Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 232,860 G→T 100% D103Y (GAT→TAT)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273232,8600GT100.0% 1016.7 / NA 290D103Y (GAT→TAT) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (138/152);  total (138/152)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 292 total aligned reads displayed.

CCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCG  >  CP009273/232714‑233005
                                                                                                                                                  |                                                                                                                                                 
 cGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCtata                                                                                                                                                <  1:4706806/149‑1 (MQ=255)
  gATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTatat                                                                                                                                               >  2:172730/1‑149 (MQ=255)
  gATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTatat                                                                                                                                               >  2:691886/1‑149 (MQ=255)
  gATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGGAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTatat                                                                                                                                               <  1:2430999/149‑1 (MQ=255)
    tCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCg                                                                                                                                             >  2:3650547/1‑149 (MQ=255)
    tCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCg                                                                                                                                             >  2:1259806/1‑149 (MQ=255)
        cTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCtt                                                                                                                                         <  2:1626459/149‑1 (MQ=255)
        cTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCtt                                                                                                                                         <  1:3954842/149‑1 (MQ=255)
         tggtggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCttt                                                                                                                                        >  2:759466/1‑149 (MQ=255)
         tggtggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCttt                                                                                                                                        <  2:663108/149‑1 (MQ=255)
         tggtggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCttt                                                                                                                                        >  1:726918/1‑149 (MQ=255)
          ggtggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTa                                                                                                                                       <  1:3861762/149‑1 (MQ=255)
            tggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATg                                                                                                                                     <  2:707702/149‑1 (MQ=255)
            tggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATg                                                                                                                                     <  1:4642687/149‑1 (MQ=255)
             ggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATgg                                                                                                                                    <  1:2308081/149‑1 (MQ=255)
                tCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACt                                                                                                                                 <  1:4571273/149‑1 (MQ=255)
                    gAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGa                                                                                                                             >  2:3262481/1‑149 (MQ=255)
                    gAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGa                                                                                                                             <  1:3272553/149‑1 (MQ=255)
                      aGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGt                                                                                                                           <  1:743042/149‑1 (MQ=255)
                        ccTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGttt                                                                                                                         <  1:1926349/149‑1 (MQ=255)
                        ccTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGttt                                                                                                                         <  1:1995804/149‑1 (MQ=255)
                         cTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGtttt                                                                                                                        >  1:35554/1‑149 (MQ=255)
                         cTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGtttt                                                                                                                        <  2:3511795/149‑1 (MQ=255)
                          tttGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTc                                                                                                                       <  1:2777008/149‑1 (MQ=255)
                          tttGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTc                                                                                                                       <  2:1533787/149‑1 (MQ=255)
                          tttGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTc                                                                                                                       <  1:668698/149‑1 (MQ=255)
                          tttGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTc                                                                                                                       >  1:298056/1‑149 (MQ=255)
                             ggCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGtt                                                                                                                    >  1:3436300/1‑149 (MQ=255)
                             ggCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGtt                                                                                                                    <  1:3762571/149‑1 (MQ=255)
                              gCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTg                                                                                                                   >  2:4270548/1‑149 (MQ=255)
                                gTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCt                                                                                                                 <  1:2576414/149‑1 (MQ=255)
                                gTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCt                                                                                                                 <  1:1659756/149‑1 (MQ=255)
                                   cATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCttta                                                                                                              <  1:3970683/149‑3 (MQ=255)
                                    aTGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAg                                                                                                             >  1:321721/1‑149 (MQ=255)
                                       gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                                                                                                          <  2:1826051/149‑1 (MQ=255)
                                          ttGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGat                                                                                                       <  2:3339844/149‑1 (MQ=255)
                                            gCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGatat                                                                                                     <  2:4595487/149‑1 (MQ=255)
                                              cGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTc                                                                                                   <  2:1604994/149‑1 (MQ=255)
                                                 tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa                                                                                                >  2:4779807/1‑149 (MQ=255)
                                                 tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa                                                                                                >  1:433377/1‑149 (MQ=255)
                                                  gAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGaa                                                                                               <  2:2964307/149‑1 (MQ=255)
                                                  gAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGaa                                                                                               >  2:212617/1‑149 (MQ=255)
                                                  gAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGaa                                                                                               <  2:4622114/149‑1 (MQ=255)
                                                     tttttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAc                                                                                            >  1:482903/1‑149 (MQ=255)
                                                      ttttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAcc                                                                                           <  1:3645815/149‑1 (MQ=255)
                                                      ttttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAcc                                                                                           <  1:4234948/149‑1 (MQ=255)
                                                      ttttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAcc                                                                                           <  2:1678522/149‑1 (MQ=255)
                                                         tGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAAt                                                                                        <  2:3101726/149‑1 (MQ=255)
                                                          gCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATa                                                                                       >  2:1406564/1‑149 (MQ=255)
