BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,403,485	C→A	6.9%	L449L (CTG→CTT)	yfbS ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,403,485	0	C	A	6.9%	1095.3 / 30.9	289	L449L (CTG→CTT)	yfbS	putative transporter
Reads supporting (aligned to +/- strand): ref base C (147/122); new base A (9/11); total (156/133)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 300 total aligned reads displayed.

TCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAGCGCTA  >  CP009273/2403339‑2403633

  cATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATcagca                                                                                                                                                  >  1:2995879/1‑149 (MQ=255)

  cATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATaagca                                                                                                                                                  <  1:551357/149‑1 (MQ=255)

     cAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATACTGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAgcaggc                                                                                                                                               <  2:4510984/149‑1 (MQ=255)

      aGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAgcaggca                                                                                                                                              <  1:2095670/149‑1 (MQ=255)

        cccTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGcaggcagg                                                                                                                                            <  1:3065513/149‑1 (MQ=255)

         ccTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCaggcaggc                                                                                                                                           <  1:3419016/149‑1 (MQ=255)

          cTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCg                                                                                                                                          >  1:2399745/1‑149 (MQ=255)

                ccGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATa                                                                                                                                    >  2:4403900/1‑149 (MQ=255)

                ccGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATa                                                                                                                                    <  2:3315444/149‑1 (MQ=255)

                 cGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAg                                                                                                                                   <  2:856017/149‑1 (MQ=255)

                 cGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAg                                                                                                                                   <  1:4758763/149‑1 (MQ=255)

                  gCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGc                                                                                                                                  <  1:4245694/149‑1 (MQ=255)

                    cAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCgg                                                                                                                                <  1:751382/149‑1 (MQ=255)

                         cgACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACa                                                                                                                           <  2:4672965/149‑1 (MQ=255)

                          gACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAg                                                                                                                          <  1:4333202/149‑1 (MQ=255)

                          gACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAg                                                                                                                          <  2:4184640/149‑1 (MQ=255)

                          gACACCGCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAg                                                                                                                          <  1:2585114/149‑1 (MQ=255)

                            cacCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGGATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGa                                                                                                                        >  1:3908725/1‑149 (MQ=255)

                            cacCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGa                                                                                                                        >  1:3030156/1‑149 (MQ=255)

                            cacCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGa                                                                                                                        <  1:3824258/149‑1 (MQ=255)

                             acCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGAt                                                                                                                       <  2:1201308/149‑1 (MQ=255)

                             acCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAAGACTGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGAt                                                                                                                       <  2:3958932/149‑1 (MQ=255)

                               cTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTa                                                                                                                     <  2:480636/149‑1 (MQ=255)

                                    gTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAAt                                                                                                                <  1:1431979/149‑1 (MQ=255)

                                    gTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGAGTAGGAAt                                                                                                                >  1:2907846/1‑149 (MQ=255)

                                     ttttCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAAtt                                                                                                               <  1:4428234/149‑1 (MQ=255)

                                     ttttCTGTAATGCCACAGAAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAAtt                                                                                                               <  1:3220266/149‑1 (MQ=255)

                                      tttCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAAttt                                                                                                              >  2:2484215/1‑149 (MQ=255)

                                          tGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCTATGATAGCGGCAACAGGATTAGGAATTTCATc                                                                                                          <  2:4291895/149‑1 (MQ=255)

                                           gTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCt                                                                                                         >  1:1934234/1‑149 (MQ=255)

                                            tAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTg                                                                                                        >  1:2242712/1‑149 (MQ=255)

                                                   acaGCAAACGGCATCATCCCCACGATCACAATACTGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCAt                                                                                                 <  2:2873143/149‑1 (MQ=255)

                                                   acaGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCACCAGGATTAGGAATTTCATCTGTCAGCAt                                                                                                 >  2:3291224/1‑149 (MQ=255)

                                                   aaaGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTACACATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCAt                                                                                                 >  1:3667667/3‑149 (MQ=255)

                                                     aGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTa                                                                                               >  1:17615/1‑149 (MQ=255)

                                                       cAAACGTCATCATCCCAACGATCTAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATTCTGCGGAATTTCCCAATCAGCAGGCAGTCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAAc                                                                                             <  2:1474115/149‑1 (MQ=255)

                                                        aaaCGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACg                                                                                            >  2:3872866/1‑149 (MQ=255)

                                                          aCGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGcc                                                                                          <  1:2775976/149‑1 (MQ=255)

                                                          aCGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGcc                                                                                          <  2:2731620/149‑1 (MQ=255)

                                                           cGGCATCATCCCAACGATCAAAATAAGGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGcca                                                                                         <  1:1921594/149‑1 (MQ=255)

                                                              catcatCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACATCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGccacca                                                                                      <  1:684435/149‑1 (MQ=255)

                                                              catcatCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGccacca                                                                                      <  1:63960/149‑1 (MQ=255)

                                                               atcatcCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGACTTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCAt                                                                                     >  2:3659637/1‑149 (MQ=255)

                                                               atcatcCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCAt                                                                                     >  2:1268676/1‑149 (MQ=255)

                                                                 catcCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTa                                                                                   <  1:2871891/149‑1 (MQ=255)

                                                                     ccAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCAc                                                                               >  1:3865692/1‑149 (MQ=255)

                                                                          gATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACcagac                                                                          <  1:2699001/149‑1 (MQ=255)

                                                                               aaaTAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGaaa                                                                     <  1:998419/149‑1 (MQ=255)

