Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,225,190 C→T 100% G231G (GGC→GGT)  yohK → LrgB family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,225,1900CT99.6% 927.9 / ‑4.6 267G231G (GGC→GGT) yohKLrgB family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (133/133);  total (134/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 267 total aligned reads displayed.

GCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTT  >  CP009273/2225047‑2225336
                                                                                                                                               |                                                                                                                                                  
     gCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGc                                                                                                                                          <  1:4265591/149‑1 (MQ=255)
       aCGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGa                                                                                                                                        <  1:3160398/149‑1 (MQ=255)
        cGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGAt                                                                                                                                       <  2:1070489/149‑1 (MQ=255)
         gCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATg                                                                                                                                      >  2:2667641/1‑149 (MQ=255)
         gCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATg                                                                                                                                      <  2:102122/149‑1 (MQ=255)
          cccTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGc                                                                                                                                     <  1:4393137/149‑1 (MQ=255)
          cccTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGc                                                                                                                                     >  1:1804450/1‑149 (MQ=255)
           ccTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCg                                                                                                                                    >  2:2550974/1‑149 (MQ=255)
             tCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTc                                                                                                                                  >  1:1616362/1‑149 (MQ=255)
                gTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTcgcg                                                                                                                               >  1:820962/1‑149 (MQ=255)
                     gcgcgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCAttt                                                                                                                          >  1:639708/1‑149 (MQ=255)
                      cgcgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCAtttt                                                                                                                         <  2:1713521/149‑1 (MQ=255)
                        cgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTg                                                                                                                       <  1:4188994/149‑1 (MQ=255)
                             cgcCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgta                                                                                                                  <  2:2493986/149‑1 (MQ=255)
                              gcCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgtat                                                                                                                 <  2:432198/149‑1 (MQ=255)
                               ccGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgtatg                                                                                                                >  1:2238978/1‑149 (MQ=255)
                               ccGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgtatg                                                                                                                >  1:164632/1‑149 (MQ=255)
                                     tGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAc                                                                                                          <  2:3868700/149‑1 (MQ=255)
                                     tGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAc                                                                                                          <  2:1946500/149‑1 (MQ=255)
                                     tGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAc                                                                                                          >  1:4783477/1‑149 (MQ=255)
                                     tGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAc                                                                                                          >  2:1116171/1‑149 (MQ=255)
                                      ggATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAcg                                                                                                         >  1:4578133/1‑149 (MQ=255)
                                         ttATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGt                                                                                                      <  1:101761/149‑1 (MQ=255)
                                          tATCAGGAAGGTGCATTTAGTTCGCTAGCGCGGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGtt                                                                                                     <  2:263447/149‑1 (MQ=255)
                                          tATCAGGAAGGTGCATTTAGTTCGCCAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGtt                                                                                                     <  2:1279050/149‑1 (MQ=255)
                                           aTCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTg                                                                                                    >  1:659395/1‑149 (MQ=255)
                                             cAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCa                                                                                                  >  1:1198909/1‑149 (MQ=255)
                                              aGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAt                                                                                                 <  2:3797490/149‑1 (MQ=255)
                                              aGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAt                                                                                                 <  2:3797423/149‑1 (MQ=255)
                                                gAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaa                                                                                               >  2:1264678/1‑149 (MQ=255)
                                                  aGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaatt                                                                                             >  2:4672421/1‑149 (MQ=255)
                                                     tGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaattaat                                                                                          <  2:1468140/149‑1 (MQ=255)
                                                      gCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATg                                                                                         >  2:139053/1‑149 (MQ=255)
                                                      gCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATg                                                                                         <  2:4480606/149‑1 (MQ=255)
                                                       cATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATga                                                                                        <  1:2722714/149‑1 (MQ=255)
                                                       cATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATga                                                                                        <  1:2722074/149‑1 (MQ=255)
                                                          ttAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGATTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGAt                                                                                     <  2:1689104/149‑1 (MQ=255)
                                                           tAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGAtt                                                                                    <  2:3261087/149‑1 (MQ=255)
                                                             gTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCa                                                                                  <  2:733772/149‑1 (MQ=255)
                                                                 gCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATc                                                                              <  2:25691/149‑1 (MQ=255)
                                                                  cTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATca                                                                             <  2:772550/149‑1 (MQ=255)
                                                                   tAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATcac                                                                            >  2:815673/1‑149 (MQ=255)
                                                                    aGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTTAGATcaca                                                                           <  2:2442541/149‑1 (MQ=255)
                                                                        cTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACAtata                                                                       <  2:1621133/149‑1 (MQ=255)
                                                                          ggTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaaa                                                                     <  1:2551401/149‑1 (MQ=255)
                                                                           gtgtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAg                                                                    <  2:2991575/149‑1 (MQ=255)
                                                                            tgtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGc                                                                   <  2:4226270/149‑1 (MQ=255)
                                                                              ttATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCca                                                                 <  2:537302/149‑1 (MQ=255)
                                                                                 tGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACaa                                                              >  1:84481/1‑149 (MQ=255)
