Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,651,665 G→T 100% G210G (GGC→GGA)  hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,651,6650GT99.4% 1126.9 / ‑1.9 332G210G (GGC→GGA) hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (194/136);  total (196/136)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 332 total aligned reads displayed.

CTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCAT  >  CP009273/2651519‑2651810
                                                                                                                                                  |                                                                                                                                                 
cTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTcc                                                                                                                                                 >  2:1007615/1‑149 (MQ=255)
   cGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCAcc                                                                                                                                              <  1:2306156/149‑1 (MQ=255)
   cGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCAcc                                                                                                                                              >  1:3876393/1‑149 (MQ=255)
    gATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCg                                                                                                                                             >  1:3460747/1‑149 (MQ=255)
        aGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGt                                                                                                                                         >  2:4436350/1‑149 (MQ=255)
          gAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCa                                                                                                                                       <  1:2586541/149‑1 (MQ=255)
          gAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCa                                                                                                                                       >  1:4257392/1‑149 (MQ=255)
          gAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCa                                                                                                                                       >  1:4271433/1‑149 (MQ=255)
            aTGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATa                                                                                                                                     >  2:373336/1‑149 (MQ=255)
              gCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATaaa                                                                                                                                   >  1:4025057/1‑149 (MQ=255)
               cccGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACCCGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAAc                                                                                                                                  <  1:1256161/149‑1 (MQ=255)
               cccGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAAc                                                                                                                                  <  2:4655128/149‑1 (MQ=255)
                 cGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACgg                                                                                                                                <  1:2749797/149‑1 (MQ=255)
                   ccTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCg                                                                                                                              <  2:3369338/149‑1 (MQ=255)
                    gtGCTCGCGAATGTAATCCGCCAGCTGATGGTCGAAATAATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGAAATTCAAACACGCCGCTAATTAAGCGCATAATGTAAATATAAAACGTCCCTCCACCGTGGTAATAAACGGCGa                                                                                                                             <  2:3000065/148‑1 (MQ=255)
                    cTGCTCGCTATTGTAATCCGCCAGCAGTTGGTCGAAAACATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGa                                                                                                                             <  2:3065898/149‑1 (MQ=255)
                     tGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCTGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGAt                                                                                                                            <  2:1168966/149‑1 (MQ=255)
                     tGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGAt                                                                                                                            >  1:2443335/1‑149 (MQ=255)
                     tGCTCGCGAATGTAATCCGCCAGCAGATGGGCGACATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGAt                                                                                                                            <  2:1168851/149‑1 (MQ=255)
                      gCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATc                                                                                                                           <  2:4220832/149‑1 (MQ=255)
                       cTCGCTAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGTAATCACCGCGGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCATAATGGAAATATCAACCGTCCCTCCACCGAGGTCATAAACGGCGATCa                                                                                                                          <  1:3402850/149‑1 (MQ=255)
                       cTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGACTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAACTATCAAACGTCCCTCCACCGAGGTCATCAACGGCGATCa                                                                                                                          >  2:2557694/1‑149 (MQ=255)
                       cTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCa                                                                                                                          >  2:165632/1‑149 (MQ=255)
                          gcgAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGc                                                                                                                       <  2:4159388/149‑1 (MQ=255)
                          gcgAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGc                                                                                                                       >  1:794994/1‑149 (MQ=255)
                           cgAATGTAATCCGCCAGCTGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGcc                                                                                                                      <  1:707928/149‑1 (MQ=255)
                             aaTGTAATCCGCCTGCTTTGGGTCTAAATCATCGCCGCCGAGCGGGTAATCACCGCGGTTTGCCAGCACTTCAAACACGCCGCGAATTAAGCGCATAATGTAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCtt                                                                                                                    <  1:2207779/149‑1 (MQ=255)
                             aaTGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCtt                                                                                                                    <  2:2367600/149‑1 (MQ=255)
                             aaTGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCtt                                                                                                                    >  1:3275013/1‑149 (MQ=255)
                                  aaTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGa                                                                                                               >  1:4569524/1‑149 (MQ=255)
                                   aTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGACATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAc                                                                                                              >  2:812844/1‑149 (MQ=255)
                                              gagggTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGc                                                                                                   <  2:1455584/146‑1 (MQ=255)
                                              gATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGc                                                                                                   >  2:1309669/1‑149 (MQ=255)
                                              gATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGc                                                                                                   >  1:3590388/1‑149 (MQ=255)
                                                    cGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAg                                                                                             <  2:1535427/149‑1 (MQ=255)
                                                       aaTCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCg                                                                                          >  2:3866867/1‑149 (MQ=255)
                                                         tcatcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAt                                                                                        >  2:4048073/1‑149 (MQ=255)
                                                           atcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAg                                                                                      >  2:2143095/1‑149 (MQ=255)
                                                            tcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAACCACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGc                                                                                     <  2:3646669/149‑1 (MQ=255)
                                                            tcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGc                                                                                     >  1:4037340/1‑149 (MQ=255)
                                                                  ccgAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGc                                                                               <  2:1990499/149‑1 (MQ=255)
                                                                      gcgcGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtc                                                                           <  2:3559739/149‑1 (MQ=255)
                                                                          ggAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGtt                                                                       <  1:1594333/149‑1 (MQ=255)
                                                                          ggAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGtt                                                                       <  2:4260519/149‑1 (MQ=255)
                                                                             aTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                                    >  2:3845404/1‑149 (MQ=255)
                                                                                aCCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaa                                                                 <  2:2270097/149‑1 (MQ=255)
                                                                                 ccgccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaag                                                                >  2:4780195/1‑149 (MQ=255)
                                                                                     cgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagtaag                                                            >  2:1818547/1‑149 (MQ=255)
