Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 214,084 G→T 87.5% G393G (GGC→GGA)  proS ← prolyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273214,0840GT87.5% 664.4 / 93.5 264G393G (GGC→GGA) proSprolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (18/15);  new base T (126/105);  total (144/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 276 total aligned reads displayed.

ACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAA  >  CP009273/213937‑214229
                                                                                                                                                   |                                                                                                                                                 
aCCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCgc                                                                                                                                                  >  1:2991368/1‑149 (MQ=255)
    aTCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtccagc                                                                                                                                              >  1:2324317/1‑149 (MQ=255)
    aTCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtccagc                                                                                                                                              >  1:442777/1‑149 (MQ=255)
         cAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGccacca                                                                                                                                         <  1:197711/149‑1 (MQ=255)
           ggATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACg                                                                                                                                       >  1:3722067/1‑149 (MQ=255)
           ggATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACg                                                                                                                                       >  1:1332719/1‑149 (MQ=255)
            gATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGt                                                                                                                                      <  1:2555008/149‑1 (MQ=255)
            gATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGt                                                                                                                                      >  1:1834906/1‑149 (MQ=255)
              tttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTa                                                                                                                                    >  1:3119008/1‑149 (MQ=255)
              tttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTa                                                                                                                                    >  2:412872/1‑149 (MQ=255)
                  gTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGa                                                                                                                                >  1:2488734/1‑149 (MQ=255)
                     aCGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGatat                                                                                                                             >  1:3261251/1‑149 (MQ=255)
                      cGGCCATCTTCACCCTGTACGGAGGCTTTCAGTACTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATc                                                                                                                            <  1:2154249/149‑1 (MQ=255)
                       ggCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCt                                                                                                                           <  2:1535565/149‑1 (MQ=255)
                        gCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTg                                                                                                                          <  2:1793641/149‑1 (MQ=255)
                           aTCTTCACCCTGTACGGAGGCTTTCAGAGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTAACGGATATCTGCaa                                                                                                                       >  2:4561742/1‑149 (MQ=255)
                             cTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACt                                                                                                                     >  1:3518855/1‑149 (MQ=255)
                              ttCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACtt                                                                                                                    >  2:4460644/1‑149 (MQ=255)
                              ttCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACtt                                                                                                                    >  2:3494767/1‑149 (MQ=255)
                               tCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTc                                                                                                                   <  1:1458461/149‑1 (MQ=255)
                                cACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTcc                                                                                                                  <  2:570134/149‑1 (MQ=255)
                                cACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTcc                                                                                                                  <  1:28797/149‑1 (MQ=255)
                                   ccTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCggg                                                                                                               <  1:746773/149‑1 (MQ=255)
                                     tGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGt                                                                                                             >  2:3998403/1‑149 (MQ=255)
                                      gTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTa                                                                                                            >  2:356266/1‑149 (MQ=255)
                                       tACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAg                                                                                                           >  1:2234345/1‑149 (MQ=255)
                                        aCGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGc                                                                                                          >  1:1429912/1‑149 (MQ=255)
                                          ggAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGa                                                                                                        >  2:4641680/1‑149 (MQ=255)
                                            aGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAATATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACa                                                                                                      >  1:4560441/1‑149 (MQ=255)
                                              gCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATc                                                                                                    >  1:1556673/1‑149 (MQ=255)
                                               cTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcg                                                                                                   >  1:2902884/1‑149 (MQ=255)
                                               cTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcg                                                                                                   >  2:1223909/1‑149 (MQ=255)
                                                  tCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGa                                                                                                <  2:3079519/149‑1 (MQ=255)
                                                   cAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGAt                                                                                               >  1:3333751/1‑149 (MQ=255)
                                                    aGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATc                                                                                              <  2:3157158/149‑1 (MQ=255)
                                                     gTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATcc                                                                                             >  1:3957282/1‑149 (MQ=255)
                                                      tGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATccc                                                                                            <  1:3873749/149‑1 (MQ=255)
                                                         ttCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGt                                                                                         <  2:2587625/149‑1 (MQ=255)
                                                          tCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGtt                                                                                        <  2:3646935/149‑1 (MQ=255)
                                                          tCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACATCGATACCACGTTTGATCAGCAGCCAACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTACGGGGTAGCGACATCGCGCTCCCAGtt                                                                                        >  1:258609/1‑149 (MQ=255)
                                                            ggAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                                                      <  2:3944024/149‑1 (MQ=255)
                                                            ggAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                                                      <  2:4339798/149‑1 (MQ=255)
                                                               gTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGc                                                                                   >  2:386960/1‑149 (MQ=255)
                                                               gTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGc                                                                                   <  1:264999/149‑1 (MQ=255)
                                                                tACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGcc                                                                                  >  1:4542792/1‑149 (MQ=255)
                                                                    tGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAag                                                                              <  2:524871/149‑1 (MQ=255)
                                                                         cccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGAGCCCAGTTGATGCCGAAGTAgtg                                                                         <  2:3408328/149‑1 (MQ=255)
                                                                           cAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGtt                                                                       <  1:1948877/149‑1 (MQ=255)
