BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,626,858	T→A	5.5%	H191L (CAC→CTC)	guaB ←	IMP dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,626,858	0	T	A	5.5%	928.2 / 32.7	289	H191L (CAC→CTC)	guaB	IMP dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (141/132); new base A (6/10); total (147/142)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 295 total aligned reads displayed.

TGCGGCAACCAGCGCGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGTTCAGGTCGGTAAC  >  CP009273/2626710‑2627006

tGCGGCAACCAGCGCGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGt                                                                                                                                                      <  1:3713907/149‑1 (MQ=255)

 gCGGCAACCAGCGCGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTg                                                                                                                                                     <  2:2725418/149‑1 (MQ=255)

 gCGGCAACCAGCGCGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTg                                                                                                                                                     >  1:2431159/1‑149 (MQ=255)

  cGGCAACCAGCGCGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTgg                                                                                                                                                    <  2:3270281/149‑1 (MQ=255)

  cGGCAACCAGCGCGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTgg                                                                                                                                                    <  1:2551013/149‑1 (MQ=255)

    gCAACCAGCGCGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGaa                                                                                                                                                  <  1:1978666/149‑1 (MQ=255)

       aCCAGCGCGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACTGTGATCATGCCGATCAGGTGGAATTc                                                                                                                                               >  2:2262223/1‑149 (MQ=255)

         cAGCGCGTCAACCCGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGt                                                                                                                                             <  2:3588789/149‑1 (MQ=255)

          aGCGCGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGCATTCGtc                                                                                                                                            >  2:2645347/1‑149 (MQ=255)

          aGCGCGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGtc                                                                                                                                            >  1:2718422/1‑149 (MQ=255)

           gcgcGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGtca                                                                                                                                           <  2:1049753/149‑1 (MQ=255)

           gcgcGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGtca                                                                                                                                           <  2:1050218/149‑1 (MQ=255)

             gcgTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTACTGGAAGTCTTTCACGGTGATCAAGCCGATCAGGTGGAATTCGtcctc                                                                                                                                         >  1:2077517/1‑149 (MQ=255)

               gTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCaa                                                                                                                                       >  1:135045/1‑149 (MQ=255)

                tCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAac                                                                                                                                      <  1:391294/149‑1 (MQ=255)

                   acacGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAaccac                                                                                                                                   >  1:4712547/1‑149 (MQ=255)

                    cacGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAaccacc                                                                                                                                  <  1:4407760/149‑1 (MQ=255)

                     acGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAAccacca                                                                                                                                 <  1:3339332/149‑1 (MQ=255)

                      cGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAg                                                                                                                                >  1:1812618/1‑149 (MQ=255)

                        ctctTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAgcg                                                                                                                              >  2:423277/1‑149 (MQ=255)

                         tctTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGGTTACGGTCCGCGGTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCCTCAACCACCCgcgc                                                                                                                             >  1:3606037/1‑149 (MQ=255)

                          ctTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCt                                                                                                                            <  1:1421547/149‑1 (MQ=255)

                           ttCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCtt                                                                                                                           >  2:3680913/1‑149 (MQ=255)

                           ttCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCtt                                                                                                                           <  1:1066837/149‑1 (MQ=255)

                            tCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGAGGAATTCGTCATCAACCACCAGCGCttt                                                                                                                          >  2:94281/1‑149 (MQ=255)

                              gTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCttttt                                                                                                                        >  2:2488697/1‑149 (MQ=255)

                              gTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCttttt                                                                                                                        <  2:3298364/149‑1 (MQ=255)

                               ttACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTc                                                                                                                       <  2:3762350/149‑1 (MQ=255)

                               ttACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTc                                                                                                                       <  1:2940331/149‑1 (MQ=255)

                                  cccGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAAc                                                                                                                    <  2:4314948/149‑1 (MQ=255)

                                      cACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGt                                                                                                                <  2:4676399/149‑1 (MQ=255)

                                      cACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGt                                                                                                                <  2:518182/149‑1 (MQ=255)

