Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,181,645 A→G 100% N83N (AAT→AAC)  yehA ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,181,6450AG98.7% 1167.6 / 1.0 294N83N (AAT→AAC) yehAputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base G (130/160);  total (133/161)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 309 total aligned reads displayed.

ATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCT  >  CP009273/2181499‑2181790
                                                                                                                                                  |                                                                                                                                                 
atcatcACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtt                                                                                                                                                 >  1:1198547/1‑149 (MQ=255)
atcatcACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtt                                                                                                                                                 <  2:4665546/149‑1 (MQ=255)
atcatcACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtt                                                                                                                                                 <  2:166113/149‑1 (MQ=255)
   atcaCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaa                                                                                                                                              <  2:2315144/149‑1 (MQ=255)
    tcaCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaat                                                                                                                                             <  1:4149732/149‑1 (MQ=255)
      aCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaattt                                                                                                                                           <  2:2475686/149‑1 (MQ=255)
        tGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTa                                                                                                                                         <  2:1116787/149‑1 (MQ=255)
          aaCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATg                                                                                                                                       <  2:3776434/149‑1 (MQ=255)
           aCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATgg                                                                                                                                      >  1:841514/1‑149 (MQ=255)
           aCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATgg                                                                                                                                      >  2:3793398/1‑149 (MQ=255)
             cgccgcCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTa                                                                                                                                    <  2:4009709/149‑1 (MQ=255)
             cgccgcCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTa                                                                                                                                    <  2:3351971/149‑1 (MQ=255)
             cgccgcCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTa                                                                                                                                    <  1:2342915/149‑1 (MQ=255)
              gccgccCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTAt                                                                                                                                   <  2:3834882/149‑1 (MQ=255)
                 gccCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGt                                                                                                                                >  2:2188915/1‑149 (MQ=255)
                 cccccccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGt                                                                                                                                <  1:620243/148‑1 (MQ=255)
                  ccccccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGtt                                                                                                                               <  2:4283169/149‑1 (MQ=255)
                        gCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAg                                                                                                                         >  2:2982305/1‑149 (MQ=255)
                         cAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGt                                                                                                                        <  1:1649906/149‑1 (MQ=255)
                          aGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTc                                                                                                                       <  2:2847857/149‑1 (MQ=255)
                          aGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTc                                                                                                                       <  1:213266/149‑1 (MQ=255)
                           gAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTcc                                                                                                                      >  1:3611077/1‑149 (MQ=255)
                                aTTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCcaacaa                                                                                                                 >  1:4202436/1‑149 (MQ=255)
                                aTTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCcaacaa                                                                                                                 <  1:545772/149‑1 (MQ=255)
                                  tCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACAGGTGAAACTGGTCTGGTTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaata                                                                                                               >  2:2404422/1‑149 (MQ=255)
                                    aCTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaataat                                                                                                             >  1:2055575/1‑149 (MQ=255)
                                    aCTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaataat                                                                                                             >  2:3110943/1‑149 (MQ=255)
                                    aCTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaataat                                                                                                             <  1:2796022/149‑1 (MQ=255)
                                    aCTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTATGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAAAGTAAGTTTAATTTTATTGTATCGTTATAAAGTCAAACaataat                                                                                                             >  2:2346683/1‑149 (MQ=255)
                                     cTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAataata                                                                                                            <  1:2967200/149‑1 (MQ=255)
                                          gCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGa                                                                                                       <  1:1447659/149‑1 (MQ=255)
                                          gCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGa                                                                                                       <  1:3837115/149‑1 (MQ=255)
                                          gCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGa                                                                                                       >  1:999189/1‑149 (MQ=255)
                                           cATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGAc                                                                                                      <  1:2281547/149‑1 (MQ=255)
                                             ttACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACgg                                                                                                    <  2:3621011/149‑1 (MQ=255)
                                             ttACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACgg                                                                                                    <  2:716011/149‑1 (MQ=255)
                                                cTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCt                                                                                                 >  1:3990854/1‑149 (MQ=255)
                                                 tGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTc                                                                                                >  2:2113116/1‑149 (MQ=255)
                                                           tgtCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGCCGGGCTCATAGGGGTGa                                                                                      <  1:2416633/149‑1 (MQ=255)
                                                           tgtCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGa                                                                                      <  1:458500/149‑1 (MQ=255)
                                                           tgtCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGa                                                                                      <  2:1180912/149‑1 (MQ=255)
                                                              cACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATACGACGGGCTCATAGGGGTGATAg                                                                                   >  2:4540090/1‑149 (MQ=255)
                                                               aCCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGt                                                                                  >  2:358688/1‑149 (MQ=255)
                                                               aCCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGt                                                                                  >  1:4631917/1‑149 (MQ=255)
                                                                 cGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAt                                                                                <  2:2567963/149‑1 (MQ=255)
                                                                  gTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAtt                                                                               >  2:2008019/1‑149 (MQ=255)
                                                                  gTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAtt                                                                               >  1:1052055/1‑149 (MQ=255)
                                                                   tAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAttt                                                                              >  1:4311077/1‑149 (MQ=255)
