Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,606,516 G→T 100% D420Y (GAC→TAC)  hyfR → hydrogenase‑4 transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,606,5160GT100.0% 1012.5 / NA 298D420Y (GAC→TAC) hyfRhydrogenase‑4 transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (123/175);  total (123/175)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 299 total aligned reads displayed.

GGTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGAT  >  CP009273/2606368‑2606661
                                                                                                                                                    |                                                                                                                                                 
     tGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACaaa                                                                                                                                              <  2:2390605/149‑1 (MQ=255)
     tGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACaaa                                                                                                                                              <  2:1701190/149‑1 (MQ=255)
        tttGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAggg                                                                                                                                           >  1:671410/1‑149 (MQ=255)
        tttGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAggg                                                                                                                                           >  1:2476152/1‑149 (MQ=255)
         ttGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAgggg                                                                                                                                          <  1:1263291/149‑1 (MQ=255)
           gtggtgACACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCg                                                                                                                                        <  2:1905351/149‑1 (MQ=255)
              gtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGttt                                                                                                                                     <  2:1525368/149‑1 (MQ=255)
              gtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCGGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGttt                                                                                                                                     <  2:134436/149‑1 (MQ=255)
               tgACACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTa                                                                                                                                    <  2:3721146/149‑1 (MQ=255)
                gAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTAc                                                                                                                                   <  2:1271387/149‑1 (MQ=255)
                 aaaCCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACt                                                                                                                                  <  2:1154675/149‑1 (MQ=255)
                    ccGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGt                                                                                                                               <  2:458615/149‑1 (MQ=255)
                     cGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTg                                                                                                                              >  1:1656779/1‑149 (MQ=255)
                     cGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTg                                                                                                                              >  2:104629/1‑149 (MQ=255)
                     cGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTg                                                                                                                              <  1:4028755/149‑1 (MQ=255)
                         aCTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGAt                                                                                                                          <  1:840584/149‑1 (MQ=255)
                          cTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGAtt                                                                                                                         <  1:2872760/149‑1 (MQ=255)
                            ggCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTaa                                                                                                                       >  2:3803152/1‑149 (MQ=255)
                             gCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAAt                                                                                                                      >  1:803427/1‑149 (MQ=255)
                              cAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATa                                                                                                                     <  2:331990/149‑1 (MQ=255)
                               aaaGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCGGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAc                                                                                                                    >  2:4749518/1‑149 (MQ=255)
                                aaGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAcc                                                                                                                   <  2:4069077/149‑1 (MQ=255)
                                 agagGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAccc                                                                                                                  >  2:2435897/1‑149 (MQ=255)
                                   agGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCAt                                                                                                                <  1:608837/149‑1 (MQ=255)
                                   agGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCAt                                                                                                                <  1:1010033/149‑1 (MQ=255)
                                      tGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGt                                                                                                             <  2:306291/149‑1 (MQ=255)
                                       gATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTg                                                                                                            <  2:3204722/149‑1 (MQ=255)
                                        aTCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTgg                                                                                                           <  1:2585520/149‑1 (MQ=255)
                                        aTCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTgg                                                                                                           <  1:2066128/149‑1 (MQ=255)
                                         tCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGt                                                                                                          <  1:3177237/149‑1 (MQ=255)
                                         tCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGt                                                                                                          <  1:4247600/149‑1 (MQ=255)
                                              agagCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGttt                                                                                                     >  2:4534648/1‑149 (MQ=255)
                                              agagCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGttt                                                                                                     <  1:2246369/149‑1 (MQ=255)
                                              agagCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGttt                                                                                                     <  1:3098397/149‑1 (MQ=255)
                                               gagCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGtttt                                                                                                    >  2:342569/1‑149 (MQ=255)
                                               gagCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGtttt                                                                                                    <  2:4015783/149‑1 (MQ=255)
                                                   gATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGaaa                                                                                                >  1:3950406/1‑149 (MQ=255)
                                                    aTCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAt                                                                                               >  1:4095668/1‑149 (MQ=255)
                                                    aTCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAt                                                                                               <  1:3046904/149‑1 (MQ=255)
                                                     tCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAtt                                                                                              <  1:4079423/149‑1 (MQ=255)
                                                           aaCTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGAt                                                                                        >  2:4431816/1‑149 (MQ=255)
                                                           aaCTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGAt                                                                                        <  2:4141224/149‑1 (MQ=255)
                                                           aaCTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGAt                                                                                        <  2:1428602/149‑1 (MQ=255)
                                                            aCTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATg                                                                                       <  2:890913/149‑1 (MQ=255)
                                                                aGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATggcgg                                                                                   >  2:3868180/1‑149 (MQ=255)
                                                                 gCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCGGGAAAGTGAGTTAGTCGGTCATTACGATGGGGCGGTTAGTGGTGCGCTTAATAGCCCTCGTGGTGGTGTGGCTATTGCCGAGggcggc                                                                                  >  2:2594101/1‑149 (MQ=255)
                                                                       gacgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACgttg                                                                            >  2:553089/1‑149 (MQ=255)
                                                                       gacgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACgttg                                                                            >  2:1457033/1‑149 (MQ=255)
                                                                        acgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACgttgt                                                                           >  1:157958/1‑149 (MQ=255)
