Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,681,873 T→A 100% Q97L (CAG→CTG)  glrR ← response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,681,8730TA99.6% 928.8 / ‑4.6 271Q97L (CAG→CTG) glrRresponse regulator regulating glmY sRNA in two‑component system with sensor protein GlrK
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (131/138);  minor base G (0/1);  total (132/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 280 total aligned reads displayed.

AACGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCTGATGACTAAATCTACTTT  >  CP009273/2681727‑2682018
                                                                                                                                                  |                                                                                                                                                 
aaCGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCagt                                                                                                                                                 >  2:2241611/1‑149 (MQ=255)
 aCGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCagtg                                                                                                                                                >  2:2612167/1‑149 (MQ=255)
 aCGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGATTGGTGAGGAAACTAAAAACGCCCTGCagtg                                                                                                                                                <  1:2671936/149‑1 (MQ=255)
    cagcaTCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTg                                                                                                                                             <  1:3935119/149‑1 (MQ=255)
      gcaTCAGCGGGCTGCGGGTGACAAGTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTgca                                                                                                                                           <  2:4082972/149‑1 (MQ=255)
        aTCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTCTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTgcagc                                                                                                                                         >  1:3296287/1‑149 (MQ=255)
         tCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTgcagca                                                                                                                                        >  1:1218377/1‑149 (MQ=255)
          ccgCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCaa                                                                                                                                       <  2:1601039/147‑1 (MQ=255)
          cAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCaa                                                                                                                                       <  2:4674421/149‑1 (MQ=255)
           aGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAACCTAAAAACGCCCTGCAGTGTTGCAGCAAc                                                                                                                                      >  2:1719938/1‑149 (MQ=255)
           aGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAAc                                                                                                                                      >  1:1434097/1‑149 (MQ=255)
                gCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCAt                                                                                                                                 <  1:894908/149‑1 (MQ=255)
                    cGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGAATCGTAAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCggg                                                                                                                             <  1:88323/149‑1 (MQ=255)
                     gggTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGa                                                                                                                            >  2:3925772/1‑149 (MQ=255)
                      ggTGACCATGGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGaa                                                                                                                           <  2:196689/149‑1 (MQ=255)
                       gTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAAt                                                                                                                          >  2:1591348/1‑149 (MQ=255)
                       gTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAAt                                                                                                                          >  2:1260714/1‑149 (MQ=255)
                       gTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAAt                                                                                                                          <  1:66723/149‑1 (MQ=255)
                       gTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAAt                                                                                                                          >  2:3484767/1‑149 (MQ=255)
                        tGACATTTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATa                                                                                                                         <  1:444270/149‑1 (MQ=255)
                        tGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATa                                                                                                                         <  2:928180/149‑1 (MQ=255)
                         gACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAACCTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAg                                                                                                                        >  2:3701335/1‑149 (MQ=255)
                         gACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAg                                                                                                                        <  2:4775179/149‑1 (MQ=255)
                         gACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAg                                                                                                                        >  2:3701282/1‑149 (MQ=255)
                           ctaTTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGTATTGCTCCAGCGCATCGTCATTTGCCTGATATAGCGCGTCTTTGTCGACTGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGaa                                                                                                                      <  1:307551/147‑1 (MQ=255)
                           cAATTGCCTCGCGCCAGCGTTCATCGGGGGCTGGCGCGGATTGCTCCAGCGCATCGTCAAGTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGaa                                                                                                                      <  1:2484304/149‑1 (MQ=255)
                            aaTTGCCTCGCGCCAGCGTTCATGGGGGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCGGTGTTGCAGCAACGGCATCGGGAATAGAAc                                                                                                                     <  1:534295/149‑1 (MQ=255)
                                gCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATg                                                                                                                 <  1:2984642/149‑1 (MQ=255)
                                gCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATg                                                                                                                 >  2:4155310/1‑149 (MQ=255)
                                 ccTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATgc                                                                                                                <  1:1328534/149‑1 (MQ=255)
                                    cgcgCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCGTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGcgcg                                                                                                             >  2:815948/1‑149 (MQ=255)
                                     gcgcCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCgg                                                                                                            >  1:2319567/1‑149 (MQ=255)
                                       gcCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGAAATAGAACCATGCGCGGTa                                                                                                          <  2:1297253/149‑1 (MQ=255)
                                        ccAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTaa                                                                                                         >  1:4022066/1‑149 (MQ=255)
                                        ccAGCGTTCATCGGTGGCTGGCGCGGAGTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTaa                                                                                                         <  2:3782773/149‑1 (MQ=255)
                                          aGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGa                                                                                                       <  2:1440992/149‑1 (MQ=255)
                                           gCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGaa                                                                                                      <  1:4143316/149‑1 (MQ=255)
                                                  tCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTAc                                                                                               >  1:4577021/1‑149 (MQ=255)
                                                    ggTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAACCTAAAAACGCCCTGCAGTGGTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTCAGAATAATTACCg                                                                                             >  2:3984557/1‑149 (MQ=255)
                                                    ggTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCg                                                                                             >  1:2740894/1‑149 (MQ=255)
                                                        gctggcGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCAt                                                                                         <  2:2350755/149‑1 (MQ=255)
                                                           ggcGCGGAGTGCGCCAGCGCATCGTCAATGGCCTGAGAGCGCGCGTCGTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTcc                                                                                      <  2:3058965/149‑1 (MQ=255)
                                                            gcgcGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTccc                                                                                     <  2:3099844/149‑1 (MQ=255)
                                                            gcgcGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTccc                                                                                     >  1:2247931/1‑149 (MQ=255)
                                                                 gATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTg                                                                                >  2:256889/1‑149 (MQ=255)
                                                                    tGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCAc                                                                             <  2:3567737/149‑1 (MQ=255)
                                                                      cTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACtt                                                                           <  2:609311/149‑1 (MQ=255)
                                                                      cTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACtt                                                                           <  1:1821112/149‑1 (MQ=255)
