Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,726,786 C→A 100% A249A (GCG→GCT)  clpB ← protein disaggregation chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,726,7860CA99.6% 920.3 / ‑4.4 268A249A (GCG→GCT) clpBprotein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (139/128);  total (140/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 268 total aligned reads displayed.

ATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTCGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCGGGT  >  CP009273/2726640‑2726931
                                                                                                                                                  |                                                                                                                                                 
aTGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGc                                                                                                                                                 >  2:398607/1‑149 (MQ=255)
  gTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGccc                                                                                                                                               <  2:512138/149‑1 (MQ=255)
   tttCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGcccc                                                                                                                                              <  2:3734751/149‑1 (MQ=255)
   tgtCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGcccc                                                                                                                                              <  2:1346721/147‑1 (MQ=255)
        ggCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGcca                                                                                                                                         >  1:1131211/1‑149 (MQ=255)
          cGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGccacc                                                                                                                                       <  2:256047/149‑1 (MQ=255)
          cGTCCATTGCGCCATCGGCTGTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGccacc                                                                                                                                       <  2:245764/149‑1 (MQ=255)
           gTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGccacca                                                                                                                                      <  2:4218344/149‑1 (MQ=255)
           gTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGccacca                                                                                                                                      <  1:154104/149‑1 (MQ=255)
            tCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAg                                                                                                                                     >  1:3083495/1‑149 (MQ=255)
               aTTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAgcgc                                                                                                                                  >  1:4032350/1‑149 (MQ=255)
               aTTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAgcgc                                                                                                                                  >  1:2665747/1‑149 (MQ=255)
                   cgcCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGccc                                                                                                                              <  2:2724837/149‑1 (MQ=255)
                           gCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAg                                                                                                                      >  1:4268176/1‑149 (MQ=255)
                            cTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAgc                                                                                                                     >  2:2667318/1‑149 (MQ=255)
                             tttACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAgcg                                                                                                                    >  1:574935/1‑149 (MQ=255)
                               tACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGcc                                                                                                                  <  1:465844/149‑1 (MQ=255)
                                aCCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCa                                                                                                                 <  2:1775394/149‑1 (MQ=255)
                                 cccGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAg                                                                                                                <  2:2828600/149‑1 (MQ=255)
                                  ccGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGt                                                                                                               >  1:2428909/1‑149 (MQ=255)
                                     cgcCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTAcc                                                                                                            <  2:2516466/149‑1 (MQ=255)
                                       ccGACCCTGGTATGGAAGTCGTCGAGAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCcg                                                                                                          <  1:1499870/149‑1 (MQ=255)
                                           ccATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCcggcgg                                                                                                      >  1:3443291/1‑149 (MQ=255)
                                            cATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCggcggc                                                                                                     >  1:1949913/1‑149 (MQ=255)
                                             aTGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGcc                                                                                                    >  2:1158034/1‑149 (MQ=255)
                                             aTGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGcc                                                                                                    <  1:3775018/149‑1 (MQ=255)
                                             aTGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGcc                                                                                                    <  1:1385617/149‑1 (MQ=255)
                                               ggTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCtt                                                                                                  <  2:665608/149‑1 (MQ=255)
                                                  aTGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCa                                                                                               <  2:1243587/149‑1 (MQ=255)
                                                   tGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCaa                                                                                              >  2:993865/1‑149 (MQ=255)
                                                    gTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAAc                                                                                             <  2:2598588/149‑1 (MQ=255)
                                                     tAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAAcc                                                                                            >  1:2028897/1‑149 (MQ=255)
                                                      aaTTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAAccc                                                                                           >  1:4581124/1‑149 (MQ=255)
                                                      aaTTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAAccc                                                                                           >  1:4443632/1‑149 (MQ=255)
                                                        ttCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCtt                                                                                         >  1:544606/1‑149 (MQ=255)
                                                        ttCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCtt                                                                                         <  1:330704/149‑1 (MQ=255)
                                                         tcgtcgATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTc                                                                                        <  1:3354585/149‑1 (MQ=255)
                                                            tcgATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCgg                                                                                     <  2:2214074/149‑1 (MQ=255)
                                                             cgATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGc                                                                                    <  2:3962275/149‑1 (MQ=255)
                                                               aTAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCAc                                                                                  >  1:1329724/1‑149 (MQ=255)
                                                                 aaaTAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACtt                                                                                >  1:78592/1‑149 (MQ=255)
                                                                     aGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGcc                                                                            <  1:3159510/149‑1 (MQ=255)
                                                                      ggATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCg                                                                           >  1:355185/1‑149 (MQ=255)
                                                                      ggATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCg                                                                           <  2:3945158/149‑1 (MQ=255)
                                                                      ggATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCg                                                                           >  1:1719238/1‑149 (MQ=255)
                                                                              ttGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGataat                                                                   <  2:2192987/149‑1 (MQ=255)
                                                                              ttGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGataat                                                                   >  2:2127964/1‑149 (MQ=255)
                                                                              ttGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGataat                                                                   <  2:1496994/149‑1 (MQ=255)
                                                                               tGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGataata                                                                  <  2:3224952/149‑1 (MQ=255)
