BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,771,358	G→A	100%	intergenic (+217/+147)	ypjF → / ← ypjA	CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,771,358	0	G	A	98.9%	921.5 / ‑0.2	276	intergenic (+217/+147)	ypjF/ypjA	CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (148/124); total (151/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 276 total aligned reads displayed.

ACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATG  >  CP009273/2771212‑2771503

aCCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCCATCATGATTGGACAgt                                                                                                                                                 >  1:4072663/1‑149 (MQ=255)

   tcccctccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTa                                                                                                                                              >  1:3423493/1‑149 (MQ=255)

    cccctccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTaa                                                                                                                                             >  1:2577646/1‑149 (MQ=255)

       ctccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGa                                                                                                                                          <  1:4432496/149‑1 (MQ=255)

       ctccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGa                                                                                                                                          <  2:1588736/149‑1 (MQ=255)

        tccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGGTTGGACAGTGTAAGGac                                                                                                                                         >  1:199326/1‑149 (MQ=255)

         cccccGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGAaca                                                                                                                                        <  1:706908/149‑1 (MQ=255)

          ccccGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGacaa                                                                                                                                       >  1:1066156/1‑149 (MQ=255)

              gAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACAcc                                                                                                                                   >  2:3940554/1‑149 (MQ=255)

              gAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACAcc                                                                                                                                   <  2:153623/149‑1 (MQ=255)

                aaaaaCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACcaa                                                                                                                                 <  1:1360951/149‑1 (MQ=255)

                aaaaaCCTGACATTTTCTTTTAGGACCAACAATGGGAACAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACcaa                                                                                                                                 <  2:2519685/149‑1 (MQ=255)

                 aaaaCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACcaac                                                                                                                                >  1:2164515/1‑149 (MQ=255)

                  aaaCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACcaaca                                                                                                                               >  1:241735/1‑149 (MQ=255)

                   aaCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCCATCATGATTGGACAGTGTAAGGACAACACccacaa                                                                                                                              >  2:4462492/1‑149 (MQ=255)

                   aaCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACcaacaa                                                                                                                              <  1:295079/149‑1 (MQ=255)

                    aCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAATTTACACAACCAAGTCACACACCGCCAGCACACCAATCAGGATTGGACAGTGTAAGGACAACACCAACaaa                                                                                                                             >  1:45596/1‑149 (MQ=255)

                    aCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACaaa                                                                                                                             <  1:4644103/149‑1 (MQ=255)

                    aCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACaaa                                                                                                                             >  1:45679/1‑149 (MQ=255)

                        gACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACa                                                                                                                         <  2:512014/149‑1 (MQ=255)

                              ttattttAGTAACAAAAATGTGAACAAAATTAAAATTTAAATGAATATTAAAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGt                                                                                                                   <  2:4524402/146‑1 (MQ=255)

                              ttCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGt                                                                                                                   >  2:4219391/1‑149 (MQ=255)

                               tCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGtt                                                                                                                  <  1:966012/149‑1 (MQ=255)

                                cTTTTAGGACCAACAATGGGACAAAAATGAAAATTGATCTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCATCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTa                                                                                                                 <  2:3859187/149‑1 (MQ=255)

                                 ttttAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAg                                                                                                                >  1:3988413/1‑149 (MQ=255)

                                  tttAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGa                                                                                                               <  1:2110251/149‑1 (MQ=255)

                                  tttAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGa                                                                                                               <  2:323643/149‑1 (MQ=255)

                                   ttAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGaa                                                                                                              <  1:787374/149‑1 (MQ=255)

                                      ggACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGtc                                                                                                           >  1:4711953/1‑149 (MQ=255)

                                      ggACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGtc                                                                                                           <  2:723842/149‑1 (MQ=255)

                                       gACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAAAAAAAACAGGAAGTTAGAAGtct                                                                                                          <  1:214918/149‑1 (MQ=255)

                                          caacaaTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAg                                                                                                       <  1:192488/149‑1 (MQ=255)

                                          caacaaTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAg                                                                                                       >  2:3488717/1‑149 (MQ=255)

                                           aacaaTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGc                                                                                                      <  1:522983/149‑1 (MQ=255)

                                               aTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCaaaa                                                                                                  >  1:2579388/1‑149 (MQ=255)

                                                 gggACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAaca                                                                                                >  2:3786241/1‑149 (MQ=255)

                                                  ggACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAacac                                                                                               <  1:3522849/149‑1 (MQ=255)

                                                    aCCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCACAACACCg                                                                                             >  2:2255772/1‑149 (MQ=255)

                                                    aCCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCg                                                                                             >  1:2675610/1‑149 (MQ=255)

                                                      cAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGAACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGAc                                                                                           >  2:1413342/1‑149 (MQ=255)

                                                       aaaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGAcc                                                                                          <  1:164117/149‑1 (MQ=255)

                                                       aaaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGAcc                                                                                          <  1:4379023/149‑1 (MQ=255)

                                                        aaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCa                                                                                         <  2:310115/149‑1 (MQ=255)

                                                        aaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCa                                                                                         <  2:2142045/149‑1 (MQ=255)

                                                        aaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCa                                                                                         <  2:1091765/149‑1 (MQ=255)

                                                           tGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGAc                                                                                      >  2:3022337/1‑149 (MQ=255)

                                                           tGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGAc                                                                                      >  1:3242283/1‑149 (MQ=255)

                                                            gAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACg                                                                                     >  2:4674652/1‑149 (MQ=255)

                                                             aaaaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACgg                                                                                    <  1:4103672/149‑1 (MQ=255)

                                                                aTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTga                                                                                 <  2:1849615/149‑1 (MQ=255)

                                                                   gAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTgagga                                                                              >  2:814202/1‑149 (MQ=255)

                                                                      cTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGAACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGGGAGGAGAc                                                                           >  2:2579033/1‑149 (MQ=255)

                                                                      cTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCAAGATTGGACAGTGTAAGAACAACACCAACAAAAACAGGAAGTTAGAATTCTCAGCAAAACACCGACCAGACGGTGAGGAGAc                                                                           >  2:185653/1‑149 (MQ=255)

                                                                       tGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACa                                                                          <  1:615618/149‑1 (MQ=255)

                                                                       tGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACa                                                                          >  2:2235458/1‑149 (MQ=255)

                                                                        gAAGATGACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACAt                                                                         <  1:3398274/149‑1 (MQ=255)

                                                                           gATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATaca                                                                      >  2:3349853/1‑147 (MQ=255)

                                                                           gATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATaaa                                                                      >  1:4345577/1‑149 (MQ=255)

                                                                           gATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATaaa                                                                      >  1:1231935/1‑149 (MQ=255)

                                                                             ttACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAg                                                                    >  1:3074920/1‑149 (MQ=255)

                                                                               aCAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGa                                                                  <  1:2806087/149‑1 (MQ=255)

                                                                                caatcaatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGAt                                                                 <  2:2874733/149‑1 (MQ=255)

                                                                                     aatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCa                                                            >  2:3622795/1‑149 (MQ=255)

                                                                                     aatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCa                                                            >  2:4357396/1‑149 (MQ=255)

                                                                                      atTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCaa                                                           <  1:4024305/149‑1 (MQ=255)

                                                                                          aacaacAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGa                                                       <  2:1392050/149‑1 (MQ=255)

                                                                                          aacaacAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGa                                                       >  2:281532/1‑149 (MQ=255)

                                                                                            caacaaGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGc                                                     <  1:2847773/149‑1 (MQ=255)

                                                                                             aacaaGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGcc                                                    >  1:3832931/1‑149 (MQ=255)

                                                                                             aacaaGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGcc                                                    >  2:2322911/1‑149 (MQ=255)

                                                                                                aaGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCcgcg                                                 >  2:3805966/1‑149 (MQ=255)

                                                                                                     acacAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTcc                                            >  2:2011843/1‑149 (MQ=255)

                                                                                                     acacAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTcc                                            <  2:4071316/149‑1 (MQ=255)

                                                                                                      cacaACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCt                                           >  1:3654581/1‑149 (MQ=255)

                                                                                                       acaACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTg                                          >  2:2815105/1‑149 (MQ=255)

                                                                                                        caACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTgg                                         >  1:1509905/1‑149 (MQ=255)

                                                                                                         aaCCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGt                                        <  1:128009/149‑1 (MQ=255)

                                                                                                          aCCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCACAGGAGCCGCGGCTCCTGGTa                                       >  1:2510964/1‑149 (MQ=255)

                                                                                                             aaTTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACa                                    >  1:990373/1‑149 (MQ=255)

                                                                                                             aaTTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCGCCTGGTAACa                                    >  1:140002/1‑149 (MQ=255)

                                                                                                                  aGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGaaa                               <  1:1212230/149‑1 (MQ=255)

                                                                                                                  aGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGaaa                               <  2:2733119/149‑1 (MQ=255)

                                                                                                                     cTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGcc                            <  2:117520/149‑1 (MQ=255)

                                                                                                                       ccGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCca                          <  1:4095159/149‑1 (MQ=255)

                                                                                                                       ccGCCAGCCAACCAATCATGATTGGACAGTGTAAGGACAACACAAACAAAAACAGGAAGTTAGAAGTCTCAGAAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCca                          <  1:4197396/149‑1 (MQ=255)

                                                                                                                         gCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCcaca                        >  2:2525537/1‑149 (MQ=255)

                                                                                                                         gCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCcaca                        <  1:2257584/149‑1 (MQ=255)

                                                                                                                            aGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAt                     >  1:822533/1‑149 (MQ=255)

                                                                                                                             gCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAtg                    >  1:4322792/1‑149 (MQ=255)

                                                                                                                             gCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAtg                    >  1:1933295/1‑149 (MQ=255)

                                                                                                                              cccACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAtgt                   >  1:1327090/1‑149 (MQ=255)

                                                                                                                                  ccaATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGc               >  2:3602533/1‑149 (MQ=255)

                                                                                                                                  ccaATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGc               >  2:3332674/1‑149 (MQ=255)

                                                                                                                                   caATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCt              <  1:3330188/149‑1 (MQ=255)

                                                                                                                                    aaTCATGATTGGACAGTGTAAGGACAACACCAACCAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCtt             <  2:3448482/149‑1 (MQ=255)

                                                                                                                                     aTCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCttt            <  1:112136/149‑1 (MQ=255)

                                                                                                                                       cATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCttttt          <  2:828965/149‑1 (MQ=255)

                                                                                                                                          gATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGt       >  1:883361/1‑149 (MQ=255)

                                                                                                                                          gATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGt       >  1:1829784/1‑149 (MQ=255)

                                                                                                                                               gACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATg  <  2:3678499/149‑1 (MQ=255)

ACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATG  >  CP009273/2771212‑2771503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`