Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,437,510 C→A 8.0% D47Y (GAC→TAC)  yfcL ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,437,5100CA 8.0% 1013.3 / 38.0 276D47Y (GAC→TAC) yfcLuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (125/128);  new base A (13/10);  total (138/138)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

GCCGCAACTTACTGCTGTGACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCT  >  CP009273/2437362‑2437658
                                                                                                                                                    |                                                                                                                                                    
gccgcAACTTACTGCTGTGACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGtc                                                                                                                                                      <  1:1104610/149‑1 (MQ=255)
   gcAACTTACTGCTGTGACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGtaatc                                                                                                                                                   <  2:1244588/149‑1 (MQ=255)
    cAACTTACTGCTGTGACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGtcatca                                                                                                                                                  <  2:384605/149‑1 (MQ=255)
      aCTTACTGCTGTGACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATcacc                                                                                                                                                >  2:1224681/1‑149 (MQ=255)
         tACTGCTGTGACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACt                                                                                                                                             <  2:2786465/149‑1 (MQ=255)
           ctgctgTGACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTAATCACCACttt                                                                                                                                           <  1:2773347/149‑1 (MQ=255)
            tgctgTGACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTc                                                                                                                                          <  2:4095815/149‑1 (MQ=255)
              ctgTGACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGGGTGGTCATCACCACTTTCCa                                                                                                                                        <  2:960144/149‑1 (MQ=255)
                   aCGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCt                                                                                                                                   <  1:1154109/149‑1 (MQ=255)
                       ctgctgAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGa                                                                                                                               >  1:927488/1‑149 (MQ=255)
                       ctgctgAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGa                                                                                                                               >  1:3707964/1‑149 (MQ=255)
                       ctgctgAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTAATCACCACTTTCCAGTTCTGCGa                                                                                                                               <  2:4390255/149‑1 (MQ=255)
                         gctgAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATg                                                                                                                             >  2:1152841/1‑149 (MQ=255)
                          ctgAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATgg                                                                                                                            <  1:1692430/149‑1 (MQ=255)
                          ctgAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATgg                                                                                                                            >  2:2645232/1‑149 (MQ=255)
                          ctgAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATgg                                                                                                                            <  2:1477858/149‑1 (MQ=255)
                           tgAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGc                                                                                                                           <  1:2794212/149‑1 (MQ=255)
                            gAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCt                                                                                                                          >  1:3103919/1‑149 (MQ=255)
                              aaCAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTaa                                                                                                                        >  2:3416244/1‑149 (MQ=255)
                              aaCAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTaa                                                                                                                        <  1:4311605/149‑1 (MQ=255)
                               aCAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTAATCACCACTTTCCAGTTCTGCGATGGCTAAt                                                                                                                       >  2:791247/1‑149 (MQ=255)
                                 aGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCACTGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATgc                                                                                                                     <  1:456762/149‑2 (MQ=255)
                                   gTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCa                                                                                                                   <  2:2392457/149‑1 (MQ=255)
                                   gTTTTCCCACATATCGGTCACCAGCACCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCa                                                                                                                   >  2:1395059/1‑149 (MQ=255)
                                     tttCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAgg                                                                                                                 >  2:3443627/1‑149 (MQ=255)
                                       tCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTg                                                                                                               <  1:3215093/149‑1 (MQ=255)
                                         ccACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGc                                                                                                             <  2:599493/149‑1 (MQ=255)
                                          cacaTATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGcc                                                                                                            <  1:1095778/149‑1 (MQ=255)
                                          cacaTATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGcc                                                                                                            >  1:2897581/1‑149 (MQ=255)
                                          cacaTATCGGTCAACAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGATTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGACATCACCACTTACAAGTTCTGAGATGGCTAATGTCAGGTGTcc                                                                                                            >  2:3710337/1‑149 (MQ=255)
                                                   gTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATa                                                                                                   >  1:3150314/1‑149 (MQ=255)
                                                      aCCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATAccc                                                                                                >  1:828358/1‑149 (MQ=255)
                                                      aCCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATAccc                                                                                                >  2:2667971/1‑149 (MQ=255)
                                                        cAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCAc                                                                                              >  1:146847/1‑149 (MQ=255)
                                                        cAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCAc                                                                                              >  2:1935104/1‑149 (MQ=255)
                                                          gcgcCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTg                                                                                            >  2:3650433/1‑149 (MQ=255)
                                                            gcCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGc                                                                                          <  2:197041/149‑1 (MQ=255)
                                                             ccTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCa                                                                                         <  1:2165173/149‑1 (MQ=255)
                                                                 gTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTAATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAaaca                                                                                     >  1:4164908/1‑149 (MQ=255)
                                                                  tCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAaacaa                                                                                    >  2:3982762/1‑149 (MQ=255)
                                                                    aCGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACt                                                                                  <  1:3443453/149‑1 (MQ=255)
                                                                       cggcgACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACtcat                                                                               <  2:4593288/149‑1 (MQ=255)
                                                                         gcgACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACtcatca                                                                             >  1:2071624/1‑149 (MQ=255)
                                                                          cgACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACtcatcat                                                                            <  2:2630042/149‑1 (MQ=255)
                                                                          cgACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACtcatcat                                                                            >  1:3986286/1‑149 (MQ=255)
                                                                            aCAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACtcatcatca                                                                          >  1:539339/1‑149 (MQ=255)
                                                                             cAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCAc                                                                         <  2:268615/149‑1 (MQ=255)
                                                                             cAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCAc                                                                         <  2:3748069/149‑1 (MQ=255)
                                                                              aaTTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACt                                                                        >  1:1957014/1‑149 (MQ=255)
