| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,699,380 | C→A | 100% | P368P (CCG→CCT) | lepA ← | back‑translocating elongation factor EF4, GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,699,380 | 0 | C | A | 98.1% | 873.8 / 6.2 | 261 | P368P (CCG→CCT) | lepA | back‑translocating elongation factor EF4, GTPase |
| Reads supporting (aligned to +/- strand): ref base C (3/2); new base A (122/134); total (125/136) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 261 total aligned reads displayed.
TGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGT > CP009273/2699235‑2699525 | tGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGgc < 2:845298/149‑1 (MQ=255)tGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGgc > 2:723502/1‑149 (MQ=255) ccGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGgcgc < 2:1225579/149‑1 (MQ=255) gagaTATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAg > 2:4487927/1‑149 (MQ=255) gagaTATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAg < 1:4394955/149‑1 (MQ=255) gagaTATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAg < 1:2003399/149‑1 (MQ=255) agaTATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgt > 2:4347761/1‑149 (MQ=255) tatGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtggt > 1:1816872/1‑149 (MQ=255) atGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtggtg < 2:918814/149‑1 (MQ=255) atGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtggtg < 2:3891535/149‑1 (MQ=255) atGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtggtg > 1:1017189/1‑149 (MQ=255) atGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtggtg < 1:4296570/149‑1 (MQ=255) gCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGAt < 1:1515039/149‑1 (MQ=255) gCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGAt < 1:892941/149‑1 (MQ=255) gCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGAt < 1:3688198/149‑1 (MQ=255) tGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcag < 2:1126830/149‑1 (MQ=255) gCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcaga < 1:379432/149‑1 (MQ=255) cGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagat < 1:655954/149‑1 (MQ=255) ggCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatc < 2:4075020/149‑1 (MQ=255) gcagcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAGCTTCATACACTACGGTAGGCGCAGTGGTGATcagatcc < 2:3646676/149‑1 (MQ=255) gcagcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatcc > 2:3341565/1‑149 (MQ=255) cagcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatcca < 2:2941271/149‑1 (MQ=255) gcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCg > 2:154322/1‑149 (MQ=255) aTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTa < 1:620670/149‑1 (MQ=255) tgACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTc < 1:1250498/149‑1 (MQ=255) tgACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTc < 1:3739418/149‑1 (MQ=255) acacTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCAc > 1:2978130/1‑149 (MQ=255) acTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGt < 2:2406115/149‑1 (MQ=255) ctctGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGtt > 2:2712677/1‑149 (MQ=255) tctGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTc > 1:316656/1‑149 (MQ=255) cAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTGTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGa < 2:3201899/149‑1 (MQ=255) cAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGa < 2:811621/149‑1 (MQ=255) aaTCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGAc > 1:4638706/1‑149 (MQ=255) cGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCCGATCGTATTCACGTTCCAGACGtt > 1:2077650/1‑149 (MQ=255) gCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTcc < 1:303233/149‑1 (MQ=255) gCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTcc < 1:2999050/149‑1 (MQ=255) cTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCt < 1:4223978/149‑1 (MQ=255) cgcgCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTgg < 2:3423350/149‑1 (MQ=255) gcgcAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGga < 1:444190/149‑1 (MQ=255) gagcAGTTCGTAGATTTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGga < 2:2222769/147‑1 (MQ=255) cgcAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgat < 1:1552162/149‑1 (MQ=255) gcAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgatg > 1:1085777/1‑149 (MQ=255) aGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgatgat < 2:14260/149‑1 (MQ=255) gTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtc > 2:3102447/1‑149 (MQ=255) ttCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtct < 2:1882907/149‑1 (MQ=255) tCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtctc < 1:3644814/149‑1 (MQ=255) gTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCa < 2:4236096/149‑1 (MQ=255) gTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCa > 1:2294893/1‑149 (MQ=255) tAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCAt > 2:3634235/1‑149 (MQ=255) tAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCAt > 1:1280606/1‑149 (MQ=255) gATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACGCTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCAtgt < 1:2818053/149‑1 (MQ=255) tGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGGAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgc < 2:165256/149‑1 (MQ=255) gTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgca < 1:4034486/149‑1 (MQ=255) tATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcagc > 2:1596476/1‑149 (MQ=255) aCCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGcc < 2:4629781/149‑1 (MQ=255) cGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGa < 1:350861/149‑1 (MQ=255) gcaggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAg < 1:4212517/149‑1 (MQ=255) caggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAgg > 1:4610608/1‑149 (MQ=255) ggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGaa < 2:2120491/149‑1 (MQ=255) gCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCg > 1:2249619/1‑149 (MQ=255) ggATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGc > 1:1422933/1‑149 (MQ=255) ggCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGaaa < 1:932494/149‑1 (MQ=255) gCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAAc > 1:4546229/1‑149 (MQ=255) tGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCa > 2:1548959/1‑149 (MQ=255) tGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCa > 2:778886/1‑149 (MQ=255) tCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCaaa < 2:5907/149‑1 (MQ=255) tCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCaaa > 2:4558091/1‑149 (MQ=255) cGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAg > 2:493245/1‑149 (MQ=255) cGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAg < 1:4695489/149‑1 (MQ=255) cGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAg > 2:2886183/1‑149 (MQ=255) gACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc < 1:1828754/149‑1 (MQ=255) gACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc < 1:3406234/149‑1 (MQ=255) gACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc < 1:1952962/149‑1 (MQ=255) aCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGcc < 2:3538106/149‑1 (MQ=255) gTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCa < 2:3768037/149‑1 (MQ=255) gTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCa > 1:1211934/1‑149 (MQ=255) tAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAg < 1:1650231/149‑1 (MQ=255) gATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAGGCCCAgcg > 2:1174660/1‑149 (MQ=255) gATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcg > 2:2002092/1‑149 (MQ=255) aTAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcgc < 1:3300496/149‑1 (MQ=255) aaCTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcgcgc < 2:3804426/149‑1 (MQ=255) aCTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCGGCGCGCt > 2:1652664/1‑149 (MQ=255) cTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTg > 2:2518019/1‑149 (MQ=255) ttGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCtt > 1:3560194/1‑149 (MQ=255) aCGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTcc > 1:4354549/1‑149 (MQ=255) tCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATa < 2:2375942/149‑1 (MQ=255) tCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATa < 2:1707782/149‑1 (MQ=255) aCTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGaa > 2:149300/1‑149 (MQ=255) aCTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGaa > 2:821145/1‑149 (MQ=255) aCTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGaa > 1:4047780/1‑149 (MQ=255) ttCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa > 1:2519702/1‑149 (MQ=255) tCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGGTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAg < 2:790642/149‑1 (MQ=255) cATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGt > 2:2268546/1‑149 (MQ=255) aTACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTg > 2:3270893/1‑149 (MQ=255) aTACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTg > 1:358174/1‑149 (MQ=255) acacTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAg > 2:2328178/1‑149 (MQ=255) acTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGc > 1:533806/1‑149 (MQ=255) tACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCAt < 1:1727307/149‑1 (MQ=255) tACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCAt > 2:4325180/1‑149 (MQ=255) ggTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGt < 1:2372998/149‑1 (MQ=255) | TGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGT > CP009273/2699235‑2699525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |