BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,693,536	C→A	8.0%	L7L (CTG→CTT)	shoB ←	toxic membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,693,536	0	C	A	8.0%	942.2 / 35.8	257	L7L (CTG→CTT)	shoB	toxic membrane protein
Reads supporting (aligned to +/- strand): ref base C (131/105); new base A (11/10); total (142/115)
Fisher's exact test for biased strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

ATATAGTATCTGCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCAGAGCTTTGACGACACCACTCGTTTC  >  CP009273/2693388‑2693684

atataGTATCTGCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCGTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATc                                                                                                                                                      <  2:4460703/149‑1 (MQ=255)

 tataGTATCTGCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTTATGACCCGTTTAATCa                                                                                                                                                     <  1:2201084/149‑1 (MQ=255)

 tataGTATCTGCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCa                                                                                                                                                     >  1:2561902/1‑149 (MQ=255)

 tataGTATCTGCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCa                                                                                                                                                     >  1:3823011/1‑149 (MQ=255)

 tataGTATCTGCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCa                                                                                                                                                     >  1:1718236/1‑149 (MQ=255)

  ataGTATCTGCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAg                                                                                                                                                    >  2:2528889/1‑149 (MQ=255)

    aGTATCTGCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGt                                                                                                                                                  <  1:3600657/149‑1 (MQ=255)

     gTATCTGCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATAAGGTa                                                                                                                                                 <  1:4309187/149‑1 (MQ=255)

       aTCTGCCCAGACACTTATTTATGGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATc                                                                                                                                               >  2:284492/1‑149 (MQ=255)

        tCTGCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCg                                                                                                                                              >  2:932852/1‑149 (MQ=255)

           gCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATAAGGTATCGGCa                                                                                                                                           >  1:1321138/1‑149 (MQ=255)

            cccAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCaa                                                                                                                                          <  1:3727535/149‑1 (MQ=255)

              cAGACACTGATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATc                                                                                                                                        <  1:4460006/149‑1 (MQ=255)

               aGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCa                                                                                                                                       >  2:72100/1‑149 (MQ=255)

               aGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCa                                                                                                                                       <  1:3105398/149‑1 (MQ=255)

                gACACTTATTTATATTTATTAAATTTTCTTCCGACTGGTTCACCGGACGCACCTTAAGTAAGTTTCCTTGTGTTATAAGAACAGAAGGATCATCTGTAAAACATAAATGATGATTTTTATGTACAGTTTAATAAGTTATCGGCAATAAg                                                                                                                                      >  2:3232349/1‑149 (MQ=255)

                gACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAg                                                                                                                                      >  2:3103356/1‑149 (MQ=255)

                gACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAg                                                                                                                                      >  2:4674764/1‑149 (MQ=255)

                gACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAg                                                                                                                                      >  1:2877419/1‑149 (MQ=255)

                 acacTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGt                                                                                                                                     >  2:1428532/1‑149 (MQ=255)

                   acTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCa                                                                                                                                   <  1:718025/149‑1 (MQ=255)

                      tatttatAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTc                                                                                                                                <  2:1242484/149‑1 (MQ=255)

                       atttatAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAAAAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCg                                                                                                                               >  2:2398490/1‑149 (MQ=255)

                        tttatAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGt                                                                                                                              <  2:4174285/149‑1 (MQ=255)

                        tttatAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATGCGt                                                                                                                              >  2:3866218/1‑149 (MQ=255)

                        tttatAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGAAATGATGATTTTGATGACCCGTTTAATCAGGTATAGGCAATCAGTCATTAGt                                                                                                                              >  1:1088414/1‑149 (MQ=255)

                        tttatAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTGTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGt                                                                                                                              <  2:3142107/149‑1 (MQ=255)

                            taGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGttttt                                                                                                                          >  2:659829/1‑149 (MQ=255)

                                tattaAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCtt                                                                                                                      >  1:3671456/1‑149 (MQ=255)

                                 attaAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATAAGGTATCGGCAATCAGTCATTCGTTTTTCCTTa                                                                                                                     <  1:2228940/149‑1 (MQ=255)

