Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,352,573 C→A 100% P353P (CCG→CCT)  rhmT ← putative L‑rhamnonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,352,5730CA99.6% 956.6 / ‑4.7 276P353P (CCG→CCT) rhmTputative L‑rhamnonate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (139/136);  minor base T (0/1);  total (139/137)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 276 total aligned reads displayed.

CCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCC  >  CP009273/2352428‑2352718
                                                                                                                                                 |                                                                                                                                                 
ccAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGc                                                                                                                                                <  1:3939265/149‑1 (MQ=255)
  aGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCAt                                                                                                                                              >  1:1537440/1‑149 (MQ=255)
   gCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATc                                                                                                                                             <  2:1535423/149‑1 (MQ=255)
    cGTCAGTACTCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCCCGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAATCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCg                                                                                                                                            <  1:4121885/149‑1 (MQ=255)
       cAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTcc                                                                                                                                         >  1:3748061/1‑149 (MQ=255)
        aGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCa                                                                                                                                        <  1:1287795/149‑1 (MQ=255)
            aTCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCGGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAac                                                                                                                                    <  2:3369582/149‑1 (MQ=255)
               ccgccgCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACt                                                                                                                                 <  1:267264/149‑1 (MQ=255)
                    gcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTg                                                                                                                            >  2:2080590/1‑149 (MQ=255)
                     cATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGc                                                                                                                           <  2:1605304/149‑1 (MQ=255)
                      aTCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCa                                                                                                                          >  2:1255911/1‑149 (MQ=255)
                       tCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCAc                                                                                                                         >  1:1013059/1‑149 (MQ=255)
                       tCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCAc                                                                                                                         <  1:4470339/149‑1 (MQ=255)
                            ggCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCaa                                                                                                                    >  2:2873154/1‑149 (MQ=255)
                              cAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATa                                                                                                                  <  1:404537/149‑1 (MQ=255)
                               aaaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATaa                                                                                                                 >  1:1696576/1‑149 (MQ=255)
                                aaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATaaa                                                                                                                >  1:748287/1‑149 (MQ=255)
                                aaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATaaa                                                                                                                >  1:1258996/1‑149 (MQ=255)
                                 aTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAAc                                                                                                               <  2:2025150/149‑1 (MQ=255)
                                 aTAACGTGTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAAc                                                                                                               <  2:1725991/149‑1 (MQ=255)
                                 aTAACGTATCTGATTTCACGCGCAGGAACGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCACGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGACCAGAACACTGGCTGCACGGCAATAAAc                                                                                                               >  1:1825748/1‑149 (MQ=255)
                                  tAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAAcc                                                                                                              >  1:1184029/1‑149 (MQ=255)
                                  tAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAAcc                                                                                                              <  2:1676237/149‑1 (MQ=255)
                                       tttCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATa                                                                                                         >  1:3708903/1‑149 (MQ=255)
                                       tttCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATa                                                                                                         >  1:1768795/1‑149 (MQ=255)
                                        ttCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAg                                                                                                        >  2:2997992/1‑149 (MQ=255)
                                          cTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCt                                                                                                      >  1:4220499/1‑149 (MQ=255)
                                           tGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAACACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTg                                                                                                     <  1:2737288/149‑1 (MQ=255)
                                            gCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGc                                                                                                    >  1:896858/1‑149 (MQ=255)
                                            gCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTACCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGc                                                                                                    <  2:693519/149‑1 (MQ=255)
                                                  aCGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacaca                                                                                              >  1:1603379/1‑149 (MQ=255)
                                                  aCGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacaca                                                                                              >  2:2731720/1‑149 (MQ=255)
                                                         ggATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCt                                                                                       >  1:3694193/1‑149 (MQ=255)
                                                         ggATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCt                                                                                       <  1:1476540/149‑1 (MQ=255)
                                                            tCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACg                                                                                    >  2:1608699/1‑149 (MQ=255)
                                                            tCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACg                                                                                    >  2:4325218/1‑149 (MQ=255)
                                                              ggggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGAt                                                                                  <  1:4284403/149‑1 (MQ=255)
                                                              ggggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGAt                                                                                  <  2:148366/149‑1 (MQ=255)
                                                              ggggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGAt                                                                                  <  2:2110660/149‑1 (MQ=255)
                                                              ggggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGAt                                                                                  <  1:180290/149‑1 (MQ=255)
                                                              ggggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGAt                                                                                  >  1:1988783/1‑149 (MQ=255)
                                                               gggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATc                                                                                 >  2:4165272/1‑149 (MQ=255)
                                                               gggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATc                                                                                 <  2:3126805/149‑1 (MQ=255)
                                                               gggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATc                                                                                 <  2:1161693/149‑1 (MQ=255)
                                                                 gCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGc                                                                               >  2:1937730/1‑149 (MQ=255)
                                                                  cAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGcc                                                                              <  1:332355/149‑1 (MQ=255)
                                                                  cAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGcc                                                                              <  1:4347145/149‑1 (MQ=255)
                                                                    aTAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCAt                                                                            <  1:4666067/149‑1 (MQ=255)