                                                                                  gCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAAc                                                             <  1:1491289/149‑1 (MQ=255)
                                                                                    gggATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACgg                                                           <  1:2743333/149‑1 (MQ=255)
                                                                                      gATAATTACTTCGCTGATCGCACCGTTCCTGTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGt                                                         <  1:3303482/149‑1 (MQ=255)
                                                                                       aTAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGtt                                                        <  1:4657087/149‑1 (MQ=255)
                                                                                        tAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTc                                                       >  2:4602549/1‑149 (MQ=255)
                                                                                         aaTTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCg                                                      >  2:752729/1‑149 (MQ=255)
                                                                                          aTTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGt                                                     >  1:328046/1‑149 (MQ=255)
                                                                                             aCTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTaaa                                                  <  2:1413855/149‑1 (MQ=255)
                                                                                               ttCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACt                                                <  1:1059447/149‑1 (MQ=255)
                                                                                               ttCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACt                                                <  2:3234096/149‑1 (MQ=255)
                                                                                                 cGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGt                                              <  2:2267396/149‑1 (MQ=255)
                                                                                                 cGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGt                                              <  2:3095645/149‑1 (MQ=255)
                                                                                                  gCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGtt                                             <  2:2378503/149‑1 (MQ=255)
                                                                                                  gCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGtt                                             >  1:4686431/1‑149 (MQ=255)
                                                                                                   cTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTTATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGTGTTAGTAAACTGTTa                                            >  2:2921071/1‑149 (MQ=255)
                                                                                                        cGCACCGTTCCTTTTCCCGTTTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTCAACTGTTATCCCa                                       >  1:2052970/1‑149 (MQ=255)
                                                                                                        cGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCa                                       <  2:1229664/149‑1 (MQ=255)
                                                                                                         gCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAt                                      >  1:1657365/1‑149 (MQ=255)
                                                                                                          cACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAtt                                     >  2:2186876/1‑149 (MQ=255)
                                                                                                           aCCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTa                                    <  2:1184551/149‑1 (MQ=255)
                                                                                                           aCCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTa                                    <  2:910493/149‑1 (MQ=255)
                                                                                                            ccGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTAc                                   >  1:566954/1‑149 (MQ=255)
                                                                                                             cggtCCCTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACa                                  <  2:2995231/146‑1 (MQ=255)
                                                                                                               ttCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATg                                >  1:4774950/1‑149 (MQ=255)
                                                                                                                 ccTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGAt                              <  1:1230779/149‑1 (MQ=255)
                                                                                                                 ccTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGAt                              <  1:1954366/149‑1 (MQ=255)
                                                                                                                    tttCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTAt                           >  1:2160496/1‑149 (MQ=255)
                                                                                                                      tCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGa                         >  1:1779481/1‑149 (MQ=255)
                                                                                                                      tCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGa                         >  1:2482919/1‑149 (MQ=255)
                                                                                                                        ccGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAgg                       >  1:3586084/1‑149 (MQ=255)
                                                                                                                         cGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGc                      >  1:2951132/1‑149 (MQ=255)
                                                                                                                          gATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCa                     >  1:3952592/1‑149 (MQ=255)
                                                                                                                           attattCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTAGATGTATGTTTCACTCGTTGAATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCaa                    >  2:520506/1‑149 (MQ=255)
                                                                                                                                tCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCa               <  1:139053/149‑1 (MQ=255)
                                                                                                                                tCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTGTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCa               <  2:4136825/149‑1 (MQ=255)
                                                                                                                                  tGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATg             >  1:963468/1‑149 (MQ=255)
                                                                                                                                   ggCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGc            >  1:4326684/1‑149 (MQ=255)
                                                                                                                                   ggCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGc            >  1:2275298/1‑149 (MQ=255)
                                                                                                                                    gCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCa           >  1:3027074/1‑149 (MQ=255)
                                                                                                                                    gCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCa           >  2:4649685/1‑149 (MQ=255)
                                                                                                                                     cAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCAt          <  1:1866754/149‑1 (MQ=255)
                                                                                                                                      aGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATc         <  1:2619870/149‑1 (MQ=255)
                                                                                                                                      aGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATc         >  1:3390243/1‑149 (MQ=255)
                                                                                                                                       gTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATcc        >  1:4700612/1‑149 (MQ=255)
                                                                                                                                        tAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCa       <  2:4783477/149‑1 (MQ=255)
                                                                                                                                        tAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCa       <  1:1773510/149‑1 (MQ=255)
                                                                                                                                         aaTGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCAc      >  2:2882648/1‑149 (MQ=255)
                                                                                                                                           tGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGt    <  2:4118703/149‑1 (MQ=255)
                                                                                                                                            gggTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGtt   <  2:3722802/149‑1 (MQ=255)
                                                                                                                                            gggTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGtt   <  1:4571231/149‑1 (MQ=255)
                                                                                                                                             ggCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGttt  >  2:2816557/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTT  >  CP009273/2225047‑2225336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