                                         cTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGtttt                                                                                                             >  1:343787/1‑149 (MQ=255)

                                         cTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGtttt                                                                                                             <  1:721887/149‑1 (MQ=255)

                                          tGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGttttt                                                                                                            <  2:2471446/149‑1 (MQ=255)

                                          tGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGAGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGttttt                                                                                                            <  1:3162726/149‑1 (MQ=255)

                                                aaCCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCa                                                                                                      <  2:1738327/149‑1 (MQ=255)

                                                   cGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCAttt                                                                                                   <  1:3081389/149‑1 (MQ=255)

                                                     cTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGAGGAATTCGTCATCAACCACCAGCGCTTGTTCAACGCGTTTTTCGTGCAttttt                                                                                                 <  2:58246/149‑1 (MQ=255)

                                                      tGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTAAGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTAAACGCGTTTTTCGGGCATTTTTg                                                                                                >  1:137653/1‑149 (MQ=255)

                                                        cACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCCACGCGTTTTTCGTGCATTTTTGcc                                                                                              <  2:3211092/149‑1 (MQ=255)

                                                        cACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGcc                                                                                              <  2:3871998/149‑1 (MQ=255)

                                                                  cAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACtt                                                                                    >  2:1174428/1‑149 (MQ=255)

                                                                    gACGGCCTTGCTCGTCTTTACAGGCGTTCGGTGTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCa                                                                                  <  2:3917516/149‑1 (MQ=255)

                                                                     aCGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCAc                                                                                 <  1:3030124/149‑1 (MQ=255)

                                                                      cGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGTAAGTATTTAACGGTGATCATGCCGATAAGGTGGAATTCGTCATCAACCAACCGCGCATTTTCAACACGTTTTTAGTGCATTTTTTCAAGAACCACAACACg                                                                                >  1:2209754/1‑149 (MQ=255)

                                                                      cGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACg                                                                                <  1:1970595/149‑1 (MQ=255)

                                                                      cGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACg                                                                                <  1:1732826/149‑1 (MQ=255)

                                                                       ggCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTTCCAGCACCACATCACgg                                                                               >  1:3818138/1‑149 (MQ=255)

                                                                       ggCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACgg                                                                               >  2:779023/1‑149 (MQ=255)

                                                                        gCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAAAGCGTTTTTCGTGCCTTTTTGCCAGCACCACATAACggg                                                                              >  1:2228405/1‑149 (MQ=255)

                                                                        gCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCAAGCCGATAAGGTGGAAATCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCAATTTTTCCAGCACCACTTAACcgg                                                                              >  1:646714/1‑149 (MQ=255)

                                                                           ttGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGctt                                                                           >  2:2104265/1‑149 (MQ=255)

                                                                            tGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGcttc                                                                          >  1:4238971/1‑149 (MQ=255)

                                                                             gCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTGTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGcttca                                                                         >  2:4426285/1‑149 (MQ=255)

                                                                                cGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTAAAAGGGTTTTTAGTGCATTTTTTCCAGCACCACATCACGGGcttcacct                                                                      >  1:4653001/1‑149 (MQ=255)

                                                                                 gTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACATCACGGGcttcaccat                                                                     >  1:1006437/1‑147 (MQ=255)

                                                                                  tCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGcttcaccttc                                                                    <  2:2405883/149‑1 (MQ=255)

                                                                                       aCAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTcacgca                                                               <  2:3116327/149‑1 (MQ=255)

                                                                                             gTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGa                                                         <  2:293735/149‑1 (MQ=255)

                                                                                             gTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGa                                                         <  2:4570809/149‑1 (MQ=255)

                                                                                              ttCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGAc                                                        >  2:4721642/1‑149 (MQ=255)

                                                                                              ttCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCATCACGCACGGTGAc                                                        >  1:2115746/1‑149 (MQ=255)

                                                                                                  gTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGa                                                    <  2:1146083/149‑1 (MQ=255)

                                                                                                      aCGTTCCGCTTTCTGGAAGTCTTTAACGGTGATCATGCCGATAAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTTCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACCCACGGTGACCAGACGct                                                >  1:1750484/1‑149 (MQ=255)

