Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,694,843 G→T 100% I82I (ATC→ATA)  pdxJ ← pyridoxine 5'‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,694,8430GT99.3% 1029.5 / ‑2.0 301I82I (ATC→ATA) pdxJpyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (168/131);  total (169/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 301 total aligned reads displayed.

AAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCG  >  CP009273/2694698‑2694987
                                                                                                                                                 |                                                                                                                                                
aaaCCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATc                                                                                                                                               >  2:2458176/1‑149 (MQ=255)
 aaCCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCg                                                                                                                                              >  1:4786177/1‑149 (MQ=255)
 aaCCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCg                                                                                                                                              >  1:185831/1‑149 (MQ=255)
  aCCTGAATCCCGGCATCTGCCACATGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGc                                                                                                                                             >  1:1556161/1‑149 (MQ=255)
   ccTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGcc                                                                                                                                            >  1:3246632/1‑149 (MQ=255)
    cTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCa                                                                                                                                           >  2:917895/1‑149 (MQ=255)
     tGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAg                                                                                                                                          >  2:3993592/1‑149 (MQ=255)
        aTCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCcatcat                                                                                                                                       <  2:1772965/149‑1 (MQ=255)
         tCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAtc                                                                                                                                      <  2:3938923/149‑1 (MQ=255)
          cccGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAtct                                                                                                                                     >  1:2954922/1‑149 (MQ=255)
          cccGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAtct                                                                                                                                     >  1:3559468/1‑149 (MQ=255)
          cccGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAtct                                                                                                                                     >  1:3870569/1‑149 (MQ=255)
            cGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATctct                                                                                                                                   >  2:2270748/1‑149 (MQ=255)
             ggCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCtt                                                                                                                                  <  2:3334321/149‑1 (MQ=255)
              gCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTc                                                                                                                                 <  2:1975201/149‑1 (MQ=255)
              gCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTc                                                                                                                                 <  1:840785/149‑1 (MQ=255)
              gCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTc                                                                                                                                 >  1:1310096/1‑149 (MQ=255)
               cATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCg                                                                                                                                >  1:3470057/1‑149 (MQ=255)
                aTCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCgg                                                                                                                               <  1:421510/149‑1 (MQ=255)
                 tCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGt                                                                                                                              >  2:2954728/1‑149 (MQ=255)
                  cTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTc                                                                                                                             <  1:807070/149‑1 (MQ=255)
                  cTGCCAGACGTGTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTc                                                                                                                             <  1:2517766/149‑1 (MQ=255)
                    gCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCAc                                                                                                                           <  1:2167308/149‑1 (MQ=255)
                     ccAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCAcc                                                                                                                          <  2:1283032/149‑1 (MQ=255)
                      cAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCg                                                                                                                         >  2:3867042/1‑149 (MQ=255)
                        gACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGcc                                                                                                                       <  2:1637089/149‑1 (MQ=255)
                        gACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGcc                                                                                                                       >  1:4067433/1‑149 (MQ=255)
                         aCGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCa                                                                                                                      <  2:4043254/149‑1 (MQ=255)
                         aCGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCa                                                                                                                      <  2:4256443/149‑1 (MQ=255)
                          cGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAt                                                                                                                     >  1:4561827/1‑149 (MQ=255)
                          cGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAt                                                                                                                     <  1:1659152/149‑1 (MQ=255)
                            tttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAtct                                                                                                                   >  1:1377138/1‑149 (MQ=255)
                            tttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAtct                                                                                                                   >  2:1315036/1‑149 (MQ=255)
                              tGCAGGCATCGCGCATTTGGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTcc                                                                                                                 >  1:4459571/1‑149 (MQ=255)
                               gCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCa                                                                                                                <  1:2774231/149‑1 (MQ=255)
                               gCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCa                                                                                                                <  2:4277479/149‑1 (MQ=255)
                                 aGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGa                                                                                                              >  1:3423049/1‑149 (MQ=255)
                                 aGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGa                                                                                                              <  1:3706661/149‑1 (MQ=255)
                                  ggCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGAt                                                                                                             >  1:2218424/1‑149 (MQ=255)
                                     aTCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCa                                                                                                          <  1:2693051/149‑1 (MQ=255)
                                     aTCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCa                                                                                                          >  1:3049860/1‑149 (MQ=255)
                                     aTCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCa                                                                                                          <  2:3693626/149‑1 (MQ=255)
                                             ttttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTa                                                                                                  >  2:3256797/1‑149 (MQ=255)
                                                tGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATcc                                                                                               <  1:983893/149‑1 (MQ=255)
                                                  tCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAg                                                                                             >  2:3575539/1‑149 (MQ=255)
                                                  tCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAg                                                                                             <  1:1211895/149‑1 (MQ=255)
                                                      gCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTc                                                                                         <  1:1681062/149‑1 (MQ=255)
                                                          cccTGCGACATCCAGGCCGCCGTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGAc                                                                                     >  2:3227544/1‑149 (MQ=255)
                                                           ccTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACg                                                                                    >  1:2369721/1‑149 (MQ=255)