                                                          gCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATa                                                                                       >  2:2674679/1‑149 (MQ=255)
                                                          gCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATa                                                                                       <  1:1123948/149‑1 (MQ=255)
                                                           cTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATAc                                                                                      <  1:304421/149‑1 (MQ=255)
                                                           cTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATAc                                                                                      <  1:1540991/149‑1 (MQ=255)
                                                               aTAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTc                                                                                  <  1:4356129/149‑1 (MQ=255)
                                                               aTAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTc                                                                                  <  2:2311824/149‑1 (MQ=255)
                                                                tAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCt                                                                                 <  2:241607/149‑1 (MQ=255)
                                                                  aGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGt                                                                               <  1:629155/149‑1 (MQ=255)
                                                                   gCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTa                                                                              <  2:2802955/149‑1 (MQ=255)
                                                                     ccACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAg                                                                            >  2:494318/1‑149 (MQ=255)
                                                                      cACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAgg                                                                           >  1:1027788/1‑149 (MQ=255)
                                                                      cACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAgg                                                                           >  1:2571153/1‑149 (MQ=255)
                                                                        cGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTc                                                                         >  1:4098112/1‑149 (MQ=255)
                                                                          tttGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAc                                                                       <  2:196183/149‑1 (MQ=255)
                                                                          tttGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAc                                                                       >  2:563085/1‑149 (MQ=255)
                                                                          tttGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAc                                                                       >  2:2441111/1‑149 (MQ=255)
                                                                          tttGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAc                                                                       <  2:493446/149‑1 (MQ=255)
                                                                          tttGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAc                                                                       <  2:3741557/149‑1 (MQ=255)
                                                                            tGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCg                                                                     <  2:4460659/149‑1 (MQ=255)
                                                                                gAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAg                                                                 <  2:4087944/149‑1 (MQ=255)
                                                                                   gTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCt                                                              <  1:1009392/149‑1 (MQ=255)
                                                                                     aGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTg                                                            >  1:1808717/1‑149 (MQ=255)
                                                                                     aGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTg                                                            >  1:923350/1‑149 (MQ=255)
                                                                                          aTGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTg                                                       >  1:4521603/1‑149 (MQ=255)
                                                                                               ttCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTCGCGGTGGCGAg                                                  <  2:2331133/149‑1 (MQ=255)
                                                                                                  aTGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGaa                                               >  1:4655006/1‑149 (MQ=255)
                                                                                                    ggACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGaaaa                                             <  2:831803/149‑1 (MQ=255)
                                                                                                      aCTATAGTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATg                                           <  1:2696759/149‑1 (MQ=255)
                                                                                                       cTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTGTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGt                                          <  2:4382726/149‑1 (MQ=255)
                                                                                                          tatTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTc                                       <  2:2253742/149‑1 (MQ=255)
                                                                                                          tatTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTc                                       <  2:2703297/149‑1 (MQ=255)
                                                                                                           atTCGCGGCGCGGAGTTGGTGATCCATACCGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTcc                                      <  2:3459434/149‑1 (MQ=255)
                                                                                                               gcggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGt                                  <  1:1259806/149‑1 (MQ=255)
                                                                                                               gcggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGt                                  <  1:3650547/149‑1 (MQ=255)
                                                                                                                 ggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTaa                                >  2:1906195/1‑149 (MQ=255)
                                                                                                                 ggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTaa                                >  2:3920683/1‑149 (MQ=255)
                                                                                                                  gcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAg                               >  2:632942/1‑149 (MQ=255)
                                                                                                                   cgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAgc                              <  1:852876/149‑1 (MQ=255)
                                                                                                                       gAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATTGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCaa                          <  2:1540656/149‑1 (MQ=255)
                                                                                                                       gAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCaa                          >  2:2046614/1‑149 (MQ=255)
                                                                                                                       gAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCaa                          >  1:1432809/1‑149 (MQ=255)
                                                                                                                        aGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAAc                         <  2:3149441/149‑1 (MQ=255)
                                                                                                                        aGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAAc                         <  2:1183813/149‑1 (MQ=255)
                                                                                                                                aTCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGa                 >  2:3036273/1‑149 (MQ=255)
                                                                                                                                aTCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGa                 <  2:4013645/149‑1 (MQ=255)
                                                                                                                                    aTAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCg             <  1:2854383/149‑1 (MQ=255)
                                                                                                                                     tAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGt            >  1:2066212/1‑149 (MQ=255)
                                                                                                                                         gCAGCGTTCTATCTCGGCTTGATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTAtg        <  2:3329106/149‑1 (MQ=255)
                                                                                                                                           aGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTAtgtg      >  2:2003703/1‑149 (MQ=255)
                                                                                                                                             cGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCt    <  2:4722086/149‑1 (MQ=255)
                                                                                                                                               ttCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCg  >  1:31077/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCG  >  CP009273/232714‑233005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