                                                                      cTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACtt                                                                           <  1:1936298/149‑1 (MQ=255)
                                                                        ccAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTc                                                                         <  1:4250244/149‑1 (MQ=255)
                                                                        ccAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTc                                                                         >  1:986269/1‑149 (MQ=255)
                                                                         cAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCt                                                                        <  1:1680349/149‑1 (MQ=255)
                                                                          aGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTg                                                                       <  1:1401295/149‑1 (MQ=255)
                                                                           gcgcATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTgg                                                                      >  2:730097/1‑149 (MQ=255)
                                                                              cATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGAtt                                                                   >  1:1083745/1‑149 (MQ=255)
                                                                                       ttGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAAc                                                          >  2:3672733/1‑149 (MQ=255)
                                                                                           cTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCt                                                      >  2:1768384/1‑149 (MQ=255)
                                                                                             gATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGc                                                    >  2:1518299/1‑149 (MQ=255)
                                                                                              atatAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCa                                                   >  2:1038178/1‑149 (MQ=255)
                                                                                                ataGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATa                                                 <  2:1320728/149‑1 (MQ=255)
                                                                                                  aGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATAcc                                               >  1:2225004/1‑149 (MQ=255)
                                                                                                  aGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATAcc                                               >  1:236387/1‑149 (MQ=255)
                                                                                                   gcgcGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCg                                              >  1:1259853/1‑149 (MQ=255)
                                                                                                       gTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCa                                          <  1:2050840/149‑1 (MQ=255)
                                                                                                        tCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCAt                                         >  1:2171318/1‑149 (MQ=255)
                                                                                                         cTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCAtt                                        <  1:3326203/149‑1 (MQ=255)
                                                                                                         cTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCAtt                                        <  2:2545776/149‑1 (MQ=255)
                                                                                                         cTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCAtt                                        <  2:193939/149‑1 (MQ=255)
                                                                                                         cTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCAtt                                        <  1:4365507/149‑1 (MQ=255)
                                                                                                          tttGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCAttt                                       >  2:2104779/1‑149 (MQ=255)
                                                                                                          tttGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCAttt                                       >  2:4503282/1‑149 (MQ=255)
                                                                                                          tttGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAACAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCAttt                                       >  1:642478/1‑149 (MQ=255)
                                                                                                            tGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTca                                     <  1:3925772/149‑1 (MQ=255)
                                                                                                                gACAGGCTTGTTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGTTAAGAATAAGTACCGGCATTCCCGGCTGCAATTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTcatcca                                 <  1:1318572/149‑1 (MQ=255)
                                                                                                                 aCAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTcatccat                                >  2:2065258/1‑149 (MQ=255)
                                                                                                                  cAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTcatccatc                               <  2:522400/149‑1 (MQ=255)
                                                                                                                  cAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTcatccatc                               >  1:4759315/1‑149 (MQ=255)
                                                                                                                  cAGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTcatccatc                               >  1:4759234/1‑149 (MQ=255)
                                                                                                                   aGGCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCatccatcc                              <  2:3161920/149‑1 (MQ=255)
                                                                                                                    ggCTTGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCg                             >  2:2658986/1‑149 (MQ=255)
                                                                                                                       ttGGTGAGGAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAg                          >  1:1524531/1‑149 (MQ=255)
                                                                                                                              ggAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCtg                   <  1:4498889/149‑1 (MQ=255)
                                                                                                                              ggAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCtg                   >  1:714247/1‑149 (MQ=255)
                                                                                                                              ggAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCtg                   <  1:54378/149‑1 (MQ=255)
                                                                                                                              ggAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCtg                   <  1:1913901/149‑1 (MQ=255)
                                                                                                                               gAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCtga                  >  1:4098870/1‑149 (MQ=255)
                                                                                                                               gAAACTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCtga                  <  2:1217388/149‑1 (MQ=255)
                                                                                                                                aaaCTAAACACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCCTGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCtgat                 <  2:3296287/149‑1 (MQ=255)
                                                                                                                                 aaCTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCtgatg                <  2:673279/149‑1 (MQ=255)
                                                                                                                                 aaCTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCtgatg                <  2:672945/149‑1 (MQ=255)
                                                                                                                                 aaCTAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCtgatg                >  2:587730/1‑149 (MQ=255)
                                                                                                                                   ctaccaaCGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCAGTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCTGATGAc              <  2:3351864/144‑1 (MQ=255)
                                                                                                                                    tAAAAACGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCTGATGACt             <  1:2703461/149‑1 (MQ=255)
                                                                                                                                        aaCGCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCTGATGACTAAAt         <  1:3636777/149‑1 (MQ=255)
                                                                                                                                           gCCCTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCTGATGACTAAATCTa      <  1:1021946/149‑1 (MQ=255)
                                                                                                                                            cccTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCTGATGACTAAATCTAc     <  2:4312016/149‑1 (MQ=255)
                                                                                                                                            cccTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCTGATGACTAAATCTAc     >  2:2893068/1‑149 (MQ=255)
                                                                                                                                             ccTGCAGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCTGATGACTAAATCTACt    >  2:2449206/1‑149 (MQ=255)
                                                                                                                                              ctgcagTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCTGATGACTAAATCTACtt   <  2:4249601/149‑1 (MQ=255)
                                                                                                                                              ctgcagTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCTGATGACTAAATCTACtt   <  2:2772161/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AACGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCGTCCATTTCATCCATCCGCAGGTCGCTGATGACTAAATCTACTTT  >  CP009273/2681727‑2682018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