                                  ttaAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTaa                                                                                                                    >  2:454655/1‑149 (MQ=255)

                                    aaaGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATAAGGTATCGGCAATCAg                                                                                                                                      <  1:4540475/129‑1 (MQ=255)

                                     aaGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACa                                                                                                                 >  1:985605/1‑149 (MQ=255)

                                      aGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACaa                                                                                                                >  2:2000992/1‑149 (MQ=255)

                                      aGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACaa                                                                                                                >  2:4524730/1‑149 (MQ=255)

                                      aGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACaa                                                                                                                >  2:1374149/1‑149 (MQ=255)

                                       ggTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAg                                                                                                               >  2:3404889/1‑149 (MQ=255)

                                       ggTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAg                                                                                                               <  2:295508/149‑1 (MQ=255)

                                          gCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGaa                                                                                                            >  1:5843/1‑149 (MQ=255)

                                           cGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATAAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAAt                                                                                                           <  1:3990675/149‑1 (MQ=255)

                                            gTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATg                                                                                                          >  1:1874226/1‑149 (MQ=255)

                                                gACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGc                                                                                                      <  1:2381508/149‑1 (MQ=255)

                                                  cTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCa                                                                                                    >  1:3465458/1‑149 (MQ=255)

                                                    ggTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCAtt                                                                                                  <  2:1783394/149‑1 (MQ=255)

                                                    ggTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCAtt                                                                                                  <  2:769451/149‑1 (MQ=255)

                                                         aCCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGAt                                                                                             <  1:3435443/149‑1 (MQ=255)

                                                         aCCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGAt                                                                                             <  2:846708/149‑1 (MQ=255)

                                                          ccGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGAtt                                                                                            <  2:3354696/149‑1 (MQ=255)

                                                          ccGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGAtt                                                                                            >  1:365517/1‑149 (MQ=255)

                                                           cGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTa                                                                                           <  2:2132372/149‑1 (MQ=255)

                                                             gACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGc                                                                                         >  1:4032380/1‑149 (MQ=255)

                                                             gACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGc                                                                                         <  1:1930592/149‑1 (MQ=255)

                                                               cGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCCGTAAAACAGCAATGATGATTTTGATGACCCGTTTCATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGACTGCAGCCATTCAGATTAGccc                                                                                       >  2:2422592/1‑149 (MQ=255)

                                                                 cACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCtt                                                                                     >  1:4651028/1‑149 (MQ=255)

                                                                  aCCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTa                                                                                    <  1:4698045/149‑1 (MQ=255)

                                                                      tAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACAtc                                                                                <  1:682273/149‑1 (MQ=255)

                                                                             gTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCac                                                                         >  2:3743716/1‑148 (MQ=255)

                                                                             gTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCaa                                                                         >  1:775151/1‑149 (MQ=255)

                                                                              tttCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCaaa                                                                        >  1:1817084/1‑149 (MQ=255)

                                                                               ttCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATAAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCaaaa                                                                       >  1:1124877/1‑149 (MQ=255)

                                                                                tCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAAc                                                                      >  2:1579122/1‑149 (MQ=255)

                                                                                   ttGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGa                                                                   <  1:815042/149‑1 (MQ=255)

                                                                                   ttGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGa                                                                   <  1:3278115/149‑1 (MQ=255)

                                                                                   ttGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGa                                                                   <  2:901057/149‑1 (MQ=255)

                                                                                        ttATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATACAGATTAGACCTTACATCTCCCCAAAAATGAAAGTg                                                              >  2:2452006/1‑149 (MQ=255)

                                                                                         tataAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGc                                                             <  2:1990607/149‑1 (MQ=255)

                                                                                         tataAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGc                                                             >  2:3706372/1‑149 (MQ=255)

                                                                                          ataAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCg                                                            <  1:2033012/149‑1 (MQ=255)

                                                                                             aGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGt                                                         >  2:685627/1‑149 (MQ=255)

                                                                                             aGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGt                                                         >  2:3055030/1‑149 (MQ=255)

                                                                                             aGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATAAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGt                                                         >  2:4428517/1‑149 (MQ=255)

