Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,690,643 C→A 9.5% S495* (TCG→TAG)  mltF → membrane‑bound lytic transglycosylase F, murein hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,690,6430CA 9.5% 932.5 / 46.4 266S495* (TCG→TAG) mltFmembrane‑bound lytic transglycosylase F, murein hydrolase
Reads supporting (aligned to +/- strand):  ref base C (123/117);  new base A (9/17);  total (132/134)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

AAGTATCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCC  >  CP009273/2690495‑2690791
                                                                                                                                                    |                                                                                                                                                    
aaGTATCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAgtc                                                                                                                                                      <  2:3756944/149‑1 (MQ=255)
 aGTATCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAgtcg                                                                                                                                                     <  1:1335005/149‑1 (MQ=255)
   tATCAGATTAGCCTGGTGGTTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAgtcgtc                                                                                                                                                   <  1:338978/149‑1 (MQ=255)
   tATCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAgtcgtc                                                                                                                                                   <  2:1698759/149‑1 (MQ=255)
    aTCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGtca                                                                                                                                                  <  1:4395636/149‑1 (MQ=255)
         aTTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCaaa                                                                                                                                             >  2:1536501/1‑149 (MQ=255)
         aTTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCaaa                                                                                                                                             >  2:2917175/1‑149 (MQ=255)
           tAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGTCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACt                                                                                                                                           >  1:9447/1‑149 (MQ=255)
            aGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTa                                                                                                                                          >  1:1567928/1‑149 (MQ=255)
            aGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTa                                                                                                                                          >  1:1567975/1‑149 (MQ=255)
            aGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTAGTCATCAAACTa                                                                                                                                          >  2:2103398/1‑149 (MQ=255)
              ccTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTAtt                                                                                                                                        >  1:1699705/1‑149 (MQ=255)
               cTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTAttt                                                                                                                                       <  2:4207029/149‑1 (MQ=255)
                tggtggGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTg                                                                                                                                      >  2:3688818/1‑149 (MQ=255)
                 ggtggGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGa                                                                                                                                     >  1:3412042/1‑149 (MQ=255)
                     ggTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCa                                                                                                                                 <  2:18208/149‑1 (MQ=255)
                      gTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCAt                                                                                                                                <  2:2604689/149‑1 (MQ=255)
                       ttATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCAtt                                                                                                                               <  1:473682/149‑1 (MQ=255)
                        tATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATtc                                                                                                                              <  1:1364244/149‑1 (MQ=255)
                         aTCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCAGtct                                                                                                                             <  1:2958797/149‑1 (MQ=255)
                          tCTGCAAGAGACAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTAGTCATCAAACTATTTGACCCATtctc                                                                                                                            <  2:2014806/149‑1 (MQ=255)
                          tCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATtctc                                                                                                                            >  2:657434/1‑149 (MQ=255)
                           cTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTcc                                                                                                                           <  2:716690/149‑1 (MQ=255)
                           cTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTcc                                                                                                                           <  2:2360335/149‑1 (MQ=255)
                            tGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTccc                                                                                                                          <  1:1184419/149‑1 (MQ=255)
                               aagagaaagagaAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCctct                                                                                                                       >  1:2672805/1‑149 (MQ=255)
                                agagaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCctctc                                                                                                                      <  2:255220/149‑1 (MQ=255)
                                 gagaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCctctct                                                                                                                     >  1:4269040/1‑149 (MQ=255)
                                 gagaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCctctct                                                                                                                     >  1:1018025/1‑149 (MQ=255)
                                  agaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTctg                                                                                                                    >  2:3486880/1‑149 (MQ=255)
                                   gaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTctgc                                                                                                                   >  2:3027977/1‑149 (MQ=255)
                                   gaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTctgc                                                                                                                   >  1:596120/1‑149 (MQ=255)
                                    aaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTctgct                                                                                                                  <  2:4656825/149‑1 (MQ=255)
                                        agaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGttt                                                                                                              <  1:2913026/149‑1 (MQ=255)
                                        agaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTAGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGttt                                                                                                              <  1:2721162/149‑1 (MQ=255)
                                         gaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGtttt                                                                                                             <  1:4168788/149‑1 (MQ=255)
                                         gaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGtttt                                                                                                             >  1:1123462/1‑149 (MQ=255)
                                             cAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTAGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAg                                                                                                         <  2:1225836/149‑1 (MQ=255)
                                                  tACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAg                                                                                                    <  1:87788/149‑1 (MQ=255)
                                                     aGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGa                                                                                                 <  2:1262743/149‑1 (MQ=255)
                                                      gAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAg                                                                                                <  2:2672805/149‑1 (MQ=255)
                                                      gAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAg                                                                                                >  1:3738917/1‑149 (MQ=255)
                                                         gcggcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGACAGGGAGTGa                                                                                             >  2:619154/1‑149 (MQ=255)
                                                          cggcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGaa                                                                                            <  1:766173/149‑1 (MQ=255)
                                                            gcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAaga                                                                                          >  1:2096836/1‑149 (MQ=255)
                                                                  cAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACa                                                                                    <  1:4198344/149‑1 (MQ=255)
                                                                     cTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACaaaa                                                                                 >  1:4633165/1‑149 (MQ=255)
                                                                     cTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACaaaa                                                                                 <  1:189682/149‑1 (MQ=255)
                                                                      tGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAAt                                                                                <  1:2441271/149‑1 (MQ=255)
