        >  2:1996987/1‑149 (MQ=255)
                          tGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGc                                                                                                                       <  1:4318977/149‑1 (MQ=255)
                            aGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCt                                                                                                                     >  2:632929/1‑149 (MQ=255)
                            aGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCt                                                                                                                     >  2:4417012/1‑149 (MQ=255)
                             gTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTa                                                                                                                    <  2:1804017/149‑1 (MQ=255)
                             gTCCAAATAGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTATGATTATCTTTATATTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGACTa                                                                                                                    >  1:1517790/1‑149 (MQ=255)
                              tCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAg                                                                                                                   >  1:2618263/1‑149 (MQ=255)
                                  aaTTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAg                                                                                                               <  2:1010452/149‑1 (MQ=255)
                                  aaTTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAg                                                                                                               >  2:531235/1‑149 (MQ=255)
                                  aaTTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAg                                                                                                               <  1:4203523/149‑1 (MQ=255)
                                   aTTGCATGCATGGGATTTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAgg                                                                                                              <  2:2580167/149‑1 (MQ=255)
                                    ttGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGt                                                                                                             <  2:4452894/149‑1 (MQ=255)
                                    ttGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGt                                                                                                             >  2:303347/1‑149 (MQ=255)
                                      gCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTg                                                                                                           >  2:1550495/1‑149 (MQ=255)
                                      gCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAG‑AAGGTTGc                                                                                                          >  2:1057273/1‑149 (MQ=255)
                                            cTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCa                                                                                                     >  1:1041528/1‑149 (MQ=255)
                                             tGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCat                                                                                                    <  2:4415257/149‑1 (MQ=255)
                                             tGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCat                                                                                                    >  2:2645104/1‑149 (MQ=255)
                                              gggAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCata                                                                                                   <  1:3419923/149‑1 (MQ=255)
                                              gggAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCata                                                                                                   >  1:1592154/1‑149 (MQ=255)
                                                gAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCatata                                                                                                 >  1:587071/1‑149 (MQ=255)
                                                  ggTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATg                                                                                               >  1:303065/1‑149 (MQ=255)
                                                   gTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGa                                                                                              <  2:2241909/149‑1 (MQ=255)
                                                   gTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGa                                                                                              <  2:1746306/149‑1 (MQ=255)
                                                      aaTCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAccc                                                                                           <  2:140491/149‑1 (MQ=255)
                                                        tCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATTACCCCt                                                                                         <  2:3034713/149‑1 (MQ=255)
                                                         cTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTc                                                                                        <  1:3981776/149‑1 (MQ=255)
                                                           tttCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGt                                                                                      <  2:3991789/149‑1 (MQ=255)
                                                                 gagaTTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTa                                                                                <  1:3628760/149‑1 (MQ=255)
                                                                   gaTTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATc                                                                              <  1:3232506/149‑1 (MQ=255)
                                                                   gaTTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATc                                                                              <  2:1015815/149‑1 (MQ=255)
                                                                       gAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTa                                                                          >  1:2783827/1‑149 (MQ=255)
                                                                          cATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACa                                                                       >  1:745671/1‑149 (MQ=255)
                                                                           aTCAATACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCACAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAt                                                                      <  2:2746773/149‑1 (MQ=255)
                                                                             cAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtct                                                                    <  2:4490061/149‑1 (MQ=255)
                                                                              aaaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtctc                                                                   >  1:4001305/1‑149 (MQ=255)
                                                                              aaaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtctc                                                                   <  1:21300/149‑1 (MQ=255)
                                                                               aaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCg                                                                  >  1:249884/1‑149 (MQ=255)
                                                                                aaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGa                                                                 >  2:2671182/1‑149 (MQ=255)
                                                                                   ttCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGc                                                              >  2:1487966/1‑149 (MQ=255)
                                                                                     ccAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTc                                                            >  2:4246358/1‑149 (MQ=255)
                                                                                      cAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCATTATATATGAAAGTACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTcc                                                           <  2:3348901/149‑1 (MQ=255)
                                                                                           tatTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATc                                                      <  2:3647156/149‑1 (MQ=255)
                                                                                           tatTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATc                                                      >  1:1782436/1‑149 (MQ=255)
