BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,144,474	C→A	100%	intergenic (‑8/‑192)	yegI ← / → yegJ	protein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,144,474	0	C	A	99.2%	860.5 / ‑1.7	250	intergenic (‑8/‑192)	yegI/yegJ	protein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (115/133); total (116/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 250 total aligned reads displayed.

GTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTCCA  >  CP009273/2144333‑2144621

gtgtGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTa                                                                                                                                              <  2:1146796/149‑1 (MQ=255)

gtgtGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTa                                                                                                                                              <  1:3861442/149‑1 (MQ=255)

 tgtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAg                                                                                                                                             >  2:1488058/1‑149 (MQ=255)

   tgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGtt                                                                                                                                           >  2:3191886/1‑149 (MQ=255)

   tgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGtt                                                                                                                                           <  1:3128040/149‑1 (MQ=255)

   tgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGtt                                                                                                                                           >  1:4321939/1‑149 (MQ=255)

    gATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGtta                                                                                                                                          <  2:4370837/149‑1 (MQ=255)

      tAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttata                                                                                                                                        <  2:2264573/149‑1 (MQ=255)

       aaaTCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatat                                                                                                                                       <  2:501242/149‑1 (MQ=255)

       aaaTCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatat                                                                                                                                       >  1:682974/1‑149 (MQ=255)

          tCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatattat                                                                                                                                    <  1:3964430/149‑1 (MQ=255)

            ttGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTtatattatat                                                                                                                                  <  2:4585650/149‑1 (MQ=255)

             tGGCGACGCTATCGACAAACTCCTCGAGATCATAAACCGCGCCTTCACCACCTGGGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTatattatatt                                                                                                                                 <  2:3629841/149‑1 (MQ=255)

              ggCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATAttt                                                                                                                                <  1:555309/149‑1 (MQ=255)

                 gACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCaa                                                                                                                             >  2:1575261/1‑149 (MQ=255)

                 gACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCaa                                                                                                                             <  2:313469/149‑1 (MQ=255)

                  aCGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAAt                                                                                                                            >  1:1772918/1‑149 (MQ=255)

                     cTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaa                                                                                                                         <  1:4743002/149‑1 (MQ=255)

                     cTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaa                                                                                                                         <  2:2033131/149‑1 (MQ=255)

                       aTCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaata                                                                                                                       >  2:896593/1‑149 (MQ=255)

                        tCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaataa                                                                                                                      <  1:1675743/149‑1 (MQ=255)

                         cGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaataat                                                                                                                     <  1:3434797/149‑1 (MQ=255)

                          gACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAataata                                                                                                                    <  1:4216901/149‑1 (MQ=255)

                          gACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAataata                                                                                                                    >  2:4448980/1‑149 (MQ=255)

                          gACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAataata                                                                                                                    >  2:407486/1‑149 (MQ=255)

                           aCAAACTCCTCGATATCATAAATCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAAGTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAAtaataa                                                                                                                   <  2:4165481/149‑1 (MQ=255)

                           aCAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAAtaataa                                                                                                                   <  1:3161538/149‑1 (MQ=255)

                           aCAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAAtaataa                                                                                                                   >  2:2819232/1‑149 (MQ=255)

                            cAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATaac                                                                                                                  <  1:1542934/149‑1 (MQ=255)

                            cAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATaac                                                                                                                  <  1:2415284/149‑1 (MQ=255)

                              aaCTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATaacaa                                                                                                                >  1:1745783/1‑149 (MQ=255)

                                ctcctcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACt                                                                                                              <  1:2486165/149‑1 (MQ=255)

                                ctcctcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACt                                                                                                              >  2:3508557/1‑149 (MQ=255)

                                   ctcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAAc                                                                                                           <  2:2055030/149‑1 (MQ=255)

                                    tcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAAAACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAAcc                                                                                                          >  1:1497032/1‑149 (MQ=255)

                                      gATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATGTTATATTTCAATGAATAATAACAACTAACCAt                                                                                                        <  1:1937356/149‑1 (MQ=255)

                                       atatCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAtt                                                                                                       <  1:4078262/149‑1 (MQ=255)

                                       atatCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAtt                                                                                                       <  2:561356/149‑1 (MQ=255)

                                        tatCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTa                                                                                                      <  1:1430238/149‑1 (MQ=255)

                                                aCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAattatt                                                                                              <  2:3876518/149‑1 (MQ=255)

                                                 ccGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTAttt                                                                                             >  2:2958806/1‑149 (MQ=255)

                                                  cgcgCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTc                                                                                            <  1:48691/149‑1 (MQ=255)

                                                    cgcCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCta                                                                                          <  2:2472795/149‑1 (MQ=255)

                                                      ccTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCtata                                                                                        <  1:1177670/149‑1 (MQ=255)

                                                      ccTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGAGGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCtata                                                                                        >  1:8685/1‑149 (MQ=255)

                                                        tgcaccacCATTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCGGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATg                                                                                      <  2:2529609/147‑1 (MQ=255)

                                                          caccacCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATggg                                                                                    <  2:3838552/149‑1 (MQ=255)

