BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,241,222	T→G	88.1%	I264L (ATT→CTT)	lysP ←	lysine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,241,222	0	T	G	88.1%	813.7 / 57.3	241	I264L (ATT→CTT)	lysP	lysine transporter
Reads supporting (aligned to +/- strand): ref base T (6/23); new base G (104/108); total (110/131)
Fisher's exact test for biased strand distribution p-value = 4.83e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 245 total aligned reads displayed.

ACCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAA  >  CP009273/2241076‑2241366

aCCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGgaagaa                                                                                                                                                <  2:632299/149‑1 (MQ=255)

aCCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGgaagaa                                                                                                                                                <  2:2378842/149‑1 (MQ=255)

 cccGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGaagaat                                                                                                                                               <  1:1714827/149‑1 (MQ=255)

  ccGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATc                                                                                                                                              >  1:631643/1‑149 (MQ=255)

    gCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAg                                                                                                                                            <  2:927882/149‑1 (MQ=255)

          aGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGAt                                                                                                                                      >  1:973451/1‑149 (MQ=255)

          aGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGAt                                                                                                                                      >  2:4605261/1‑149 (MQ=255)

           gCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATa                                                                                                                                     <  1:555837/149‑1 (MQ=255)

             aCCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAAt                                                                                                                                   <  1:756775/149‑1 (MQ=255)

              ccgccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATc                                                                                                                                  >  2:2032846/1‑149 (MQ=255)

              ccgccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATc                                                                                                                                  >  1:3949298/1‑149 (MQ=255)

               cgccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCa                                                                                                                                 <  1:3861719/149‑1 (MQ=255)

                gccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAg                                                                                                                                <  1:2573473/149‑1 (MQ=255)

                gccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAg                                                                                                                                <  1:4214081/149‑1 (MQ=255)

                gccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAg                                                                                                                                <  2:4411583/149‑1 (MQ=255)

                  cgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGa                                                                                                                              <  2:782292/149‑1 (MQ=255)

                    tCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATc                                                                                                                            <  1:2216112/149‑1 (MQ=255)

                      aGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATcgc                                                                                                                          <  1:3625062/149‑1 (MQ=255)

                        aaTAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGa                                                                                                                        <  1:3861346/149‑1 (MQ=255)

                          tAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAac                                                                                                                      <  1:2980194/149‑1 (MQ=255)

                           aaCTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAaca                                                                                                                     <  2:4052109/149‑1 (MQ=255)

                           aaCTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAaca                                                                                                                     >  2:1875569/1‑149 (MQ=255)

                             cTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGACCGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAAcaca                                                                                                                   >  2:2368097/1‑149 (MQ=255)

                             cTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACAGCGTTACGCAGCAGGCTCGGATCGGTGTACGGACGAATCAGGCTGATAATCAGGATCGCGAAcaca                                                                                                                   >  2:4764977/1‑149 (MQ=255)

                                     atcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaa                                                                                                           <  2:4546390/149‑1 (MQ=255)

                                      tcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaac                                                                                                          >  2:753472/1‑149 (MQ=255)

                                      tcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaac                                                                                                          >  2:4422931/1‑149 (MQ=255)

                                       caCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAacaaca                                                                                                         <  2:3679496/149‑1 (MQ=255)

                                       caCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAacaaca                                                                                                         <  1:659835/149‑1 (MQ=255)

                                         ccgccgccgCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAgg                                                                                                       >  2:4649963/1‑149 (MQ=255)

                                            ccgccgcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGAtt                                                                                                    <  1:179088/149‑1 (MQ=255)

                                            ccgccgcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGAtt                                                                                                    <  1:1304229/149‑1 (MQ=255)

                                                    gagagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGaa                                                                                            >  2:469085/1‑149 (MQ=255)

                                                        gCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACAcc                                                                                        <  1:177594/149‑1 (MQ=255)

                                                         cAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCt                                                                                       <  1:2015595/149‑1 (MQ=255)

                                                           gACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGACGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGa                                                                                     >  2:2889138/1‑149 (MQ=255)

                                                           gACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGa                                                                                     <  2:1355909/149‑1 (MQ=255)

                                                               cgcgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTa                                                                                 >  1:1352941/1‑149 (MQ=255)

                                                               cgcgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTa                                                                                 >  1:3320813/1‑149 (MQ=255)

                                                                gcgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTAc                                                                                >  2:4565184/1‑149 (MQ=255)

                                                                gcgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTAc                                                                                >  2:1013245/1‑149 (MQ=255)

                                                                   tGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgc                                                                             >  2:3993415/1‑149 (MQ=255)

                                                                     cTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgcgc                                                                           >  2:1702629/1‑149 (MQ=255)

                                                                      tGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgcgcg                                                                          >  1:3227825/1‑149 (MQ=255)

                                                                       ggAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGt                                                                         <  1:1099417/149‑1 (MQ=255)

                                                                       ggAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGt                                                                         <  1:562425/149‑1 (MQ=255)

                                                                       ggAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGt                                                                         >  2:4288030/1‑149 (MQ=255)

                                                                        gAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTg                                                                        >  1:498267/1‑149 (MQ=255)

                                                                         aaCACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTgg                                                                       <  2:3564405/149‑1 (MQ=255)