                                                                               aaaTAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCAACATTAGCACCAGACAGaaa                                                                     >  1:4728614/1‑149 (MQ=255)

                                                                                aataatGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGaaaa                                                                    >  1:1474760/1‑149 (MQ=255)

                                                                                aataatGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGaaaa                                                                    >  2:728786/1‑149 (MQ=255)

                                                                                 ataatGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAAt                                                                   >  1:889294/1‑149 (MQ=255)

                                                                                 ataatGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAAt                                                                   >  1:1424171/1‑149 (MQ=255)

                                                                                    atGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCATGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGc                                                                <  2:2918671/149‑1 (MQ=255)

                                                                                     tGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCa                                                               >  2:512861/1‑149 (MQ=255)

                                                                                     tGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCa                                                               >  2:513494/1‑149 (MQ=255)

                                                                                       cTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATg                                                             <  2:3092421/149‑1 (MQ=255)

                                                                                        tCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATgg                                                            <  2:2687667/149‑1 (MQ=255)

                                                                                        tCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATgg                                                            <  1:2303280/149‑1 (MQ=255)

                                                                                         cGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATCGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATggg                                                           >  2:4118528/1‑149 (MQ=255)

                                                                                          ggCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATgggg                                                          <  1:459117/149‑1 (MQ=255)

                                                                                           tccAGTGTTTGTATTTATAGGCGTTTTCAGATTATATACAGCGGATTTTACCAATATGCAGGCAGGCGATGTTAGCGGAAACTGGATTAGGAATTTCATCTGTCAGCATTAACGGCACCATTTGAAAAAGACAGTTAATGGAATGGGGt                                                         <  2:1184852/148‑1 (MQ=255)

                                                                                              aGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCCTCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGcc                                                      <  2:3494310/149‑1 (MQ=255)

                                                                                              aGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGcc                                                      <  1:4454747/149‑1 (MQ=255)

                                                                                                tGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTg                                                    <  1:4225832/149‑1 (MQ=255)

                                                                                                  aaTGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGc                                                  >  1:4666056/1‑149 (MQ=255)

                                                                                                  aaTGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGc                                                  >  1:1100206/1‑149 (MQ=255)

                                                                                                   aTGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCt                                                 >  1:3550398/1‑149 (MQ=255)

                                                                                                    tGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCtg                                                >  2:2282026/1‑149 (MQ=255)

                                                                                                              ggCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTATCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTg                                      >  1:3344326/1‑149 (MQ=255)

                                                                                                               gcgcTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGa                                     >  2:1813461/1‑149 (MQ=255)

                                                                                                                cgcTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGAt                                    <  1:12635/149‑1 (MQ=255)

                                                                                                                 gcTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATg                                   >  1:1220700/1‑149 (MQ=255)

                                                                                                                 gcTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATg                                   <  2:3208856/149‑1 (MQ=255)

                                                                                                                 gcTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATg                                   >  2:1707020/1‑149 (MQ=255)

                                                                                                                   tttCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCt                                 >  2:304157/1‑149 (MQ=255)

                                                                                                                   tttCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCt                                 >  2:1373914/1‑149 (MQ=255)

                                                                                                                     tCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTtc                               <  2:1528642/149‑1 (MQ=255)

                                                                                                                         cATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTtcactc                           <  2:2479065/149‑1 (MQ=255)

                                                                                                                            cTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCAcc                        >  2:1993998/1‑149 (MQ=255)

                                                                                                                              ataCAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACctc                      >  1:2124146/1‑149 (MQ=255)

                                                                                                                              ataCAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACctc                      >  2:4243059/1‑149 (MQ=255)

                                                                                                                               taCAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACctct                     <  2:4334913/149‑1 (MQ=255)

                                                                                                                               taCAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACctct                     <  1:3724272/149‑1 (MQ=255)

                                                                                                                                  aGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTcc                  >  1:808230/1‑149 (MQ=255)

                                                                                                                                  aGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTcc                  >  1:3259907/1‑149 (MQ=255)

                                                                                                                                  aGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTcc                  <  2:2297002/149‑1 (MQ=255)

                                                                                                                                   gCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCg                 >  2:2105455/1‑149 (MQ=255)

                                                                                                                                    cGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCgg                >  1:3499950/1‑149 (MQ=255)

                                                                                                                                      gAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTa              >  1:4301322/1‑149 (MQ=255)

                                                                                                                                      gAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTa              >  2:3788501/1‑149 (MQ=255)

                                                                                                                                      gAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTa              >  2:1678690/1‑149 (MQ=255)

                                                                                                                                           tCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTc         <  2:263370/149‑1 (MQ=255)

                                                                                                                                           tCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGAACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTc         >  2:192221/1‑149 (MQ=255)

                                                                                                                                            ccccATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCa        >  2:3420612/1‑149 (MQ=255)

                                                                                                                                             cccATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAg       >  1:3680951/1‑149 (MQ=255)

                                                                                                                                             cccATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAg       >  1:1346278/1‑149 (MQ=255)

                                                                                                                                              ccATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAgc      >  1:1689294/1‑149 (MQ=255)

                                                                                                                                                aTCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAgcgc    <  2:3618921/149‑1 (MQ=255)

                                                                                                                                                 tCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGTATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAGCGCt   >  1:2492740/1‑149 (MQ=255)

TCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAGCGCTA  >  CP009273/2403339‑2403633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`