                                                                                              gAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGtt                                                        >  1:1280803/1‑149 (MQ=255)

                                                                                              gAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGtt                                                        >  1:1280281/1‑149 (MQ=255)

                                                                                              gAACAGAAGGATCAGCTGTAAAACAGCAATGATGA‑TTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTa                                                       >  2:3991867/1‑149 (MQ=255)

                                                                                                aCAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTAt                                                      <  1:2827336/149‑1 (MQ=255)

                                                                                                  aGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATAAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTg                                                    >  2:515763/1‑149 (MQ=255)

                                                                                                    aaGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAg                                                  <  1:212115/149‑1 (MQ=255)

                                                                                                      ggATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAggg                                                <  1:1444225/149‑1 (MQ=255)

                                                                                                       gATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGt                                               <  1:3825438/149‑1 (MQ=255)

                                                                                                        aTCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTg                                              <  2:3116406/149‑1 (MQ=255)

                                                                                                        aTCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTg                                              >  1:501041/1‑149 (MQ=255)

                                                                                                          cAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCa                                            >  1:839509/1‑149 (MQ=255)

                                                                                                           aGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCAt                                           <  1:3107249/149‑1 (MQ=255)

                                                                                                             cTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCAtgc                                         <  2:2425605/149‑1 (MQ=255)

                                                                                                                 aaaaCAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCa                                     >  2:3560090/1‑149 (MQ=255)

                                                                                                                   aaCAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACt                                   >  2:2811186/1‑149 (MQ=255)

                                                                                                                     cAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTcc                                 >  1:3499565/1‑149 (MQ=255)

                                                                                                                      aGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCca                                >  1:2696948/1‑149 (MQ=255)

                                                                                                                          atgatgATTTTGATGACCCGTTTAATAAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACAcc                            <  2:2920012/149‑1 (MQ=255)

                                                                                                                           tgatgaTTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCa                           <  1:1836760/149‑1 (MQ=255)

                                                                                                                               gatTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCAGAGc                       <  1:2055739/149‑1 (MQ=255)

                                                                                                                                atTTTGATGACCCGTTTAATAAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCAGAGCt                      >  1:2447746/1‑149 (MQ=255)

                                                                                                                                 ttttGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCAGAGCtt                     <  1:3767977/149‑1 (MQ=255)

                                                                                                                                 ttttGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCAGAGCtt                     >  1:2341116/1‑149 (MQ=255)

                                                                                                                                     gatgaCCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCAGAGCTTTgac                 <  2:1874226/149‑1 (MQ=255)

                                                                                                                                      atgaCCCGTTTAATCAGGTATCGGCAATCAGACATTCGTTTATCCTAAAACAAGGAATCCAGCCATTCAGATTAGCCCATACAACTCCCCAAAACTGAACGTGCGAGATATTGAGGGTGCATGCTGCACTCCACACCAGATATTTgacg                >  1:3950298/1‑149 (MQ=255)

                                                                                                                                           ccGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCAGAGCTTTGACGAcacc           >  1:3251622/1‑149 (MQ=255)

                                                                                                                                               ttAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCAGAGCTTTGACGACACCACTc       <  1:2950499/149‑1 (MQ=255)

                                                                                                                                               ttAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCAGAGCTTTGACGACACCACTc       <  1:1395719/149‑1 (MQ=255)

                                                                                                                                                    cAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCAGAGCTTTGACGACACCACTCGTTTc  >  2:1603290/1‑149 (MQ=255)

ATATAGTATCTGCCCAGACACTTATTTATAGTTATTAAAGGTGCGTCCGACTGGTTCACCGGACGCACCTTAAGTACGTTTCCTTGTGTTATAAGAACAGAAGGATCAGCTGTAAAACAGCAATGATGATTTTGATGACCCGTTTAATCAGGTATCGGCAATCAGTCATTCGTTTTTCCTTAAACAAGGAATGCAGCCATTCAGATTAGCCCTTACATCTCCCCAAAACTGAACGTGCGAGTTATTGAGGGTGCATGCTGCACTCCACACCAGAGCTTTGACGACACCACTCGTTTC  >  CP009273/2693388‑2693684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`