Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,618,693 G→T 100% E74* (GAA→TAA)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,618,6930GT99.6% 896.5 / ‑4.4 262E74* (GAA→TAA) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (134/127);  total (134/128)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 262 total aligned reads displayed.

TGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGC  >  CP009273/2618547‑2618838
                                                                                                                                                  |                                                                                                                                                 
tGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTaa                                                                                                                                                 <  1:3822044/149‑1 (MQ=255)
tGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTaa                                                                                                                                                 >  2:3008447/1‑149 (MQ=255)
tGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTaa                                                                                                                                                 <  2:1584923/149‑1 (MQ=255)
     aTAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCt                                                                                                                                            <  2:1445426/149‑1 (MQ=255)
     aTAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCt                                                                                                                                            <  2:2173611/149‑1 (MQ=255)
       aGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTAc                                                                                                                                          >  1:3276197/1‑149 (MQ=255)
        gCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACa                                                                                                                                         >  1:232487/1‑149 (MQ=255)
          ccGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAg                                                                                                                                       >  2:2865659/1‑149 (MQ=255)
           cGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAgg                                                                                                                                      >  1:200295/1‑149 (MQ=255)
             tgtgGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGt                                                                                                                                    >  2:836119/1‑149 (MQ=255)
             tgtgGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGt                                                                                                                                    <  1:244326/149‑1 (MQ=255)
                 gTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTtc                                                                                                                                >  2:3247254/1‑149 (MQ=255)
                    gATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTcc                                                                                                                             <  1:81486/149‑1 (MQ=255)
                    gATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTcc                                                                                                                             <  2:2785782/149‑1 (MQ=255)
                               gCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCt                                                                                                                  >  2:2695925/1‑149 (MQ=255)
                                cAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg                                                                                                                 >  1:1339442/1‑149 (MQ=255)
                                 aGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGt                                                                                                                >  2:68513/1‑149 (MQ=255)
                                 aGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCGGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGt                                                                                                                <  2:1010043/149‑1 (MQ=255)
                                   attattGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAAGATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat                                                                                                              >  2:3938231/1‑149 (MQ=255)
                                    ttattGGCCGCCTGAAACAGCGGGTGCATCTGGCGGATGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtata                                                                                                             <  2:1614502/149‑1 (MQ=255)
                                     tattGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAg                                                                                                            <  1:2821599/149‑1 (MQ=255)
                                      attGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGt                                                                                                           <  2:2901562/149‑1 (MQ=255)
                                      attGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGt                                                                                                           <  2:340024/149‑1 (MQ=255)
                                      attGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGt                                                                                                           <  1:665341/149‑1 (MQ=255)
                                      attGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGt                                                                                                           <  1:2265379/149‑1 (MQ=255)
                                           ccgccTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTa                                                                                                      <  1:4161973/149‑1 (MQ=255)
                                              ccTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTAccc                                                                                                   <  1:3576643/149‑1 (MQ=255)
                                               cTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCa                                                                                                  <  2:3331640/149‑1 (MQ=255)
                                               cTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCa                                                                                                  <  2:508793/149‑1 (MQ=255)
                                                tGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTCTAGTTGGTACCCAt                                                                                                 >  2:1194072/1‑149 (MQ=255)
                                                 gAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATa                                                                                                <  2:771363/149‑1 (MQ=255)
                                                      aGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTg                                                                                           <  1:2765449/149‑1 (MQ=255)
                                                          ggTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTc                                                                                       <  1:279515/149‑1 (MQ=255)
                                                              cATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGgc                                                                                   <  2:600479/149‑1 (MQ=255)
                                                                       gACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGc                                                                          <  1:122087/149‑1 (MQ=255)
                                                                       gACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGc                                                                          >  1:3862348/1‑149 (MQ=255)
                                                                       gACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGc                                                                          <  2:2458971/149‑1 (MQ=255)
                                                                       gACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGc                                                                          >  2:747562/1‑149 (MQ=255)
                                                                             cTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGa                                                                    <  2:1261493/149‑1 (MQ=255)
                                                                               ggggCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACt                                                                  <  2:1871316/149‑1 (MQ=255)
                                                                                gggCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACtt                                                                 >  1:1546512/1‑149 (MQ=255)
                                                                                gggCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACtt                                                                 >  1:2064756/1‑149 (MQ=255)
                                                                                gggCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACtt                                                                 <  2:232610/149‑1 (MQ=255)
                                                                                 ggCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACttt                                                                <  1:445576/149‑1 (MQ=255)
                                                                                    cAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTg                                                             >  1:1283691/1‑149 (MQ=255)
                                                                                    cAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTg                                                             >  1:3734900/1‑149 (MQ=255)
                                                                                     agagactatGTTGAGTGAAGAGGCACTGACGCGCGGATTCACCGGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCCGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTCCTGa                                                            <  2:1594490/143‑1 (MQ=255)
                                                                                        taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAc                                                         <  2:3896091/149‑1 (MQ=255)
                                                                                          atatGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgc                                                       >  1:1920493/1‑149 (MQ=255)
