Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,223,247 C→T 100% W206* (TGG→TGA)  dusC ← tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,223,2470CT99.0% 1005.9 / ‑0.4 293W206* (TGG→TGA) dusCtRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (137/153);  total (138/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 293 total aligned reads displayed.

TTTTGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTT  >  CP009273/2223101‑2223392
                                                                                                                                                  |                                                                                                                                                 
ttttGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCa                                                                                                                                                 <  1:3743812/149‑1 (MQ=255)
 tttGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAg                                                                                                                                                <  1:4372410/149‑1 (MQ=255)
        caaagcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGa                                                                                                                                         >  1:652141/1‑149 (MQ=255)
         aaagcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGAt                                                                                                                                        <  2:710916/149‑1 (MQ=255)
            gcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTc                                                                                                                                     >  1:2698046/1‑149 (MQ=255)
            gcaaCCACCTCCGGCCACGGCATTCGCGGGTCGGGATCGTTTACCACCCGGCTCAGGGTGGGACTATTGAGCGCCCCGCGACCACGCATCACTGCGTCGCAGCCGCTGAGCGCCATGCATTGTTGCGCGCTCGGTCAGTCCCAGATGTc                                                                                                                                     >  2:392552/1‑149 (MQ=255)
             caaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCa                                                                                                                                    <  2:1866683/149‑1 (MQ=255)
              aaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCAc                                                                                                                                   >  2:4243527/1‑149 (MQ=255)
                ccaccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCg                                                                                                                                 >  2:2832447/1‑149 (MQ=255)
                 caccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGt                                                                                                                                <  2:2548865/149‑1 (MQ=255)
                  accTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGtt                                                                                                                               >  2:455751/1‑149 (MQ=255)
                     tCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGc                                                                                                                            >  2:1146409/1‑149 (MQ=255)
                         gCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATc                                                                                                                        >  1:3516031/1‑149 (MQ=255)
                          ccACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCa                                                                                                                       <  2:4582700/149‑1 (MQ=255)
                            aCGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCAcc                                                                                                                     >  2:611029/1‑149 (MQ=255)
                               gCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGa                                                                                                                  >  2:1976079/1‑149 (MQ=255)
                                 aTTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAat                                                                                                                <  2:3470410/149‑1 (MQ=255)
                                 aTTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAat                                                                                                                <  2:3745290/149‑1 (MQ=255)
                                 aTTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAat                                                                                                                >  1:2994518/1‑149 (MQ=255)
                                 aTTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGATGGGACTATTGACCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATAGTTGCGCGCTCTGTCAGTCCCAGAATTCACCGTTGGCAATCACCGGact                                                                                                                >  1:1040631/1‑147 (MQ=255)
                                  ttCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAata                                                                                                               >  2:3027827/1‑149 (MQ=255)
                                  ttCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAata                                                                                                               >  2:4342271/1‑149 (MQ=255)
                                  ttCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAata                                                                                                               >  2:4368752/1‑149 (MQ=255)
                                   tCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAatat                                                                                                              >  1:2846504/1‑149 (MQ=255)
                                     gcgGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTc                                                                                                            <  1:2373318/149‑1 (MQ=255)
                                       ggTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAg                                                                                                          <  1:3660603/149‑1 (MQ=255)
                                               atatTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGc                                                                                                  >  1:4667660/1‑149 (MQ=255)
                                                     tACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAatat                                                                                            >  1:4053451/1‑149 (MQ=255)
                                                      accaccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATc                                                                                           <  1:108324/149‑1 (MQ=255)
                                                      accaccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATc                                                                                           >  1:2826738/1‑149 (MQ=255)
                                                      accaccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATc                                                                                           >  2:62616/1‑149 (MQ=255)
                                                         accCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGcc                                                                                        >  2:2403547/1‑149 (MQ=255)
                                                           ccGGCTCAGGTTGGGACTATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCTTTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGACAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCaa                                                                                      <  1:4523847/149‑1 (MQ=255)
                                                           ccGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCaa                                                                                      <  1:135906/149‑1 (MQ=255)
                                                             ggCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATc                                                                                    <  2:3299132/149‑1 (MQ=255)
                                                             ggCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATc                                                                                    >  1:1585888/1‑149 (MQ=255)
                                                               cTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGCATATCGCCAATCGc                                                                                  >  2:3605286/1‑149 (MQ=255)
                                                               cTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGc                                                                                  >  2:4175913/1‑149 (MQ=255)
                                                                tCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGcc                                                                                 <  2:894164/149‑1 (MQ=255)
                                                                tCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGcc                                                                                 <  1:1259055/149‑1 (MQ=255)
                                                                   ggTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGc                                                                              <  1:1119451/149‑1 (MQ=255)
                                                                    gTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGcc                                                                             <  1:4215737/149‑1 (MQ=255)
                                                                    gTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGcc                                                                             <  1:3389285/149‑1 (MQ=255)
                                                                    gTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGcc                                                                             <  1:1871218/149‑1 (MQ=255)
                                                                    gTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGcc                                                                             <  2:3677955/149‑1 (MQ=255)
                                                                     ttGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCa                                                                            <  1:3987892/149‑1 (MQ=255)
                                                                     ttGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCa                                                                            >  2:4454168/1‑149 (MQ=255)
                                                                        ggAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTc                                                                         <  1:682131/149‑1 (MQ=255)
