Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,307,391 C→A 7.9% P142Q (CCG→CAG)  rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,307,3910CA 7.9% 1027.3 / 40.7 289P142Q (CCG→CAG) rcsDphosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand):  ref base C (127/137);  new base A (13/10);  total (140/149)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 298 total aligned reads displayed.

ACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAA  >  CP009273/2307243‑2307538
                                                                                                                                                    |                                                                                                                                                   
    gcCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTgg                                                                                                                                                 <  1:4388658/149‑1 (MQ=255)
    gcCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTgg                                                                                                                                                 >  1:3890550/1‑149 (MQ=255)
       tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCg                                                                                                                                              >  1:77391/1‑149 (MQ=255)
        gAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGa                                                                                                                                             >  2:4293639/1‑149 (MQ=255)
         aaaCAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGAt                                                                                                                                            <  1:1391521/149‑1 (MQ=255)
         aaaCAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGAt                                                                                                                                            <  1:457156/149‑1 (MQ=255)
           aCAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGt                                                                                                                                          <  2:4115873/149‑1 (MQ=255)
             aGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTAt                                                                                                                                        >  1:1016190/1‑149 (MQ=255)
              ggATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTAtt                                                                                                                                       <  1:1292728/149‑1 (MQ=255)
              ggATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTAtt                                                                                                                                       <  1:369609/149‑1 (MQ=255)
               gATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTa                                                                                                                                      <  1:4631313/149‑1 (MQ=255)
               gATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACAGTGGTCGATGTATTa                                                                                                                                      >  1:3775669/1‑149 (MQ=255)
                  gTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCt                                                                                                                                   >  1:3923480/1‑149 (MQ=255)
                  gTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCt                                                                                                                                   <  2:4232929/149‑1 (MQ=255)
                    ctactaTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGa                                                                                                                                 >  1:1628928/1‑149 (MQ=255)
                      actaTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAAt                                                                                                                               <  1:2165006/149‑1 (MQ=255)
                       ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATg                                                                                                                              <  2:2743920/149‑1 (MQ=255)
                        taTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATgg                                                                                                                             >  2:3129870/1‑149 (MQ=255)
                          tCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACAGTGGTCGATGTATTACCTGAATGGTc                                                                                                                           >  2:2046079/1‑149 (MQ=255)
                           cTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCa                                                                                                                          <  2:2871853/149‑1 (MQ=255)
                              gagaAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGa                                                                                                                       >  1:2936674/1‑149 (MQ=255)
                               agaAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGAt                                                                                                                      >  2:1648582/1‑149 (MQ=255)
                               agaAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGAGACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGAt                                                                                                                      >  1:4567816/1‑149 (MQ=255)
                                 aaaCCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATaa                                                                                                                    <  2:3687859/149‑1 (MQ=255)
                                  aaCCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACAGTGGTCGATGTATTACCTGAATGGTCAGGATAAc                                                                                                                   <  2:4460424/149‑1 (MQ=255)
                                   aCCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACa                                                                                                                  >  1:4672445/1‑149 (MQ=255)
                                   aCCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACAGTGGTCGATGTATTACCTGAATGGTCAGGATAACa                                                                                                                  >  1:937206/1‑149 (MQ=255)
                                   aCCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGAGGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACAGTGGTCGATGTATTACCTGAATGGTCAGGATAACa                                                                                                                  >  1:937236/1‑149 (MQ=255)
                                      aCGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTc                                                                                                               >  1:2397673/1‑149 (MQ=255)
                                       cgccgcAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCt                                                                                                              <  1:2096787/149‑1 (MQ=255)
                                          cgcAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGt                                                                                                           <  2:3695614/149‑1 (MQ=255)
                                          cgcAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGt                                                                                                           <  1:1481465/149‑1 (MQ=255)
                                           gcAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTg                                                                                                          >  1:832141/1‑149 (MQ=255)
                                             aaaaCGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCt                                                                                                        <  2:121580/149‑1 (MQ=255)
                                             aaaaCGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCt                                                                                                        <  1:155539/149‑1 (MQ=255)
                                              aaaCGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTg                                                                                                       >  2:3191788/1‑149 (MQ=255)
                                                aCGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGAt                                                                                                     >  1:2299852/1‑149 (MQ=255)
                                                 cGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGAtc                                                                                                    <  1:4609845/149‑1 (MQ=255)
                                                  ggAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGAtct                                                                                                   >  1:2985060/1‑149 (MQ=255)
                                                  ggAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGAtct                                                                                                   >  1:3274528/1‑149 (MQ=255)
                                                  ggAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGAtct                                                                                                   >  2:818400/1‑149 (MQ=255)
                                                   gAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGAtctc                                                                                                  >  2:2420013/1‑149 (MQ=255)
                                                      gCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAAc                                                                                               <  1:3975173/149‑1 (MQ=255)
                                                       cGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAAcc                                                                                              >  1:1597564/1‑149 (MQ=255)
                                                        gTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAAccc                                                                                             >  1:4548629/1‑149 (MQ=255)
                                                             tCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTAccc                                                                                        <  2:2710965/149‑1 (MQ=255)
                                                                   gCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTaaa                                                                                  <  2:2937766/149‑1 (MQ=255)
                                                                   gCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTaaa                                                                                  <  2:877333/149‑1 (MQ=255)
                                                                       tcACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGAtc                                                                              >  2:1575174/1‑149 (MQ=255)