                                                                                                        gTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCCACGCGTTTTTAGTGCATTTTTTCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGctct                                              >  1:589605/1‑149 (MQ=255)

                                                                                                        gTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGctct                                              >  1:1337766/1‑149 (MQ=255)

                                                                                                         ttCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCtt                                             >  2:2015197/1‑149 (MQ=255)

                                                                                                           ccGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTc                                           <  2:3340723/149‑1 (MQ=255)

                                                                                                             gCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCgg                                         <  1:1697231/149‑1 (MQ=255)

                                                                                                               tttCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCg                                       >  1:1615246/1‑149 (MQ=255)

                                                                                                               tttCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCg                                       >  1:1454353/1‑149 (MQ=255)

                                                                                                               tttCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCg                                       >  2:3059985/1‑149 (MQ=255)

                                                                                                                 tCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGAGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTc                                     <  2:2695409/149‑1 (MQ=255)

                                                                                                                 tCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGAGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTc                                     <  1:1792323/149‑1 (MQ=255)

                                                                                                                   tGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCAt                                   >  1:3258474/1‑149 (MQ=255)

                                                                                                                    ggAAGTCTTTCACGGTGATCATGCCGATCAGGAGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATg                                  >  1:2342761/1‑149 (MQ=255)

                                                                                                                      aaGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTa                                >  1:1275024/1‑149 (MQ=255)

                                                                                                                       aGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTaa                               >  2:3700776/1‑149 (MQ=255)

                                                                                                                        gTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTaaa                              >  2:1409544/1‑149 (MQ=255)

                                                                                                                           tttCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGc                           <  2:3430496/149‑1 (MQ=255)

                                                                                                                             tCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTa                         >  1:4000432/1‑149 (MQ=255)

                                                                                                                              cACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTaa                        >  1:3002329/1‑149 (MQ=255)

                                                                                                                               aCGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAAc                       >  2:6704/1‑149 (MQ=255)

                                                                                                                               aCGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAAc                       >  2:4589060/1‑149 (MQ=255)

                                                                                                                                  gTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCgg                    >  2:3498042/1‑149 (MQ=255)

                                                                                                                                   tGATCATGCCGATCAGGAGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGc                   <  2:691473/149‑1 (MQ=255)

                                                                                                                                   tGATCATGCCGATCAGGAGGAATTCGTCATCAACCACCAGCGCTGTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGc                   <  2:726218/149‑1 (MQ=255)

                                                                                                                                    gATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCt                  >  2:1119024/1‑149 (MQ=255)

                                                                                                                                     aTCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTg                 >  1:3007930/1‑149 (MQ=255)

                                                                                                                                     aTCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTg                 >  1:3007911/1‑149 (MQ=255)

                                                                                                                                      tCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTgg                >  1:1561015/1‑149 (MQ=255)

                                                                                                                                       cATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGt               >  2:955584/1‑149 (MQ=255)

                                                                                                                                        aTGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGtt              >  1:619530/1‑149 (MQ=255)

                                                                                                                                          gCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGTTCa            <  2:4507363/149‑1 (MQ=255)

                                                                                                                                            cGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGTTCAgg          >  1:1176353/1‑149 (MQ=255)

                                                                                                                                             gATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGTTCAGGt         <  2:1617902/149‑1 (MQ=255)

                                                                                                                                                 aGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGTTCAGGTCGGt     <  1:933689/149‑1 (MQ=255)

                                                                                                                                                  ggTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGTTCAGGTCGGTa    <  1:2857699/149‑1 (MQ=255)

                                                                                                                                                    tGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGTTCAGGTCGGTAAc  <  1:803537/149‑1 (MQ=255)

TGCGGCAACCAGCGCGTCAACACGCTCTTCGTTACCCGCACCTGCGCCAACCGCTGCACCAACACGCAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGCCAGCACCACTTCACGGGCTTCACCTTCACGCACGGTGACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGTTCAGGTCGGTAAC  >  CP009273/2626710‑2627006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`