                                                                           atatTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCCGTCAat                                                                      >  2:3395210/1‑149 (MQ=255)
                                                                           atatTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAat                                                                      >  2:3005496/1‑149 (MQ=255)
                                                                           atatTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAat                                                                      >  1:1460571/1‑149 (MQ=255)
                                                                            tatTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAata                                                                     >  1:1540279/1‑149 (MQ=255)
                                                                            tatTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAata                                                                     <  2:4721936/149‑1 (MQ=255)
                                                                             atTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAatat                                                                    <  2:2696357/149‑1 (MQ=255)
                                                                             atTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAatat                                                                    <  2:235751/149‑1 (MQ=255)
                                                                              ttGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAAACGCCTGCCAGTCAATATg                                                                   >  1:1668875/1‑149 (MQ=255)
                                                                               tGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCTATATGc                                                                  <  1:4766656/149‑1 (MQ=255)
                                                                                gAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCt                                                                 <  1:3289005/149‑1 (MQ=255)
                                                                                 aGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTc                                                                <  2:1608410/149‑1 (MQ=255)
                                                                                  gcgcCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTcc                                                               <  2:2190674/149‑1 (MQ=255)
                                                                                    gcCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCcgc                                                             >  2:4430472/1‑149 (MQ=255)
                                                                                    gcCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCcgc                                                             <  2:3110913/149‑1 (MQ=255)
                                                                                       ccGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCcgcgcg                                                          >  2:2102277/1‑149 (MQ=255)
                                                                                        cgcgACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCgg                                                         <  2:2648555/149‑1 (MQ=255)
                                                                                           gACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTaa                                                      <  1:2159656/149‑1 (MQ=255)
                                                                                           gACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTaa                                                      <  1:1955573/149‑1 (MQ=255)
                                                                                              cAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAccc                                                   <  1:390519/149‑1 (MQ=255)
                                                                                              cAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAccc                                                   <  2:1086817/149‑1 (MQ=255)
                                                                                               aaTCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCt                                                  >  1:4298822/1‑149 (MQ=255)
                                                                                                   atcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGctc                                              <  2:2954048/149‑1 (MQ=255)
                                                                                                   atcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGctc                                              <  2:316477/149‑1 (MQ=255)
                                                                                                    tcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGctct                                             <  2:2837160/149‑1 (MQ=255)
                                                                                                    tcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGctct                                             >  2:851346/1‑149 (MQ=255)
                                                                                                     caCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCtt                                            >  1:266094/1‑149 (MQ=255)
                                                                                                      aCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCttt                                           <  2:2290721/149‑1 (MQ=255)
                                                                                                      aCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCttt                                           >  1:1759541/1‑149 (MQ=255)
                                                                                                       cTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTc                                          <  2:2572010/149‑1 (MQ=255)
                                                                                                        tGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCg                                         <  1:1168920/149‑1 (MQ=255)
                                                                                                        tGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCg                                         >  1:3761405/1‑149 (MQ=255)
                                                                                                        tGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCg                                         >  1:953146/1‑149 (MQ=255)
                                                                                                                 gCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCAt                                >  1:2257514/1‑149 (MQ=255)
                                                                                                                 gCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCAt                                >  2:4307852/1‑149 (MQ=255)
                                                                                                                     cTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGcac                            >  1:4580431/1‑149 (MQ=255)
                                                                                                                             cATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCt                    >  1:414727/1‑149 (MQ=255)
                                                                                                                             cATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCt                    >  2:1423916/1‑149 (MQ=255)
                                                                                                                                gCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCg                 <  1:2447088/149‑1 (MQ=255)
                                                                                                                                 cATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGt                <  1:2833283/149‑1 (MQ=255)
                                                                                                                                  aTTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTa               <  2:1360480/149‑1 (MQ=255)
                                                                                                                                  aTTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTa               >  1:3179501/1‑149 (MQ=255)
                                                                                                                                  aTTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTa               <  2:480141/149‑1 (MQ=255)
                                                                                                                                      ttgCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAgcgc           <  1:3450813/149‑1 (MQ=255)
                                                                                                                                       tgCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGcc          <  2:1707512/149‑1 (MQ=255)
                                                                                                                                       tgCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGcc          <  2:665917/149‑1 (MQ=255)
                                                                                                                                          gcgcTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGc       >  1:2124641/1‑149 (MQ=255)
                                                                                                                                            gcTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCt     <  1:2846849/149‑1 (MQ=255)
                                                                                                                                             ctctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTg    <  2:1949933/149‑1 (MQ=255)
                                                                                                                                             ctctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTg    <  2:3371897/149‑1 (MQ=255)
                                                                                                                                              tctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGt   <  1:3054742/149‑1 (MQ=255)
                                                                                                                                              tctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGt   >  1:1214732/1‑149 (MQ=255)
                                                                                                                                               ctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGtt  <  1:539457/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTTGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTT  >  CP009273/2223101‑2223392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