Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,110,346 G→A 100% T88I (ACC→ATC)  wcaL ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,110,3460GA99.2% 887.8 / ‑2.4 254T88I (ACC→ATC) wcaLputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (127/125);  total (128/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 255 total aligned reads displayed.

GGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCC  >  CP009273/2110200‑2110491
                                                                                                                                                  |                                                                                                                                                 
ggAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATa                                                                                                                                                 >  2:822316/1‑149 (MQ=255)
ggAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATa                                                                                                                                                 >  1:322573/1‑149 (MQ=255)
  aGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAtt                                                                                                                                               <  2:4464858/149‑1 (MQ=255)
     tttGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAttttt                                                                                                                                            >  2:4768999/1‑149 (MQ=255)
      ttGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTa                                                                                                                                           <  1:4503751/149‑1 (MQ=255)
       tgctgcGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTTCGACCTGGCCGCAATTGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTAc                                                                                                                                          <  2:1352994/149‑1 (MQ=255)
       tgctgcGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTAc                                                                                                                                          <  1:3998094/149‑1 (MQ=255)
       tgctgcGGTTACCCCCGCGGGACAAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAATTGGCAGAAAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTAc                                                                                                                                          <  1:2006636/149‑1 (MQ=255)
          tgcGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGAt                                                                                                                                       >  2:2095877/1‑149 (MQ=255)
               ttACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCTATTGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACTATGAATg                                                                                                                                  <  1:1456243/149‑1 (MQ=255)
               ttACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATg                                                                                                                                  <  1:3525240/149‑1 (MQ=255)
                tACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGc                                                                                                                                 >  2:47089/1‑149 (MQ=255)
                 aCCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTATTTTTACGATGAATGcc                                                                                                                                >  2:570939/1‑149 (MQ=255)
                  cccccGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCcg                                                                                                                               >  2:3141244/1‑149 (MQ=255)
                  cccccGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCcg                                                                                                                               >  2:2798634/1‑149 (MQ=255)
                   ccccGCGGGACCAAAATGAGCGATGAACACCTCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCcgc                                                                                                                              <  2:1247146/149‑1 (MQ=255)
                    cccGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATCTTTTTACGATGAATGCcgcg                                                                                                                             >  1:2926800/1‑149 (MQ=255)
                     ccGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCgcgc                                                                                                                            >  1:1995131/1‑149 (MQ=255)
                       gcgGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCaa                                                                                                                          <  2:259968/149‑1 (MQ=255)
                       gcgGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCaa                                                                                                                          <  1:1436238/149‑1 (MQ=255)
                             ccAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctg                                                                                                                    >  1:2318888/1‑149 (MQ=255)
                              cAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctgg                                                                                                                   <  2:3181715/149‑1 (MQ=255)
                               aaaaTGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctggc                                                                                                                  <  2:276034/149‑1 (MQ=255)
                               aaaaTGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctggc                                                                                                                  <  1:2780474/149‑1 (MQ=255)
                                   tGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCtggctggc                                                                                                              >  2:623947/1‑149 (MQ=255)
                                    gAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTggctggct                                                                                                             <  2:158178/149‑1 (MQ=255)
                                    gAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTggctggct                                                                                                             <  2:2820960/149‑1 (MQ=255)
                                     aGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTc                                                                                                            <  2:1727904/149‑1 (MQ=255)
                                      gCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCg                                                                                                           >  1:1615033/1‑149 (MQ=255)
                                        gATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGt                                                                                                         <  2:2191952/149‑1 (MQ=255)
                                           gAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGTCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGgc                                                                                                      >  2:2986004/1‑149 (MQ=255)
                                            aaCACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGgcg                                                                                                     >  1:682467/1‑149 (MQ=255)
                                             acacATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGgcgc                                                                                                    >  1:2654515/1‑149 (MQ=255)
                                              cacaTCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCa                                                                                                   >  2:2353091/1‑149 (MQ=255)
                                              cacaTCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCa                                                                                                   >  1:1915780/1‑149 (MQ=255)
                                                  tCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGttt                                                                                               <  2:412835/149‑1 (MQ=255)
                                                     gcgcGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGc                                                                                            >  2:273289/1‑149 (MQ=255)
                                                          aaaCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCTGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACtt                                                                                       <  1:538769/149‑1 (MQ=255)
                                                          aaaCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACtt                                                                                       >  2:1041161/1‑149 (MQ=255)
                                                          aaaCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACtt                                                                                       >  1:1315515/1‑149 (MQ=255)
                                                          aaaCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACtt                                                                                       >  2:3856107/1‑149 (MQ=255)
                                                            aCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTg                                                                                     >  1:4101627/1‑149 (MQ=255)
                                                             cGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTTGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGc                                                                                    <  1:436178/149‑1 (MQ=255)
                                                               gtgtTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGccc                                                                                  >  1:2931066/1‑149 (MQ=255)
                                                                 gtTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGt                                                                                >  1:954891/1‑149 (MQ=255)
                                                                  ttGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGtg                                                                               <  2:3655664/149‑2 (MQ=255)
                                                                  ttGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTa                                                                               >  2:1958490/1‑149 (MQ=255)
                                                                  ttGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCAGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTa                                                                               <  1:4429269/149‑1 (MQ=255)
                                                                     cGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCCAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGt                                                                            <  2:2679644/149‑1 (MQ=255)
                                                                      gACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGtt                                                                           <  2:3038840/149‑1 (MQ=255)
                                                                      gACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGtt                                                                           >  1:3408182/1‑149 (MQ=255)
                                                                      gACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGtt                                                                           <  1:4477838/149‑1 (MQ=255)
                                                                       aCCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTc                                                                          >  1:4731864/1‑149 (MQ=255)
                                                                        ccTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCg                                                                         >  2:375798/1‑149 (MQ=255)
                                                                         cTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGt                                                                        <  2:1641279/149‑1 (MQ=255)
                                                                          tGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTc                                                                       <  1:1759950/149‑1 (MQ=255)
                                                                           ggCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTcc                                                                      >  1:1870670/1‑149 (MQ=255)
                                                                             ccGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTg                                                                    >  1:2435360/1‑149 (MQ=255)
                                                                               gCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTa                                                                  >  2:4236295/1‑149 (MQ=255)
                                                                                  aaTGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAcc                                                               >  1:330097/1‑149 (MQ=255)
                                                                                   aTGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCa                                                              >  1:1133611/1‑149 (MQ=255)
                                                                                     ggCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGc                                                            >  2:2547681/1‑149 (MQ=255)
                                                                                       cAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCgg                                                          <  1:4256021/149‑1 (MQ=255)
                                                                                        aGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCggg                                                         <  2:320322/149‑1 (MQ=255)
                                                                                         gACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGt                                                        <  1:422325/149‑1 (MQ=255)
                                                                                          aCAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGtt                                                       <  1:800334/149‑1 (MQ=255)
                                                                                          aCAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGtt                                                       >  2:801402/1‑149 (MQ=255)
                                                                                           cAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTc                                                      <  1:628636/149‑1 (MQ=255)
                                                                                            aaaaTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCt                                                     <  2:719465/149‑1 (MQ=255)
                                                                                             aaaTCAGGGTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTg                                                    <  1:1735578/149‑1 (MQ=255)
                                                                                              aaTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTgg                                                   >  1:1883859/1‑149 (MQ=255)
                                                                                                    gTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGTCGCAGTTTCGCAACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCaa                                             <  1:570939/149‑1 (MQ=255)
                                                                                                    gTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCaa                                             >  1:228204/1‑149 (MQ=255)
                                                                                                         gcgACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTg                                        <  1:4717367/149‑1 (MQ=255)
                                                                                                            aCTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAt                                     >  2:4604648/1‑149 (MQ=255)
                                                                                                             cTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAtt                                    <  1:4641240/149‑1 (MQ=255)
                                                                                                               cGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTc                                  <  1:118631/149‑1 (MQ=255)
                                                                                                               cGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTc                                  <  1:3866243/149‑1 (MQ=255)
                                                                                                                 gCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGt                                >  1:2868145/1‑149 (MQ=255)
                                                                                                                    ccATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCa                             >  1:503144/1‑149 (MQ=255)
                                                                                                                    ccATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCa                             >  2:428296/1‑149 (MQ=255)
                                                                                                                    ccATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCa                             >  2:4360694/1‑149 (MQ=255)
                                                                                                                     cATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCAt                            <  1:2074819/149‑1 (MQ=255)
                                                                                                                         gCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCcg                        <  1:301845/149‑1 (MQ=255)
                                                                                                                          cGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCcgc                       <  1:1284507/149‑1 (MQ=255)
                                                                                                                           gTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCcgcg                      >  2:3557353/1‑149 (MQ=255)
                                                                                                                             ttGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCCAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGGAGGTTCGTCCTGTAACCAGCGGGGTCTGGCAGCAAGGGTGTATTTCGTCCATGCCGCGTg                    >  2:441520/1‑149 (MQ=255)
                                                                                                                              tGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTgg                   <  1:211885/149‑1 (MQ=255)
                                                                                                                                 ggTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGgtg                >  2:4640673/1‑149 (MQ=255)
                                                                                                                                 ggTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGgtg                >  2:2362112/1‑149 (MQ=255)
                                                                                                                                  gTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGgtgt               <  2:3131500/149‑1 (MQ=255)
                                                                                                                                  gTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGgtgt               <  2:1807902/149‑1 (MQ=255)
                                                                                                                                   ttGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGtt              >  1:2666700/1‑149 (MQ=255)
                                                                                                                                    tGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGttt             <  2:672183/149‑1 (MQ=255)
                                                                                                                                    tGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGttt             <  1:3112282/149‑1 (MQ=255)
                                                                                                                                      aGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtg           <  2:322573/149‑1 (MQ=255)
                                                                                                                                        cgcCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgtg         >  1:1547907/1‑149 (MQ=255)
                                                                                                                                           cTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTgtgtgt      <  1:822372/149‑1 (MQ=255)
                                                                                                                                           cTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTgtgtgt      <  2:1095472/149‑1 (MQ=255)
                                                                                                                                            tGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTc     >  1:734935/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCC  >  CP009273/2110200‑2110491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