                                                                       tcACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGAtc                                                                              >  2:2152390/1‑149 (MQ=255)
                                                                        cACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGAtct                                                                             <  1:562696/149‑1 (MQ=255)
                                                                        cACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGAtct                                                                             >  2:4174997/1‑149 (MQ=255)
                                                                         aCGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGAtctc                                                                            >  1:92556/1‑149 (MQ=255)
                                                                         aCGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGAtctc                                                                            >  2:3764254/1‑149 (MQ=255)
                                                                          cGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCa                                                                           <  2:194586/149‑1 (MQ=255)
                                                                          cGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCa                                                                           <  1:2019907/149‑1 (MQ=255)
                                                                           gACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCAc                                                                          <  1:2816563/149‑1 (MQ=255)
                                                                            aCAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCAcc                                                                         <  2:2334513/149‑1 (MQ=255)
                                                                                     cGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGAttt                                                                <  1:3651037/149‑1 (MQ=255)
                                                                                        ttGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACAGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTaaa                                                             >  1:1053778/1‑149 (MQ=255)
                                                                                         tGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAg                                                            <  2:3710246/149‑1 (MQ=255)
                                                                                          gagaTGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCACAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTaaata                                                           >  2:2171965/1‑147 (MQ=255)
                                                                                                  tCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCg                                                   >  2:4323463/1‑149 (MQ=255)
                                                                                                    aGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTc                                                 >  1:4210922/1‑149 (MQ=255)
                                                                                                     gCGGATGGCCACTTATCTGGATACATTGTGGGGCGCAGAAATTGTACCGTGGTCGATGTATTACCTGAATGGTCAGTATAACATTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCa                                                <  1:2527297/149‑1 (MQ=255)
                                                                                                      cGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAg                                               <  2:3425309/149‑1 (MQ=255)
                                                                                                        gATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCg                                             >  2:2518061/1‑149 (MQ=255)
                                                                                                         aTGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGa                                            <  2:3956074/149‑1 (MQ=255)
                                                                                                         aTGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGa                                            <  2:1056096/149‑1 (MQ=255)
                                                                                                             ccACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACAtt                                        <  1:1872548/149‑1 (MQ=255)
                                                                                                             ccACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACAtt                                        <  2:3837777/149‑1 (MQ=255)
                                                                                                             ccACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACAtt                                        >  1:2472914/1‑149 (MQ=255)
                                                                                                              cACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACAttg                                       >  1:1949906/1‑149 (MQ=255)
                                                                                                               aCTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACAttgt                                      >  1:2688128/1‑149 (MQ=255)
                                                                                                               aCTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACAttgt                                      <  2:1469761/149‑1 (MQ=255)
                                                                                                                    tCTGGATACTTTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAACGATCTCACCTCCGGATTTAACGAATCGACCGTCAGCGACATTGTTGAtt                                 <  1:3465682/149‑1 (MQ=255)
                                                                                                                      tGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCa                               >  2:3244968/1‑149 (MQ=255)
                                                                                                                       ggATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAc                              <  2:2619686/149‑1 (MQ=255)
                                                                                                                        gATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcg                             <  1:287344/149‑1 (MQ=255)
                                                                                                                         aTACATTGTGGGGCGCATAAATTGTACGGTGGTCGATGTATTACCTGAATGGTCTGGATAACTGTCTGGTGCTGATCTCAACCCTCCCCCTCAACGATCTCACCTCCGGATTTAAACACTCGACCGTCAGCGACATTGTTGATTCAcgt                            <  1:2047803/149‑1 (MQ=255)
                                                                                                                         aTACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcgt                            >  1:1901651/1‑149 (MQ=255)
                                                                                                                          tACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcgtc                           >  1:3997610/1‑149 (MQ=255)
                                                                                                                          tACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcgtc                           >  1:3361894/1‑149 (MQ=255)
                                                                                                                          tACAGTGTGGGGCGCAGAAAATGTCCAGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcgtc                           <  2:2813942/149‑1 (MQ=255)
                                                                                                                            cATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcgtcgt                         <  2:4374056/149‑1 (MQ=255)
                                                                                                                            cATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAAAGGTCAGGAGAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcgtcgt                         <  2:2767698/149‑1 (MQ=255)
                                                                                                                             aTTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAATGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTg                        >  2:697638/1‑149 (MQ=255)
                                                                                                                                  ggggCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCagag                   <  2:523576/149‑1 (MQ=255)
                                                                                                                                   gggCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAgaga                  <  2:3623565/149‑1 (MQ=255)
                                                                                                                                   gggCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAgaga                  >  1:2590302/1‑149 (MQ=255)
                                                                                                                                   gggCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAgaga                  <  2:1873755/149‑1 (MQ=255)
                                                                                                                                   gggCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTGAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAgaga                  <  2:3623712/149‑1 (MQ=255)
                                                                                                                                       gcAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGtt              >  1:928233/1‑149 (MQ=255)
                                                                                                                                          gAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCCGAGATGTTGCa           >  1:4129537/1‑149 (MQ=255)
                                                                                                                                          gAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCa           >  2:1265490/1‑149 (MQ=255)
                                                                                                                                            aaaTGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAAc         >  2:1149524/1‑149 (MQ=255)
                                                                                                                                               tGTACAGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAgg      >  1:3617400/1‑149 (MQ=255)
                                                                                                                                                 tACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGcc    >  1:2731432/1‑149 (MQ=255)
                                                                                                                                                   ccGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCaa  <  2:4704563/149‑1 (MQ=255)
                                                                                                                                                   ccGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCaa  >  2:1343958/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAA  >  CP009273/2307243‑2307538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