BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,456,705	C→A	86.5%	S72Y (TCC→TAC)	yfdF →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,456,705	0	C	A	86.5%	466.8 / 75.2	193	S72Y (TCC→TAC)	yfdF	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (13/13); new base A (96/71); total (109/84)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 193 total aligned reads displayed.

TGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAGAC  >  CP009273/2456559‑2456852

tgatgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCt                                                                                                                                                   <  2:1958130/149‑1 (MQ=255)

tgatgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACt                                                                                                                                                   >  2:2022857/1‑149 (MQ=255)

tgatgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACt                                                                                                                                                   <  1:3051500/149‑1 (MQ=255)

  atgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCa                                                                                                                                                 <  2:2348877/149‑1 (MQ=255)

  atgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCa                                                                                                                                                 >  1:835139/1‑149 (MQ=255)

  atgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCa                                                                                                                                                 <  2:4745423/149‑1 (MQ=255)

  atgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCa                                                                                                                                                 >  1:2528340/1‑149 (MQ=255)

     aaGTTAATGGATTAGTACAAGAGTTCAAACACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACTTCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATGACTCAGtt                                                                                                                                              <  1:714938/149‑1 (MQ=255)

     aaGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGtt                                                                                                                                              >  1:2303961/1‑149 (MQ=255)

     aaGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGtt                                                                                                                                              >  1:4413717/1‑149 (MQ=255)

        ttAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACt                                                                                                                                           <  2:2896299/149‑1 (MQ=255)

          aaTGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTta                                                                                                                                         <  2:2379820/149‑1 (MQ=255)

          aaTGGATTAGTACAAGAGTTCAAAAACCTTTATAATGGTAAGGAAGGAACAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTta                                                                                                                                         <  2:2379694/149‑1 (MQ=255)

           aTGGATTAGTACAAGATTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTtat                                                                                                                                        <  2:2824067/149‑1 (MQ=255)

           aTGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTtat                                                                                                                                        <  2:4083173/149‑1 (MQ=255)

           aTGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTtat                                                                                                                                        >  1:2469824/1‑149 (MQ=255)

            tGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTtata                                                                                                                                       >  1:2027097/1‑149 (MQ=255)

              gATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatatt                                                                                                                                     >  2:3558248/1‑149 (MQ=255)

                ttAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattga                                                                                                                                   >  2:264291/1‑149 (MQ=255)

                  aGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgata                                                                                                                                 >  1:2117810/1‑149 (MQ=255)

                  aGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgata                                                                                                                                 >  1:4589916/1‑149 (MQ=255)

                   gTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgatat                                                                                                                                >  2:8762/1‑149 (MQ=255)

                    tACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgatatt                                                                                                                               >  2:725494/1‑149 (MQ=255)

                       aaGATTACAAAAACAATTTTAATGGTAAGGAAGCAATAACCACCTTTATTAAACATATACTTGAGCTTAAAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGAc                                                                                                                            <  1:1476181/149‑1 (MQ=255)

                       aaGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGAc                                                                                                                            >  1:3500227/1‑149 (MQ=255)

                         gagTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTc                                                                                                                          >  2:263054/1‑149 (MQ=255)

                            ttCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCaa                                                                                                                       >  2:3234452/1‑149 (MQ=255)

                             tCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAAt                                                                                                                      <  1:2941243/149‑1 (MQ=255)

                              cAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATg                                                                                                                     >  1:1808985/1‑149 (MQ=255)

                              cAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATATAATATTAATAATTACTCAGTTACTTATATTTATATTGACTCCAATg                                                                                                                     >  1:4198172/1‑149 (MQ=255)

                               aaaaaCCTTTTTATTGGTAAGGAAGGAATAAGCAACTGTATTAAACATCTACTTTAGATTATAAAAAACGCCATAAGAGTAAACGTCGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGa                                                                                                                    <  2:4348690/149‑1 (MQ=255)

                               aaaaaCCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGa                                                                                                                    >  2:1266353/1‑149 (MQ=255)

                               aaaaaCCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGa                                                                                                                    <  2:823506/149‑1 (MQ=255)

                                   aCCTTTTTAATGTTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACa                                                                                                                <  1:4170177/149‑1 (MQ=255)

                                             tGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTa                                                                                                      >  1:3589234/1‑149 (MQ=255)

                                              ggTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTAc                                                                                                     <  2:1950829/149‑1 (MQ=255)

                                               gTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACt                                                                                                    >  2:4230954/1‑149 (MQ=255)

                                                tAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTa                                                                                                   >  1:2358344/1‑149 (MQ=255)

                                                 aaggaaggAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTAt                                                                                                  >  1:4330221/1‑149 (MQ=255)

                                                 aaggaaggAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTAt                                                                                                  >  1:4743527/1‑149 (MQ=255)

                                                     aaggaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGt                                                                                              >  1:2947033/1‑149 (MQ=255)

                                                          aTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACCCCATACGAGTAAAAGTCGATCCATAAAAATTTAATAATAATAATTACTCCGGTACGTATATTGTTATTGAAAACAAATATAAAGTCCAAAATTCTTATGGTATCGa                                                                                         >  2:3597054/1‑149 (MQ=255)

                                                           tAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGAc                                                                                        >  1:4316739/1‑149 (MQ=255)

                                                              gCACCTGTATTAAACATCTACTTGATCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAAc                                                                                     <  2:3761594/149‑1 (MQ=255)

                                                               cACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAAcc                                                                                    >  1:916023/1‑149 (MQ=255)

                                                               cACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAAcc                                                                                    <  1:1143330/149‑1 (MQ=255)

                                                                aCCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCa                                                                                   >  1:3547190/1‑149 (MQ=255)

                                                                 ccTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCaa                                                                                  >  2:1212431/1‑149 (MQ=255)

                                                                      aTTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAAcc                                                                             <  2:3170580/149‑1 (MQ=255)

                                                                         aaaCATCTACTTGATCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAAt                                                                          <  2:4649395/149‑1 (MQ=255)

                                                                          aaCATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATa                                                                         >  1:78396/1‑149 (MQ=255)

                                                                             aTCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGaa                                                                      >  2:1090884/1‑149 (MQ=255)

                                                                              tCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAAt                                                                     >  1:897660/1‑149 (MQ=255)

                                                                              tCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAAt                                                                     >  2:1675406/1‑149 (MQ=255)

                                                                                      aGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCg                                                             >  1:958856/1‑149 (MQ=255)

                                                                                          tataAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACt                                                         <  2:3489539/149‑1 (MQ=255)

                                                                                           ataAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACta                                                        <  1:1437588/149‑1 (MQ=255)

                                                                                            taAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACtat                                                       >  2:1488400/1‑149 (MQ=255)

                                                                                             aaaaaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACtata                                                      >  2:3958296/1‑149 (MQ=255)

                                                                                             aaaaaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACtata                                                      >  2:52258/1‑149 (MQ=255)

                                                                                              aaaaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATaa                                                     <  1:4175725/149‑1 (MQ=255)

                                                                                                aaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAg                                                   >  1:54689/1‑149 (MQ=255)

                                                                                                 aaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCACTAGAATTACCTGCGAACTATAAAGa                                                  <  2:4310762/149‑1 (MQ=255)

                                                                                                 aaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGa                                                  <  2:4310403/149‑1 (MQ=255)

                                                                                                     ccATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACaaa                                              >  1:2545876/1‑149 (MQ=255)

                                                                                                     ccATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACaaa                                              <  2:612968/149‑1 (MQ=255)

                                                                                                      cATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAg                                             >  1:4304255/1‑149 (MQ=255)

                                                                                                        tACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGaa                                           <  1:1781516/149‑1 (MQ=255)

                                                                                                           gAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTc                                        <  1:3793456/149‑1 (MQ=255)

                                                                                                            aGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCg                                       <  2:1843927/149‑1 (MQ=255)

                                                                                                            aGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCg                                       <  2:1843366/149‑1 (MQ=255)

                                                                                                             gTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGt                                      >  2:3850061/1‑149 (MQ=255)

                                                                                                              tAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTc                                     <  2:3027669/149‑1 (MQ=255)

                                                                                                               aaaCGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTcc                                    >  1:3303321/1‑149 (MQ=255)

                                                                                                               aaaCGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTcc                                    <  2:2972997/149‑1 (MQ=255)

                                                                                                                   gacgaTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTAc                                >  1:2796857/1‑149 (MQ=255)

                                                                                                                       aTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATc                            >  1:1170649/1‑149 (MQ=255)

                                                                                                                       aTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATc                            >  1:4002060/1‑149 (MQ=255)

                                                                                                                       aTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATc                            >  1:657714/1‑149 (MQ=255)

                                                                                                                        tCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCa                           <  1:842780/149‑1 (MQ=255)

                                                                                                                        tCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCa                           <  1:842800/149‑1 (MQ=255)

                                                                                                                         ccTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCAt                          >  2:1662190/1‑149 (MQ=255)

                                                                                                                          cTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCAtt                         <  2:1233918/149‑1 (MQ=255)

                                                                                                                           ttATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTa                        >  1:129668/1‑149 (MQ=255)

                                                                                                                            tataGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTaa                       <  1:1362807/149‑1 (MQ=255)

                                                                                                                              taGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATg                     >  2:1318484/1‑149 (MQ=255)

                                                                                                                                     aatattaataATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACAt              <  1:3400414/149‑1 (MQ=255)

                                                                                                                                     aatattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACAt              <  2:2153735/149‑1 (MQ=255)

                                                                                                                                     aatattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACAt              >  2:3638942/1‑149 (MQ=255)

                                                                                                                                     aatattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACAt              >  2:3638860/1‑149 (MQ=255)

                                                                                                                                     aatattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACAt              <  2:4518022/149‑1 (MQ=255)

                                                                                                                                      atattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATa             >  1:4519323/1‑149 (MQ=255)

                                                                                                                                          taataaTTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTg         >  2:1380994/1‑149 (MQ=255)

                                                                                                                                           aataatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTga        <  2:2487907/149‑1 (MQ=255)

                                                                                                                                           aataatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTga        >  2:272289/1‑149 (MQ=255)

                                                                                                                                           aataatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTga        >  2:272370/1‑149 (MQ=255)

                                                                                                                                              aatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGagaa     <  1:3377964/149‑1 (MQ=255)

                                                                                                                                              aatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGagaa     >  1:3095840/1‑149 (MQ=255)

                                                                                                                                                ttCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGagaaga   >  2:454908/1‑149 (MQ=255)

                                                                                                                                                 tCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAGAc  >  2:2572803/1‑149 (MQ=255)

TGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAGAC  >  CP009273/2456559‑2456852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`