                                                                                           tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcg                                                      >  1:4768886/1‑149 (MQ=255)
                                                                                           tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcg                                                      >  2:1731850/1‑149 (MQ=255)
                                                                                              gTTGAGTGAAGAGGAAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCgg                                                   >  2:2629232/1‑149 (MQ=255)
                                                                                               ttGAGTGCAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGa                                                  >  2:2526097/1‑149 (MQ=255)
                                                                                               ttGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGa                                                  <  2:2807097/149‑1 (MQ=255)
                                                                                               ttGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCAATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGa                                                  <  1:3297398/149‑1 (MQ=255)
                                                                                                 gAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGaaa                                                >  1:985114/1‑149 (MQ=255)
                                                                                                  aGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGaaaa                                               >  2:3694153/1‑149 (MQ=255)
                                                                                                      aaGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTc                                           <  1:1795553/149‑1 (MQ=255)
                                                                                                        gagGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCAt                                         <  1:1630698/149‑1 (MQ=255)
                                                                                                        gagGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCAt                                         <  2:1105194/149‑1 (MQ=255)
                                                                                                        gagGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCAt                                         >  1:4582015/1‑149 (MQ=255)
                                                                                                        gagGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCAt                                         >  1:398053/1‑149 (MQ=255)
                                                                                                         agGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCAtt                                        <  1:1376818/149‑1 (MQ=255)
                                                                                                          ggCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTc                                       <  1:2543516/149‑1 (MQ=255)
                                                                                                             aaTGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCt                                    >  1:3783148/1‑149 (MQ=255)
                                                                                                              aTGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTACCGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTa                                   <  2:2582182/149‑1 (MQ=255)
                                                                                                              aTGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTa                                   <  1:4053379/149‑1 (MQ=255)
                                                                                                                 aCGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTAccc                                >  1:2449889/1‑149 (MQ=255)
                                                                                                                 aCGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTAccc                                >  1:1497056/1‑149 (MQ=255)
                                                                                                                 aCGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTAccc                                <  1:4351050/149‑1 (MQ=255)
                                                                                                                      cgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTg                           <  1:4313954/149‑1 (MQ=255)
                                                                                                                       ggTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGa                          >  1:1091685/1‑149 (MQ=255)
                                                                                                                       ggTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGa                          <  2:544218/149‑1 (MQ=255)
                                                                                                                        gTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGACAAGGTCATTCCCTACCCGATTGaa                         >  2:2664694/1‑149 (MQ=255)
                                                                                                                        gTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGaa                         >  1:81103/1‑149 (MQ=255)
                                                                                                                        gTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGaa                         >  1:2673005/1‑149 (MQ=255)
                                                                                                                         tttAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGaaa                        <  2:1385297/149‑1 (MQ=255)
                                                                                                                         tttAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGaaa                        >  2:881713/1‑149 (MQ=255)
                                                                                                                         tttAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGaaa                        >  2:4674632/1‑149 (MQ=255)
                                                                                                                          ttAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAat                       <  1:2986812/149‑1 (MQ=255)
                                                                                                                          ttAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAat                       <  1:2586351/149‑1 (MQ=255)
                                                                                                                            aaaCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAatta                     <  1:878149/149‑1 (MQ=255)
                                                                                                                             aaCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAattat                    <  1:2923248/149‑1 (MQ=255)
                                                                                                                             aaCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAattat                    >  2:3643771/1‑149 (MQ=255)
                                                                                                                                tgtctgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTc                 >  1:368500/1‑149 (MQ=255)
                                                                                                                                  tctgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCg               >  1:1008746/1‑149 (MQ=255)
                                                                                                                                  tctgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCg               <  2:1820036/149‑1 (MQ=255)
                                                                                                                                  tctgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCg               <  2:4210454/149‑1 (MQ=255)
                                                                                                                                   ctgtcGCTGTTTGCCTAACGTCTACAAGGGTTTTCTCCTGCCAGAGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACACGATTGAAATTATTTCCgg              <  2:1359822/149‑1 (MQ=255)
                                                                                                                                    tgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGt             >  1:570698/1‑149 (MQ=255)
                                                                                                                                     gtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTa            >  2:1970945/1‑149 (MQ=255)
                                                                                                                                     gtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTa            >  1:3305561/1‑149 (MQ=255)
                                                                                                                                       cGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGACACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTCAt          >  2:4523018/1‑149 (MQ=255)
                                                                                                                                       cGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAAt          <  2:3104717/149‑1 (MQ=255)
                                                                                                                                        gCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATg         <  2:4093624/149‑1 (MQ=255)
                                                                                                                                        gCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATg         <  2:4318894/149‑1 (MQ=255)
                                                                                                                                           gTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATgaag      <  2:919175/149‑1 (MQ=255)
                                                                                                                                            tttGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATgaaga     <  2:232487/149‑1 (MQ=255)
                                                                                                                                               gCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGc  >  2:2830113/1‑149 (MQ=255)
                                                                                                                                               gCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGc  >  2:3233190/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGC  >  CP009273/2618547‑2618838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