Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,549,676 C→A 100% L28L (CTG→CTT)  eutL ← putative ethanol utilization carboxysome structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,549,6760CA99.7% 1047.0 / ‑4.4 304L28L (CTG→CTT) eutLputative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (154/149);  total (154/150)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 304 total aligned reads displayed.

GGGCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTCAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGCCA  >  CP009273/2549530‑2549821
                                                                                                                                                  |                                                                                                                                                 
gggCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAg                                                                                                                                                 >  2:2415227/1‑149 (MQ=255)
   ccGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTc                                                                                                                                              <  1:2859074/149‑1 (MQ=255)
    cGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCa                                                                                                                                             <  2:3948797/149‑1 (MQ=255)
    cGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCa                                                                                                                                             <  2:3792348/149‑1 (MQ=255)
    cGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCa                                                                                                                                             <  2:1558436/149‑1 (MQ=255)
    cGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCa                                                                                                                                             <  1:3987825/149‑1 (MQ=255)
     gtgtGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCAc                                                                                                                                            <  1:3290311/149‑1 (MQ=255)
       gtgCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCAcgc                                                                                                                                          <  1:3913545/149‑1 (MQ=255)
         gCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACgcgc                                                                                                                                        >  2:2063739/1‑149 (MQ=255)
              gcCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAAt                                                                                                                                   <  2:1650973/149‑1 (MQ=255)
              gcCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAAt                                                                                                                                   <  2:1252749/149‑1 (MQ=255)
               cccGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATc                                                                                                                                  >  2:550621/1‑149 (MQ=255)
               cccGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATc                                                                                                                                  >  2:2941662/1‑149 (MQ=255)
                   cATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCg                                                                                                                              >  2:2173242/1‑149 (MQ=255)
                    atatAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGt                                                                                                                             <  1:1097239/149‑1 (MQ=255)
                    atatAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGt                                                                                                                             <  2:1890780/149‑1 (MQ=255)
                     tataGCGAGCGGCCATACACGACTTCCAGCATCGCCTGATTGGTCGATTCATCAGGGGCAATGTAGGTGACGTCATCGTAGTCGGCAGTAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTGACGCGCAAAATCGGCGtt                                                                                                                            <  1:3327165/149‑1 (MQ=255)
                     tataGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGtt                                                                                                                            <  1:1712265/149‑1 (MQ=255)
                     tataGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGtt                                                                                                                            <  2:4019428/149‑1 (MQ=255)
                       taGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTaa                                                                                                                          >  2:2719703/1‑149 (MQ=255)
                       taGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCACAAAATCGGCGTTaa                                                                                                                          >  1:2557616/1‑149 (MQ=255)
                        agcgagcgGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAAc                                                                                                                         >  2:297562/1‑149 (MQ=255)
                                gCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCaa                                                                                                                 <  2:1651797/149‑1 (MQ=255)
                                gCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCaa                                                                                                                 >  1:4707652/1‑149 (MQ=255)
                                  cATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATc                                                                                                               <  1:1576232/149‑1 (MQ=255)
                                     acacCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCAcc                                                                                                            <  2:1689514/149‑1 (MQ=255)
                                     acacCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCAcc                                                                                                            >  2:355076/1‑149 (MQ=255)
                                      caccacTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCAccc                                                                                                           <  1:570314/149‑1 (MQ=255)
                                       accacTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCg                                                                                                          >  2:1596864/1‑149 (MQ=255)
                                       accacTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCg                                                                                                          >  1:854090/1‑149 (MQ=255)
                                          acTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCAt                                                                                                       >  1:21404/1‑149 (MQ=255)
                                             tCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGc                                                                                                    <  1:3932296/149‑1 (MQ=255)
                                              ccaccaTCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATAGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAAGTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCg                                                                                                   >  1:4473600/1‑149 (MQ=255)
                                                  caTCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGa                                                                                               >  1:2436248/1‑149 (MQ=255)
                                                  caTCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGa                                                                                               >  1:4318611/1‑149 (MQ=255)
                                                      gCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGa                                                                                           <  2:1784898/149‑1 (MQ=255)
                                                       ccTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGAc                                                                                          <  2:4677791/149‑1 (MQ=255)
                                                          gCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGt                                                                                       <  2:4601128/149‑1 (MQ=255)
                                                          gCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGt                                                                                       <  2:4390887/149‑1 (MQ=255)
                                                              ggTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGaa                                                                                   <  2:2353265/149‑1 (MQ=255)
                                                                tCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATc                                                                                 <  1:2935638/149‑1 (MQ=255)
                                                                  gCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCaa                                                                               >  2:3875870/1‑149 (MQ=255)
                                                                   cTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCaaa                                                                              >  1:2276567/1‑149 (MQ=255)
                                                                     tcatcaGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATc                                                                            <  1:2918592/149‑1 (MQ=255)
                                                                       atcaGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTa                                                                          >  1:915125/1‑149 (MQ=255)
                                                                        tcaGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTaa                                                                         >  2:4562972/1‑149 (MQ=255)
                                                                        tcaGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTaa                                                                         >  1:771217/1‑149 (MQ=255)
                                                                         caGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTaaa                                                                        <  1:3521638/149‑1 (MQ=255)
                                                                         caGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTaaa                                                                        <  1:2567454/149‑1 (MQ=255)
                                                                          aGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAg                                                                       >  2:1761740/1‑149 (MQ=255)
                                                                            cGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCt                                                                     <  1:1454352/149‑1 (MQ=255)
                                                                             ggCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTg                                                                    >  2:3613724/1‑149 (MQ=255)
                                                                                aaTGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCa                                                                 >  1:607034/1‑149 (MQ=255)
                                                                                aaTGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCa                                                                 >  1:1387257/1‑149 (MQ=255)
                                                                                 aTGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCat                                                                >  2:1707411/1‑149 (MQ=255)
                                                                                  tGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCatg                                                               <  2:4451730/149‑1 (MQ=255)
                                                                                   gTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCatga                                                              <  2:2495382/149‑1 (MQ=255)
                                                                                      ggTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGc                                                           >  2:3533467/1‑149 (MQ=255)
                                                                                      ggTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGc                                                           >  2:228486/1‑149 (MQ=255)
                                                                                       gTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGcc                                                          >  1:4602894/1‑149 (MQ=255)
                                                                                          aCGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGcctcc                                                       <  2:4095228/149‑1 (MQ=255)
                                                                                                tCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCg                                                 >  2:730194/1‑149 (MQ=255)
                                                                                                tCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCg                                                 >  2:1297132/1‑149 (MQ=255)
                                                                                                 cGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCgg                                                >  1:4482322/1‑149 (MQ=255)
                                                                                                   gAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGt                                              <  2:922145/149‑1 (MQ=255)
                                                                                                   gAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGt                                              >  1:3071469/1‑149 (MQ=255)
                                                                                                     gTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCACTCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCa                                            >  1:2736438/1‑149 (MQ=255)
                                                                                                     gTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCa                                            >  1:1946404/1‑149 (MQ=255)
                                                                                                        ggCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTCAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGt                                         <  2:4295945/149‑1 (MQ=255)
                                                                                                        ggCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGt                                         <  2:257056/149‑1 (MQ=255)
                                                                                                            gAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGAt                                     <  2:4490367/149‑1 (MQ=255)
                                                                                                            gAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGAt                                     <  1:3410881/149‑1 (MQ=255)
                                                                                                             aaaTCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATg                                    >  2:4147809/1‑149 (MQ=255)
                                                                                                                 cAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCgg                                <  1:3626360/149‑1 (MQ=255)
                                                                                                                  aGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGa                               >  1:2043650/1‑149 (MQ=255)
                                                                                                                   gACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGAt                              <  1:3275503/149‑1 (MQ=255)
                                                                                                                   gACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGAt                              <  1:19233/149‑1 (MQ=255)
                                                                                                                   gACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGAt                              <  1:1003524/149‑1 (MQ=255)
                                                                                                                   gACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGAt                              <  1:3089278/149‑1 (MQ=255)
                                                                                                                       gAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAATATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCtt                          <  1:3564282/149‑1 (MQ=255)
                                                                                                                        aGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCttt                         >  2:3294840/1‑149 (MQ=255)
                                                                                                                         ggCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTc                        >  2:1063035/1‑149 (MQ=255)
                                                                                                                           cTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTg                      >  2:3378222/1‑149 (MQ=255)
                                                                                                                           cTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTg                      >  1:4267920/1‑149 (MQ=255)
                                                                                                                            tACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGc                     >  2:1461115/1‑149 (MQ=255)
                                                                                                                            tACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGc                     <  2:660850/149‑1 (MQ=255)
                                                                                                                                aaTATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCa                 >  1:2083229/1‑149 (MQ=255)
                                                                                                                                 atatGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAg                >  2:4224507/1‑149 (MQ=255)
                                                                                                                                 atatGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAg                >  2:4725151/1‑149 (MQ=255)
                                                                                                                                 atatGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAg                >  2:2814027/1‑149 (MQ=255)
                                                                                                                                    tGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCAt             >  1:3696391/1‑149 (MQ=255)
                                                                                                                                     gcggcgGCAATTTAAGTTCACGCTCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATa            <  1:4223297/149‑1 (MQ=255)
                                                                                                                                     gcggcgGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATa            >  1:1777630/1‑149 (MQ=255)
                                                                                                                                     gcggcgGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATa            <  1:4366088/149‑1 (MQ=255)
                                                                                                                                        gcggcAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGt         >  1:3099828/1‑149 (MQ=255)
                                                                                                                                        gcggcAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGt         >  1:1275045/1‑149 (MQ=255)
                                                                                                                                          ggcAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGttt       <  1:1794225/149‑1 (MQ=255)
                                                                                                                                            cAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCg     >  2:1819510/1‑149 (MQ=255)
                                                                                                                                              aTTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGcc   >  2:2926690/1‑149 (MQ=255)
                                                                                                                                               tttAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGCCa  >  2:2206887/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGGCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTCAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGCCA  >  CP009273/2549530‑2549821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