                                                                    aTAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCAt                                                                            >  2:4162211/1‑149 (MQ=255)
                                                                    aTAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCAt                                                                            <  1:21454/149‑1 (MQ=255)
                                                                    aTAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCAt                                                                            <  1:2953971/149‑1 (MQ=255)
                                                                     tAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCAtt                                                                           >  1:350884/1‑149 (MQ=255)
                                                                      aaaCCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTa                                                                          >  1:4369241/1‑149 (MQ=255)
                                                                       aaCCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTAc                                                                         >  1:3788809/1‑149 (MQ=255)
                                                                       aaCCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTAc                                                                         >  2:2795941/1‑149 (MQ=255)
                                                                       aaCCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTAc                                                                         >  2:1417803/1‑149 (MQ=255)
                                                                         cccGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTg                                                                       >  1:3105314/1‑149 (MQ=255)
                                                                          ccGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTgg                                                                      >  2:4463747/1‑149 (MQ=255)
                                                                                      ccAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGccc                                                          >  2:2610904/1‑149 (MQ=255)
                                                                                        aaaCAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGCAAGCAGCCCgg                                                        >  2:3287458/1‑149 (MQ=255)
                                                                                         aaCAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGa                                                       >  2:3076265/1‑149 (MQ=255)
                                                                                         aaCAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGa                                                       <  1:3584091/149‑1 (MQ=255)
                                                                                              gTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGac                                                  <  1:2077045/149‑1 (MQ=255)
                                                                                               tttACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGacc                                                 >  1:3994043/1‑149 (MQ=255)
                                                                                               tttACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGacc                                                 >  2:3225911/1‑149 (MQ=255)
                                                                                                  aCAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaat                                              >  2:3715309/1‑149 (MQ=255)
                                                                                                   cAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaata                                             <  1:441238/149‑1 (MQ=255)
                                                                                                    aaaaCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaatac                                            <  1:29915/149‑1 (MQ=255)
                                                                                                      aaCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaatacca                                          >  1:1391822/1‑149 (MQ=255)
                                                                                                       aCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaa                                         <  2:2390606/149‑1 (MQ=255)
                                                                                                       aCCAAGTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaa                                         <  2:2658846/149‑1 (MQ=255)
                                                                                                           aTTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGcc                                     >  2:820682/1‑149 (MQ=255)
                                                                                                                  cagccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCgccgcc                              <  1:4555278/149‑1 (MQ=255)
                                                                                                                   agccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCTACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCa                             <  2:493351/149‑1 (MQ=255)
                                                                                                                     ccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGt                           <  1:1383770/149‑1 (MQ=255)
                                                                                                                     ccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGt                           <  1:2894032/149‑1 (MQ=255)
                                                                                                                     ccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGt                           >  1:3136229/1‑149 (MQ=255)
                                                                                                                        gcgcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAAt                        <  1:3561795/149‑1 (MQ=255)
                                                                                                                         cgcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATg                       <  2:3434149/149‑1 (MQ=255)
                                                                                                                          gcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGt                      >  2:2906336/1‑149 (MQ=255)
                                                                                                                          gcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCAGCCGCCAGTAATGt                      >  1:667943/1‑149 (MQ=255)
                                                                                                                             gTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                   >  2:2447626/1‑149 (MQ=255)
                                                                                                                             gTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                   >  2:517562/1‑149 (MQ=255)
                                                                                                                             gTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                   <  2:4711525/149‑1 (MQ=255)
                                                                                                                             gTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                   <  1:3268792/149‑1 (MQ=255)
                                                                                                                             gTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                   <  1:567647/149‑1 (MQ=255)
                                                                                                                             gTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                   >  1:751850/1‑149 (MQ=255)
                                                                                                                             gTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                   <  2:1835061/149‑1 (MQ=255)
                                                                                                                              tACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAgc                  <  1:30370/149‑1 (MQ=255)
                                                                                                                                  ggAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTg              >  1:3862850/1‑149 (MQ=255)
                                                                                                                                   gAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGc             <  1:2504516/149‑1 (MQ=255)
                                                                                                                                     aaGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGa           >  2:1889679/1‑149 (MQ=255)
                                                                                                                                       gCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACCCAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACa         <  2:3191882/149‑1 (MQ=255)
                                                                                                                                       gCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACa         >  1:2258124/1‑149 (MQ=255)
                                                                                                                                        cAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAAGGCCCGCCGCCAGTAATGTCAGCGCTGCGACAt        <  2:3857822/149‑1 (MQ=255)
                                                                                                                                         aGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACAtt       <  1:3729627/149‑1 (MQ=255)
                                                                                                                                         aGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACAtt       <  2:1284910/149‑1 (MQ=255)
                                                                                                                                          gTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTa      <  2:4093489/149‑1 (MQ=255)
                                                                                                                                          gTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTa      <  1:102611/149‑1 (MQ=255)
                                                                                                                                              tgtAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGcc  <  1:2628978/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCC  >  CP009273/2352428‑2352718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