                                                                                gCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATAc                                                                 <  2:3698583/149‑1 (MQ=255)
                                                                                gCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATAc                                                                 <  2:4276645/149‑1 (MQ=255)
                                                                                   ttCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCt                                                              <  2:3264030/149‑1 (MQ=255)
                                                                                   ttCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCt                                                              >  1:3015424/1‑149 (MQ=255)
                                                                                   ttCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCt                                                              <  2:1971226/149‑1 (MQ=255)
                                                                                    tCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTg                                                             >  2:4234955/1‑149 (MQ=255)
                                                                                       tGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTgcgc                                                          >  1:3705450/1‑149 (MQ=255)
                                                                                           tGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGa                                                      <  1:3809274/149‑1 (MQ=255)
                                                                                               aaGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCtt                                                  >  1:1831439/1‑149 (MQ=255)
                                                                                               aaGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCtt                                                  >  1:749331/1‑149 (MQ=255)
                                                                                                  aTCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCaa                                               <  1:1106993/149‑1 (MQ=255)
                                                                                                  aTCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCaa                                               <  1:140511/149‑1 (MQ=255)
                                                                                                  aTCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCaa                                               >  2:4491216/1‑149 (MQ=255)
                                                                                                  aTCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCaa                                               >  2:2093651/1‑149 (MQ=255)
                                                                                                  aTCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCaa                                               <  2:1720351/149‑1 (MQ=255)
                                                                                                    cGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACg                                             >  1:4751279/1‑149 (MQ=255)
                                                                                                     gTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGa                                            >  2:2101615/1‑149 (MQ=255)
                                                                                                      ttAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGAt                                           >  1:3849224/1‑149 (MQ=255)
                                                                                                      ttAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGAt                                           >  1:650440/1‑149 (MQ=255)
                                                                                                      ttAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGAt                                           >  1:954747/1‑149 (MQ=255)
                                                                                                         aGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGc                                        >  2:820120/1‑149 (MQ=255)
                                                                                                             cGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGtt                                    <  1:2127964/149‑1 (MQ=255)
                                                                                                              gCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGttt                                   >  1:3124468/1‑149 (MQ=255)
                                                                                                               ccTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGtttt                                  >  1:2749354/1‑149 (MQ=255)
                                                                                                                cTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTa                                 <  2:1538325/149‑1 (MQ=255)
                                                                                                                cTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTa                                 >  1:3211943/1‑149 (MQ=255)
                                                                                                                 ttttAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTAc                                >  1:880971/1‑149 (MQ=255)
                                                                                                                 ttttAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTAc                                >  2:4589453/1‑149 (MQ=255)
                                                                                                                 ttttAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTAc                                <  1:2230807/149‑1 (MQ=255)
                                                                                                                  tttAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTAcc                               >  1:2216512/1‑149 (MQ=255)
                                                                                                                      aaCGTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACg                           >  1:4360348/1‑149 (MQ=255)
                                                                                                                         gTTCTTCAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCg                        >  1:1668164/1‑149 (MQ=255)
                                                                                                                            cttcAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGt                     >  1:2090475/1‑149 (MQ=255)
                                                                                                                            cttcAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGt                     >  1:2090707/1‑149 (MQ=255)
                                                                                                                            cttcAAACTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGt                     <  2:4337223/149‑1 (MQ=255)
                                                                                                                                aaaCTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCGTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCAc                 <  2:3860336/149‑1 (MQ=255)
                                                                                                                                aaaCTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCAGATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCAc                 <  2:4522445/149‑1 (MQ=255)
                                                                                                                                 aaCTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCAcc                >  1:3022355/1‑149 (MQ=255)
                                                                                                                                   cTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCaa              >  1:2774870/1‑149 (MQ=255)
                                                                                                                                   cTCACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCaa              >  1:2437230/1‑149 (MQ=255)
                                                                                                                                     cACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATc            >  1:1515747/1‑149 (MQ=255)
                                                                                                                                     cACCGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATc            >  1:3503921/1‑149 (MQ=255)
                                                                                                                                       ccGCGATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATCAg          <  1:1940550/149‑1 (MQ=255)
                                                                                                                                        cgcgATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGt         <  2:2949811/149‑1 (MQ=255)
                                                                                                                                        cgcgATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGt         >  1:452562/1‑149 (MQ=255)
                                                                                                                                        cgcgATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGt         <  2:3682043/149‑1 (MQ=255)
                                                                                                                                        cgcgATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGt         >  1:2354651/1‑149 (MQ=255)
                                                                                                                                         gcgATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTa        >  2:3101061/1‑149 (MQ=255)
                                                                                                                                         gcgATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTa        >  1:2500725/1‑149 (MQ=255)
                                                                                                                                           gATATTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTAcc      >  1:1281117/1‑149 (MQ=255)
                                                                                                                                             tatTTAGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCgg    >  2:3888333/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTCGCCCCAGCCACCAGCGCGCCCATATCCAGCGCCAGTACCCGGCGGCCTTTCAACCCTTCCGGCACTTCGCCGTTGATAATACGCTGCGCCAGACCTTCAACGATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCGGGT  >  CP009273/2726640‑2726931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