                                                                        acgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACgttgt                                                                           >  1:2316741/1‑149 (MQ=255)
                                                                         cgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACgttgtt                                                                          <  1:3893512/149‑1 (MQ=255)
                                                                         cgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACgttgtt                                                                          <  2:3539535/149‑1 (MQ=255)
                                                                          gcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGttt                                                                         <  1:2747047/149‑1 (MQ=255)
                                                                           cgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAATGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTtc                                                                        <  2:4379575/149‑1 (MQ=255)
                                                                            gacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTtct                                                                       <  2:1656779/149‑1 (MQ=255)
                                                                             acAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTtctc                                                                      <  2:506037/149‑1 (MQ=255)
                                                                                    gCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGaaa                                                               <  1:677184/149‑1 (MQ=255)
                                                                                     cTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAAt                                                              <  2:1226193/149‑1 (MQ=255)
                                                                                     cTGGTCAAAATCAACTGCGCGGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAAt                                                              <  2:3616307/149‑1 (MQ=255)
                                                                                      tGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAAtt                                                             <  1:3193321/149‑1 (MQ=255)
                                                                                      tGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAAtt                                                             <  2:1362162/149‑1 (MQ=255)
                                                                                         tCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGTTGAAATTGGc                                                          >  1:531905/1‑149 (MQ=255)
                                                                                         tCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGc                                                          <  1:2105456/149‑1 (MQ=255)
                                                                                         tCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGGTGTTTCTCGATGAAATTGGc                                                          >  1:1030829/1‑149 (MQ=255)
                                                                                           aaaaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTATCGATGAAATTTGCGa                                                        >  1:4067596/1‑149 (MQ=255)
                                                                                           aaaaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGa                                                        <  1:3234759/149‑1 (MQ=255)
                                                                                            aaaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGAt                                                       <  1:4747867/149‑1 (MQ=255)
                                                                                            aaaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGAt                                                       <  2:3107575/149‑1 (MQ=255)
                                                                                             actCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATc                                                      <  1:3293656/147‑1 (MQ=255)
                                                                                             aaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATc                                                      <  1:1979293/149‑1 (MQ=255)
                                                                                             aaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATc                                                      <  1:2928842/149‑1 (MQ=255)
                                                                                                  aCTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCg                                                 <  1:3028872/149‑1 (MQ=255)
                                                                                                    tGCGCTGCCATCCCCGCCAGTCGTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAGTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGtt                                               <  2:4571997/149‑1 (MQ=255)
                                                                                                       gcTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGa                                            >  2:2739353/1‑149 (MQ=255)
                                                                                                         tGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAAc                                          <  1:2614931/149‑1 (MQ=255)
                                                                                                         tGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAAc                                          <  1:13544/149‑1 (MQ=255)
                                                                                                             aTCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCa                                      >  2:2507775/1‑149 (MQ=255)
                                                                                                                cccGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGcc                                   >  2:2980012/1‑149 (MQ=255)
                                                                                                                cccGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGcc                                   >  2:4301991/1‑149 (MQ=255)
                                                                                                                cccGCCAGTCTTCTGGAAAGTGAGTTAGTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGcc                                   <  1:2401076/149‑1 (MQ=255)
                                                                                                                 ccGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGTAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGGTAGAACTTCAGCCt                                  >  1:3341353/1‑149 (MQ=255)
                                                                                                                     cAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGTAATTGACGATTGCGGCACGTTGTTTATAGATGAAAATTGCGATCTTCCGTTATAACTTCATCCaaaaa                              >  1:1224524/1‑148 (MQ=255)
                                                                                                                     cAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAAc                              <  2:4295485/149‑1 (MQ=255)
                                                                                                                      aGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCAAAAct                             >  1:3399045/1‑149 (MQ=255)
                                                                                                                       gTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActg                            <  1:4510131/149‑1 (MQ=255)
                                                                                                                        tcttctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActgc                           <  1:928953/149‑1 (MQ=255)
                                                                                                                         cttctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTAAGCCTAAAatgct                          >  1:3767890/1‑149 (MQ=255)
                                                                                                                          ttctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActgctg                         <  2:696841/149‑1 (MQ=255)
                                                                                                                               gAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTTGCGATCTGCCGTTAGAACTTCATCCTAAACTGCTTCGCGt                    >  1:4331853/1‑149 (MQ=255)
                                                                                                                                aaaGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGTAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATATGCCGTTAGAACTTCAGCCTAAAATGCTGCGCGTa                   >  1:4440435/1‑149 (MQ=255)
                                                                                                                                   gTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTg                >  2:3475254/1‑149 (MQ=255)
                                                                                                                                    tGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGc               <  2:4654844/149‑1 (MQ=255)
                                                                                                                                     gAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCa              >  1:3583938/1‑149 (MQ=255)
                                                                                                                                      aGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAg             <  2:2044181/149‑1 (MQ=255)
                                                                                                                                         tATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAg          >  2:2213137/1‑149 (MQ=255)
                                                                                                                                         tATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGGTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAg          >  1:2358051/1‑149 (MQ=255)
                                                                                                                                           ttCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCg        >  1:1157732/1‑149 (MQ=255)
                                                                                                                                                tCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGgaga   <  1:1749339/149‑1 (MQ=255)
                                                                                                                                                tCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATAGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCAGCGCGTATTGCAGGAGCGGgaga   >  2:2810436/1‑149 (MQ=255)
                                                                                                                                                 cATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGAt  <  1:42124/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGAT  >  CP009273/2606368‑2606661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