                                                                            aGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGttt                                                                      <  1:1297692/149‑1 (MQ=255)
                                                                              cTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTAc                                                                    <  2:2307870/149‑1 (MQ=255)
                                                                                ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                                                                  >  2:1314207/1‑149 (MQ=255)
                                                                                ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                                                                  >  2:4160677/1‑149 (MQ=255)
                                                                                ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                                                                  >  2:2465559/1‑149 (MQ=255)
                                                                                 gAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAt                                                                 <  2:1821634/149‑1 (MQ=255)
                                                                                  aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATc                                                                <  1:3573955/149‑1 (MQ=255)
                                                                                  aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATc                                                                <  2:4456200/149‑1 (MQ=255)
                                                                                      tgtgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATg                                                            <  2:3711681/149‑1 (MQ=255)
                                                                                       gtgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGt                                                           >  2:673117/1‑149 (MQ=255)
                                                                                        tgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGtt                                                          <  2:1898619/149‑1 (MQ=255)
                                                                                         gACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTa                                                         >  2:3628048/1‑149 (MQ=255)
                                                                                          aCCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAg                                                        >  2:2608706/1‑149 (MQ=255)
                                                                                          aCCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAg                                                        >  1:3831077/1‑149 (MQ=255)
                                                                                          aCCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAg                                                        >  2:104245/1‑149 (MQ=255)
                                                                                           ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGc                                                       >  1:4722332/1‑149 (MQ=255)
                                                                                             aaCTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAc                                                     >  2:1589519/1‑149 (MQ=255)
                                                                                              aCTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAcc                                                    <  1:293601/149‑1 (MQ=255)
                                                                                              aCTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAcc                                                    <  2:973025/149‑1 (MQ=255)
                                                                                                  cGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACcagca                                                <  1:1089203/149‑1 (MQ=255)
                                                                                                    aTACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCagcagc                                              >  2:1429204/1‑149 (MQ=255)
                                                                                                    aTACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCagcagc                                              >  2:4205930/1‑149 (MQ=255)
                                                                                                     tACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCg                                             >  1:3493592/1‑149 (MQ=255)
                                                                                                      aCCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGa                                            >  1:2184853/1‑149 (MQ=255)
                                                                                                        cACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGaaa                                          >  1:527341/1‑149 (MQ=255)
                                                                                                        cACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGaaa                                          <  2:931437/149‑1 (MQ=255)
                                                                                                          cGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATc                                        >  1:3787923/1‑149 (MQ=255)
                                                                                                           gTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCa                                       <  2:2772364/149‑1 (MQ=255)
                                                                                                             ttGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACt                                     >  1:2712508/1‑149 (MQ=255)
                                                                                                               gATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCa                                   <  2:4660310/149‑1 (MQ=255)
                                                                                                                aTCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCAt                                  <  2:1174552/149‑1 (MQ=255)
                                                                                                                  cagcagCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcg                                >  1:1750593/1‑149 (MQ=255)
                                                                                                                    gcagcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgcc                              >  2:3367595/1‑149 (MQ=255)
                                                                                                                    gcagcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgcc                              >  1:2307793/1‑149 (MQ=255)
                                                                                                                    gcagcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgcc                              >  1:2361578/1‑149 (MQ=255)
                                                                                                                        ccTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCaa                          <  2:3815191/149‑1 (MQ=255)
                                                                                                                         cTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAac                         <  2:2855350/149‑1 (MQ=255)
                                                                                                                         cTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAac                         >  1:3111080/1‑149 (MQ=255)
                                                                                                                          tACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacg                        <  2:4502973/149‑1 (MQ=255)
                                                                                                                           aCCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacgg                       <  1:110833/149‑1 (MQ=255)
                                                                                                                            cccTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggt                      >  2:2171608/1‑149 (MQ=255)
                                                                                                                            cccTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggt                      <  2:3214116/149‑1 (MQ=255)
                                                                                                                              cTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtac                    >  1:1588513/1‑149 (MQ=255)
                                                                                                                                ggCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacgg                  >  1:2810760/1‑149 (MQ=255)
                                                                                                                                ggCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacgg                  >  2:3043225/1‑149 (MQ=255)
                                                                                                                                ggCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacgg                  <  2:2991291/149‑1 (MQ=255)
                                                                                                                                     tCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt             <  1:513891/149‑1 (MQ=255)
                                                                                                                                     tCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt             <  2:1975712/149‑1 (MQ=255)
                                                                                                                                     tCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt             <  2:4756795/149‑1 (MQ=255)
                                                                                                                                         ggCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcacc         <  2:2868594/149‑1 (MQ=255)
                                                                                                                                                aTCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGaa  >  1:4340742/1‑149 (MQ=255)
                                                                                                                                                aTCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGaa  >  1:2681351/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAA  >  CP009273/213937‑214229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