                                                                              aaTTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACt                                                                        >  2:4395194/1‑149 (MQ=255)
                                                                              aaTTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTCTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACt                                                                        <  1:271085/149‑1 (MQ=255)
                                                                               aTTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTg                                                                       >  1:1273533/1‑149 (MQ=255)
                                                                                    ccAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATg                                                                  <  2:2507913/149‑1 (MQ=255)
                                                                                    ccAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATg                                                                  <  1:842359/149‑1 (MQ=255)
                                                                                    ccAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATg                                                                  >  1:970277/1‑149 (MQ=255)
                                                                                    ccAGCACCAATGGCTTTTTCCAGACTCGGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCTAATACCCACTGGCAAACAACTCATCATCACTGGCATg                                                                  <  2:3277191/149‑1 (MQ=255)
                                                                                     cAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATgg                                                                 <  2:695496/149‑1 (MQ=255)
                                                                                       gCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTc                                                               >  2:347337/1‑149 (MQ=255)
                                                                                        cACCAATGGCTTGTTCCAGACTCTGGCTAACGGTCGTCTGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAAGACCCACTGGCAAACAACTCATCATCACTGGCATGGTCa                                                              <  2:4537215/149‑1 (MQ=255)
                                                                                         aCCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCaa                                                             <  1:8879/149‑1 (MQ=255)
                                                                                         aCCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCaa                                                             <  2:564687/149‑1 (MQ=255)
                                                                                          ccAATGGCCTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAAc                                                            >  1:3008059/1‑149 (MQ=255)
                                                                                           cAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAAcc                                                           >  1:1370592/1‑149 (MQ=255)
                                                                                            aaTGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCa                                                          <  2:1996588/149‑1 (MQ=255)
                                                                                               ggCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATAc                                                       <  1:4750329/149‑1 (MQ=255)
                                                                                                gCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATAcc                                                      >  1:1034272/1‑149 (MQ=255)
                                                                                                 cTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCg                                                     <  1:696644/149‑1 (MQ=255)
                                                                                                  tttttCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGt                                                    <  2:171759/149‑1 (MQ=255)
                                                                                                     ttCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGa                                                 <  1:4344764/149‑1 (MQ=255)
                                                                                                     ttCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGa                                                 <  2:2874051/149‑1 (MQ=255)
                                                                                                      tCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGAt                                                <  2:3889490/149‑1 (MQ=255)
                                                                                                        cAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTAATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTa                                              >  1:3488959/1‑149 (MQ=255)
                                                                                                         aGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTaa                                             <  1:3593881/149‑1 (MQ=255)
                                                                                                         aGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTaa                                             >  2:4243419/1‑149 (MQ=255)
                                                                                                          gACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAAt                                            >  2:3192457/1‑149 (MQ=255)
                                                                                                             tctGTCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGTTGGCGATTGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCTCTGGCTTGGTCAAACATACCGTCGATTTATGcc                                         <  2:2483407/149‑1 (MQ=255)
                                                                                                                 gCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGaa                                     >  1:3056872/1‑149 (MQ=255)
                                                                                                                 gCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGaa                                     <  1:4664266/149‑1 (MQ=255)
                                                                                                                    aaCGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGc                                  <  2:1865510/149‑1 (MQ=255)
                                                                                                                       ggTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTg                               <  2:2728031/149‑1 (MQ=255)
                                                                                                                       ggTCGTATGCACCGCCTGAGCGGAGTGGTAATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATTCCAGAATGCGTg                               >  2:4563565/1‑149 (MQ=255)
                                                                                                                         tCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGAt                             <  1:4084485/149‑1 (MQ=255)
                                                                                                                         tCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTACTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGAt                             <  1:1999060/149‑1 (MQ=255)
                                                                                                                         gcGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTGCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACGGGCAAACAACTCATCATCCCTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGAt                             <  1:1630378/148‑1 (MQ=255)
                                                                                                                           gTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTc                           >  1:9186/1‑149 (MQ=255)
                                                                                                                            tATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCa                          >  1:580863/1‑149 (MQ=255)
                                                                                                                             aTGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCaa                         >  1:469907/1‑149 (MQ=255)
                                                                                                                              tGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCaaa                        <  1:1725406/149‑1 (MQ=255)
                                                                                                                                cACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACt                      <  1:1516140/149‑1 (MQ=255)
                                                                                                                                  ccGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTcc                    <  2:3103110/149‑1 (MQ=255)
                                                                                                                                     ccTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATTCGTGATTCAAACTCcgcg                 >  1:29572/1‑149 (MQ=255)
                                                                                                                                     ccTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCcgcg                 >  1:4185504/1‑149 (MQ=255)
                                                                                                                                         aGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGatca             <  1:1390656/149‑1 (MQ=255)
                                                                                                                                         aGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGatca             <  1:241779/149‑1 (MQ=255)
                                                                                                                                             gAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCAtctt         >  2:2596717/1‑149 (MQ=255)
                                                                                                                                             gAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCAtctt         >  1:3073119/1‑149 (MQ=255)
                                                                                                                                             gAGTGGTAATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCAtctt         >  2:2989082/1‑149 (MQ=255)
                                                                                                                                              aGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCAtcttc        <  2:2897441/149‑1 (MQ=255)
                                                                                                                                                 ggTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCttt     <  1:3821785/149‑1 (MQ=255)
                                                                                                                                                  gTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTc    <  1:4123344/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCGCAACTTACTGCTGTGACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCT  >  CP009273/2437362‑2437658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