                                                                           cAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATc                                                                           <  2:4282732/149‑1 (MQ=255)
                                                                            aGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATcc                                                                          >  2:873227/1‑149 (MQ=255)
                                                                            aGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATcc                                                                          >  1:4080862/1‑149 (MQ=255)
                                                                            aGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATca                                                                          >  1:3331508/1‑148 (MQ=255)
                                                                            aGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTACAGGAAAGGGAGTGAAGAGCAACAAAATTAATcc                                                                          >  2:3261301/1‑149 (MQ=255)
                                                                               aTTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTc                                                                       <  1:1101377/149‑1 (MQ=255)
                                                                               aTTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTc                                                                       <  1:3501614/149‑1 (MQ=255)
                                                                                 tATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGa                                                                     >  2:4714350/1‑149 (MQ=255)
                                                                                 tATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGa                                                                     <  1:4179968/149‑1 (MQ=255)
                                                                                  aTCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAg                                                                    <  2:1878817/149‑1 (MQ=255)
                                                                                  aTCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAg                                                                    <  2:4268325/149‑1 (MQ=255)
                                                                                   tCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAgg                                                                   >  1:2559964/1‑149 (MQ=255)
                                                                                     cggcggTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGAt                                                                 >  1:1957942/1‑149 (MQ=255)
                                                                                      ggcggTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTAGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGAtt                                                                <  1:748600/149‑1 (MQ=255)
                                                                                       gcggTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGCTTg                                                               >  1:326087/1‑149 (MQ=255)
                                                                                       gcggTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTg                                                               >  2:4451499/1‑149 (MQ=255)
                                                                                        cggTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTgc                                                              <  2:3050958/149‑1 (MQ=255)
                                                                                        cggTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTGTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTgc                                                              <  2:284294/149‑1 (MQ=255)
                                                                                           tATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTAGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCt                                                           >  1:3730127/1‑149 (MQ=255)
                                                                                            aTCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCtt                                                          <  2:633840/149‑1 (MQ=255)
                                                                                             tCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCttt                                                         <  2:4370389/149‑1 (MQ=255)
                                                                                                ccTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCtttttt                                                      >  2:3962102/1‑149 (MQ=255)
                                                                                                   aCGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTg                                                   >  2:3144220/1‑149 (MQ=255)
                                                                                                   aCGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTg                                                   >  2:808215/1‑149 (MQ=255)
                                                                                                    cGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTgc                                                  <  1:2258970/149‑1 (MQ=255)
                                                                                                       aGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCt                                               <  1:2869486/149‑1 (MQ=255)
                                                                                                        gTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCtt                                              >  2:199011/1‑149 (MQ=255)
                                                                                                        gTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCtt                                              <  1:3179970/149‑1 (MQ=255)
                                                                                                           gggCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTAGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTaa                                           <  2:2885131/149‑1 (MQ=255)
                                                                                                               aaaGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTc                                       >  1:454217/1‑149 (MQ=255)
                                                                                                                aaGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTcc                                      >  1:1272769/1‑149 (MQ=255)
                                                                                                                 agagAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCAGTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCt                                     <  2:3295033/149‑1 (MQ=255)
                                                                                                                  gagaAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTg                                    >  2:1470393/1‑149 (MQ=255)
                                                                                                                  gagaAATTTCCTATTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTg                                    <  2:391380/149‑1 (MQ=255)
                                                                                                                   agaAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTgg                                   >  1:378352/1‑149 (MQ=255)
                                                                                                                   agaAATGTCCTTTTCTCTCGTTTCTTTCCCAGTAGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTgg                                   <  2:3162188/149‑1 (MQ=255)
                                                                                                                    gaAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTTACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAAAAAAATTAATACGTCGAGGATTTCTCTTTTTTCTGCGCTTTAATTTCATggc                                  >  1:480715/1‑149 (MQ=255)
                                                                                                                      agtttCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTggcgg                                >  2:2510476/3‑149 (MQ=255)
                                                                                                                      aaTTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTggcgg                                <  2:4307175/149‑1 (MQ=255)
                                                                                                                       aTTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTggcggc                               >  2:2776985/1‑149 (MQ=255)
                                                                                                                           cccgttCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCAt                           <  2:3882634/145‑1 (MQ=255)
                                                                                                                                 ctctcGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGaaa                     <  2:4722651/149‑1 (MQ=255)
                                                                                                                                 ctctcGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGaaa                     <  2:543926/149‑1 (MQ=255)
                                                                                                                                 ctctcGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGaaa                     <  2:684632/149‑1 (MQ=255)
                                                                                                                                   ctcGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAaaga                   >  2:1349912/1‑149 (MQ=255)
                                                                                                                                       tttctttcCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTc               >  2:4471634/1‑149 (MQ=255)
                                                                                                                                            ttcCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGa          <  1:1911421/149‑1 (MQ=255)
                                                                                                                                              cccAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGc        <  1:144392/149‑1 (MQ=255)
                                                                                                                                              cccAGTAGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGc        <  2:2294957/149‑1 (MQ=255)
                                                                                                                                                  gtcgtcATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAAcg    >  2:1128312/1‑149 (MQ=255)
                                                                                                                                                   tcgtcATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAAcgc   >  2:1037060/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAGTATCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCC  >  CP009273/2690495‑2690791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