                                                            cTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGc                                                                                   >  1:4665297/1‑149 (MQ=255)
                                                             tGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCa                                                                                  >  2:183382/1‑149 (MQ=255)
                                                                gACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGGACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGa                                                                               <  1:101715/149‑1 (MQ=255)
                                                                 aCATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGAt                                                                              <  1:1472647/149‑1 (MQ=255)
                                                                 aCATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGAt                                                                              <  2:4492694/149‑1 (MQ=255)
                                                                 aCATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGAt                                                                              >  2:2064583/1‑149 (MQ=255)
                                                                      cAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCa                                                                         >  1:689435/1‑149 (MQ=255)
                                                                       aGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCAc                                                                        <  1:3652060/149‑1 (MQ=255)
                                                                        ggCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACg                                                                       >  1:2251278/1‑149 (MQ=255)
                                                                             ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                                                  >  1:297247/1‑149 (MQ=255)
                                                                             ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                                                  >  1:1985119/1‑149 (MQ=255)
                                                                               ttCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                                                >  1:1831163/1‑149 (MQ=255)
                                                                               ttCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                                                >  1:4566142/1‑149 (MQ=255)
                                                                               ttCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                                                >  2:1212222/1‑149 (MQ=255)
                                                                                    ttgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTa                                                           >  2:3350131/1‑149 (MQ=255)
                                                                                       ttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTCTCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtg                                                        >  2:3174163/1‑149 (MQ=255)
                                                                                       ttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtg                                                        <  2:2711420/149‑1 (MQ=255)
                                                                                        tACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTCTCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtgt                                                       <  2:2666398/149‑1 (MQ=255)
                                                                                          cTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGa                                                     <  2:4142308/149‑1 (MQ=255)
                                                                                            tCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCGTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACg                                                   <  2:4175769/149‑1 (MQ=255)
                                                                                             ccTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACgg                                                  >  1:1081269/1‑149 (MQ=255)
                                                                                              cTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGc                                                 >  1:1189096/1‑149 (MQ=255)
                                                                                                  cGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATc                                             <  2:2777309/149‑1 (MQ=255)
                                                                                                  cGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATc                                             <  2:37916/149‑1 (MQ=255)
                                                                                                    cTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCtt                                           >  1:3990546/1‑149 (MQ=255)
                                                                                                     ttttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTc                                          >  1:4045386/1‑149 (MQ=255)
                                                                                                      tttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCa                                         <  1:3747329/149‑1 (MQ=255)
                                                                                                        tCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACg                                       <  1:2095087/149‑1 (MQ=255)
                                                                                                            gTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTaaa                                   <  1:329905/149‑1 (MQ=255)
                                                                                                             tACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAAt                                  <  1:2598674/149‑1 (MQ=255)
                                                                                                               ccAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGc                                <  2:3422945/149‑1 (MQ=255)
                                                                                                                 aGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCAc                              >  1:2894173/1‑149 (MQ=255)
                                                                                                                  ggCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACg                             >  1:2402195/1‑149 (MQ=255)
                                                                                                                     agcaAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTa                          >  1:1783365/1‑149 (MQ=255)
                                                                                                                     agcaAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTa                          >  2:4556352/1‑149 (MQ=255)
                                                                                                                     agcaAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTa                          <  2:2884131/149‑1 (MQ=255)
                                                                                                                     agcaAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTa                          >  2:1921379/1‑149 (MQ=255)
                                                                                                                     agcaAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTa                          >  1:4171839/1‑149 (MQ=255)
                                                                                                                      gcaAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTaa                         >  1:896061/1‑149 (MQ=255)
                                                                                                                             tgtgGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTc                  <  2:1377138/149‑1 (MQ=255)
                                                                                                                             tgtgGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTc                  <  2:4736983/149‑1 (MQ=255)
                                                                                                                             tgtgGCTTCGTCTCAACGGCTATCGCCAGCATCTCGTCGGTCACCGCCATCTCCAGATTCCTGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTc                  <  2:3534853/149‑1 (MQ=255)
                                                                                                                                ggCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCTCGTAAATGCACGGTAATGCCGTCCGc               >  2:771575/1‑149 (MQ=255)
                                                                                                                                ggCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGc               >  2:2805385/1‑149 (MQ=255)
                                                                                                                                 gCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCt              >  2:3251328/1‑149 (MQ=255)
                                                                                                                                 gCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCt              >  1:3076926/1‑149 (MQ=255)
                                                                                                                                  cTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCACGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTc             <  2:3004712/149‑1 (MQ=255)
                                                                                                                                   ttCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTcc            <  2:1133157/149‑1 (MQ=255)
                                                                                                                                   ttCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTcc            <  2:574329/149‑1 (MQ=255)
                                                                                                                                          cAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGc     >  1:1928491/1‑149 (MQ=255)
                                                                                                                                            aCGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTc   <  2:3470057/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCG  >  CP009273/2694698‑2694987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