                                                                                             tttAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAg                                                    >  2:4045541/1‑149 (MQ=255)
                                                                                             tttAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAg                                                    >  2:1964110/1‑149 (MQ=255)
                                                                                                   aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgaga                                              <  2:2886930/149‑1 (MQ=255)
                                                                                                   aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgaga                                              <  2:3100180/149‑1 (MQ=255)
                                                                                                   aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgaga                                              <  2:494921/149‑1 (MQ=255)
                                                                                                   aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgaga                                              <  2:55519/149‑1 (MQ=255)
                                                                                                   aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgaga                                              <  2:872402/149‑1 (MQ=255)
                                                                                                   aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgaga                                              <  1:1124110/149‑1 (MQ=255)
                                                                                                   aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgaga                                              <  1:674698/149‑1 (MQ=255)
                                                                                                    ttATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGAc                                             >  2:2050083/1‑149 (MQ=255)
                                                                                                    ttATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGAc                                             >  2:4551578/1‑149 (MQ=255)
                                                                                                    ttATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGAc                                             >  1:3527013/1‑149 (MQ=255)
                                                                                                      aTCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGa                                           >  2:1509116/1‑149 (MQ=255)
                                                                                                         tttATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGt                                        >  1:2978807/1‑149 (MQ=255)
                                                                                                           tATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTaa                                      <  2:473167/149‑1 (MQ=255)
                                                                                                           tATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCAGTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTaa                                      <  2:1833301/149‑1 (MQ=255)
                                                                                                             ttttCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATc                                    <  1:4258992/149‑1 (MQ=255)
                                                                                                             ttttCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATc                                    <  1:4127325/149‑1 (MQ=255)
                                                                                                                tCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCaaa                                 >  2:436035/1‑149 (MQ=255)
                                                                                                                  aTAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAata                               <  1:4505005/149‑1 (MQ=255)
                                                                                                                  aTAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAata                               <  1:2670997/149‑1 (MQ=255)
                                                                                                                  aTAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAata                               <  2:3197212/149‑1 (MQ=255)
                                                                                                                   tAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAatat                              >  1:3948166/1‑149 (MQ=255)
                                                                                                                   tAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAatat                              >  1:1558761/1‑149 (MQ=255)
                                                                                                                       tGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATAttttt                          <  1:169716/149‑1 (MQ=255)
                                                                                                                        gACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTa                         >  1:4106166/1‑149 (MQ=255)
                                                                                                                         acacCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTAt                        >  1:1402058/1‑149 (MQ=255)
                                                                                                                         acacCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTAt                        <  1:2019669/149‑1 (MQ=255)
                                                                                                                         acacCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTAt                        <  2:1234239/149‑1 (MQ=255)
                                                                                                                            ccAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTc                     <  1:2620585/149‑1 (MQ=255)
                                                                                                                                agagagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTg                 >  1:3766723/1‑149 (MQ=255)
                                                                                                                                  agagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAt               <  2:4166197/149‑1 (MQ=255)
                                                                                                                                   gagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtt              <  1:3124290/149‑1 (MQ=255)
                                                                                                                                   gagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtt              >  2:422438/1‑149 (MQ=255)
                                                                                                                                    agTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAttt             <  2:2457804/149‑1 (MQ=255)
                                                                                                                                      tAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTa           <  2:1603223/149‑1 (MQ=255)
                                                                                                                                        aaaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATc         >  1:3566040/1‑149 (MQ=255)
                                                                                                                                        aaaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATc         <  1:2192154/149‑1 (MQ=255)
                                                                                                                                         aaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATcc        >  2:3540636/1‑149 (MQ=255)
                                                                                                                                          aTCGCATCATTCAGTATATATTAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCt       <  2:4205610/149‑1 (MQ=255)
                                                                                                                                          aTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCt       <  2:3873404/149‑1 (MQ=255)
                                                                                                                                          aTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCt       <  1:608757/149‑1 (MQ=255)
                                                                                                                                          aTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCt       <  2:2550739/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGAC  >  CP009273/2098849‑2099140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