                                                                                     cgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagtaag                                                            <  2:4121440/149‑1 (MQ=255)
                                                                                       gTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAgcg                                                          >  1:3716621/1‑149 (MQ=255)
                                                                                       gTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAgcg                                                          >  2:4618026/1‑149 (MQ=255)
                                                                                              gCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACg                                                   >  2:821862/1‑149 (MQ=255)
                                                                                                aCTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGTTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGGCGGTTCGTTAAGTAAGCGCAGGACGTg                                                 >  2:3328330/1‑149 (MQ=255)
                                                                                                aCTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTg                                                 >  1:4621850/1‑149 (MQ=255)
                                                                                                aCTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTg                                                 >  1:486638/1‑149 (MQ=255)
                                                                                                aCTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTg                                                 >  2:1925436/1‑149 (MQ=255)
                                                                                                aCTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGACCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTg                                                 >  1:3447583/1‑149 (MQ=255)
                                                                                                 cTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGa                                                >  1:1378859/1‑149 (MQ=255)
                                                                                                  ttCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGaa                                               >  2:1620461/1‑149 (MQ=255)
                                                                                                  ttCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGGTAAGTAAGCGCAGGACGTGaa                                               >  2:4003832/1‑149 (MQ=255)
                                                                                                   tCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCACACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAg                                              <  2:4519732/149‑1 (MQ=255)
                                                                                                   tCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAg                                              <  1:4185903/149‑1 (MQ=255)
                                                                                                    cAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAgg                                             <  1:259977/149‑1 (MQ=255)
                                                                                                    cAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAgg                                             <  2:1724405/149‑1 (MQ=255)
                                                                                                    cAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAgg                                             <  1:2652692/149‑1 (MQ=255)
                                                                                                     aaaCACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGc                                            <  2:687891/149‑1 (MQ=255)
                                                                                                      aaCACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGcc                                           >  1:3921705/1‑149 (MQ=255)
                                                                                                         acGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGc                                        >  1:4231994/1‑149 (MQ=255)
                                                                                                                   ttAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGcgcc                              <  1:594500/149‑1 (MQ=255)
                                                                                                                     aaGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGcgccgc                            >  1:898067/1‑149 (MQ=255)
                                                                                                                     aaGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGcgccgc                            <  1:1706893/149‑1 (MQ=255)
                                                                                                                      aGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCcgcg                           >  2:1003687/1‑149 (MQ=255)
                                                                                                                      aGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCcgcg                           >  1:6920/1‑149 (MQ=255)
                                                                                                                       gcgcAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGt                          <  1:2620963/149‑1 (MQ=255)
                                                                                                                        cgcAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTc                         <  1:3327061/149‑1 (MQ=255)
                                                                                                                         gcAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCt                        <  2:647753/149‑1 (MQ=255)
                                                                                                                           aGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCttt                      >  1:2191425/1‑149 (MQ=255)
                                                                                                                           aGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCttt                      >  1:1041603/1‑149 (MQ=255)
                                                                                                                            gAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTg                     >  2:3273096/1‑149 (MQ=255)
                                                                                                                            gAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTg                     >  2:1251048/1‑149 (MQ=255)
                                                                                                                              aTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGt                   >  2:2141413/1‑149 (MQ=255)
                                                                                                                               tGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTg                  >  1:2849424/1‑149 (MQ=255)
                                                                                                                                 gAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGcc                >  1:1531302/1‑149 (MQ=255)
                                                                                                                                 gAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGcc                <  2:2921516/149‑1 (MQ=255)
                                                                                                                                  aaaTATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGccc               >  2:575628/1‑149 (MQ=255)
                                                                                                                                  aaaTATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGccc               <  1:4644531/149‑1 (MQ=255)
                                                                                                                                   aaTATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCt              <  2:955958/149‑1 (MQ=255)
                                                                                                                                    atatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTg             >  1:121772/1‑149 (MQ=255)
                                                                                                                                    atatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTCCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCGTTGGTGCCCTg             >  2:2781893/1‑149 (MQ=255)
                                                                                                                                     tatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGa            <  2:3500499/149‑1 (MQ=255)
                                                                                                                                     tatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGa            >  1:3148040/1‑149 (MQ=255)
                                                                                                                                      atCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGAc           >  2:2732056/1‑149 (MQ=255)
                                                                                                                                       tCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTGTGGTGCCCTGACg          >  2:1092514/1‑149 (MQ=255)
                                                                                                                                         aaaCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCt        >  2:3205281/1‑149 (MQ=255)
                                                                                                                                         aaaCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCt        <  1:2523570/149‑1 (MQ=255)
                                                                                                                                          aaCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTg       >  2:129969/1‑149 (MQ=255)
                                                                                                                                          aaCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTg       <  1:4373097/149‑1 (MQ=255)
                                                                                                                                               cccTCCTCCGAGGTCATAATCGGCGTTCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCTATAGCCGCAGCGGTCGGTTCGTTTAGTTTGCGCAGGACGTGATGGCCCGCCAGACGCGCCGCGTATTTGGTGCCCTGACGCTGGGCAt  <  2:4376084/149‑1 (MQ=255)
                                                                                                                                               cccTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCAt  >  2:3834902/1‑149 (MQ=255)
                                                                                                                                               cccTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCAt  <  1:2251540/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCAT  >  CP009273/2651519‑2651810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