Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,149,603 A→T 100% E287V (GAA→GTA)  mdtB → multidrug efflux system, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,149,6030AT99.3% 956.9 / ‑2.7 279E287V (GAA→GTA) mdtBmultidrug efflux system, subunit B
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (148/129);  total (150/129)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 279 total aligned reads displayed.

CCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAAT  >  CP009273/2149459‑2149748
                                                                                                                                                |                                                                                                                                                 
ccGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAg                                                                                                                                               >  2:3135445/1‑149 (MQ=255)
    gAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCa                                                                                                                                           >  1:3679213/1‑149 (MQ=255)
    gAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCa                                                                                                                                           >  1:2225773/1‑149 (MQ=255)
    gAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCa                                                                                                                                           >  2:1450837/1‑149 (MQ=255)
      aCGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAtt                                                                                                                                         <  2:312073/149‑1 (MQ=255)
      aCGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAtt                                                                                                                                         >  1:3438627/1‑149 (MQ=255)
        gACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATtgt                                                                                                                                       >  2:2900351/1‑149 (MQ=255)
          ccAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtga                                                                                                                                     >  1:4105379/1‑149 (MQ=255)
          ccAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtga                                                                                                                                     <  2:2547480/149‑1 (MQ=255)
            aGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtgatg                                                                                                                                   <  2:2468567/149‑1 (MQ=255)
            aGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtgatg                                                                                                                                   <  1:1156672/149‑1 (MQ=255)
            aGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtgatg                                                                                                                                   <  1:3192498/149‑1 (MQ=255)
            aGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGAAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtgatg                                                                                                                                   >  1:3743293/1‑149 (MQ=255)
              aTGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGaa                                                                                                                                 <  2:4176374/149‑1 (MQ=255)
                  aaTCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGtt                                                                                                                             >  2:4484148/1‑149 (MQ=255)
                   aTCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTc                                                                                                                            >  2:158686/1‑149 (MQ=255)
                   aTCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTc                                                                                                                            >  1:4397370/1‑149 (MQ=255)
                   aTCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTc                                                                                                                            <  1:720781/149‑1 (MQ=255)
                      cgccgAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAgc                                                                                                                         <  2:4315742/149‑1 (MQ=255)
                      cgccgAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAgc                                                                                                                         <  2:1196544/149‑1 (MQ=255)
                       gccgAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAgcg                                                                                                                        >  1:1492477/1‑149 (MQ=255)
                         cgAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGcc                                                                                                                      >  1:2878799/1‑149 (MQ=255)
                           aaGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAg                                                                                                                    >  1:2683518/1‑149 (MQ=255)
                           aaGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAg                                                                                                                    >  1:1744183/1‑149 (MQ=255)
                            agagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGc                                                                                                                   >  2:297558/1‑149 (MQ=255)
                               gTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCg                                                                                                                >  1:4517121/1‑149 (MQ=255)
                                    gCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCt                                                                                                           <  1:2553255/149‑1 (MQ=255)
                                      cAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTaa                                                                                                         <  1:3225384/149‑1 (MQ=255)
                                      cAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTaa                                                                                                         <  2:2575036/149‑1 (MQ=255)
                                       aGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAAc                                                                                                        >  1:2405719/1‑149 (MQ=255)
                                        gCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACa                                                                                                       >  1:4691931/1‑149 (MQ=255)
                                            atcatcGCCTACCAGAACGGCGCGCCAAGTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAt                                                                                                   <  1:965392/149‑1 (MQ=255)
                                             tcatcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtc                                                                                                  >  1:2625486/1‑149 (MQ=255)
                                              catcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtct                                                                                                 <  2:288234/149‑1 (MQ=255)
                                               atcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtctc                                                                                                >  1:2013539/1‑149 (MQ=255)
                                               atcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtctc                                                                                                <  2:3422481/149‑1 (MQ=255)
                                                tcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTcc                                                                                               >  1:4753611/1‑149 (MQ=255)
                                                tcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTcc                                                                                               >  1:4147664/1‑149 (MQ=255)
                                                 cGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCa                                                                                              <  2:4027470/149‑1 (MQ=255)
                                                  gCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAc                                                                                             >  2:1777871/1‑149 (MQ=255)
                                                     tACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAccgc                                                                                          <  1:4654394/149‑1 (MQ=255)
                                                      aCCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAccgcc                                                                                         >  2:3257753/1‑149 (MQ=255)
                                                          gAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACa                                                                                     <  2:3050815/149‑1 (MQ=255)
                                                          gAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACa                                                                                     >  2:2103854/1‑149 (MQ=255)
                                                          gAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACa                                                                                     <  1:2294948/149‑1 (MQ=255)
                                                            aCGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGc                                                                                   >  2:1963013/1‑149 (MQ=255)
                                                            aCGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGc                                                                                   >  2:1043646/1‑149 (MQ=255)
                                                                 gcgcCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCg                                                                              <  2:2597454/149‑1 (MQ=255)
                                                                      aaTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGTGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGa                                                                         >  2:3898978/1‑149 (MQ=255)
                                                                      aaTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGa                                                                         >  1:4049839/1‑149 (MQ=255)
                                                                      aaTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGa                                                                         <  1:2181429/149‑1 (MQ=255)
                                                                         tCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgc                                                                      <  1:4022029/149‑1 (MQ=255)
                                                                            tCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgctgc                                                                   >  2:4616369/1‑149 (MQ=255)
                                                                               gggCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCcac                                                                <  1:2708212/149‑1 (MQ=255)
                                                                               gggCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCcac                                                                <  1:4348357/149‑1 (MQ=255)
                                                                                   gATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCt                                                            >  2:277302/1‑149 (MQ=255)
                                                                                   gATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCt                                                            >  1:2860268/1‑149 (MQ=255)
                                                                                      gTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCAc                                                         <  2:1738886/149‑1 (MQ=255)
                                                                                       tCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACt                                                        <  1:1608168/149‑1 (MQ=255)
                                                                                        cGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTg                                                       >  2:1155802/1‑149 (MQ=255)
                                                                                         gCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTga                                                      >  1:3827280/1‑149 (MQ=255)
                                                                                           aaCTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTgaga                                                    >  2:647052/1‑149 (MQ=255)
                                                                                           aaCTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTgaga                                                    >  2:4516407/1‑149 (MQ=255)
                                                                                            aCTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGagag                                                   <  2:1215539/149‑1 (MQ=255)
                                                                                             cTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGt                                                  >  1:66082/1‑149 (MQ=255)
                                                                                                tAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTg                                               >  1:1282551/1‑149 (MQ=255)
                                                                                                 agagCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGc                                              >  1:1192474/1‑149 (MQ=255)
                                                                                                   agCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCg                                            >  1:1013761/1‑149 (MQ=255)
                                                                                                     cAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGaa                                          >  2:2699115/1‑149 (MQ=255)
                                                                                                      aaGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGaaa                                         <  1:1431886/149‑1 (MQ=255)
                                                                                                       aGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAAt                                        <  2:2336652/149‑1 (MQ=255)
                                                                                                        ggTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATc                                       >  1:4772101/1‑149 (MQ=255)
                                                                                                          tGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCgg                                     <  2:3508404/149‑1 (MQ=255)
                                                                                                             aGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGa                                  >  1:4488928/1‑149 (MQ=255)
                                                                                                              gAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGaa                                 >  1:2652679/1‑149 (MQ=255)
                                                                                                               aaaaCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAg                                <  2:2181441/149‑1 (MQ=255)
                                                                                                                aaaCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAgg                               >  1:752307/1‑149 (MQ=255)
                                                                                                                aaaCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAgg                               <  2:1743909/149‑1 (MQ=255)
                                                                                                                aaaCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAgg                               >  2:1817384/1‑149 (MQ=255)
                                                                                                                aaaCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAgg                               <  1:1347033/149‑1 (MQ=255)
                                                                                                                 aaCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGt                              >  2:41003/1‑149 (MQ=255)
                                                                                                                  aCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTg                             >  2:2630308/1‑149 (MQ=255)
                                                                                                                  aCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTg                             >  2:1719743/1‑149 (MQ=255)
                                                                                                                   cAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGa                            <  1:2663619/149‑1 (MQ=255)
                                                                                                                    aGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGAc                           <  2:3207931/149‑1 (MQ=255)
                                                                                                                        ggCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTg                       >  1:1115118/1‑149 (MQ=255)
                                                                                                                        ggCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTg                       <  1:2900351/149‑1 (MQ=255)
                                                                                                                         gCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGc                      <  1:833047/149‑1 (MQ=255)
                                                                                                                          cTCGGCGCGTGGGCTAACACAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGTGTCTGCCGAAATCGGTGAAGGTGACAGTGCt                     <  2:3585394/149‑1 (MQ=255)
                                                                                                                              gcgcGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTcc                 >  2:3053937/1‑149 (MQ=255)
                                                                                                                              gcgcGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTcc                 <  1:3081398/149‑1 (MQ=255)
                                                                                                                                gcgTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGa               >  1:3118623/1‑149 (MQ=255)
                                                                                                                                 cgTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGAt              <  2:1330286/149‑1 (MQ=255)
                                                                                                                                 cgTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGAt              <  2:4737447/149‑1 (MQ=255)
                                                                                                                                       cGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcacc        <  2:4147664/149‑1 (MQ=255)
                                                                                                                                        gAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcacca       >  2:2438989/1‑149 (MQ=255)
                                                                                                                                        gAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcacca       >  1:4193519/1‑149 (MQ=255)
                                                                                                                                         aaCAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcaccac      >  1:2784984/1‑149 (MQ=255)
                                                                                                                                         aaCAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcaccac      <  2:769629/149‑1 (MQ=255)
                                                                                                                                            aaaGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCaa   <  2:3679213/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAAT  >  CP009273/2149459‑2149748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