                                                                         aaCACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTgg                                                                       >  2:2992881/1‑149 (MQ=255)

                                                                          acacCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGa                                                                      <  2:244454/149‑1 (MQ=255)

                                                                           cacCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGaa                                                                     <  2:2178972/149‑1 (MQ=255)

                                                                            acCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAAt                                                                    <  1:1573381/149‑1 (MQ=255)

                                                                            acCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAAt                                                                    >  1:1529775/1‑149 (MQ=255)

                                                                              cAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGt                                                                  >  1:1667288/1‑149 (MQ=255)

                                                                                   tGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTc                                                             >  1:1821582/1‑149 (MQ=255)

                                                                                    gAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCg                                                            >  1:3921279/1‑149 (MQ=255)

                                                                                    gAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCg                                                            >  1:2550093/1‑149 (MQ=255)

                                                                                    gAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCg                                                            >  2:2292000/1‑149 (MQ=255)

                                                                                            tAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCt                                                    <  1:3554643/149‑1 (MQ=255)

                                                                                            tAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCt                                                    <  1:3554325/149‑1 (MQ=255)

                                                                                             aaCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCtt                                                   <  2:252806/149‑1 (MQ=255)

                                                                                             aaCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTTGAATGTTTTTCGCCGGATCtt                                                   <  2:2659503/149‑1 (MQ=255)

                                                                                               cGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCg                                                 <  2:3140864/149‑1 (MQ=255)

                                                                                                 cTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGa                                               >  1:2012486/1‑149 (MQ=255)

                                                                                                  tGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGAc                                              <  1:2441065/149‑1 (MQ=255)

                                                                                                  tGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGAc                                              <  1:47582/149‑1 (MQ=255)

                                                                                                    aTGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTc                                            <  2:3749943/149‑1 (MQ=255)

                                                                                                      gTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGc                                          <  1:4576398/149‑1 (MQ=255)

                                                                                                          ttAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgc                                      >  2:376326/1‑149 (MQ=255)

                                                                                                            aaCATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgcag                                    >  2:2927916/1‑149 (MQ=255)

                                                                                                             aCATCGTTACGCAGCAGGCTCGGATCGGTGAACGTAAGAATCAGGCTGATAATAAGGAACGCGAACACATAGAAAAACAGGATTCGCCAGAACACCTGACTTACATCGCGAGGAATGTTTTTCGACGGATCTACTGACTCGACTtcagc                                   >  1:1865400/1‑149 (MQ=255)

                                                                                                               aTCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCaa                                 >  1:1882675/1‑149 (MQ=255)

                                                                                                               aTCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCaa                                 >  2:2389229/1‑149 (MQ=255)

                                                                                                               aTCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGAGAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCaa                                 <  2:2476153/149‑1 (MQ=255)

                                                                                                                tCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAt                                >  2:878632/1‑149 (MQ=255)

                                                                                                                tCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAt                                >  1:1045121/1‑149 (MQ=255)

                                                                                                                tCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAt                                >  2:1596686/1‑149 (MQ=255)

                                                                                                                 cGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATa                               <  1:670315/149‑1 (MQ=255)

                                                                                                                  gTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACATGATTCGCCAGAACACCTGACGTTCCGCGAGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATAc                              <  2:4073113/149‑1 (MQ=255)

                                                                                                                  gTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATAc                              <  1:1682760/149‑1 (MQ=255)

                                                                                                                    tACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCg                            >  1:461576/1‑149 (MQ=255)

                                                                                                                       gcagcaGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATc                         <  1:4313366/149‑1 (MQ=255)

                                                                                                                          gcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGc                      >  2:345262/1‑149 (MQ=255)

                                                                                                                          gcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGc                      >  2:565713/1‑149 (MQ=255)

                                                                                                                           cagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCt                     <  1:1029976/149‑1 (MQ=255)

                                                                                                                           cagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCt                     <  2:2297658/149‑1 (MQ=255)

                                                                                                                              gCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCgg                  >  2:1090254/1‑149 (MQ=255)

                                                                                                                               cTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGt                 >  2:2773607/1‑149 (MQ=255)

                                                                                                                                 cGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTc               >  2:3871480/1‑149 (MQ=255)

                                                                                                                                  ggATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTcc              <  2:596842/149‑1 (MQ=255)

                                                                                                                                    aTCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCt            <  2:1607269/149‑1 (MQ=255)

                                                                                                                                         tgtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGaaa       <  2:2550093/149‑1 (MQ=255)

                                                                                                                                          gtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAag      >  2:4377331/1‑149 (MQ=255)

                                                                                                                                            aCGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAagag    <  2:2605422/149‑1 (MQ=255)

                                                                                                                                            aCGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAagag    <  1:2769807/149‑1 (MQ=255)

                                                                                                                                             cGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAgaga   <  1:2622647/149‑1 (MQ=255)

                                                                                                                                             cGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAgaga   <  1:230085/149‑1 (MQ=255)

                                                                                                                                             cGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAgaga   <  1:1951573/149‑1 (MQ=255)

                                                                                                                                              ggAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGaa  <  1:2417439/149‑1 (MQ=255)

ACCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAA  >  CP009273/2241076‑2241366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`