                                                          caccacCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATggg                                                                                    >  2:3247540/1‑149 (MQ=255)

                                                           accaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATgggg                                                                                   <  2:3468526/149‑1 (MQ=255)

                                                            ccaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGa                                                                                  >  2:1084928/1‑149 (MQ=255)

                                                                cTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAAAAAATAACCATTAAAATTATTTATATATGGGGAAAAc                                                                              >  1:1611242/1‑149 (MQ=255)

                                                                  ttGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAAccc                                                                            <  1:1823736/149‑1 (MQ=255)

                                                                    gCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAAACATTAAAATTATTTCTATATGGGGAAAACCCAc                                                                          >  1:2818935/1‑149 (MQ=255)

                                                                        aGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCaa                                                                      <  2:2713559/149‑1 (MQ=255)

                                                                        aGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCaa                                                                      >  2:4348156/1‑149 (MQ=255)

                                                                           tCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTATCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAATCCCACTCAAGaa                                                                   >  2:770439/1‑149 (MQ=255)

                                                                              cGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaa                                                                >  1:2599494/1‑149 (MQ=255)

                                                                              cGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaa                                                                <  1:896572/149‑1 (MQ=255)

                                                                              cGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaa                                                                >  1:331889/1‑149 (MQ=255)

                                                                               ggCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaaa                                                               <  1:3993532/149‑1 (MQ=255)

                                                                                gCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaaaa                                                              >  1:1202626/1‑149 (MQ=255)

                                                                                  cAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAAt                                                            >  1:3481051/1‑149 (MQ=255)

                                                                                   aaGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATg                                                           <  1:4163411/149‑1 (MQ=255)

                                                                                       gTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATaa                                                       >  1:3058012/1‑149 (MQ=255)

                                                                                         ggTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATg                                                     >  1:3023975/1‑149 (MQ=255)

                                                                                          gTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGc                                                    >  2:176906/1‑149 (MQ=255)

                                                                                          gTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGc                                                    <  1:3525963/149‑1 (MQ=255)

                                                                                            cAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCaa                                                  <  2:320564/149‑1 (MQ=255)

                                                                                              aTTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAtt                                                >  2:685711/1‑149 (MQ=255)

                                                                                              aTTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAtt                                                <  2:3022221/149‑1 (MQ=255)

                                                                                                  aCCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTAt                                            <  2:3610739/149‑1 (MQ=255)

                                                                                                   ccTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATc                                           <  2:3634047/149‑1 (MQ=255)

                                                                                                    cTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCa                                          >  2:1811641/1‑149 (MQ=255)

                                                                                                    cTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCa                                          >  1:2198897/1‑149 (MQ=255)

                                                                                                      gTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAgg                                        >  1:2041967/1‑149 (MQ=255)

                                                                                                          aTGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAc                                    >  1:533674/1‑149 (MQ=255)

                                                                                                             tAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaat                                 >  2:928529/1‑149 (MQ=255)

                                                                                                              aaaTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaatt                                <  2:466071/149‑1 (MQ=255)

                                                                                                              aaaTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaatt                                <  2:2755564/149‑1 (MQ=255)

                                                                                                               aaTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattc                               >  1:1084872/1‑149 (MQ=255)

                                                                                                                aTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattca                              >  1:3239048/1‑149 (MQ=255)

                                                                                                                aTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattca                              >  1:3356137/1‑149 (MQ=255)

                                                                                                                 tACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattcaa                             >  1:2341121/1‑149 (MQ=255)

                                                                                                                  aCTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattcaat                            >  1:1058561/1‑149 (MQ=255)

                                                                                                                   cTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattcaatt                           >  1:3286879/1‑149 (MQ=255)

                                                                                                                      ttATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCg                        <  1:3372201/149‑1 (MQ=255)

                                                                                                                        atatTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGc                      >  2:819591/1‑149 (MQ=255)

                                                                                                                         tatTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGcc                     >  1:3333933/1‑149 (MQ=255)

                                                                                                                         tatTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGcc                     >  1:3574795/1‑149 (MQ=255)

                                                                                                                            ttGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGa                  <  2:1220257/149‑1 (MQ=255)

                                                                                                                             tGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGAt                 >  2:2281923/1‑149 (MQ=255)

                                                                                                                               ttttCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATaa               >  1:37298/1‑149 (MQ=255)

                                                                                                                               ttttCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATaa               <  1:899524/149‑1 (MQ=255)

                                                                                                                               ttttCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATaa               <  1:1582045/149‑1 (MQ=255)

                                                                                                                                  tCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGa            >  1:3103817/1‑149 (MQ=255)

                                                                                                                                       atatTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAAc       >  1:4679328/1‑149 (MQ=255)

                                                                                                                                        tatTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACt      >  1:2258821/1‑149 (MQ=255)

                                                                                                                                        tatTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACt      >  1:93237/1‑149 (MQ=255)

                                                                                                                                         atTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACtt     >  1:60836/1‑149 (MQ=255)

                                                                                                                                          tttAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTc    >  1:1373863/1‑149 (MQ=255)

GTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTCCA  >  CP009273/2144333‑2144621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`