                                                                   tAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAttt                                                                              <  1:3117161/149‑1 (MQ=255)
                                                                   tAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAttt                                                                              >  1:2203316/1‑149 (MQ=255)
                                                                   tAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAttt                                                                              >  2:1362897/1‑149 (MQ=255)
                                                                    aaCTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTg                                                                             >  2:601475/1‑149 (MQ=255)
                                                                          aTAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTa                                                                       >  1:763625/1‑149 (MQ=255)
                                                                           tAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTAc                                                                      <  2:3938134/149‑1 (MQ=255)
                                                                           tAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTAc                                                                      <  1:1085392/149‑1 (MQ=255)
                                                                           tAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTAc                                                                      <  1:2574877/149‑1 (MQ=255)
                                                                               cACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCAt                                                                  >  2:1437430/1‑149 (MQ=255)
                                                                               cACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCAt                                                                  >  2:4764597/1‑149 (MQ=255)
                                                                                aCTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATg                                                                 >  2:84340/1‑149 (MQ=255)
                                                                                 cTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGa                                                                >  1:1567485/1‑149 (MQ=255)
                                                                                  tGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGAc                                                               <  1:276919/149‑1 (MQ=255)
                                                                                     tGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGaa                                                            >  2:4229395/1‑149 (MQ=255)
                                                                                        aaCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCa                                                         >  1:3387427/1‑149 (MQ=255)
                                                                                         aCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAt                                                        <  2:3470461/149‑1 (MQ=255)
                                                                                          cTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAtt                                                       >  1:1805513/1‑149 (MQ=255)
                                                                                              tCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaaaa                                                   <  2:384687/149‑1 (MQ=255)
                                                                                               cTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAAt                                                  <  1:416688/149‑1 (MQ=255)
                                                                                               cTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAAt                                                  <  2:4373965/149‑1 (MQ=255)
                                                                                                    tattTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGc                                             <  1:4450447/149‑1 (MQ=255)
                                                                                                      tttGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTc                                           >  1:1774512/1‑149 (MQ=255)
                                                                                                      tttGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTc                                           <  1:381903/149‑1 (MQ=255)
                                                                                                           cAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATg                                      >  2:1558977/1‑149 (MQ=255)
                                                                                                           cAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATg                                      <  2:2628666/149‑1 (MQ=255)
                                                                                                            aaCGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGt                                     <  2:2691827/149‑1 (MQ=255)
                                                                                                               gTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGc                                  <  2:1332842/149‑1 (MQ=255)
                                                                                                                tGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGcc                                 <  1:3557016/149‑1 (MQ=255)
                                                                                                                tGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGcc                                 <  1:1733836/149‑1 (MQ=255)
                                                                                                                tGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATAGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGATCATATGTCGcc                                 >  2:532274/1‑149 (MQ=255)
                                                                                                                  aTGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCAt                               >  1:3252205/1‑149 (MQ=255)
                                                                                                                     ttgttTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTtg                            >  1:2215158/1‑149 (MQ=255)
                                                                                                                      tgttTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTtgt                           <  1:3800226/149‑1 (MQ=255)
                                                                                                                       gttTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTtgtg                          <  1:3185131/149‑1 (MQ=255)
                                                                                                                        tttttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTgtgt                         >  1:1175389/1‑149 (MQ=255)
                                                                                                                        tttttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTgtgt                         <  2:244158/149‑1 (MQ=255)
                                                                                                                         ttttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGtt                        <  1:1277083/149‑1 (MQ=255)
                                                                                                                         ttttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGtt                        <  1:549117/149‑1 (MQ=255)
                                                                                                                           ttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTg                      <  1:958811/149‑1 (MQ=255)
                                                                                                                             aTTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAg                    <  2:4553392/149‑1 (MQ=255)
                                                                                                                                ggTCCATTCAAAATGTAAGTTTAATTTTATTGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTaa                 <  1:3618933/149‑1 (MQ=255)
                                                                                                                                ggTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTaa                 >  1:2456003/1‑149 (MQ=255)
                                                                                                                                ggTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTaa                 >  2:4729751/1‑149 (MQ=255)
                                                                                                                                 gTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAg                <  2:1738518/149‑1 (MQ=255)
                                                                                                                                  tCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGt               >  1:4222706/1‑149 (MQ=255)
                                                                                                                                    cATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGtac             >  1:2915043/1‑147 (MQ=255)
                                                                                                                                    cATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGc             >  2:701848/1‑149 (MQ=255)
                                                                                                                                        cAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaaaa         <  1:4112099/149‑1 (MQ=255)
                                                                                                                                          aaaTGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATc       >  2:4070716/1‑149 (MQ=255)
                                                                                                                                          aaaTGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATc       >  2:3689897/1‑149 (MQ=255)
                                                                                                                                            aTGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATccc     >  1:4015139/1‑149 (MQ=255)
                                                                                                                                             tGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATcccc    >  1:4497902/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCT  >  CP009273/2181499‑2181790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